##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_68.34100000006c52.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3942 Sequences flagged as poor quality 0 Sequence length 51 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.47437848807712 38.0 38.0 38.0 32.0 38.0 2 36.51344495180112 38.0 38.0 38.0 32.0 38.0 3 36.43658041603247 38.0 38.0 38.0 32.0 38.0 4 36.77016742770167 38.0 38.0 38.0 32.0 38.0 5 36.65144596651446 38.0 38.0 38.0 32.0 38.0 6 38.93328259766616 40.0 38.0 40.0 38.0 40.0 7 38.60933536276002 40.0 38.0 40.0 38.0 40.0 8 38.48604769152714 40.0 38.0 40.0 38.0 40.0 9 38.72272957889396 40.0 38.0 40.0 38.0 40.0 10 38.39294774226281 40.0 38.0 40.0 38.0 40.0 11 38.59944190766109 40.0 38.0 40.0 38.0 40.0 12 38.28132927447996 40.0 38.0 40.0 38.0 40.0 13 38.59538305428716 40.0 38.0 40.0 38.0 40.0 14 38.232115677321154 40.0 38.0 40.0 38.0 40.0 15 38.26027397260274 40.0 38.0 40.0 38.0 40.0 16 38.128614916286146 40.0 38.0 40.0 32.0 40.0 17 37.84601725012684 40.0 38.0 40.0 32.0 40.0 18 37.985794013191274 40.0 38.0 40.0 32.0 40.0 19 38.10908168442415 40.0 38.0 40.0 32.0 40.0 20 38.17478437341451 40.0 38.0 40.0 32.0 40.0 21 38.3701166920345 40.0 38.0 40.0 38.0 40.0 22 38.483257229832574 40.0 38.0 40.0 38.0 40.0 23 38.37772704211061 40.0 38.0 40.0 38.0 40.0 24 38.19178082191781 40.0 38.0 40.0 32.0 40.0 25 37.82014205986809 40.0 38.0 40.0 32.0 40.0 26 37.96549974632166 40.0 38.0 40.0 32.0 40.0 27 37.91197361745307 40.0 38.0 40.0 32.0 40.0 28 38.009639776763066 40.0 38.0 40.0 32.0 40.0 29 38.05885337392187 40.0 38.0 40.0 38.0 40.0 30 37.93911719939117 40.0 38.0 40.0 32.0 40.0 31 38.074074074074076 40.0 38.0 40.0 32.0 40.0 32 37.63470319634703 40.0 38.0 40.0 32.0 40.0 33 37.91755454084221 40.0 38.0 40.0 32.0 40.0 34 38.04490106544901 40.0 38.0 40.0 38.0 40.0 35 38.02181633688483 40.0 38.0 40.0 38.0 40.0 36 38.25773718924404 40.0 38.0 40.0 38.0 40.0 37 37.6519533231862 38.0 38.0 40.0 32.0 40.0 38 37.69381024860477 40.0 38.0 40.0 32.0 40.0 39 37.41907661085743 40.0 38.0 40.0 32.0 40.0 40 37.36276002029427 40.0 38.0 40.0 32.0 40.0 41 37.666159309994924 40.0 38.0 40.0 32.0 40.0 42 37.796042617960424 40.0 38.0 40.0 32.0 40.0 43 38.16996448503298 40.0 38.0 40.0 38.0 40.0 44 37.91222729578894 40.0 38.0 40.0 32.0 40.0 45 38.18873668188737 40.0 38.0 40.0 38.0 40.0 46 37.6324200913242 40.0 38.0 40.0 32.0 40.0 47 37.581684424150176 40.0 38.0 40.0 32.0 40.0 48 37.602232369355654 40.0 38.0 40.0 32.0 40.0 49 37.33028919330289 40.0 38.0 40.0 32.0 40.0 50 36.97869101978691 38.0 38.0 40.0 32.0 40.0 51 36.667681380010144 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 0.6516233766233759 2201 2 0.9269209956709963 2201 3 0.9302556818181813 2201 4 0.8081777597402597 2201 5 0.9449235660173141 2201 6 0.7042568542568528 2201 7 0.7831348339160815 2201 8 0.7531674922299896 2201 9 0.703314393939392 2201 10 0.776555215617714 2201 11 0.8914389430014413 2201 12 1.0125439664502185 2201 13 0.8778144598457089 2201 14 0.8926224816849828 2201 15 0.9391335227272712 2201 16 0.35829335594960643 2201 17 1.1460982593795102 2201 18 1.1391188672438686 2201 19 1.0497982052669546 2201 20 0.960022095959598 2201 21 0.9197274080086544 2201 22 0.9016741071428562 2201 23 0.821977588383838 2201 24 1.2443429834054802 2201 25 0.5115485209235215 2201 26 0.4681694173881681 2201 27 0.28789682539682815 2201 28 0.9416351010101032 2201 29 0.8864222582972605 2201 30 -0.4015095147907646 2201 31 