##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_65.34100000006c2b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9008 Sequences flagged as poor quality 0 Sequence length 51 %GC 30 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.477353463587924 38.0 38.0 38.0 32.0 38.0 2 36.400310834813496 38.0 38.0 38.0 32.0 38.0 3 36.3735568383659 38.0 38.0 38.0 32.0 38.0 4 36.43916518650089 38.0 38.0 38.0 32.0 38.0 5 36.44660301953819 38.0 38.0 38.0 32.0 38.0 6 38.41762877442274 40.0 38.0 40.0 38.0 40.0 7 38.336256660746 40.0 38.0 40.0 38.0 40.0 8 38.308836589698046 40.0 38.0 40.0 38.0 40.0 9 38.25788188277087 40.0 38.0 40.0 38.0 40.0 10 38.183836589698046 40.0 38.0 40.0 38.0 40.0 11 38.18017317939609 40.0 38.0 40.0 38.0 40.0 12 38.02697602131439 40.0 38.0 40.0 32.0 40.0 13 38.008436944937834 40.0 38.0 40.0 32.0 40.0 14 37.93017317939609 40.0 38.0 40.0 32.0 40.0 15 37.88565719360568 40.0 38.0 40.0 32.0 40.0 16 37.88421403197158 40.0 38.0 40.0 32.0 40.0 17 37.959924511545296 40.0 38.0 40.0 32.0 40.0 18 38.05472912966252 40.0 38.0 40.0 32.0 40.0 19 38.06627442273535 40.0 38.0 40.0 32.0 40.0 20 38.13321492007105 40.0 38.0 40.0 38.0 40.0 21 38.15852575488455 40.0 38.0 40.0 38.0 40.0 22 38.152753108348136 40.0 38.0 40.0 32.0 40.0 23 38.14298401420959 40.0 38.0 40.0 38.0 40.0 24 38.11079040852575 40.0 38.0 40.0 38.0 40.0 25 38.046625222024865 40.0 38.0 40.0 32.0 40.0 26 38.00288632326821 40.0 38.0 40.0 32.0 40.0 27 38.01265541740675 40.0 38.0 40.0 32.0 40.0 28 37.95437388987567 40.0 38.0 40.0 32.0 40.0 29 37.949489342806395 40.0 38.0 40.0 32.0 40.0 30 37.94182948490231 40.0 38.0 40.0 32.0 40.0 31 37.917517761989345 40.0 38.0 40.0 32.0 40.0 32 37.84214031971581 40.0 38.0 40.0 32.0 40.0 33 37.796403197158085 40.0 38.0 40.0 32.0 40.0 34 37.82693161634103 40.0 38.0 40.0 32.0 40.0 35 37.85479573712256 40.0 38.0 40.0 32.0 40.0 36 37.800843694493786 40.0 38.0 40.0 32.0 40.0 37 37.753996447602134 40.0 38.0 40.0 32.0 40.0 38 37.69782415630551 40.0 38.0 40.0 32.0 40.0 39 37.71603019538188 40.0 38.0 40.0 32.0 40.0 40 37.67218028419183 40.0 38.0 40.0 32.0 40.0 41 37.59980017761989 40.0 38.0 40.0 32.0 40.0 42 37.588476909413856 40.0 38.0 40.0 32.0 40.0 43 37.49833481349911 40.0 38.0 40.0 32.0 40.0 44 37.39775754884547 40.0 38.0 40.0 32.0 40.0 45 37.38066163410302 40.0 38.0 40.0 32.0 40.0 46 37.27864120781528 40.0 38.0 40.0 32.0 40.0 47 37.27964031971581 40.0 38.0 40.0 32.0 40.0 48 37.15719360568384 40.0 38.0 40.0 32.0 40.0 49 36.99789076376554 40.0 38.0 40.0 32.0 40.0 50 36.930839253996446 40.0 38.0 40.0 32.0 40.0 51 36.791629662522205 40.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 1.162647666875607 2201 2 -0.23972129024978273 2201 3 -0.9862871286699288 2201 4 0.5999030687449718 2201 5 0.3914298056416641 2201 6 1.0469679702446228 2201 7 -1.7647871328018354 2201 8 0.6673805552596974 2201 9 0.5995466734552295 2201 10 0.16746997768368033 2201 11 0.46480640351274616 2201 12 1.023213186425501 2201 13 0.9008514004377801 2201 14 -0.6642797791465043 2201 15 -0.3321030136632288 2201 16 -0.5623641613186621 2201 17 -0.1720916708393716 2201 18 -1.4607605349103494 2201 19 -0.7877864004357633 2201 20 -0.44449407950096287 2201 21 -0.5125830779208655 2201 22 -1.7850234082196366 2201 23 -2.353885567557434 2201 24 -1.5547537506406925 2201 25 -1.98663155165913 2201 26 -2.279299950968152 2201 27 0.2051553621521549 2201 28 -1.1740408896658892 2201 29 -0.7552491842818227 2201 30 -1.2923495226689212 2201 31 -0.13513593101139776 2201 32 -1.2166378122490258 2201 33 -2.679812317737394 2201 34 0.7915342929129778 2201 35 -1.3903262995174686 2201 36 0.16708392455175414 2201 37 0.3098478612380191 2201 38 0.7857985598449773 2201 39 1.5928636505957314 2201 40 1.4962585884805577 2201 41 1.8673172479583329 2201 42 0.8251842392697242 2201 43 -0.17617975111080852 2201 44 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30 