##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_7.3410000000688c.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 170746 Sequences flagged as poor quality 0 Sequence length 51 %GC 33 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.62592974359575 38.0 38.0 38.0 32.0 38.0 2 36.52827006196338 38.0 38.0 38.0 32.0 38.0 3 36.53320136342872 38.0 38.0 38.0 32.0 38.0 4 36.56061049746407 38.0 38.0 38.0 32.0 38.0 5 36.553658650861514 38.0 38.0 38.0 32.0 38.0 6 38.52826420531081 40.0 38.0 40.0 38.0 40.0 7 38.50863270589062 40.0 38.0 40.0 38.0 40.0 8 38.44405725463554 40.0 38.0 40.0 38.0 40.0 9 38.492093519028266 40.0 38.0 40.0 38.0 40.0 10 38.39431670434446 40.0 38.0 40.0 38.0 40.0 11 38.34316470078362 40.0 38.0 40.0 38.0 40.0 12 38.3598854438757 40.0 38.0 40.0 38.0 40.0 13 38.26159910041817 40.0 38.0 40.0 38.0 40.0 14 38.26019936045354 40.0 38.0 40.0 38.0 40.0 15 38.190956157098846 40.0 38.0 40.0 38.0 40.0 16 38.14932121396694 40.0 38.0 40.0 38.0 40.0 17 38.24319749803802 40.0 38.0 40.0 38.0 40.0 18 38.1816265095522 40.0 38.0 40.0 38.0 40.0 19 38.253680906141284 40.0 38.0 40.0 38.0 40.0 20 38.279743010085156 40.0 38.0 40.0 38.0 40.0 21 38.244175559017485 40.0 38.0 40.0 38.0 40.0 22 38.30611551661532 40.0 38.0 40.0 38.0 40.0 23 38.22200812903377 40.0 38.0 40.0 38.0 40.0 24 38.17519004837595 40.0 38.0 40.0 38.0 40.0 25 38.101197099785644 40.0 38.0 40.0 32.0 40.0 26 37.85820458458763 40.0 38.0 40.0 32.0 40.0 27 37.65785435676385 40.0 38.0 40.0 32.0 40.0 28 37.39914258606351 40.0 38.0 40.0 32.0 40.0 29 37.07332529019713 40.0 38.0 40.0 32.0 40.0 30 36.64759935811088 40.0 38.0 40.0 27.0 40.0 31 36.19141883265201 40.0 38.0 40.0 27.0 40.0 32 35.65048083117613 40.0 38.0 40.0 27.0 40.0 33 35.053067128951774 38.0 32.0 40.0 13.0 40.0 34 34.410891031122254 38.0 32.0 40.0 13.0 40.0 35 33.70515853958511 38.0 32.0 40.0 13.0 40.0 36 33.00951706042894 38.0 27.0 40.0 13.0 40.0 37 32.37361343750366 38.0 27.0 40.0 13.0 40.0 38 31.65768451383927 38.0 27.0 40.0 13.0 40.0 39 30.95696531690347 38.0 27.0 40.0 13.0 40.0 40 30.352875030747427 38.0 27.0 40.0 13.0 40.0 41 29.736028955290315 38.0 13.0 38.0 13.0 40.0 42 29.182481580827663 32.0 13.0 38.0 13.0 40.0 43 28.645244983777072 32.0 13.0 38.0 13.0 40.0 44 28.167295280709357 32.0 13.0 38.0 13.0 40.0 45 27.732573530273037 32.0 13.0 38.0 13.0 40.0 46 27.302542958546614 27.0 13.0 38.0 13.0 40.0 47 26.94688601782765 27.0 13.0 38.0 13.0 40.0 48 26.546870790530964 27.0 13.0 38.0 13.0 40.0 49 26.28195096810467 27.0 13.0 38.0 13.0 40.0 50 26.036826631370573 27.0 13.0 38.0 13.0 40.0 51 24.699085190868306 27.0 13.0 38.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.19980611209987842 2201 2 0.18945920469217015 2201 3 0.3246814643955318 2201 4 0.05904801840776486 2201 5 0.16467066289718701 2201 6 -0.21873083262646276 2201 7 0.24511273091160035 2201 8 -0.15652819093174486 2201 9 0.024410936351095813 2201 10 0.1255289427810311 2201 11 0.12686375991732746 2201 12 0.16007893656825445 2201 13 -0.12390394252827974 2201 14 0.007938367374684674 2201 15 0.08335347974642815 2201 16 0.4375152123740591 2201 17 0.08424659995934292 2201 18 -0.1597314279569204 2201 19 -0.024661804807195153 2201 20 0.05499571048845553 2201 21 -0.10482613032762345 2201 22 0.3565778749011699 2201 23 0.04886812648040717 2201 24 0.3314140378660966 2201 25 0.26050521388763315 2201 26 0.04114907602933471 2201 27 0.38162145339939 2201 28 0.0769336621982788 2201 29 0.21812221222727857 2201 30 0.5612324250180549 2201 31 0.680199629229449 2201 32 0.6012310712014965 2201 33 -0.23128318973247985 2201 34 -0.5863985513139482 2201 35 -0.4305347921919278 2201 36 -0.15855425157085534 2201 37 -0.17939294734371458 2201 38 -0.36150224517575325 2201 39 0.5052597316035303 2201 40 -0.18897929105036582 2201 41 0.43251607469865405 2201 42 0.24923161561086715 2201 43 0.4538467590830848 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scores pass #Quality Count 14 1.0 15 0.0 16 4.0 17 8.0 18 6.0 19 9.0 20 32.0 21 92.0 22 214.0 23 524.0 24 835.0 25 1545.0 26 2460.0 27 3606.0 28 4914.0 29 6552.0 30 8164.0 31 10477.0 32 12721.0 33 14422.0 34 15552.0 35 15954.0 36 15880.0 37 16352.0 38 19233.0 39 21189.