##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_70.34100000006c7b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4336 Sequences flagged as poor quality 0 Sequence length 51 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.46286900369004 38.0 38.0 38.0 32.0 38.0 2 36.45571955719557 38.0 38.0 38.0 32.0 38.0 3 36.492158671586715 38.0 38.0 38.0 32.0 38.0 4 36.775830258302584 38.0 38.0 38.0 32.0 38.0 5 36.60747232472325 38.0 38.0 38.0 32.0 38.0 6 38.79220479704797 40.0 38.0 40.0 38.0 40.0 7 38.367850553505534 40.0 38.0 40.0 38.0 40.0 8 38.479012915129154 40.0 38.0 40.0 38.0 40.0 9 38.55558118081181 40.0 38.0 40.0 38.0 40.0 10 38.27237084870849 40.0 38.0 40.0 38.0 40.0 11 38.44880073800738 40.0 38.0 40.0 38.0 40.0 12 38.11277675276753 40.0 38.0 40.0 38.0 40.0 13 38.455950184501845 40.0 38.0 40.0 38.0 40.0 14 38.293127306273064 40.0 38.0 40.0 38.0 40.0 15 38.31480627306273 40.0 38.0 40.0 38.0 40.0 16 38.1095479704797 40.0 38.0 40.0 32.0 40.0 17 37.771678966789665 40.0 38.0 40.0 32.0 40.0 18 37.74976937269373 40.0 38.0 40.0 32.0 40.0 19 37.97878228782288 40.0 38.0 40.0 32.0 40.0 20 38.111623616236166 40.0 38.0 40.0 32.0 40.0 21 38.1875 40.0 38.0 40.0 38.0 40.0 22 38.32472324723247 40.0 38.0 40.0 38.0 40.0 23 38.30857933579336 40.0 38.0 40.0 38.0 40.0 24 38.16535977859779 40.0 38.0 40.0 32.0 40.0 25 37.83879151291513 40.0 38.0 40.0 32.0 40.0 26 37.884455719557195 40.0 38.0 40.0 32.0 40.0 27 38.00876383763838 40.0 38.0 40.0 38.0 40.0 28 38.013607011070114 40.0 38.0 40.0 32.0 40.0 29 38.00415129151291 40.0 38.0 40.0 32.0 40.0 30 37.98339483394834 40.0 38.0 40.0 32.0 40.0 31 37.936346863468636 40.0 38.0 40.0 32.0 40.0 32 37.615544280442805 40.0 38.0 40.0 32.0 40.0 33 37.86093173431734 40.0 38.0 40.0 32.0 40.0 34 37.92043357933579 40.0 38.0 40.0 32.0 40.0 35 38.00830258302583 40.0 38.0 40.0 38.0 40.0 36 38.15359778597786 40.0 38.0 40.0 38.0 40.0 37 37.72693726937269 40.0 38.0 40.0 32.0 40.0 38 37.82495387453874 40.0 38.0 40.0 32.0 40.0 39 37.46056273062731 40.0 38.0 40.0 32.0 40.0 40 37.40036900369004 40.0 38.0 40.0 32.0 40.0 41 37.71794280442804 40.0 38.0 40.0 32.0 40.0 42 37.79105166051661 40.0 38.0 40.0 32.0 40.0 43 38.13191881918819 40.0 38.0 40.0 38.0 40.0 44 37.8570110701107 40.0 38.0 40.0 32.0 40.0 45 37.923892988929886 40.0 38.0 40.0 32.0 40.0 46 37.59455719557196 40.0 38.0 40.0 32.0 40.0 47 37.612315498154985 40.0 38.0 40.0 32.0 40.0 48 37.503690036900366 40.0 38.0 40.0 32.0 40.0 49 37.31572878228782 40.0 38.0 40.0 32.0 40.0 50 36.99930811808118 38.0 38.0 40.0 32.0 40.0 51 36.646678966789665 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 -0.19913623702685612 2201 2 1.4063810755217006 2201 3 -0.1879562711594005 2201 4 -0.2770708891802727 2201 5 1.1457681641275315 2201 6 1.1598357371794918 2201 7 1.070605913184032 2201 8 1.2161632638195101 2201 9 0.6967457455738639 2201 10 1.4604127643190097 2201 11 0.8237628690753809 2201 12 1.0631893280330829 2201 13 0.7110344950188647 2201 14 0.9954713689088734 2201 15 0.5681767451298683 2201 16 1.0496964840715037 2201 17 -0.23682172861861517 2201 18 0.05406694000443224 2201 19 -0.34594246898934955 2201 20 1.0772724671162166 2201 21 1.1112542405511192 2201 22 0.9059504297785637 2201 23 -0.16905564054001587 2201 24 1.07349599185536 2201 25 1.6650530892718436 2201 26 1.6103725267787823 2201 27 1.9696844821844834 2201 28 1.305228755619389 2201 29 1.2959053966866492 2201 30 -5.198696789321794 2201 31 -0.23737963425463704 2201 32 1.4377197021728279 2201 33 1.1795878600566212 2201 34 1.4403529630092038 2201 35 0.19575220786158098 2201 36 -0.13481470786157956 2201 37 1.9723114125457926 2201 38 1.5125634348290689 2201 39 1.4428750936563404 2201 40 2.086444805194809 2201 41 1.605995870448993 2201 42 1.1309768356643346 2201 43 0.8481355450105426 2201 44 