0.4576504773310788 1101 31 -0.7393025976780621 1101 32 0.5333621877509742 1101 33 0.9868543489292705 1101 34 1.2082009595796421 1101 35 1.4013403671491957 1101 36 -0.5620827421149102 1101 37 -0.6693188054286452 1101 38 -0.11003477348835844 1101 39 1.7595303172623957 1101 40 1.287925255147222 1101 41 1.3673172479583329 1101 42 1.8251842392697242 1101 43 1.1363202488891915 1101 44 1.2180691155760073 1101 45 -0.5137971849943312 1101 46 -1.6240938200382118 1101 47 -1.7406561244107266 1101 48 0.7343227442492264 1101 49 -0.6908554374547151 1101 50 1.3113871537469848 1101 51 0.38791078202842755 2202 1 0.7395707437986871 2202 2 0.29232999180149477 2202 3 0.6739692815864871 2202 4 1.138364607206512 2202 5 0.006814421026277273 2202 6 0.623891047167703 2202 7 0.2544436364289311 2202 8 -1.024927137047996 2202 9 -0.9389148650063106 2202 10 0.9110597212734248 2202 11 1.0160884547947973 2202 12 1.0873157505280702 2202 13 0.9777744773608603 2202 14 -0.74761311247984 2202 15 1.1614867299265157 2202 16 1.2645589156044181 2202 17 -0.9220916708393716 2202 18 0.4174445932947748 2202 19 0.5647777021283389 2202 20 0.40807002306313933 2202 21 -0.10873692407471225 2202 22 0.18933556613933433 2202 23 0.12047340680153695 2202 24 1.2465283006413586 2202 25 1.2441376791101035 2202 26 0.13095645928826372 2202 27 1.032078439075235 2202 28 0.8451898795648773 2202 29 -1.620633799666436 2202 30 0.7268812465618453 2202 31 -0.20564875152421536 2202 32 -1.4281762737874857 2202 33 -0.5516071895322696 2202 34 0.5928163441950289 2202 35 0.9494172902261155 2202 36 0.865801873269703 2202 37 0.758565809955968 2202 38 1.1255421495885614 2202 39 -0.35585429812221747 2202 40 1.2398483320703022 2202 41 0.13654801718909937 2202 42 1.4021073161928044 2202 43 1.6507433258122717 2202 44 1.6074921924990875 2202 45 -0.5907202619174114 2202 46 0.611483103038708 2202 47 1.4324207986661932 2202 48 1.6573996673261462 2202 49 -0.527393898993175 2202 50 1.5854256152854447 2202 51 1.5369492435668874 1102 1 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49 0.9929680919570529 1212 50 0.12388715374698478 1212 51 -0.3363539238539275 1213 1 -1.024852333124393 1213 2 -1.1980546235831184 1213 3 0.8470462046634069 1213 4 -0.3375969312550282 1213 5 -0.9210701943583359 1213 6 -0.07803202975537715 1213 7 0.11021286719816459 1213 8 -1.6451194447403026 1213 9 0.0995466734552295 1213 10 -1.3533633556496554 1213 11 -1.0560269298205895 1213 12 0.10654651975883667 1213 13 -3.22414859956222 1213 14 -0.56011311247984 1213 15 -0.6029363469965645 1213 16 -0.18736416131866207 1213 17 -0.4845916708393716 1213 18 -1.669093868243685 1213 19 -1.7044530671024276 1213 20 -0.9236607461676272 1213 21 -2.3875830779208655 1213 22 -2.2433567415529723 1213 23 -0.6247189008907696 1213 24 0.17441291602597175 1213 25 0.63836844834087 1213 26 1.1790333823651835 1213 27 0.2676553621521549 1213 28 -0.48654088966588915 1213 29 0.49475081571817725 1213 30 -0.7298495226689212 1213 31 -0.7393025976780621 1213 32 0.9083621877509742 1213 33 0.1743543489292705 1213 34 -0.6667990404203579 1213 35 -0.03615963285080426 1213 36 -0.31208274211491016 1213 37 0.8306811945713548 1213 38 0.45246522651164156 1213 39 0.3845303172623957 1213 40 0.787925255147222 1213 41 0.9923172479583329 1213 42 0.5751842392697242 1213 43 1.2613202488891915 1213 44 -0.9069308844239927 1213 45 0.048702815005668754 1213 46 -1.3115938200382118 1213 47 -0.05315612441072659 1213 48 -2.0156772557507736 1213 49 -0.19085543745471512 1213 50 0.5613871537469848 1213 51 1.0129107820284275 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 1.0 19 4.0 20 0.0 21 7.0 22 10.0 23 18.0 24 17.0 25 23.0 26 42.0 27 62.0 28 77.0 29 98.0 30 107.0 31 141.0 32 195.0 33 249.0 34 311.0 35 345.0 36 525.0 37 769.0 38 1364.0 39 4642.