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.09425770882523 23.9423251562857 13.231115355546311 10.732301779342752 2 8.608259196570284 29.0231517543911 30.20621636026074 32.16237268877787 3 35.919435887224296 34.96597284855868 22.978576364892884 6.136014899324143 4 18.004521335785316 17.23788551415553 36.035983273400255 28.7216098766589 5 8.806062806742178 28.94943366169632 43.73806707038525 18.50643646117625 6 41.36670844412168 30.82063415834046 7.898281658135477 19.914375739402388 7 7.661087228983403 41.837583310882835 27.017909643564124 23.483419816569644 8 21.594649362210536 19.6110011361906 33.3237674674663 25.470582034132573 9 17.418856078619704 30.960022489545874 23.640378105490026 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8.933737832804283 23 2.776638984222178 37.64539140009136 44.45609267567029 15.121876940016165 24 3.7511859721457603 45.476321553652795 45.19813055649913 5.574361917702318 25 10.11853864805032 38.43486816675061 42.008011900718024 9.438581284481042 26 3.5960057395531346 39.45357111482035 47.69041553193358 9.260007613692933 27 8.438850690499338 42.40919260187647 46.62481112295456 2.5271455846696265 28 4.729832616869502 40.612371592892366 46.87430452250712 7.783491267731016 29 6.427090532135453 41.16465393039954 46.84326426387734 5.564991273587668 30 8.814726743470953 39.58661567205702 45.58765821116688 6.0109993733051414 31 8.203998922375927 39.59917069799586 41.941831726658314 10.254998652969908 32 11.132325208203998 40.816183102386 40.33945158305319 7.71204010635681 33 10.342848441544751 41.145326976913076 38.78802431682148 9.723800264720696 34 12.45827135042695 42.19132512621086 36.82955969685967 8.520843826502524 35 13.791245475735888 40.90754688250384 35.57447905075375 9.726728591006523 36 14.550267649022524 40.6305272158645 33.995525517435254 10.82367961767772 37 15.301676173966008 40.29142703196561 32.32813653028475 12.078760263783632 38 16.756468672765397 38.640436672015746 32.09269909690418 12.510395558314691 39 18.07772949293102 37.0316142105818 31.33894791093203 13.551708385555154 40 19.291227905778175 36.4933878392466 31.158563011725022 13.056821243250209 41 21.218066601853046 35.61137596195518 29.766436695442355 13.404120740749418 42 21.415435793517858 34.748105372893065 29.406838227542664 14.42962060604641 43 21.526712192379325 34.32291239619084 29.410937884342825 14.739437527087018 44 21.393180513745563 33.0783737247139 29.830274208473405 15.698171553067128 45 20.761247701263866 32.62155482412472 30.19924332048774 16.417954154123667 46 21.721153057758308 32.905016808592876 28.550595621566536 16.823234512082273 47 20.91293500286976 32.893889168706735 28.263619645555387 17.92955618286812 48 20.594333102971664 31.850819345694774 28.48090145596383 19.07394609536973 49 20.3706089747344 31.30790765230225 27.82671336370984 20.49477000925351 50 19.109671676056834 30.530729856043482 27.97371534325841 22.38588312464128 51 19.79910975752606 30.118894225137637 26.315450392409513 23.76654562492679 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 38.0 1 39.0 2 40.0 3 124.0 4 208.0 5 216.5 6 225.0 7 267.0 8 309.0 9 260.0 10 211.0 11 302.5 12 394.0 13 825.5 14 1257.0 15 1721.0 16 2185.0 17 2124.0 18 2063.0 19 2453.5 20 2844.0 21 3910.0 22 4976.0 23 5487.0 24 5998.0 25 6281.0 26 9087.5 27 11611.0 28 12912.0 29 14213.0 30 14084.5 31 13956.0 32 15122.5 33 16289.0 34 17707.5 35 19126.0 36 21272.5 37 23419.0 38 24563.5 39 25708.0 40 19806.0 41 13904.0 42 8244.5 43 2585.0 44 1812.5 45 1040.0 46 735.0 47 430.0 48 398.0 49 366.0 50 466.0 51 566.0 52 335.0 53 104.0 54 69.5 55 35.0 56 26.0 57 17.0 58 16.5 59 16.0 60 15.0 61 14.0 62 10.0 63 6.0 64 6.5 65 7.0 66 6.0 67 5.0 68 4.0 69 3.0 70 3.5 71 4.0 72 2.0 73 0.0 74 2.0 75 2.5 76 1.0 77 2.5 78 4.0 79 2.5 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.038068241715764936 2 0.0017569957714968433 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0017569957714968433 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0017569957714968433 17 0.0011713305143312288 18 0.004099656800159301 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 5.856652571656144E-4 27 0.0 28 0.0 29 0.0 30 0.0052709873144905295 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0035139915429936866 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 170746.