##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H23NVBCXX l02n01 48h_62.34100000006bf3.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10234
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.87805354700019	38.0	32.0	38.0	32.0	38.0
2	36.11461794019934	38.0	38.0	38.0	32.0	38.0
3	36.009478209888606	38.0	32.0	38.0	32.0	38.0
4	36.247312878639825	38.0	38.0	38.0	32.0	38.0
5	36.21115888215751	38.0	38.0	38.0	32.0	38.0
6	38.19493844049248	40.0	38.0	40.0	38.0	40.0
7	37.82323627125269	40.0	38.0	40.0	32.0	40.0
8	37.787473128786395	40.0	38.0	40.0	32.0	40.0
9	38.046316200898964	40.0	38.0	40.0	32.0	40.0
10	37.88205980066445	40.0	38.0	40.0	32.0	40.0
11	37.990521790111394	40.0	38.0	40.0	32.0	40.0
12	37.611784248583156	40.0	38.0	40.0	32.0	40.0
13	37.882255227672466	40.0	38.0	40.0	32.0	40.0
14	37.59175298026187	40.0	38.0	40.0	32.0	40.0
15	37.669044361930816	40.0	38.0	40.0	32.0	40.0
16	37.254445964432286	38.0	38.0	40.0	32.0	40.0
17	37.20119210474888	38.0	38.0	40.0	32.0	40.0
18	37.03742427203439	38.0	38.0	40.0	32.0	40.0
19	37.30486613249951	40.0	38.0	40.0	32.0	40.0
20	37.419093218682825	40.0	38.0	40.0	32.0	40.0
21	37.63142466288841	40.0	38.0	40.0	32.0	40.0
22	37.72698846980653	40.0	38.0	40.0	32.0	40.0
23	37.713797146765685	40.0	38.0	40.0	32.0	40.0
24	37.52902091068986	40.0	38.0	40.0	32.0	40.0
25	37.506155950752394	40.0	38.0	40.0	32.0	40.0
26	37.279949188977916	40.0	38.0	40.0	32.0	40.0
27	37.32978307602111	40.0	38.0	40.0	32.0	40.0
28	37.40981043580223	40.0	38.0	40.0	32.0	40.0
29	37.53009575923392	40.0	38.0	40.0	32.0	40.0
30	37.54944303302717	40.0	38.0	40.0	32.0	40.0
31	37.507914793824504	40.0	38.0	40.0	32.0	40.0
32	37.34043384795779	40.0	38.0	40.0	32.0	40.0
33	37.05061559507524	38.0	38.0	40.0	32.0	40.0
34	37.36202853234317	40.0	38.0	40.0	32.0	40.0
35	37.20949775258941	40.0	38.0	40.0	32.0	40.0
36	36.95954660934141	38.0	38.0	40.0	32.0	40.0
37	37.07279656048466	38.0	38.0	40.0	32.0	40.0
38	37.195817862028534	38.0	38.0	40.0	32.0	40.0
39	36.999609145983975	38.0	38.0	40.0	32.0	40.0
40	37.031952315810045	38.0	38.0	40.0	32.0	40.0
41	37.15604846589799	40.0	38.0	40.0	32.0	40.0
42	37.222884502638266	38.0	38.0	40.0	32.0	40.0
43	37.51622044166504	40.0	38.0	40.0	32.0	40.0
44	37.21203830369357	40.0	38.0	40.0	32.0	40.0
45	37.40834473324213	40.0	38.0	40.0	32.0	40.0
46	36.97205393785421	38.0	38.0	40.0	32.0	40.0
47	37.04651162790697	38.0	38.0	40.0	32.0	40.0
48	37.00537424272034	38.0	38.0	40.0	32.0	40.0
49	36.82724252491694	38.0	38.0	40.0	32.0	40.0
50	36.586965018565564	38.0	38.0	40.0	32.0	40.0
51	36.190248192300174	38.0	38.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
2201	1	0.54854843379659
2201	2	0.6920465971265344
2201	3	1.5039030361005672
2201	4	1.4013972112124193
2201	5	1.1180046736137115
2201	6	1.3300645399438409
2201	7	0.02615049994778218
2201	8	0.7422456241057489
2201	9	0.5895395296487393
2201	10	0.9994734077841514
2201	11	0.9448973923661868
2201	12	1.880740582543737
2201	13	1.5607433665942807
2201	14	2.19759134591758
2201	15	0.9283641929697026
2201	16	0.9756683020711776
2201	17	0.7704544682667276
2201	18	0.9689113801306632
2201	19	1.585401237291478
2201	20	0.02598092743760816
2201	21	0.9965123646455893
2201	22	0.8587427086640957
2201	23	1.0443070928818514
2201	24	1.107836845306629
2201	25	1.25772417129388
2201	26	2.045063361144905
2201	27	0.9853413810910752
2201	28	1.5689137597304494
2201	29	1.6463678469658731
