H23NVBCXX l02n01 48h_68.34100000006c52.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 48h_68.34100000006c52.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3942
Sequences flagged as poor quality	0
Sequence length	51
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	2829	71.76560121765601	No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT	173	4.388635210553018	Illumina PCR Primer Index 11 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT	144	3.65296803652968	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG	51	1.2937595129375952	No Hit
CTTATACACATCTGACGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC	14	0.35514967021816335	No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCTTC	14	0.35514967021816335	Illumina PCR Primer Index 11 (95% over 23bp)
CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAGA	11	0.27904616945712835	No Hit
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACG	11	0.27904616945712835	No Hit
CGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC	9	0.228310502283105	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC	9	0.228310502283105	No Hit
TATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCTT	8	0.20294266869609334	Illumina PCR Primer Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	8	0.20294266869609334	RNA PCR Primer, Index 27 (95% over 21bp)
GTGTATAAGAGACAGCATCTCTTATACACATCTCCGAGCCCACGAGACGAC	7	0.17757483510908167	No Hit
GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGA	7	0.17757483510908167	No Hit
GAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAC	6	0.15220700152207	No Hit
CTTATACACATCTGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG	6	0.15220700152207	No Hit
CGTCAGATGTGTATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAC	6	0.15220700152207	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGATATATCTCGTATGCCGTC	5	0.12683916793505834	TruSeq Adapter, Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG	5	0.12683916793505834	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCCCGTATGCCGTC	4	0.10147133434804667	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATATCGTATGCCGTC	4	0.10147133434804667	No Hit
CTCGGAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA	4	0.10147133434804667	No Hit
GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA	4	0.10147133434804667	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	4	0.10147133434804667	No Hit
CGGAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACG	4	0.10147133434804667	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	305	45.0	45
ATGCCGT	310	44.274193	44
GTATGCC	320	42.89063	42
ATCTCGT	320	42.89063	37
TATGCCG	320	42.89063	43
TCTCGTA	320	42.89063	38
TATCTCG	320	42.89063	36
CGTATGC	320	42.89063	41
TCGTATG	320	42.89063	40
CTCGTAT	320	42.89063	39
CTATATC	315	42.85714	33
ACGCTAT	315	42.85714	30
TATATCT	315	42.85714	34
CGCTATA	315	42.85714	31
GCTATAT	315	42.85714	32
GACGCTA	315	42.85714	29
GACGACG	315	42.85714	26
CGACGCT	315	42.85714	28
ACGACGC	315	42.85714	27
ATATCTC	315	42.85714	35

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content