FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_67.34100000006c45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_67.34100000006c45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66594
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC5250.7883593116496982No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.18019641409135959No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.151665315193561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT351.1890188E-745.00000439
TATGCCG351.1890188E-745.00000443
TATCTCG351.1890188E-745.00000436
CGCTATA351.1890188E-745.00000431
TCTCGTA351.1890188E-745.00000438
ACGCTAT351.1890188E-745.00000430
TGCCGTC351.1890188E-745.00000445
ATCTCGT403.3932338E-739.37537
GCCCACG403.3932338E-739.37518
CTCCGAG403.3932338E-739.37512
CGACGCT403.3932338E-739.37528
CTATATC403.3932338E-739.37533
CACGAGA403.3932338E-739.37521
ATATCTC403.3932338E-739.37535
AGACGAC403.3932338E-739.37525
GTATGCC403.3932338E-739.37542
TCGTATG403.3932338E-739.37540
ATGCCGT403.3932338E-739.37544
TCCGAGC504.7619324E-836.00000413
CTATTAT250.002092906336.0000041