2201	30	0.9620686338595732
2201	31	1.1768674601461697
2201	32	1.0132708013198624
2201	33	1.5620071186580091
2201	34	1.2995329420778248
2201	35	1.6935298371836893
2201	36	0.04153698253931282
2201	37	1.0371564678924798
2201	38	1.2726012216627751
2201	39	2.0875400567193765
2201	40	1.978433750672174
2201	41	1.2421114916108067
2201	42	2.276218566985669
2201	43	1.8870558587385773
2201	44	1.7936072402560796
2201	45	0.5030961745781397
2201	46	1.9631063601216425
2201	47	1.583458211747086
2201	48	2.1842566763758526
2201	49	1.819643985293169
2201	50	2.091554111523152
2201	51	0.17112914497784004
1101	1	1.191405576653736
1101	2	0.9599037399836803
1101	3	-0.1925255353280022
1101	4	0.062111496926704035
1101	5	1.8680046736137115
1101	6	0.8300645399438409
1101	7	1.1957933570906363
1101	8	-0.4809686616085358
1101	9	-0.41046047035126065
1101	10	1.410187693498436
1101	11	-0.41224546477666735
1101	12	1.523597725400883
1101	13	1.4714576523085654
1101	14	1.0011627744890106
1101	15	1.2765784786839873
1101	16	1.8060254449283235
1101	17	1.8150973254095817
1101	18	0.7814113801306632
1101	19	-0.4413844769942372
1101	20	1.7134809274376082
1101	21	0.22865522178844344
1101	22	-0.40911443419305016
1101	23	0.7139499500247055
1101	24	1.3221225595923443
1101	25	1.3648670284367341
1101	26	1.545063361144905
1101	27	2.4317699525196446
1101	28	1.3903423311590188
1101	29	1.1106535612515884
1101	30	1.997782919573858
1101	31	2.516153174431885
1101	32	2.066842229891293
1101	33	2.347721404372294
1101	34	2.0852472277921095
1101	35	1.9435298371836893
1101	36	2.086179839682167
1101	37	2.28715646789248
1101	38	0.8976012216627751
1101	39	1.9268257710050918
1101	40	2.228433750672174
1101	41	0.1260400630393761
1101	42	1.6333614241285233
1101	43	0.3691987158814314
1101	44	2.0793215259703643
1101	45	1.7084533174352856
1101	46	2.641677788693073
1101	47	2.6995296403185165
1101	48	2.0413995335187067
1101	49	0.757143985293169
1101	50	0.8236969686660061
1101	51	2.403272002120694
2202	1	-1.1822207969726435
2202	2	-1.0291072490273123
2202	3	-1.8903277331301993
2202	4	0.39178182659703253
2202	5	0.021850827459864774
2202	6	0.7531414630207607
2202	7	0.3386504999477822
2202	8	0.4585917779519022
2202	9	-1.064306624197414
2202	10	0.12447340778415139
2202	11	-1.2089487614799665
2202	12	0.4576636594668173
2202	13	0.9549741358250472
2202	14	1.3418221151483465
2202	15	0.6447103468158559
2202	16	-1.8752932363903625
2202	17	-2.1958916855794257
2202	18	-5.180127081407797
2202	19	-2.5347910704008285
2202	20	-0.13267291871623854
2202	21	1.6022815954148228
2202	22	0.5414350163564023
2202	23	-0.4893467532719953
2202	24	0.30014453761432236
2202	25	-2.03073736716766
2202	26	0.545063361144905
2202	27	0.7064952272449219
2202	28	-1.2580093171926308
2202	29	-0.14209369149566697
2202	30	0.1736070953980331
2202	31	0.5710982293769362
2202	32	-1.2848061217570574
2202	33	-0.09183903518814418
2202	34	-0.6620055194606351
2202	35	-0.6718547782009239
2202	36	-0.8478860943837674
2202	37	-1.0205358397998268
2202	38	0.05144737550892842
2202	39	1.2894631336424567
2202	40	0.3822799045183274
2202	41	0.4392268762261935
2202	42	0.31468010544720926
2202	43	0.9735943202770372
2202	44	-1.7448542982054605
2202	45	0.719442328424293
2202	46	-1.6330474860322042
2202	47	-3.190580249791374
2202	48	-0.9695894774703007
2202	49	-2.4351637070145244
2202	50	-1.6872920423230013
2202	51	-2.41540931656062
1102	1	0.695607257326003