0.9947736291486287 2201 32 0.5151503878066377 2201 33 1.1431322150072143 2201 34 1.0129982864357867 2201 35 0.8726709922022415 2201 36 0.9717065920190926 2201 37 1.2544315927128418 2201 38 1.208908279220779 2201 39 1.158067279942279 2201 40 1.1702132936507912 2201 41 1.0668357683982688 2201 42 0.5216405122655106 2201 43 0.9232255591630594 2201 44 1.0808554292929289 2201 45 0.9569981234043752 2201 46 0.570712707431456 2201 47 1.170630411255412 2201 48 0.42135191197691135 2201 49 0.5110829274891762 2201 50 0.7581101190476183 2201 51 -0.4784136002886017 1101 1 0.6516233766233759 1101 2 0.9269209956709963 1101 3 0.9302556818181813 1101 4 0.8081777597402597 1101 5 0.9449235660173141 1101 6 0.2042568542568528 1101 7 0.7831348339160815 1101 8 0.7531674922299896 1101 9 0.703314393939392 1101 10 0.776555215617714 1101 11 0.8914389430014413 1101 12 1.0125439664502185 1101 13 0.8778144598457089 1101 14 0.8926224816849828 1101 15 0.9391335227272712 1101 16 0.5249600226162725 1101 17 1.1460982593795102 1101 18 0.6391188672438686 1101 19 1.0497982052669546 1101 20 0.960022095959598 1101 21 0.4197274080086544 1101 22 0.9016741071428562 1101 23 0.821977588383838 1101 24 1.2443429834054802 1101 25 1.1782151875901876 1101 26 1.1348360840548342 1101 27 0.9545634920634942 1101 28 0.9416351010101032 1101 29 0.8864222582972605 1101 30 0.9318238185425685 1101 31 0.9947736291486287 1101 32 1.1818170544733038 1101 33 1.1431322150072143 1101 34 1.0129982864357867 1101 35 0.37267099220224154 1101 36 0.4717065920190926 1101 37 1.2544315927128418 1101 38 0.708908279220779 1101 39 0.6580672799422791 1101 40 0.6702132936507912 1101 41 1.0668357683982688 1101 42 0.6883071789321766 1101 43 0.9232255591630594 1101 44 1.0808554292929289 1101 45 0.4569981234043752 1101 46 1.237379374098122 1101 47 0.670630411255412 1101 48 1.0880185786435774 1101 49 0.6777495941558422 1101 50 -0.07522321428571566 1101 51 0.021586399711398307 2202 1 0.6516233766233759 2202 2 0.9269209956709963 2202 3 0.9302556818181813 2202 4 0.8081777597402597 2202 5 0.9449235660173141 2202 6 0.7042568542568528 2202 7 0.7831348339160815 2202 8 0.7531674922299896 2202 9 0.203314393939392 2202 10 0.276555215617714 2202 11 0.39143894300144133 2202 12 1.0125439664502185 2202 13 0.37781445984570894 2202 14 0.8926224816849828 2202 15 0.9391335227272712 2202 16 1.0249600226162725 2202 17 0.6460982593795102 2202 18 1.1391188672438686 2202 19 0.5497982052669546 2202 20 0.460022095959598 2202 21 0.4197274080086544 2202 22 0.40167410714285623 2202 23 0.32197758838383805 2202 24 0.7443429834054802 2202 25 -0.8217848124098124 2202 26 0.6348360840548342 2202 27 -0.04543650793650578 2202 28 0.44163510101010317 2202 29 0.3864222582972605 2202 30 0.43182381854256846 2202 31 0.49477362914862866 2202 32 1.1818170544733038 2202 33 1.1431322150072143 2202 34 0.5129982864357867 2202 35 0.8726709922022415 2202 36 -1.0282934079809074 2202 37 -0.7455684072871582 2202 38 0.708908279220779 2202 39 0.6580672799422791 2202 40 0.6702132936507912 2202 41 0.5668357683982688 2202 42 0.6883071789321766 2202 43 0.4232255591630594 2202 44 0.5808554292929289 2202 45 0.9569981234043752 2202 46 0.737379374098122 2202 47 0.670630411255412 2202 48 0.08801857864357743 2202 49 0.6777495941558422 2202 50 0.9247767857142843 2202 51 0.5215863997113983 1102 1 -2.348376623376624 1102 2 0.9269209956709963 1102 3 0.9302556818181813 1102 4 0.8081777597402597 1102 5 