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.25921539229142 #Duplication Level Percentage of deduplicated Percentage of total 1 85.64588470046081 31.91098465517826 2 6.642669279827691 4.950012909537146 3 2.4259605577099963 2.7116816085876057 4 1.1853353528308594 1.76658660892891 5 0.7385951229644772 1.3759737387114712 6 0.552605501532753 1.2353788445144442 7 0.3399900863447466 0.886743470085387 8 0.3129370644418224 0.9327831590615393 9 0.2301115789587698 0.7716399196216571 >10 1.4457323487589355 10.571576557222915 >50 0.18344600084904233 4.833451136915323 >100 0.2444585708678905 20.88265151412723 >500 0.038436642979526224 9.936615577876 >1k 0.01383719147262944 7.233920299632084 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACC 2163 1.266793951249224 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATAC 2006 1.1748445058742225 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA 1514 0.8866971993487403 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATA 1385 0.811146381174376 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCA 1207 0.7068979653988966 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 1192 0.6981129865414124 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGAT 1102 0.645403113396507 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1039 0.6085062021950735 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC 1020 0.5973785623089267 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 986 0.5774659435652958 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA 963 0.5639956426504866 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGA 949 0.5557963290501681 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA 917 0.5370550408208684 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA 819 0.4796598456186382 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC 799 0.4679465404753259 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG 764 0.4474482564745294 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTG 762 0.44627692596019813 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 753 0.44100593864570764 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 734 0.42987829875956096 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 698 0.4087943495015988 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCA 696 0.40762301898726766 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATC 696 0.40762301898726766 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTAT 639 0.3742400993288276 No Hit TACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTT 635 0.37189743830016514 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC 588 0.3443711712133813 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTT 585 0.34261417544188444 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 584 0.3420285101847188 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC 560 0.32797254401274406 No Hit TACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT 559 0.3273868787555785 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG 549 0.3215302261839223 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA 532 0.3115739168121069 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTT 531 0.31098825155494125 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA 531 0.31098825155494125 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTG 517 0.3027889379546227 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 490 0.28697597601115105 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAG 489 0.28639031075398547 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA 485 0.28404764972532304 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC 481 0.28170498869666055 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTA 471 0.27584833612500437 No Hit CCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCG 463 0.2711630140676795 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG 456 0.2670633572675202 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAGTACTCTGCGT 446 0.261206704695864 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGT 442 0.2588640436672016 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 442 0.2588640436672016 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG 430 0.2518360605812142 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGT 430 0.2518360605812142 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTA 417 0.24422241223806118 