1102	2	-1.3006004616969946
1102	3	0.11419715374762518
1102	4	-2.1647792593758126
1102	5	-2.1319953263862885
1102	6	-0.6405236953502751
1102	7	-1.6613495000522178
1102	8	0.06945150645869091
1102	9	-1.3222251762336157
1102	10	-0.3461148275099646
1102	11	-0.8786320193985162
1102	12	-0.7369064762797919
1102	13	-1.3436683981116033
1102	14	-0.25093806584712297
1102	15	-0.2738416893832394
1102	16	-0.9838905214582354
1102	17	1.4873662329726116
1102	18	0.16376432130713425
1102	19	-0.22709876270852192
1102	20	0.8899515156729052
1102	21	-0.9226052824132367
1102	22	0.8219780027817407
1102	23	1.2979835634700834
1102	24	-0.03922197822278406
1102	25	0.743018288940938
1102	26	0.13329865526254991
1102	27	1.2721060869734302
1102	28	-0.5928509461519056
1102	29	-2.191867447151772
1102	30	-0.5820490131992528
1102	31	-0.08048548103030129
1102	32	0.3441531542610363
1102	33	-0.11446346957728792
1102	34	-1.0828199990986462
1102	35	-1.5270583981104267
1102	36	-0.3003747821665712
1102	37	-1.1834317674016361
1102	38	-1.5729870136313409
1102	39	0.5654812331899635
1102	40	1.816669044789819
1102	41	-2.7689179201538963
1102	42	-3.694369668308447
1102	43	0.8061735057974033
1102	44	-1.7946280538615653
1102	45	-0.7285214724806863
1102	46	-1.0221877575254155
1102	47	-2.787865317664682
1102	48	-1.4039786177417923
1102	49	-0.978150132353889
1102	50	0.584201170346681
1102	51	-0.30681203149274694
2203	1	0.15381159169132985
2203	2	0.6065202813370618
2203	3	1.2473240887321495
2203	4	0.2500814217387344
2203	5	1.2890573051926566
2203	6	0.724801382049101
2203	7	0.0754926052109397
2203	8	-0.3268333232626688
2203	9	0.8263816349118969
2203	10	0.07184182883678147
2203	11	0.7870026555240841
2203	12	1.0649511088595247
2203	13	-0.6563618965636167
2203	14	0.6778545038123198
2203	15	-0.2459779122934549
2203	16	1.8210630389132803
2203	17	-0.8315192159437998
2203	18	-0.08700967250091907
2203	19	0.7202696583441082
2203	20	0.1871651379639232
2203	21	0.0030913120140070305
2203	22	-1.5557309755464317
2203	23	-0.6431929071181486
2203	24	-0.6026894704828436
2203	25	-0.5975389866008598
2203	26	1.2819054664080625
2203	27	0.12349927582791764
2203	28	0.23996639130939457
2203	29	0.2911046890711333
2203	30	0.7647002128069431
2203	31	0.0650253548830122
2203	32	0.1119550118461774
2203	33	-0.9906244602893608
2203	34	-0.6741512684484903
2203	35	0.575108784552107
2203	36	0.9658790878024703
2203	37	0.33978804683984976
2203	38	-0.5760829888635399
2203	39	1.1599084777720066
2203	40	2.281065329619544
2203	41	1.2125062284529093
2203	42	1.355165935406724
2203	43	0.14363480610699497
2203	44	1.162028292887662
2203	45	1.1971751219465574
2203	46	-0.1553146925099398
2203	47	-0.7290417882529141
2203	48	-0.3420591130978323
2203	49	0.20451240634579904
2203	50	-0.7966037832136905
2203	51	-1.2696603287063724
1103	1	-0.13895156620340998
1103	2	-0.8079534028734656
1103	3	0.003903036100567192
1103	4	-0.09860278878758066
1103	5	-0.006995326386288525
1103	6	0.8300645399438409
1103	7	0.3386504999477822
1103	8	0.6797456241057489
1103	9	-0.8479604703512607
1103	10	0.12447340778415139
1103	11	-0.05510260763381325
1103	12	0.6307405825437371
1103	13	0.3107433665942807
1103	14	1.32259134591758
1103	15	1.1158641929697026
1103	16	1.0381683020711776
1103	17	1.3329544682667276
1103	18	-0.8435886198693368
1103	19	0.08540123729147808
1103	20	0.33848092743760816
1103	21	1.2465123646455893
1103	22	-0.8287572913359043