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.26271374639129 27.093049078392184 13.879635798356652 19.764601376859872 2 11.645204262877442 28.97424511545293 36.37877442273535 23.00177619893428 3 20.503996447602134 32.793072824156305 29.49600355239787 17.206927175843695 4 11.956039076376554 26.709591474245116 33.259325044404974 28.075044404973358 5 11.101243339253998 38.399200710479576 32.981793960923625 17.517761989342805 6 26.798401420959145 42.77309058614564 13.265985790408527 17.16252220248668 7 16.407637655417407 42.47335701598579 19.08303730017762 22.035968028419184 8 20.426287744227352 38.14387211367673 21.114564831261102 20.315275310834814 9 20.30417406749556 26.709591474245116 21.24777975133215 31.73845470692718 10 14.36500888099467 35.31305506216697 26.60968028419183 23.712255772646536 11 23.878774422735347 29.718028419182946 22.924067495559502 23.4791296625222 12 20.093250444049733 34.52486678507993 27.364564831261102 18.01731793960924 13 23.734458259325045 27.364564831261102 28.69671403197158 20.20426287744227 14 18.22824156305506 29.018650088809945 30.561722912966253 22.19138543516874 15 21.55861456483126 31.849467140319714 27.675399644760212 18.91651865008881 16 15.763765541740673 37.02264653641208 27.031527531083483 20.182060390763766 17 14.065275310834814 37.5 31.16119005328597 17.273534635879216 18 15.452930728241562 30.839253996447603 37.97735346358792 15.730461811722913 19 14.775754884547068 34.535968028419184 35.02442273534636 15.66385435168739 20 14.065275310834814 31.283303730017764 36.00133214920071 18.650088809946713 21 11.201154529307281 36.13454706927176 35.52397868561279 17.140319715808168 22 11.922735346358792 39.15408525754884 35.84591474245116 13.077264653641208 23 12.122557726465365 35.34635879218472 36.22335701598579 16.30772646536412 24 15.264209591474245 36.37877442273535 36.26776198934281 12.089253996447603 25 13.44360568383659 38.16607460035524 35.5461811722913 12.844138543516873 26 13.909857904085257 37.07815275310835 36.37877442273535 12.633214920071048 27 12.533303730017762 38.85435168738899 36.789520426287744 11.822824156305506 28 11.734014209591475 36.54529307282416 36.73401420959148 14.986678507992895 29 14.797957371225579 36.98934280639431 36.511989342806395 11.700710479573711 30 11.789520426287744 39.56483126110124 35.65719360568384 12.988454706927177 31 12.233570159857903 37.16696269982238 35.80150976909414 14.797957371225579 32 14.120781527531085 37.28907637655418 35.61278863232682 12.97735346358792 33 11.034635879218472 39.043072824156305 36.045737122557725 13.876554174067495 34 11.190053285968029 39.209591474245116 37.87744227353463 11.72291296625222 35 11.767317939609235 40.98579040852576 36.00133214920071 11.245559502664298 36 11.412078152753107 38.898756660746 37.82193605683837 11.867229129662523 37 11.35657193605684 41.13010657193606 35.65719360568384 11.856127886323268 38 11.33436944937833 38.254884547069274 38.210479573712256 12.200266429840143 39 11.101243339253998 38.3436944937833 36.12344582593251 14.431616341030196 40 11.545293072824157 37.77753108348135 38.49911190053286 12.178063943161634 41 11.9449378330373 38.26598579040852 35.401865008880996 14.387211367673181 42 13.399200710479573 38.232682060390765 35.80150976909414 12.566607460035522 43 11.178952042628774 39.1651865008881 37.83303730017762 11.822824156305506 44 11.134547069271758 40.87477797513321 36.023534635879216 11.967140319715808 45 12.122557726465365 38.96536412078152 37.66651865008881 11.245559502664298 46 14.198490230905861 37.855239786856124 34.957815275310836 12.988454706927177 47 10.72380106571936 38.62122557726465 34.69138543516873 15.963587921847248 48 11.289964476021314 38.62122557726465 34.66918294849023 15.419626998223801 49 13.576820603907638 38.31039076376554 35.1576376554174 12.955150976909414 50 10.934724689165186 38.321492007104794 37.01154529307282 13.732238010657195 51 11.212255772646536 