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTC 415 0.24305108172373 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCA 414 0.24246541646656436 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT 409 0.23953709018073632 No Hit CCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTC 408 0.2389514249235707 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACT 403 0.2360230986377426 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCAC 402 0.23543743338057702 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATC 398 0.23309477235191456 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCA 391 0.22899511555175525 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAG 386 0.22606678926592716 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT 380 0.2225527977229335 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCA 377 0.22079580195143667 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTG 376 0.22021013669427103 No Hit ACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGTAC 372 0.21786747566560857 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 364 0.21318215360828363 No Hit ACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGTA 358 0.20966816206528996 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATC 353 0.2067398357794619 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA 350 0.20498284000796504 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 344 0.20146884846497137 No Hit TACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC 338 0.19795485692197767 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCT 332 0.19444086537898397 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCG 330 0.19326953486465276 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTAT 321 0.18799854755016224 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC 315 0.18448455600716854 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGT 315 0.18448455600716854 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTAC 311 0.18214189497850608 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAGTACTCTGCG 310 0.18155622972134047 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC 309 0.18097056446417487 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCAACG 309 0.18097056446417487 No Hit CCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTT 304 0.1780422381783468 No Hit ATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT 291 0.1704285898351938 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGC 284 0.16632893303503452 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC 278 0.16281494149204082 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGG 277 0.16222927623487518 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTAT 269 0.1575439541775503 No Hit ATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC 266 0.15578695840605344 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC 263 0.1540299626345566 No Hit TACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG 261 0.15285863212022535 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTA 259 0.15168730160589414 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGG 257 0.15051597109156292 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA 256 0.1499303058343973 No Hit AAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAA 252 0.14758764480573483 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 252 0.14758764480573483 RNA PCR Primer, Index 27 (95% over 21bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG 251 0.14700197954856922 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACC 250 0.1464163142914036 No Hit AAAAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAA 250 0.1464163142914036 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTC 246 0.14407365326274116 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 234 0.13704567017675376 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTA 232 0.13587433966242254 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCA 227 0.13294601337659448 No Hit ACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGT 227 0.13294601337659448 No Hit CCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT 227 0.13294601337659448 No Hit GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 226 0.13236034811942887 