1.2366440850815863 2201 45 1.24219786290098 2201 46 1.3787602588383834 2201 47 1.3792455808080746 2201 48 0.19477553696302863 2201 49 0.06979231705793865 2201 50 1.7652160773254408 2201 51 1.9441211999805788 1101 1 1.3008637629731439 1101 2 0.2063810755216977 1101 3 1.3120437288405995 1101 4 1.2229291108197273 1101 5 1.1457681641275315 1101 6 0.7598357371794933 1101 7 1.570605913184032 1101 8 1.3161632638195115 1101 9 0.39674574557386677 1101 10 1.060412764319011 1101 11 0.9237628690753823 1101 12 1.5631893280330829 1101 13 0.8110344950188662 1101 14 0.6954713689088763 1101 15 1.0681767451298683 1101 16 1.1496964840715052 1101 17 1.7631782713813848 1101 18 1.6540669400044337 1101 19 1.2540575310106519 1101 20 1.177272467116218 1101 21 1.6112542405511192 1101 22 1.005950429778565 1101 23 1.4309443594599855 1101 24 1.1734959918553614 1101 25 1.265053089271845 1101 26 1.2103725267787837 1101 27 1.9696844821844834 1101 28 0.20522875561938747 1101 29 1.3959053966866506 1101 30 1.2513032106782092 1101 31 1.3626203657453644 1101 32 1.9377197021728279 1101 33 1.6795878600566212 1101 34 1.5403529630092052 1101 35 0.9957522078615781 1101 36 0.6651852921384176 1101 37 0.07231141254579398 1101 38 1.6125634348290703 1101 39 1.2428750936563375 1101 40 2.18644480519481 1101 41 2.105995870448993 1101 42 2.1309768356643346 1101 43 1.448135545010544 1101 44 1.7366440850815863 1101 45 1.3421978629009814 1101 46 -1.0212397411616152 1101 47 1.0792455808080774 1101 48 2.1947755369630286 1101 49 1.7697923170579415 1101 50 0.6652160773254394 1101 51 2.54412119998058 2202 1 -1.6991362370268561 2202 2 -0.09361892447829945 2202 3 -1.6879562711594005 2202 4 -3.0270708891802727 2202 5 1.1457681641275315 2202 6 1.1598357371794918 2202 7 -5.679394086815968 2202 8 -5.53383673618049 2202 9 0.19674574557386393 2202 10 -5.78958723568099 2202 11 0.8237628690753809 2202 12 -5.186810671966917 2202 13 -0.7889655049811353 2202 14 -0.5045286310911266 2202 15 0.5681767451298683 2202 16 -0.45030351592849627 2202 17 -4.986821728618615 2202 18 0.05406694000443224 2202 19 1.1540575310106505 2202 20 -0.4227275328837834 2202 21 1.1112542405511192 2202 22 -0.5940495702214363 2202 23 1.3309443594599841 2202 24 -0.42650400814464007 2202 25 1.1650530892718436 2202 26 1.1103725267787823 2202 27 -0.030315517815516557 2202 28 -1.444771244380611 2202 29 -5.954094603313351 2202 30 -1.6986967893217937 2202 31 1.262620365745363 2202 32 1.4377197021728279 2202 33 -2.570412139943379 2202 34 -1.3096470369907962 2202 35 -1.054247792138419 2202 36 1.3651852921384204 2202 37 -0.7776885874542074 2202 38 1.5125634348290689 2202 39 1.4428750936563404 2202 40 1.5864448051948088 2202 41 0.10599587044899295 2202 42 -4.619023164335665 2202 43 -4.901864454989457 2202 44 -1.5133559149184137 2202 45 1.24219786290098 2202 46 0.3787602588383834 2202 47 -0.6207544191919254 2202 48 1.1947755369630286 2202 49 -4.180207682942061 2202 50 1.7652160773254408 2202 51 -4.305878800019421 1102 1 1.3008637629731439 1102 2 1.4063810755217006 1102 3 1.3120437288405995 1102 4 1.2229291108197273 1102 5 1.1457681641275315 1102 6 1.1598357371794918 1102 7 1.570605913184032 1102 8 1.7161632638195101 1102 9 1.196745745573864 1102 10 1.4604127643190097 1102 11 1.3237628690753809 1102 12 1.5631893280330829 1102 13 1.2110344950188647 1102 14 1.4954713689088734 1102 15 1.0681767451298683 1102 16 1.5496964840715037 1102 17 1.7631782713813848 1102 18 2.0540669400044322 1102 19 1.6540575310106505 1102 20 1.5772724671162166 1102 21 1.6112542405511192 1102 22 1.4059504297785637 1102 23 1.8309443594599841 1102 24 1.57349599185536 1102 25 1.6650530892718436 1102 26 1.6103725267787823 1102 27 1.9696844821844834 1102 28 1.805228755619389 1102 29 1.7959053966866492 1102 30 2.0513032106782063 1102 31 