1103	23	0.8568070928818514
1103	24	0.982836845306629
1103	25	0.38272417129388003
1103	26	-1.517436638855095
1103	27	-2.014658618908925
1103	28	0.006413759730449442
1103	29	0.20886784696587313
1103	30	0.9620686338595732
1103	31	-0.01063253985383028
1103	32	-0.29922919868013764
1103	33	0.9370071186580091
1103	34	1.6745329420778248
1103	35	1.3185298371836893
1103	36	0.9165369825393128
1103	37	0.34965646789247984
1103	38	1.5226012216627751
1103	39	2.0875400567193765
1103	40	1.978433750672174
1103	41	0.3671114916108067
1103	42	0.7762185669856692
1103	43	-1.4254441412614227
1103	44	-0.6438927597439204
1103	45	1.4405961745781397
1103	46	1.8381063601216425
1103	47	1.395958211747086
1103	48	1.1842566763758526
1103	49	0.44464398529316895
1103	50	2.216554111523152
1103	51	0.42112914497784004
2204	1	0.54854843379659
2204	2	-0.4329534028734656
2204	3	0.003903036100567192
2204	4	-0.9111027887875807
2204	5	0.7430046736137115
2204	6	0.4550645399438409
2204	7	-0.7238495000522178
2204	8	-2.195254375894251
2204	9	0.7770395296487393
2204	10	0.4994734077841514
2204	11	-0.36760260763381325
2204	12	0.3807405825437371
2204	13	0.4357433665942807
2204	14	0.32259134591758
2204	15	-0.25913580703029737
2204	16	-0.8993316979288224
2204	17	-2.8545455317332724
2204	18	-2.1560886198693368
2204	19	-1.914598762708522
2204	20	0.21348092743760816
2204	21	0.24651236464558934
2204	22	-1.3287572913359043
2204	23	-0.8306929071181486
2204	24	0.982836845306629
2204	25	-0.80477582870612
2204	26	-1.704936638855095
2204	27	-1.6396586189089248
2204	28	0.5064137597304494
2204	29	-0.6036321530341269
2204	30	-0.8504313661404268
2204	31	0.4893674601461697
2204	32	0.38827080131986236
2204	33	0.24950711865800912
2204	34	1.2995329420778248
2204	35	0.5685298371836893
2204	36	-0.08346301746068718
2204	37	-0.27534353210752016
2204	38	-1.6648987783372249
2204	39	0.02504005671937648
2204	40	0.6659337506721741
2204	41	-1.0703885083891933
2204	42	0.46371856698566916
2204	43	0.8245558587385773
2204	44	0.7311072402560796
2204	45	0.9405961745781397
2204	46	-0.5993936398783575
2204	47	-1.229041788252914
2204	48	-0.6282433236241474
2204	49	0.694643985293169
2204	50	1.529054111523152
2204	51	1.48362914497784
2205	1	-0.45145156620341
2205	2	-0.4954534028734656
2205	3	-0.3710969638994328
2205	4	-0.47360278878758066
2205	5	0.055504673613711475
2205	6	-1.9824354600561591
2205	7	-1.5988495000522178
2205	8	-0.9452543758942511
2205	9	-3.0354604703512607
2205	10	-0.8755265922158486
2205	11	-0.18010260763381325
2205	12	-2.994259417456263
2205	13	-0.5642566334057193
2205	14	-1.67740865408242
2205	15	-0.5091358070302974
2205	16	-2.9618316979288224
2205	17	-2.8545455317332724
2205	18	-3.2185886198693368
2205	19	-4.539598762708522
2205	20	-3.286519072562392
2205	21	-0.8159876353544107
2205	22	-0.14125729133590426
2205	23	-0.8306929071181486
2205	24	-0.20466315469337104
2205	25	-0.74227582870612
2205	26	-0.579936638855095
2205	27	-2.577158618908925
2205	28	-2.3060862402695506
2205	29	-2.166132153034127
2205	30	-0.0379313661404268
2205	31	0.23936746014616972
2205	32	-0.36172919868013764
2205	33	0.6245071186580091
2205	34	0.29953294207782477
2205	35	0.19352983718368932
2205	36	-0.7709630174606872
2205	37	0.41215646789247984
2205	38	-1.1648987783372249
2205	39	-1.9749599432806235
2205	40	-2.459066249327826
2205	41	-1.7578885083891933
2205	42	-4.161281433014331
2205	43	-0.9879441412614227
