FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_70.34100000006c7b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_70.34100000006c7b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4336
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC307570.91789667896678No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG1924.428044280442804No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT952.1909594095940963No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT711.6374538745387455Illumina PCR Primer Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG190.43819188191881914No Hit
CTTATACACATCTGACGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC180.41512915129151295No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC160.36900369003690037No Hit
CGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC120.27675276752767525No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC80.18450184501845018No Hit
CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAGA80.18450184501845018No Hit
CTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA80.18450184501845018No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC60.13837638376383762No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTA50.11531365313653136No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATTTCGTATGCCGTC50.11531365313653136No Hit
GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA50.11531365313653136No Hit
CTTATACACATCTACGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC50.11531365313653136No Hit
GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGA50.11531365313653136No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3350.042.98507745
ATGCCGT3450.041.73913244
ACGCTAT3600.040.62530
GACGCTA3600.040.62529
AGACGAC3600.040.62525
GACGACG3600.040.62526
CGACGCT3600.040.62528
ACGACGC3600.040.62527
CGAGACG3600.040.62523
GAGACGA3600.040.62524
CGCTATA3550.040.56337731
GCTATAT3500.040.50000432
CTATATC3450.040.43478433
ATCTCGT3450.040.43478437
TATATCT3400.040.36764534
TATCTCG3400.040.36764536
ATATCTC3400.040.36764535
CACGAGA3700.040.13513621
ACGAGAC3700.040.13513622
TCTCGTA3500.039.85714338