1.762620365745363 1102 32 1.9377197021728279 1102 33 1.6795878600566212 1102 34 1.9403529630092038 1102 35 1.5290855411949167 1102 36 1.8651852921384204 1102 37 1.1389780792124569 1102 38 2.012563434829069 1102 39 2.4428750936563404 1102 40 2.586444805194809 1102 41 2.105995870448993 1102 42 2.1309768356643346 1102 43 1.8481355450105426 1102 44 0.4033107517482506 1102 45 1.0755311962343157 1102 46 2.3787602588383834 1102 47 1.8792455808080746 1102 48 2.1947755369630286 1102 49 2.5697923170579386 1102 50 1.5985494106587765 1102 51 2.9441211999805788 2203 1 -4.949136237026856 2203 2 1.4063810755217006 2203 3 -0.1879562711594005 2203 4 -0.2770708891802727 2203 5 -0.35423183587246854 2203 6 -0.8401642628205082 2203 7 1.070605913184032 2203 8 -5.03383673618049 2203 9 0.6967457455738639 2203 10 0.9604127643190097 2203 11 -5.926237130924619 2203 12 -2.686810671966917 2203 13 -5.538965504981135 2203 14 -2.2545286310911266 2203 15 0.5681767451298683 2203 16 1.0496964840715037 2203 17 1.2631782713813848 2203 18 1.5540669400044322 2203 19 1.1540575310106505 2203 20 1.0772724671162166 2203 21 1.1112542405511192 2203 22 0.9059504297785637 2203 23 1.3309443594599841 2203 24 1.57349599185536 2203 25 1.1650530892718436 2203 26 1.1103725267787823 2203 27 1.4696844821844834 2203 28 1.805228755619389 2203 29 -0.20409460331335083 2203 30 1.0513032106782063 2203 31 -4.987379634254637 2203 32 1.4377197021728279 2203 33 1.1795878600566212 2203 34 1.4403529630092038 2203 35 1.695752207861581 2203 36 -0.13481470786157956 2203 37 1.9723114125457926 2203 38 1.5125634348290689 2203 39 0.44287509365634037 2203 40 0.5864448051948088 2203 41 -1.144004129551007 2203 42 -1.1190231643356654 2203 43 -4.901864454989457 2203 44 -1.5133559149184137 2203 45 -0.25780213709902 2203 46 0.3787602588383834 2203 47 1.3792455808080746 2203 48 1.6947755369630286 2203 49 2.0697923170579386 2203 50 -4.484783922674559 2203 51 -3.8058788000194212 1103 1 1.3008637629731439 1103 2 1.4063810755217006 1103 3 1.3120437288405995 1103 4 0.022929110819724485 1103 5 -0.05423183587247138 1103 6 0.7598357371794933 1103 7 1.570605913184032 1103 8 1.3161632638195115 1103 9 1.196745745573864 1103 10 1.4604127643190097 1103 11 1.3237628690753809 1103 12 0.7631893280330857 1103 13 1.2110344950188647 1103 14 0.6954713689088763 1103 15 1.0681767451298683 1103 16 1.1496964840715052 1103 17 1.7631782713813848 1103 18 2.0540669400044322 1103 19 1.6540575310106505 1103 20 1.5772724671162166 1103 21 1.6112542405511192 1103 22 1.005950429778565 1103 23 1.4309443594599855 1103 24 1.57349599185536 1103 25 1.265053089271845 1103 26 1.2103725267787837 1103 27 1.1696844821844863 1103 28 1.4052287556193903 1103 29 1.3959053966866506 1103 30 1.6513032106782077 1103 31 0.9626203657453658 1103 32 1.5377197021728293 1103 33 1.2795878600566226 1103 34 1.5403529630092052 1103 35 1.7957522078615824 1103 36 1.4651852921384219 1103 37 2.072311412545794 1103 38 2.012563434829069 1103 39 2.042875093656342 1103 40 2.586444805194809 1103 41 2.105995870448993 1103 42 2.1309768356643346 1103 43 1.0481355450105454 1103 44 1.3366440850815877 1103 45 1.3421978629009814 1103 46 2.3787602588383834 1103 47 1.479245580808076 1103 48 1.3947755369630315 1103 49 1.7697923170579415 1103 50 1.4652160773254437 1103 51 2.1441211999805816 2204 1 1.3008637629731439 2204 2 -0.5936189244782994 2204 3 0.3120437288405995 2204 4 0.22292911081972733 2204 5 1.1457681641275315 2204 6 0.49316907051282755 2204 7 0.9039392465173677 2204 8 1.0494965971528458 2204 9 0.8634124122405282 2204 10 1.127079430985674 2204 11 0.9904295357420452 2204 12 0.8965226613664186 2204 13 0.5443678283522004 2204 14 -0.17119529775779085 2204 15 0.40151007846320397 2204 16 -3.283636849261832 2204 17 0.09651160471472053 2204 18 -4.112599726662232 2204 