H23NVBCXX l02n01 48h_64.34100000006c1e.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 48h_64.34100000006c1e.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38907
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC	3603	9.260544375048193	No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT	366	0.9407047574986507	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	191	0.49091423137224666	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCAAGCCTGTCTCTTATACACA	177	0.4549309892821343	No Hit
GCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA	171	0.43950959981494336	No Hit
GCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA	169	0.434369136659213	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	151	0.38810496825764	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGAAGCCTGTCTCTTATACACA	146	0.3752538103683142	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC	130	0.33413010512247154	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC	93	0.2390315367414604	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC	92	0.23646130516359523	No Hit
GCTTCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC	91	0.23389107358573008	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCAAGCCTGTCTCTTATACACATC	87	0.22361014727426942	No Hit
GCTTGAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC	83	0.21332922096280876	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGAAGCCTGTCTCTTATACACATC	82	0.21075898938494358	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70	0.1799162104505616	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA	68	0.17477574729483125	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61	0.1567841262497751	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT	59	0.15164366309404478	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCAAGCAGTGGTATCACTGTCT	49	0.1259413473153931	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT	46	0.11823065258179762	No Hit
GCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATAC	45	0.11566042100393245	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44	0.11309018942606729	No Hit
GCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATAC	43	0.11051995784820212	No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTGAAGCCTGTCTCTTATAC	39	0.10023903153674146	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	510	0.0	39.264706	45
ATGCCGT	515	0.0	38.883495	44
TATGCCG	520	0.0	38.509617	43
GGCGAAT	485	0.0	38.505157	32
ATCTCGT	515	0.0	38.4466	37
TCTCGTA	515	0.0	38.4466	38
CGTATGC	515	0.0	38.4466	41
TCGTATG	515	0.0	38.4466	40
CTCGTAT	515	0.0	38.4466	39
CGAATCT	495	0.0	38.181816	34
GAATCTC	495	0.0	38.181816	35
GCGAATC	490	0.0	38.112244	33
AGGCGAA	490	0.0	38.112244	31
AAGGCGA	490	0.0	38.112244	30
GTATGCC	520	0.0	38.076927	42
GCCGTCT	65	7.2759576E-12	38.076927	45
AATCTCG	510	0.0	37.941177	36
CCAAGGC	500	0.0	37.8	28
CAAGGCG	495	0.0	37.727272	29
CGAGACC	505	0.0	37.425743	23

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content