FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_7.3410000000688c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_7.3410000000688c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170746
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACC21631.266793951249224No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATAC20061.1748445058742225No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA15140.8866971993487403No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATA13850.811146381174376No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCA12070.7068979653988966No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA11920.6981129865414124No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGAT11020.645403113396507No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10390.6085062021950735No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC10200.5973785623089267No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9860.5774659435652958No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA9630.5639956426504866No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGA9490.5557963290501681No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA9170.5370550408208684No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA8190.4796598456186382No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC7990.4679465404753259No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG7640.4474482564745294No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTG7620.44627692596019813No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7530.44100593864570764No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7340.42987829875956096No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6980.4087943495015988No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCA6960.40762301898726766No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATC6960.40762301898726766No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTAT6390.3742400993288276No Hit
TACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTT6350.37189743830016514No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC5880.3443711712133813No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTT5850.34261417544188444No Hit
ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA5840.3420285101847188No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC5600.32797254401274406No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT5590.3273868787555785No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG5490.3215302261839223No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA5320.3115739168121069No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTT5310.31098825155494125No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA5310.31098825155494125No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTG5170.3027889379546227No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4900.28697597601115105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAG4890.28639031075398547No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA4850.28404764972532304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC4810.28170498869666055No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTA4710.27584833612500437No Hit
CCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCG4630.2711630140676795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG4560.2670633572675202No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAGTACTCTGCGT4460.261206704695864No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGT4420.2588640436672016No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4420.2588640436672016No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG4300.2518360605812142No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGT4300.2518360605812142No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTA4170.24422241223806118No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTC4150.24305108172373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCA4140.24246541646656436No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT4090.23953709018073632No Hit
CCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTC4080.2389514249235707No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACT4030.2360230986377426No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCAC4020.23543743338057702No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATC3980.23309477235191456No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCA3910.22899511555175525No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAG3860.22606678926592716No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT3800.2225527977229335No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCA3770.22079580195143667No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTG3760.22021013669427103No Hit
ACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGTAC3720.21786747566560857No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.21318215360828363No Hit
ACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGTA3580.20966816206528996No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATC3530.2067398357794619No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA3500.20498284000796504No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3440.20146884846497137No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC3380.19795485692197767No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCT3320.19444086537898397No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCG3300.19326953486465276No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTAT3210.18799854755016224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC3150.18448455600716854No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGT3150.18448455600716854No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTAC3110.18214189497850608No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAGTACTCTGCG3100.18155622972134047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC3090.18097056446417487No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCAACG3090.18097056446417487No Hit
CCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTT3040.1780422381783468No Hit
ATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT2910.1704285898351938No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGC2840.16632893303503452No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC2780.16281494149204082No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGG2770.16222927623487518No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTAT2690.1575439541775503No Hit
ATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC2660.15578695840605344No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC2630.1540299626345566No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG2610.15285863212022535No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTA2590.15168730160589414No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGG2570.15051597109156292No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA2560.1499303058343973No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAA2520.14758764480573483No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC2520.14758764480573483RNA PCR Primer, Index 27 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG2510.14700197954856922No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACC2500.1464163142914036No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAA2500.1464163142914036No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTC2460.14407365326274116No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2340.13704567017675376No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTA2320.13587433966242254No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCA2270.13294601337659448No Hit
ACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGT2270.13294601337659448No Hit
CCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT2270.13294601337659448No Hit
GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2260.13236034811942887No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATC2250.13177468286226324No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAA2200.12884635657643517No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCA2180.12767502606210393No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT2160.12650369554777272No Hit
TACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTC2130.12474669977627587No Hit
ATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG2110.12357536926194464No Hit
GATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT2090.12240403874761341No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGT2090.12240403874761341No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAGTACTCTGC2090.12240403874761341No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTG2080.1218183734904478No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCA2070.12123270823328218No Hit
GTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAA2010.11771871669028851No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTG1980.11596172091879164No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTA1980.11596172091879164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC1970.11537605566162605No Hit
GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1940.11361905989012919No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGT1920.11244772937579797No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGA1900.11127639886146674No Hit
GTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAA1900.11127639886146674No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAG1890.11069073360430112No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTG1860.10893373783280429No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAGTACTCTG1860.10893373783280429No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACC1810.10600541154697622No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAG1780.10424841577547936No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.10249142000398252No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACT1750.10249142000398252No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATAC1740.10190575474681692No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA1740.10190575474681692No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGT1710.10014875897532007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC403.4353798E-739.37474445
GTATGCC458.642801E-734.9997742
ATCTCGT458.642801E-734.9997737
CCACGAG458.642801E-734.9997720
TATGCCG458.642801E-734.9997743
CACGAGA458.642801E-734.9997721
CCCACGA458.642801E-734.9997719
TCTCGTA458.642801E-734.9997738
ACGAGAC458.642801E-734.9997722
TATCTCG458.642801E-734.9997736
ATGCCGT458.642801E-734.9997744
CGTATGC458.642801E-734.9997741
TCGTATG458.642801E-734.9997740
CTCGTAT458.642801E-734.9997739
CGAGACG352.810499E-432.1426523
GCCCACG501.9686795E-631.49979818
ACAGAGT300.00513529429.9998079
CAACACA300.00513529429.9998075
ACACAGA300.00513529429.9998077
ATCAACA300.00513529429.9998073
Produced by FastQC (version 0.11.2)