19 -0.012609135656013848 2204 20 -0.0893941995504477 2204 21 0.9445875738844549 2204 22 0.7392837631118994 2204 23 0.8309443594599841 2204 24 -0.7598373414779758 2204 25 -3.5016135773948207 2204 26 -3.556294139887882 2204 27 -0.8636488511488523 2204 28 -0.1947712443806111 2204 29 0.12923873001998487 2204 30 0.384636544011542 2204 31 1.0959536990786987 2204 32 -0.06228029782717215 2204 33 1.012921193389957 2204 34 -0.8929803703241319 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1.2229291108197273 1211 5 0.28862530698467737 1211 6 0.8741214514652071 1211 7 1.570605913184032 1211 8 1.7161632638195101 1211 9 1.196745745573864 1211 10 1.174698478604725 1211 11 1.3237628690753809 1211 12 1.5631893280330829 1211 13 1.2110344950188647 1211 14 1.4954713689088734 1211 15 1.0681767451298683 1211 16 0.40683934121435783 1211 17 1.1917496999528154 1211 18 1.7683526542901475 1211 19 1.3683432452963658 1211 20 1.291558181401932 1211 21 0.4683970976939733 1211 22 0.8345218583499943 1211 23 1.2595157880314147 1211 24 1.0020674204267905 1211 25 1.379338803557559 1211 26 1.0389439553502129 1211 27 1.1125416250416293 1211 28 1.805228755619389 1211 29 1.7959053966866492 1211 30 1.479874639249637 1211 31 1.762620365745363 1211 32 1.3662911307442585 1211 33 1.6795878600566212 1211 34 1.654638677294919 1211 35 1.9100379221472963 1211 36 1.5794710064241357 1211 37 0.7580256982600773 1211 38 0.5839920062576383 1211 39 1.5857322365134863 1211 40 2.300730519480524 1211 41 2.105995870448993 1211 42 1.5595482642357652 1211 43 1.5624212592962579 1211 44 1.7366440850815863 1211 45 1.74219786290098 1211 46 2.0930459731240987 1211 47 1.5935312950937899 1211 48 1.6233469655344592 1211 49 1.9983637456293692 1211 50 1.6937875058968714 1211 51 1.2298354856948635 1208 1 -0.07413623702685612 1208 2 0.6563810755217006 1208 3 -0.1879562711594005 1208 4 0.4729291108197273 1208 5 -0.35423183587246854 1208 6 0.15983573717949184 1208 7 0.07060591318403198 1208 8 -5.53383673618049 1208 9 -1.178254254426136 1208 10 -1.6645872356809903 1208 11 0.3237628690753809 1208 12 -2.311810671966917 1208 13 -2.4139655049811353 1208 14 -0.5045286310911266 1208 15 0.31817674512986827 1208 16 -2.0753035159284963 1208 17 -1.8618217286186152 1208 18 -1.5709330599955678 1208 19 0.15405753101065045 1208 20 -2.0477275328837834 1208 21 0.6112542405511192 1208 22 -2.7190495702214363 1208 23 -2.044055640540016 1208 24 0.8234959918553599 1208 25 -0.08494691072815641 1208 26 0.8603725267787823 1208 27 -0.5303155178155166 1208 28 0.3052287556193889 1208 29 0.29590539668664917 1208 30 1.3013032106782063 1208 31 -2.612379634254637 1208 32 -1.9372802978271721 1208 33 -2.945412139943379 1208 34 -0.6846470369907962 1208 35 0.07075220786158098 1208 36 -1.7598147078615796 1208 37 0.34731141254579256 1208 38 -1.8624365651709311 1208 39 1.6928750936563404 1208 40 -1.2885551948051912 1208 41 -3.394004129551007 1208 42 1.3809768356643346 1208 43 0.34813554501054256 1208 44 0.7366440850815863 1208 45 -2.63280213709902 1208 46 1.3787602588383834 1208 47 0.6292455808080746 1208 48 -1.1802244630369714 1208 49 -1.0552076829420614 1208 50 -0.10978392267455916 1208 51 0.31912119998057875 1209 1 -0.4134219513125714 1209 2 0.5492382183788465 1209 3 0.4549008716977454 1209 4 -1.348499460608842 1209 5 1.1457681641275315 1209 6 0.5884071657509224 1209 7 0.9991773417554626 1209 8 -3.5695510218947746 1209 9 -3.803254254426136 1209 10 -0.9681586642524209 1209 11 -1.1048085594960497 1209 12 -0.8653821005383477 1209 13 -1.2175369335525659 1209 14 0.924042797480304 1209 15 -0.646108969155847 1209 16 -0.8788749444999269 1209 17 -0.9511074429043305 1209 18 -0.5173616314241372 1209 19 0.22548610243921985 1209 20 0.148701038544786 1209 21 0.7541113834082651 1209 22 -0.022620998792866942 1209 23 