0.9449235660173141 1102 6 -0.2957431457431472 1102 7 -0.21686516608391848 1102 8 0.7531674922299896 1102 9 0.703314393939392 1102 10 -0.223444784382286 1102 11 -0.10856105699855867 1102 12 0.012543966450218491 1102 13 0.8778144598457089 1102 14 0.8926224816849828 1102 15 -0.06086647727272876 1102 16 -2.9750399773837275 1102 17 0.14609825937951015 1102 18 -2.8608811327561314 1102 19 0.049798205266954554 1102 20 0.960022095959598 1102 21 0.9197274080086544 1102 22 0.9016741071428562 1102 23 -3.178022411616162 1102 24 -2.75565701659452 1102 25 0.17821518759018762 1102 26 0.13483608405483416 1102 27 -0.04543650793650578 1102 28 -0.05836489898989683 1102 29 -0.11357774170273949 1102 30 -0.06817618145743154 1102 31 0.9947736291486287 1102 32 1.1818170544733038 1102 33 -2.8568677849927857 1102 34 0.01299828643578671 1102 35 -0.12732900779775846 1102 36 -0.028293407980907403 1102 37 0.25443159271284177 1102 38 0.20890827922077904 1102 39 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12 1.0125439664502185 1209 13 0.8778144598457089 1209 14 0.8926224816849828 1209 15 0.6891335227272712 1209 16 1.0249600226162725 1209 17 0.8960982593795102 1209 18 0.6391188672438686 1209 19 0.7997982052669546 1209 20 0.710022095959598 1209 21 -0.08027259199134562 1209 22 0.6516741071428562 1209 23 0.571977588383838 1209 24 1.2443429834054802 1209 25 0.9282151875901876 1209 26 0.6348360840548342 1209 27 0.7045634920634942 1209 28 0.9416351010101032 1209 29 0.6364222582972605 1209 30 0.9318238185425685 1209 31 0.7447736291486287 1209 32 0.9318170544733038 1209 33 0.8931322150072143 1209 34 0.7629982864357867 1209 35 0.6226709922022415 1209 36 0.2217065920190926 1209 37 1.0044315927128418 1209 38 0.958908279220779 1209 39 0.4080672799422791 1209 40 -0.07978670634920881 1209 41 0.31683576839826877 1209 42 0.9383071789321766 1209 43 0.9232255591630594 1209 44 1.0808554292929289 1209 45 0.7069981234043752 1209 46 0.987379374098122 1209 47 0.920630411255412 1209 48 1.0880185786435774 1209 49 1.1777495941558422 1209 50 0.9247767857142843 1209 51 1.0215863997113983 1214 1 0.6516233766233759 1214 2 0.9269209956709963 1214 3 0.9302556818181813 1214 4 0.8081777597402597 1214 5 0.9449235660173141 1214 6 0.7042568542568528 1214 7 0.7831348339160815 1214 8 0.7531674922299896 1214 9 0.703314393939392 1214 10 0.526555215617714 1214 11 0.39143894300144133 1214 12 0.7625439664502185 1214 13 0.6278144598457089 1214 14 0.32119391025641164 1214 15 0.18913352272727124 1214 16 0.024960022616272504 1214 17 0.8960982593795102 1214 18 0.8891188672438686 1214 19 1.0497982052669546 1214 20 0.710022095959598 1214 21 0.6697274080086544 1214 22 0.6516741071428562 1214 23 0.821977588383838 1214 24 0.9943429834054802 1214 25 0.9282151875901876 1214 26 0.8848360840548342 1214 27 0.7045634920634942 1214 28 -0.30836489898989683 1214 29 0.3864222582972605 1214 30 0.6818238185425685 1214 31 0.7447736291486287 1214 32 1.1818170544733038 1214 33 0.8931322150072143 1214 34 0.5129982864357867 1214 35 0.6226709922022415 1214 36 0.4717065920190926 1214 37 0.7544315927128418 1214 38 1.208908279220779 1214 39 1.158067279942279 1214 40 0.9202132936507912 1214 41 1.0668357683982688 1214 42 1.1883071789321766 1214 43 0.4232255591630594 1214 44 0.8308554292929289 1214 45 0.7069981234043752 1214 46 0.987379374098122 1214 47 0.920630411255412 1214 48 0.8380185786435774 1214 49 1.1777495941558422 1214 50 -0.32522321428571566 