FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_69.34100000006c6e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_69.34100000006c6e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83425
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC20362.440515433023674No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT1450.1738088103086605Illumina PCR Primer Index 11 (95% over 21bp)
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA1020.12226550794126462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT403.4070763E-739.37500445
TGCCGTC2450.037.6530645
GACGCTA2400.037.50000429
GACGACG2400.037.50000426
ACGACGC2400.037.50000427
GCCCACG2550.037.05882318
CTCCGAG2550.037.05882312
TCCGAGC2550.037.05882313
CCGAGCC2550.037.05882314
CGAGCCC2550.037.05882315
TCTCGTA2500.036.89999838
CGACGCT2450.036.73469528
AGACGAC2450.036.73469525
TATCTCG2450.036.73469536
ACGCTAT2450.036.73469530
TCTCCGA2600.036.34615711
ACGAGAC2600.036.34615722
ATCTCGT2550.036.1764737
TATGCCG2550.036.1764743
GAGACGA2550.036.1764724
Produced by FastQC (version 0.11.2)