0.97380150231713 1209 24 1.0020674204267905 1209 25 -0.3349469107281564 1209 26 0.4675153839216364 1209 27 -2.458886946386947 1209 28 -0.9090569586663264 1209 29 0.9387625395437951 1209 30 1.479874639249637 1209 31 0.04833465145964766 1209 32 1.0805768450299738 1209 33 1.1081592886280518 1209 34 1.3689243915806344 1209 35 1.6243236364330116 1209 36 -0.5633861364330102 1209 37 1.043739983974362 1209 38 1.4411348634004995 1209 39 -2.271410620629375 1209 40 1.443587662337663 1209 41 1.2488530133061388 1209 42 -0.297594592907096 1209 43 -1.8661501692751727 1209 44 -1.5490702006326984 1209 45 -2.9720878513847353 1209 46 -2.9069540268759013 1209 47 -6.120754419191925 1209 48 -0.23379589160840197 1209 49 -1.0016362543706308 1209 50 -0.44906963696027447 1209 51 -1.7701645143051365 1214 1 -0.4134219513125714 1214 2 0.5492382183788465 1214 3 0.4549008716977454 1214 4 -1.348499460608842 1214 5 -1.425660407301038 1214 6 0.01697859432234594 1214 7 0.7134630560411779 1214 8 -0.4266938790376358 1214 9 -0.6603971115689902 1214 10 -1.3967300928238444 1214 11 -0.3905228452103344 1214 12 0.13461789946165226 1214 13 0.06817735216171883 1214 14 -3.218814345376842 1214 15 -2.503251826298701 1214 16 -0.736017801642781 1214 17 -1.6653931571900458 1214 18 -0.8030759171384219 1214 19 -0.06022818327506485 1214 20 -0.1370132471694987 1214 21 -3.103031473734596 1214 22 -1.1654781416500057 1214 23 0.11665864517426883 1214 24 -3.7122182938589248 1214 25 -4.763518339299587 1214 26 -3.961056044649787 1214 27 -2.744601232101232 1214 28 -0.9090569586663264 1214 29 0.6530482538295033 1214 30 0.337017496392491 1214 31 -0.23737963425463704 1214 32 -0.06228029782717215 1214 33 0.5367307171994753 1214 34 -1.9167898941336503 1214 35 0.19575220786158098 1214 36 1.0080424349955663 1214 37 1.043739983974362 1214 38 0.5839920062576383 1214 39 -0.12855347777222903 1214 40 -2.413555194805191 1214 41 -1.7511469866938612 1214 42 -1.7261660214785195 1214 43 -5.0090073121323115 1214 44 -1.5490702006326984 1214 45 -3.8292307085275894 1214 46 -2.6212397411616166 1214 47 -0.8350401334776407 1214 48 -0.5195101773226867 1214 49 -0.14449339722777665 1214 50 0.5509303630397255 1214 51 -3.7701645143051365 1215 1 0.3008637629731439 1215 2 0.40638107552170055 1215 3 0.3120437288405995 1215 4 0.22292911081972733 1215 5 -0.8542318358724685 1215 6 0.8265024038461561 1215 7 0.9039392465173677 1215 8 0.04949659715284582 1215 9 0.19674574557386393 1215 10 0.2937460976523454 1215 11 0.3237628690753809 1215 12 -0.18681067196691714 1215 13 0.5443678283522004 1215 14 0.8288047022422091 1215 15 0.23484341179653256 1215 16 0.21636315073816803 1215 17 1.0965116047147205 1215 18 0.22073360667109654 1215 19 0.15405753101065045 1215 20 0.5772724671162166 1215 21 0.027920907217783508 1215 22 0.9059504297785637 1215 23 0.4976110261266484 1215 24 0.40682932518869563 1215 25 0.1650530892718436 1215 26 0.193705860112118 1215 27 -0.28031551781551656 1215 28 0.8052287556193889 1215 29 0.9625720633533135 1215 30 0.7179698773448706 1215 31 -0.23737963425463704 1215 32 0.43771970217282785 1215 33 0.8462545267232855 1215 34 1.2736862963425395 1215 35 -1.2209144588050833 1215 36 0.36518529213842044 1215 37 1.3056447458791283 1215 38 1.3458967681624046 1215 39 1.1095417603230047 1215 40 1.9197781385281445 1215 41 1.605995870448993 1215 42 1.297643502330999 1215 43 1.3481355450105426 1215 44 -2.2633559149184137 1215 45 0.74219786290098 1215 46 1.212093592171719 1215 47 1.2125789141414103 1215 48 -1.0552244630369714 1215 49 0.6531256503912743 1215 50 -0.31811725600789487 1215 51 -0.47254546668608555 1212 1 0.015149477258859179 1212 2 -0.6650474959068688 1212 3 -0.4022419854451158 1212 4 0.7943576822482967 1212 5 -1.925660407301038 1212 6 -0.19730711996336225 1212 7 -0.6436798011016833 1212 8 