1214 51 -0.2284136002886017 1215 1 0.10616883116883002 1215 2 0.3814664502164504 1215 3 -1.7970170454545462 1215 4 0.2627232142857139 1215 5 -0.6914400703463226 1215 6 0.3406204906204895 1215 7 -0.6714106206293726 1215 8 0.02589476495726295 1215 9 0.15785984848484702 1215 10 0.049282488344987385 1215 11 -0.38128832972583115 1215 12 0.10345305735931021 1215 13 -0.031276449245199345 1215 14 0.1653497544122562 1215 15 0.5754971590909079 1215 16 0.11586911352536422 1215 17 0.055189168470419325 1215 18 0.7754825036075053 1215 19 0.8679800234487729 1215 20 0.414567550505053 1215 21 0.3742728625541094 1215 22 0.5380377435064929 1215 23 0.45834122474747474 1215 24 0.1534338924963894 1215 25 0.3782151875901869 1215 26 -0.5015275523088025 1215 27 0.04547258297258594 1215 28 0.3961805555555582 1215 29 -0.02266865079364777 1215 30 -0.2681761814574317 1215 31 -0.0052263708513713425 1215 32 0.2727261453823955 1215 33 -0.31141323953823985 1215 34 0.6493619227994234 1215 35 -0.5818744623432126 1215 36 -0.48283886252636155 1215 37 0.16352250180375094 1215 38 0.6634537337662341 1215 39 0.06715818903318826 1215 40 -0.1025139790764813 1215 41 0.15774485930736049 1215 42 0.27921626984126835 1215 43 0.3777710137085144 1215 44 0.35358270202020226 1215 45 0.4115435779498302 1215 46 -0.2171660804473321 1215 47 0.2615395021645037 1215 48 0.08801857864357743 1215 49 0.08684050324675141 1215 50 -0.7570413961038973 1215 51 -0.4784136002886017 1212 1 -0.8483766233766241 1212 2 -1.0730790043290037 1212 3 -0.16065340909090953 1212 4 -0.19182224025974026 1212 5 -1.055076433982686 1212 6 -1.2957431457431472 1212 7 -0.8835318327505854 1212 8 -0.9134991744366774 1212 9 -0.796685606060608 1212 10 -0.723444784382286 1212 11 -0.7752277236652256 1212 12 -0.8207893668831145 1212 13 -0.9555188734876241 1212 14 -0.6073775183150172 1212 15 -0.5154119318181829 1212 16 -1.1568581592019092 1212 17 -1.6872350739538229 1212 18 -1.0275477994227984 1212 19 -1.7835351280663785 1212 20 -0.6399779040404017 1212 21 -0.17118168290043645 1212 22 -0.7346895292207805 1212 23 -1.011355744949495 1212 24 -1.5889903499278528 1212 25 -2.094512085137085 1212 26 -2.198497249278499 1212 27 -1.954527417027415 1212 28 -2.1492739898989877 1212 29 -1.1135777417027395 1212 30 -1.5681761814574315 1212 31 -1.0052263708513713 1212 32 -1.363637490981242 1212 33 -1.5235344516594527 1212 34 -0.9870017135642133 1212 35 -1.4909653714341218 1212 36 -1.0282934079809074 1212 37 -0.9122350739538252 1212 38 -0.6092735389610393 1212 39 -1.341932720057721 1212 40 -1.029786706349209 1212 41 -1.0998308982683982 1212 42 -1.9026019119769142 1212 43 -0.41010777417027366 1212 44 -2.419144570707071 1212 45 -0.8763352099289579 1212 46 -0.9292872925685449 1212 47 0.003963744588745044 1212 48 -1.9119814213564226 1212 49 -1.9131594967532486 1212 50 -1.6863343253968264 1212 51 -1.2284136002886017 1213 1 -1.148376623376624 1213 2 -1.473079004329004 1213 3 -1.0697443181818187 1213 4 -0.5251555735930733 1213 5 -1.055076433982686 1213 6 -0.6290764790764802 1213 7 -0.6168651660839188 1213 8 -0.6468325077700108 1213 9 -0.6966856060606084 1213 10 0.33211077117326937 1213 11 -0.30856105699855885 1213 12 0.41254396645021885 1213 13 0.07781445984570823 1213 14 -0.7073775183150168 1213 15 0.13913352272727053 1213 16 -0.7528177551615052 1213 17 -2.6539017406204897 1213 18 0.028007756132757855 1213 19 -1.1724240169552678 1213 20 -0.8177556818181797 1213 21 