37.744227353463586 35.11323268206039 15.930284191829486 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 660.0 1 363.0 2 66.0 3 83.0 4 100.0 5 104.0 6 108.0 7 122.0 8 136.0 9 121.0 10 106.0 11 205.0 12 304.0 13 469.5 14 635.0 15 675.5 16 716.0 17 535.5 18 355.0 19 274.5 20 194.0 21 282.0 22 370.0 23 254.0 24 138.0 25 148.5 26 218.5 27 278.0 28 192.5 29 107.0 30 118.0 31 129.0 32 157.5 33 186.0 34 209.5 35 233.0 36 264.0 37 295.0 38 298.0 39 301.0 40 301.5 41 302.0 42 303.0 43 304.0 44 329.0 45 354.0 46 344.5 47 335.0 48 337.5 49 340.0 50 353.0 51 366.0 52 448.0 53 530.0 54 390.5 55 251.0 56 222.0 57 193.0 58 164.0 59 135.0 60 123.0 61 111.0 62 92.5 63 74.0 64 66.0 65 58.0 66 50.0 67 42.0 68 30.0 69 18.0 70 13.5 71 9.0 72 7.0 73 5.0 74 3.5 75 2.0 76 2.0 77 1.5 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022202486678507993 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 9008.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.04840142095915 #Duplication Level Percentage of deduplicated Percentage of total 1 95.85006693440428 55.63943161634103 2 1.8167909734174794 2.109236234458259 3 0.4016064257028112 0.6993783303730018 4 0.24861350162554982 0.5772646536412078 5 0.09562057754828839 0.2775310834813499 6 0.21036527060623444 0.7326820603907638 7 0.11474469305794606 0.4662522202486678 8 0.07649646203863071 0.3552397868561279 9 0.05737234652897303 0.2997335701598579 >10 0.8797093134442533 11.489786856127886 >50 0.09562057754828839 4.0630550621669625 >100 0.13386880856760375 16.207815275310836 >500 0.019124115509657678 7.08259325044405 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 638 7.08259325044405 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 384 4.262877442273535 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 228 2.5310834813499112 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 219 2.431172291296625 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 203 2.253552397868561 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 196 2.1758436944937833 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 125 1.3876554174067497 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 105 1.1656305506216695 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 92 1.0213143872113677 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 76 0.8436944937833037 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 73 0.8103907637655416 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 71 0.7881882770870338 No Hit GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 54 0.5994671403197158 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 49 0.5439609236234458 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 47 0.5217584369449378 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 44 0.4884547069271759 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43 0.47735346358792186 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43 0.47735346358792186 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41 0.45515097690941386 No Hit TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 40 0.4440497335701598 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39 0.43294849023090587 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA 38 0.4218472468916519 No Hit GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 36 0.3996447602131439 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 36 0.3996447602131439 No Hit GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 34 0.3774422735346359 No Hit GATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 33 0.3663410301953819 No Hit TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT 32 0.3552397868561279 Illumina PCR Primer Index 11 (95% over 21bp) GCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23 0.2553285968028419 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23 