0.07330612096236422 1212 9 -0.16039711156899017 1212 10 -0.6110158071095597 1212 11 0.3237628690753809 1212 12 0.27747504231879816 1212 13 0.06817735216171883 1212 14 -0.07595720251969595 1212 15 -2.146108969155847 1212 16 -0.45030351592849627 1212 17 -0.09396458576146927 1212 18 0.26835265429014754 1212 19 0.36834324529636575 1212 20 -1.708441818598068 1212 21 -0.7458886165917349 1212 22 -0.45119242736429044 1212 23 -1.0976270691114465 1212 24 0.5734959918553599 1212 25 0.236481660700413 1212 26 0.03894395535021289 1212 27 1.1125416250416293 1212 28 0.3766573270479583 1212 29 0.36733396811521857 1212 30 1.0513032106782063 1212 31 -0.8088082056832064 1212 32 -0.705137440684318 1212 33 -1.8204121399433788 1212 34 -1.0596470369907962 1212 35 -0.5899620778527037 1212 36 -1.206243279290149 1212 37 -1.0276885874542074 1212 38 -1.630293708028077 1212 39 -0.9142677634865137 1212 40 -0.1992694805194759 1212 41 -2.751146986693861 1212 42 0.2738339785214805 1212 43 0.34813554501054256 1212 44 0.7366440850815863 1212 45 -0.0435164228133047 1212 46 0.23590311598123748 1212 47 0.5935312950937899 1212 48 0.19477553696302863 1212 49 0.7126494599150845 1212 50 -1.4490696369602745 1212 51 -1.5558788000194212 1213 1 0.7008637629731425 1213 2 -1.6936189244783009 1213 3 0.7120437288405981 1213 4 0.022929110819724485 1213 5 0.54576816412753 1213 6 0.3598357371794947 1213 7 0.7706059131840348 1213 8 0.5161632638195073 1213 9 0.7967457455738653 1213 10 0.8604127643190083 1213 11 0.7237628690753795 1213 12 -1.0368106719669186 1213 13 -0.7889655049811353 1213 14 -0.10452863109112798 1213 15 0.2681767451298711 1213 16 -0.5503035159284977 1213 17 0.563178271381382 1213 18 -0.8459330599955663 1213 19 0.4540575310106476 1213 20 1.177272467116218 1213 21 1.0112542405511178 1213 22 0.6059504297785665 1213 23 1.2309443594599827 1213 24 0.17349599185536135 1213 25 -1.9349469107281578 1213 26 -1.1896274732212149 1213 27 -1.9303155178155151 1213 28 -2.5947712443806097 1213 29 -2.3040946033133523 1213 30 -1.8486967893217923 1213 31 0.16262036574536154 1213 32 0.1377197021728307 1213 33 0.47958786005661835 1213 34 0.9403529630092038 1213 35 1.195752207861581 1213 36 0.46518529213842186 1213 37 -2.627688587454209 1213 38 -0.5874365651709326 1213 39 -0.8571249063436568 1213 40 -3.2135551948051884 1213 41 -2.59400412955101 1213 42 -1.5690231643356682 1213 43 0.6481355450105397 1213 44 0.13664408508158488 1213 45 0.3421978629009814 1213 46 0.3787602588383834 1213 47 -0.4207544191919226 1213 48 -1.0052244630369742 1213 49 -0.1302076829420642 1213 50 -0.434783922674562 1213 51 0.1441211999805816 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 1.0 23 5.0 24 6.0 25 18.0 26 29.0 27 27.0 28 41.0 29 56.0 30 44.0 31 67.0 32 72.0 33 111.0 34 130.0 35 162.0 36 221.0 37 329.0 38 654.0 39 2362.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 5.928489042675894 2.698961937716263 5.836216839677047 85.5363321799308 2 2.8136531365313653 3.0673431734317345 87.68450184501845 6.434501845018451 3 3.89760147601476 5.281365313653136 82.42619926199262 8.394833948339484 4 2.421586715867159 78.57472324723247 13.699261992619927 5.304428044280443 5 2.375461254612546 9.686346863468636 84.66328413284133 3.274907749077491 6 4.612546125461255 86.11623616236163 4.889298892988929 4.381918819188193 7 4.059040590405904 8.994464944649447 7.956642066420665 78.98985239852398 8 3.205719557195572 85.79335793357934 3.8284132841328415 7.172509225092251 9 3.643911439114391 6.526752767527675 4.035977859778598 85.79335793357934 10 3.0673431734317345 84.20202952029521 7.011070110701106 5.719557195571956 11 5.857933579335793 5.719557195571956 79.31273062730627 9.109778597785978 12 3.4132841328413286 8.394833948339484 9.22509225092251 78.96678966789668 13 4.266605166051661 