0.030838519119765095 1213 22 0.10167410714285552 1213 23 0.021977588383837343 1213 24 -0.5556570165945196 1213 25 0.4282151875901876 1213 26 0.4681694173881681 1213 27 -0.1565476190476165 1213 28 -0.6139204545454522 1213 29 -0.31357774170273967 1213 30 -0.31817618145743154 1213 31 -0.8052263708513712 1213 32 -0.8181829455266962 1213 33 -1.256867784992786 1213 34 -2.098112824675324 1213 35 -0.4606623411310915 1213 36 -0.028293407980907403 1213 37 -0.9455684072871584 1213 38 -0.791091720779221 1213 39 -1.7308216089466102 1213 40 -1.5797867063492088 1213 41 -1.733164231601731 1213 42 -0.011692821067823544 1213 43 -0.8767744408369404 1213 44 -1.3191445707070715 1213 45 -0.04300187659562482 1213 46 -1.3626206259018776 1213 47 -1.496036255411255 1213 48 -0.626267135642137 1213 49 -0.5722504058441578 1213 50 -0.5752232142857157 1213 51 0.021586399711398307 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 1.0 23 2.0 24 4.0 25 8.0 26 18.0 27 30.0 28 27.0 29 43.0 30 53.0 31 65.0 32 77.0 33 83.0 34 115.0 35 162.0 36 209.0 37 316.0 38 574.0 39 2153.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 5.506216696269982 2.0806901801573208 10.454199441766049 81.95889368180664 2 2.5875190258751903 3.1202435312024352 86.70725520040588 7.584982242516489 3 3.65296803652968 7.940131912734652 83.84069000507357 4.5662100456621 4 1.9279553526128868 78.97006595636734 14.434297311009638 4.667681380010147 5 2.384576357179097 13.800101471334347 81.02486047691528 2.7904616945712837 6 4.41400304414003 82.36935565702689 6.316590563165906 6.900050735667174 7 3.3739218670725517 13.470319634703195 4.9974632166412984 78.15829528158295 8 3.4753932014205984 82.03957382039574 3.576864535768645 10.908168442415016 9 3.6783358701166917 10.755961440892946 4.058853373921867 81.5068493150685 10 2.8158295281582952 80.77118214104514 9.715880263825468 6.69710806697108 11 4.388635210553018 6.671740233384068 79.85794013191273 9.081684424150177 12 2.5875190258751903 5.048198883815322 13.191273465246066 79.17300862506342 13 3.9320142059868086 3.2470826991374935 80.97412480974124 11.846778285134448 14 3.881278538812785 3.8559107052257735 7.838660578386605 84.42415017757483 15 8.346017250126838 5.504819888381532 4.084221207508879 82.06494165398274 16 78.20903094875698 8.599695585996955 3.576864535768645 9.614408929477422 17 12.709284627092845 79.1222729578894 3.0441400304414 5.124302384576357 18 79.7818366311517 6.621004566210045 4.236428209030948 9.360730593607306 19 7.077625570776255 5.276509386098427 3.8051750380517504 83.84069000507357 20 5.200405885337392 3.1202435312024352 4.388635210553018 87.29071537290716 21 3.34855403348554 8.37138508371385 4.4647387113140535 83.81532217148656 22 3.5514967021816335 77.44799594114663 5.073566717402334 13.926940639269406 23 7.610350076103501 7.356671740233383 5.200405885337392 79.83257229832572 24 77.49873160832065 9.791983764586504 4.71841704718417 7.9908675799086755 25 11.364789446981227 78.00608828006088 5.403348554033485 5.225773718924404 26 78.9193302891933 12.734652460679857 5.251141552511415 3.0948756976154232 27 6.671740233384068 81.02486047691528 4.4647387113140535 7.838660578386605 28 8.320649416539826 9.233891425672248 4.287163876204972 78.15829528158295 29 76.91527143581938 10.705225773718924 4.26179604261796 8.117706747843734 30 6.8239472349061385 79.3505834601725 3.3739218670725517 10.451547437848808 31 9.309994926433282 8.472856418061896 