0.2553285968028419 No Hit CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21 0.2331261101243339 No Hit ATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 19 0.21092362344582594 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 19 0.21092362344582594 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAA 19 0.21092362344582594 No Hit TGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18 0.19982238010657194 No Hit ATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18 0.19982238010657194 No Hit TACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 17 0.18872113676731794 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17 0.18872113676731794 No Hit ACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.17761989342806395 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.17761989342806395 No Hit TGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.17761989342806395 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAA 16 0.17761989342806395 No Hit GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15 0.16651865008880995 No Hit ATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15 0.16651865008880995 No Hit CCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15 0.16651865008880995 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.15541740674955595 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.15541740674955595 No Hit CTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13 0.14431616341030196 No Hit GATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13 0.14431616341030196 No Hit TTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12 0.13321492007104796 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAA 12 0.13321492007104796 No Hit GCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12 0.13321492007104796 No Hit GATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12 0.13321492007104796 No Hit GTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12 0.13321492007104796 No Hit GTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.12211367673179398 No Hit TGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.12211367673179398 No Hit ACTCTGCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.12211367673179398 No Hit CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.11101243339253995 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAA 10 0.11101243339253995 No Hit GTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10 0.11101243339253995 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.022202486678507993 20 0.0 0.0 0.022202486678507993 21 0.0 0.0 0.022202486678507993 22 0.0 0.0 0.03330373001776199 23 0.0 0.0 0.08880994671403197 24 0.0 0.0 0.08880994671403197 25 0.0 0.0 0.08880994671403197 26 0.0 0.0 0.09991119005328597 27 0.0 0.0 0.12211367673179396 28 0.0 0.0 0.19982238010657194 29 0.0 0.0 0.2775310834813499 30 0.0 0.0 0.3441385435168739 31 0.0 0.0 0.5106571936056838 32 0.0 0.0 0.6549733570159858 33 0.0 0.0 0.8992007104795737 34 0.0 0.0 1.1323268206039077 35 0.0 0.0 1.3210479573712255 36 0.0 0.0 1.6984902309058614 37 0.0 0.0 2.064831261101243 38 0.0 0.0 2.4977797513321494 39 0.0 0.0 2.886323268206039 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 80 8.330426E-8 25.3125 1 TATCAAC 110 1.7948405E-6 18.40909 2 ATCAACG 120 4.12438E-6 16.875 3 AACGCAG 125 6.086544E-6 16.199999 6 TCAACGC 125 6.086544E-6 16.199999 4 CAACGCA 125 6.086544E-6 16.199999 5 ACGCAGA 140 1.7853656E-5 14.464285 7 CGCAGAG 140 1.7853656E-5 14.464285 8 AGAGTAC 140 3.0278906E-4 12.857142 11 GCAGAGT 140 3.0278906E-4 12.857142 9 CAGAGTA 145 4.0744504E-4 12.413794 10 ACTTTTT 155 7.149629E-4 11.612903 16 CTTTTTT 165 0.0012083647 10.909092 17 GTACTTT 145 0.005978642 10.86207 14 AGTACTT 145 0.005978642 10.86207 13 GAGTACT 145 0.005978642 10.86207 12 TACTTTT 145 0.005978642 10.86207 15 >>END_MODULE