3.4824723247232474 84.36346863468634 7.887453874538745 14 3.8745387453874542 4.082103321033211 6.941881918819188 85.10147601476015 15 5.07380073800738 5.3966789667896675 8.464022140221402 81.06549815498155 16 78.13653136531366 6.180811808118081 4.012915129151291 11.669741697416974 17 8.256457564575646 79.54335793357934 4.035977859778598 8.164206642066421 18 83.34870848708486 6.411439114391144 4.128228782287823 6.111623616236162 19 5.788745387453875 8.994464944649447 4.681734317343174 80.5350553505535 20 9.017527675276753 3.9437269372693726 4.958487084870849 82.08025830258303 21 3.782287822878229 5.719557195571956 5.258302583025831 85.23985239852398 22 4.197416974169742 78.15959409594096 4.404981549815498 13.238007380073801 23 5.142988929889299 5.834870848708487 6.157749077490775 82.86439114391145 24 77.9289667896679 9.778597785977858 5.027675276752768 7.264760147601476 25 7.218634686346863 77.81365313653137 6.226937269372694 8.740774907749078 26 81.41143911439114 8.740774907749078 5.535055350553505 4.312730627306273 27 5.465867158671587 83.0719557195572 5.742619926199262 5.719557195571956 28 9.248154981549815 7.564575645756458 5.465867158671587 77.72140221402213 29 76.86808118081181 11.738929889298893 4.935424354243542 6.4575645756457565 30 6.019372693726937 78.57472324723247 5.465867158671587 9.940036900369003 31 9.686346863468636 8.002767527675276 4.658671586715867 77.65221402214021 32 76.19926199261992 9.640221402214022 4.889298892988929 9.27121771217712 33 5.465867158671587 5.442804428044281 7.080258302583026 82.01107011070111 34 7.310885608856088 6.849630996309964 78.3210332103321 7.5184501845018445 35 3.367158671586716 77.60608856088561 9.017527675276753 10.009225092250922 36 3.1826568265682655 8.002767527675276 84.10977859778598 4.70479704797048 37 3.367158671586716 82.10332103321034 9.22509225092251 5.304428044280443 38 3.5747232472324724 6.203874538745388 83.69464944649447 6.526752767527675 39 3.6900369003690034 8.648523985239851 9.109778597785978 78.55166051660517 40 4.497232472324724 3.920664206642067 82.77214022140221 8.809963099630997 41 6.019372693726937 4.197416974169742 7.264760147601476 82.51845018450184 42 77.2140221402214 4.63560885608856 10.747232472324724 7.4031365313653135 43 5.927121771217712 5.258302583025831 78.71309963099631 10.101476014760149 44 7.472324723247232 77.67527675276753 8.879151291512915 5.973247232472325 45 4.820110701107011 7.172509225092251 82.63376383763837 5.373616236162362 46 77.07564575645756 8.625461254612546 6.80350553505535 7.495387453874539 47 5.673431734317344 4.750922509225092 9.202029520295204 80.37361623616236 48 9.801660516605166 4.174354243542435 4.404981549815498 81.61900369003689 49 77.85977859778598 4.658671586715867 6.619003690036901 10.862546125461254 50 5.604243542435424 4.935424354243542 78.43634686346863 11.023985239852399 51 7.818265682656826 5.004612546125461 8.371771217712178 78.80535055350553 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 1.5 12 2.0 13 1.5 14 1.0 15 1.5 16 2.0 17 1.0 18 0.0 19 0.0 20 0.0 21 0.5 22 1.0 23 2.0 24 3.0 25 2.0 26 1.5 27 2.0 28 5.0 29 8.0 30 11.5 31 15.0 32 16.0 33 17.0 34 18.0 35 19.0 36 28.0 37 37.0 38 33.5 39 30.0 40 31.5 41 33.0 42 40.5 43 48.0 44 51.5 45 55.0 46 53.5 47 52.0 48 111.0 49 170.0 50 263.0 51 356.0 52 1858.0 53 3360.0 54 1710.5 55 61.0 56 43.5 57 26.0 58 19.5 59 13.0 60 11.5 61 10.0 62 7.5 63 5.0 64 4.0 65 3.0 66 1.5 67 0.0 68 1.0 69 2.0 70 1.0 71 0.0 72 0.5 73 1.0 74 0.5 75 1.0 76 2.0 77 1.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023062730627306273 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 4336.