3.9066463723997966 78.31050228310502 32 76.05276509386098 6.900050735667174 4.033485540334855 13.013698630136986 33 6.341958396752917 4.4393708777270415 10.299340436326737 78.9193302891933 34 4.008117706747844 8.929477422628107 78.259766615931 8.802638254693049 35 2.536783358701167 77.14358193810249 13.444951801116185 6.874682902080162 36 3.0187721968543886 11.263318112633181 80.99949264332827 4.71841704718417 37 2.5621511922881783 79.1222729578894 12.810755961440892 5.504819888381532 38 3.3231861998985286 6.976154236428209 79.9847792998478 9.715880263825468 39 3.8051750380517504 4.693049213597159 12.303399289700659 79.19837645865043 40 4.058853373921867 3.50076103500761 79.7818366311517 12.658548959918821 41 8.37138508371385 3.8051750380517504 7.635717909690512 80.18772196854388 42 77.54946727549468 3.8051750380517504 10.020294266869609 8.625063419583967 43 6.69710806697108 7.838660578386605 77.98072044647387 7.483510908168442 44 4.515474378488077 77.80314561136478 12.02435312024353 5.657026889903602 45 7.052257737189244 8.548959918822932 78.86859462201929 5.530187721968543 46 78.13292744799594 5.580923389142567 7.052257737189244 9.233891425672248 47 7.128361237950279 4.4647387113140535 5.225773718924404 83.18112633181126 48 9.25925925925926 4.388635210553018 3.8559107052257735 82.49619482496195 49 77.87924911212582 4.134956874682902 8.650431253170979 9.335362760020294 50 6.925418569254186 5.048198883815322 78.23439878234399 9.791983764586504 51 4.185692541856925 3.65296803652968 12.430238457635717 79.73110096397768 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 1.0 21 1.5 22 2.0 23 2.0 24 2.0 25 1.5 26 1.0 27 1.0 28 1.0 29 1.0 30 5.0 31 9.0 32 8.0 33 7.0 34 12.0 35 17.0 36 19.0 37 21.0 38 24.0 39 27.0 40 28.5 41 30.0 42 31.0 43 32.0 44 34.5 45 37.0 46 39.5 47 42.0 48 111.0 49 180.0 50 326.0 51 472.0 52 1715.0 53 2958.0 54 1509.5 55 61.0 56 37.0 57 13.0 58 11.0 59 9.0 60 8.5 61 8.0 62 6.0 63 4.0 64 3.0 65 2.0 66 2.5 67 3.0 68 2.0 69 1.0 70 0.5 71 0.0 72 0.0 73 0.0 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025367833587011668 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 3942.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.890918315575849 #Duplication Level Percentage of deduplicated Percentage of total 1 91.14139693356049 13.571790969051243 2 3.577512776831346 1.06544901065449 3 1.0221465076660987 0.45662100456621 4 1.0221465076660987 0.60882800608828 5 0.34071550255536626 0.2536783358701167 6 0.5110732538330494 0.45662100456621 7 0.34071550255536626 0.35514967021816335 8 0.34071550255536626 0.4058853373921867 9 0.34071550255536626 0.45662100456621 >10 0.6814310051107325 1.2683916793505834 >50 0.17035775127768313 1.2937595129375952 >100 0.34071550255536626 8.0416032470827 >500 0.0 0.0 >1k 0.17035775127768313 71.76560121765601 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 2829 71.76560121765601 No Hit TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT 173 4.388635210553018 Illumina PCR Primer Index 11 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT 144 3.65296803652968 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG 51 1.2937595129375952 No Hit CTTATACACATCTGACGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC 14 0.35514967021816335 No Hit ATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCTTC 14 0.35514967021816335 Illumina PCR Primer Index 11 (95% over 