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATC 225 0.13177468286226324 No Hit AAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAA 220 0.12884635657643517 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCA 218 0.12767502606210393 No Hit TACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT 216 0.12650369554777272 No Hit TACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTC 213 0.12474669977627587 No Hit ATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG 211 0.12357536926194464 No Hit GATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT 209 0.12240403874761341 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGT 209 0.12240403874761341 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAGTACTCTGC 209 0.12240403874761341 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTG 208 0.1218183734904478 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCA 207 0.12123270823328218 No Hit GTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAA 201 0.11771871669028851 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTG 198 0.11596172091879164 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTA 198 0.11596172091879164 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC 197 0.11537605566162605 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 194 0.11361905989012919 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGT 192 0.11244772937579797 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGA 190 0.11127639886146674 No Hit GTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAA 190 0.11127639886146674 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAG 189 0.11069073360430112 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTG 186 0.10893373783280429 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAGTACTCTG 186 0.10893373783280429 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACC 181 0.10600541154697622 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAG 178 0.10424841577547936 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 175 0.10249142000398252 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACT 175 0.10249142000398252 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATAC 174 0.10190575474681692 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA 174 0.10190575474681692 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGT 171 0.10014875897532007 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0011713305143312288 5 0.0 0.0 0.0011713305143312288 6 0.0 0.0 0.0011713305143312288 7 0.0 0.0 0.0011713305143312288 8 0.0 0.0 0.0011713305143312288 9 0.0 0.0 0.0011713305143312288 10 0.0 0.0 0.0017569957714968433 11 0.0 0.0 0.0023426610286624576 12 0.0 0.0 0.0023426610286624576 13 0.0 0.0 0.0023426610286624576 14 0.0 0.0 0.004099656800159301 15 0.0 0.0 0.0052709873144905295 16 0.0 0.0 0.0076136483431529876 17 0.0 0.0 0.011127639886146673 18 0.0 0.0 0.01464163142914036 19 0.0 0.0 0.021083949257962118 20 0.0 0.0 0.02518360605812142 21 0.0 0.0 0.029868928115446336 22 0.0 0.0 0.03455425017277125 23 0.0 0.0 0.04392489428742108 24 0.0 0.0 0.04978154685907723 25 0.0 0.0 0.05388120365923653 26 0.0 0.0 0.061494852002389515 27 0.0 0.0 0.07262249188853619 28 0.0 0.0 0.07789347920302672 29 0.0 0.0 0.09136378011783584 30 0.0 0.0 0.10483408103264498 31 0.0 0.0 0.14524498377707237 32 0.0 0.0 0.18214189497850608 33 0.0 0.0 0.22021013669427103 34 0.0 0.0 0.28170498869666055 35 0.0 0.0 0.32797254401274406 36 0.0 0.0 0.39239572230096165 37 0.0 0.0 0.455647570074848 38 0.0 0.0 0.5329553840207091 39 0.0 0.0 0.6254904946528762 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 40 3.4353798E-7 39.374744 45 GTATGCC 45 8.642801E-7 34.99977 42 ATCTCGT 45 8.642801E-7 34.99977 37 CCACGAG 45 8.642801E-7 34.99977 20 TATGCCG 45 8.642801E-7 34.99977 43 CACGAGA 45 8.642801E-7 34.99977 21 CCCACGA 45 8.642801E-7 34.99977 19 TCTCGTA 45 8.642801E-7 34.99977 38 ACGAGAC 45 8.642801E-7 34.99977 22 TATCTCG 45 8.642801E-7 34.99977 36 ATGCCGT 45 8.642801E-7 34.99977 44 CGTATGC 45 8.642801E-7 34.99977 41 TCGTATG 45 8.642801E-7 34.99977 40 CTCGTAT 45 8.642801E-7 34.99977 39 CGAGACG 35 2.810499E-4 32.14265 23 GCCCACG 50 1.9686795E-6 31.499798 18 ACAGAGT 30 0.005135294 29.999807 9 CAACACA 30 0.005135294 29.999807 5 ACACAGA 30 0.005135294 29.999807 7 ATCAACA 30 0.005135294 29.999807 3 >>END_MODULE