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.88191881918819 #Duplication Level Percentage of deduplicated Percentage of total 1 91.53005464480874 15.452029520295202 2 3.825136612021858 1.2915129151291513 3 1.5027322404371584 0.761070110701107 4 0.819672131147541 0.5535055350553505 5 0.6830601092896175 0.5765682656826568 6 0.1366120218579235 0.13837638376383762 7 0.0 0.0 8 0.4098360655737705 0.5535055350553505 9 0.0 0.0 >10 0.546448087431694 1.4990774907749078 >50 0.273224043715847 3.8284132841328415 >100 0.1366120218579235 4.428044280442804 >500 0.0 0.0 >1k 0.1366120218579235 70.91789667896678 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 3075 70.91789667896678 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG 192 4.428044280442804 No Hit TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT 95 2.1909594095940963 No Hit TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT 71 1.6374538745387455 Illumina PCR Primer Index 11 (95% over 21bp) CTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG 19 0.43819188191881914 No Hit CTTATACACATCTGACGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC 18 0.41512915129151295 No Hit CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC 16 0.36900369003690037 No Hit CGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC 12 0.27675276752767525 No Hit CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC 8 0.18450184501845018 No Hit CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAGA 8 0.18450184501845018 No Hit CTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA 8 0.18450184501845018 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 6 0.13837638376383762 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTA 5 0.11531365313653136 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGCTATATTTCGTATGCCGTC 5 0.11531365313653136 No Hit GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA 5 0.11531365313653136 No Hit CTTATACACATCTACGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 5 0.11531365313653136 No Hit GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGA 5 0.11531365313653136 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.023062730627306273 2 0.0 0.0 0.023062730627306273 3 0.0 0.0 0.023062730627306273 4 0.0 0.0 0.046125461254612546 5 0.0 0.0 0.046125461254612546 6 0.0 0.0 0.046125461254612546 7 0.0 0.0 0.046125461254612546 8 0.0 0.0 0.046125461254612546 9 0.0 0.0 0.11531365313653137 10 0.0 0.0 0.11531365313653137 11 0.0 0.0 0.13837638376383765 12 0.0 0.0 0.13837638376383765 13 0.0 0.0 0.13837638376383765 14 0.0 0.0 0.13837638376383765 15 0.0 0.0 0.4381918819188192 16 0.0 0.0 0.5765682656826568 17 0.0 0.0 0.761070110701107 18 0.0 0.0 1.5452029520295203 19 0.0 0.0 2.098708487084871 20 0.0 0.0 2.8136531365313653 21 0.0 0.0 3.0673431734317345 22 0.0 0.0 3.2287822878228782 23 0.0 0.0 3.297970479704797 24 0.0 0.0 3.367158671586716 25 0.0 0.0 3.4132841328413286 26 0.0 0.0 3.4132841328413286 27 0.0 0.0 3.459409594095941 28 0.0 0.0 3.4824723247232474 29 0.0 0.0 3.5516605166051662 30 0.0 0.0 3.643911439114391 31 0.0 0.0 3.8976014760147604 32 0.0 0.0 4.082103321033211 33 0.0 0.0 4.289667896678967 34 0.0 0.0 4.4511070110701105 35 0.0 0.0 4.589483394833948 36 0.0 0.0 5.027675276752768 37 0.0 0.0 5.281365313653136 38 0.0 0.0 5.535055350553505 39 0.0 0.0 5.788745387453875 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 335 0.0 42.985077 45 ATGCCGT 345 0.0 41.739132 44 ACGCTAT 360 0.0 40.625 30 GACGCTA 360 0.0 40.625 29 AGACGAC 360 0.0 40.625 25 GACGACG 360 0.0 40.625 26 CGACGCT 360 0.0 40.625 28 ACGACGC 360 0.0 40.625 27 CGAGACG 360 0.0 40.625 23 GAGACGA 360 0.0 40.625 24 CGCTATA 355 0.0 40.563377 31 GCTATAT 350 0.0 40.500004 32 CTATATC 345 0.0 40.434784 33 ATCTCGT 345 0.0 40.434784 37 TATATCT 340 0.0 40.367645 34 TATCTCG 340 0.0 40.367645 36 ATATCTC 340 0.0 40.367645 35 CACGAGA 370 0.0 40.135136 21 ACGAGAC 370 0.0 40.135136 22 TCTCGTA 350 0.0 39.857143 38 >>END_MODULE