23bp) CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAGA 11 0.27904616945712835 No Hit CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACG 11 0.27904616945712835 No Hit CGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC 9 0.228310502283105 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 9 0.228310502283105 No Hit TATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCTT 8 0.20294266869609334 Illumina PCR Primer Index 11 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 8 0.20294266869609334 RNA PCR Primer, Index 27 (95% over 21bp) GTGTATAAGAGACAGCATCTCTTATACACATCTCCGAGCCCACGAGACGAC 7 0.17757483510908167 No Hit GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGA 7 0.17757483510908167 No Hit GAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAC 6 0.15220700152207 No Hit CTTATACACATCTGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG 6 0.15220700152207 No Hit CGTCAGATGTGTATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAC 6 0.15220700152207 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGATATATCTCGTATGCCGTC 5 0.12683916793505834 TruSeq Adapter, Index 2 (95% over 21bp) CTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG 5 0.12683916793505834 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCCCGTATGCCGTC 4 0.10147133434804667 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGCTATATATCGTATGCCGTC 4 0.10147133434804667 No Hit CTCGGAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA 4 0.10147133434804667 No Hit GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA 4 0.10147133434804667 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 4 0.10147133434804667 No Hit CGGAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACG 4 0.10147133434804667 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.025367833587011668 6 0.0 0.0 0.025367833587011668 7 0.0 0.0 0.025367833587011668 8 0.0 0.0 0.025367833587011668 9 0.0 0.0 0.025367833587011668 10 0.0 0.0 0.025367833587011668 11 0.0 0.0 0.050735667174023336 12 0.0 0.0 0.050735667174023336 13 0.0 0.0 0.076103500761035 14 0.0 0.0 0.10147133434804667 15 0.0 0.0 0.532724505327245 16 0.0 0.0 0.7356671740233384 17 0.0 0.0 1.0400811770674785 18 0.0 0.0 1.97869101978691 19 0.0 0.0 2.6382546930492135 20 0.0 0.0 3.0441400304414 21 0.0 0.0 3.069507864028412 22 0.0 0.0 3.145611364789447 23 0.0 0.0 3.1963470319634704 24 0.0 0.0 3.221714865550482 25 0.0 0.0 3.2470826991374935 26 0.0 0.0 3.2470826991374935 27 0.0 0.0 3.2470826991374935 28 0.0 0.0 3.297818366311517 29 0.0 0.0 3.297818366311517 30 0.0 0.0 3.3992897006595637 31 0.0 0.0 3.602232369355657 32 0.0 0.0 3.7037037037037037 33 0.0 0.0 3.95738203957382 34 0.0 0.0 4.185692541856925 35 0.0 0.0 4.439370877727042 36 0.0 0.0 4.616945712836124 37 0.0 0.0 4.845256215119229 38 0.0 0.0 5.0228310502283104 39 0.0 0.0 5.276509386098427 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 305 0.0 45.0 45 ATGCCGT 310 0.0 44.274193 44 GTATGCC 320 0.0 42.89063 42 ATCTCGT 320 0.0 42.89063 37 TATGCCG 320 0.0 42.89063 43 TCTCGTA 320 0.0 42.89063 38 TATCTCG 320 0.0 42.89063 36 CGTATGC 320 0.0 42.89063 41 TCGTATG 320 0.0 42.89063 40 CTCGTAT 320 0.0 42.89063 39 CTATATC 315 0.0 42.85714 33 ACGCTAT 315 0.0 42.85714 30 TATATCT 315 0.0 42.85714 34 CGCTATA 315 0.0 42.85714 31 GCTATAT 315 0.0 42.85714 32 GACGCTA 315 0.0 42.85714 29 GACGACG 315 0.0 42.85714 26 CGACGCT 315 0.0 42.85714 28 ACGACGC 315 0.0 42.85714 27 ATATCTC 315 0.0 42.85714 35 >>END_MODULE