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1211	3	-0.5099858527883185
1211	4	-0.6124916776764664
1211	5	-0.35421754860850996
1211	6	0.7189534288327266
1211	7	0.17198383328111788
1211	8	-1.0841432647831368
1211	9	0.03398397409318221
1211	10	0.18002896333970853
1211	11	4.5294792174388476E-4
1211	12	-1.0081483063451486
1211	13	-0.647589966739055
1211	14	-0.6496308763046414
1211	15	-0.6202469181414116
1211	16	-0.44794280903993666
1211	17	-0.09760108728882955
1211	18	2.059189157908442
1211	19	0.05067901506925665
1211	20	-0.2309635170068347
1211	21	0.20484569797892505
1211	22	1.2337427086640957
1211	23	0.9123626484374086
1211	24	1.2745035119732933
1211	25	1.1743908379605443
1211	26	0.7117300278115692
1211	27	0.2492302699799609
1211	28	1.0411359819526709
1211	29	1.1185900691880946
1211	30	-0.06570914391820537
1211	31	-0.30924365096494455
1211	32	-0.19506253201347334
1211	33	0.8953404519913448
1211	34	0.29953294207782477
1211	35	0.11019650385035362
1211	36	-1.7709630174606872
1211	37	-1.8795101987741845
1211	38	-0.6023987783372249
1211	39	-0.4541266099472878
1211	40	1.672878195116617
1211	41	0.5546114916108067
1211	42	-0.8904480996809951
1211	43	1.400944747627463
1211	44	-1.039726093077256
1211	45	-0.04551493653297456
1211	46	0.10199524901052825
1211	47	1.2154026561915288
1211	48	0.35092334304251693
1211	49	0.9793662075153904
1211	50	0.4109985559675948
1211	51	0.9350180338667258
1208	1	1.3818817671299257
1208	2	0.8170465971265344
1208	3	0.8789030361005672
1208	4	-0.5013805665653592
1208	5	0.5902268958359329
1208	6	-0.058824348945044846
1208	7	-1.3280161667188821
1208	8	-1.2508099314498082
1208	9	-0.13268269257348209
1208	10	-0.59774881443807
1208	11	0.16711961458840818
1208	12	0.2140739158770728
1208	13	0.6857433665942807
1208	14	-0.03851976519353428
1208	15	-1.1758024736969617
1208	16	-1.9479428090399367
1208	17	0.5135100238222847
1208	18	-0.5519219532026725
1208	19	0.1617901261803638
1208	20	-1.1754079614512776
1208	21	-1.9618209686877464
1208	22	-0.15514618022479
1208	23	-0.42097068489592715
1208	24	-0.7254964880267067
1208	25	1.7299463935161015
1208	26	-0.8993810832995379
1208	27	1.749230269979961
1208	28	-0.18108624026955056
1208	29	-0.8258543752563483
1208	30	0.7676241894151303
1208	31	1.5796452379239483
1208	32	1.0827152457643052
1208	33	0.11756267421356625
1208	34	1.355088497633382
1208	35	2.1657520594059108
1208	36	0.3957036492059771
1208	37	1.398267579003594
1208	38	0.6753789994405537
1208	39	-0.17634883216950925
1208	40	1.3951004173388384
1208	41	0.7768337138330281
1208	42	0.9428852336523335
1208	43	1.400944747627463
1208	44	-1.2063927597439204
1208	45	-0.4899593809774174
1208	46	-1.2868936398783575
1208	47	-0.8957084549195784
1208	48	0.18425667637585263
1208	49	0.8682550964042832
1208	50	0.5221096670787091
1208	51	2.490573589422283
1209	1	1.191405576653736
1209	2	-1.6115248314448962
1209	3	0.5931887503862825
1209	4	-0.7236027887875807
1209	5	-0.7034238978148579
1209	6	0.687207397086695
1209	7	-0.08992092862364842
1209	8	1.161888481248603
1209	9	0.23239667250588525
1209	10	0.05304483635558199
1209	11	0.659183106651902
1209	12	0.8093120111151677
1209	13	-0.7428280619771499
1209	14	-1.2131229397967047
1209	15	0.3480070501125567
1209	16	1.377454016356893
1209	17	0.3150973254095817
1209	18	-0.07573147701219085
1209	19	1.2014726658629087
1209	20	0.7849094988661776
1209	21	1.2286552217884434
1209	22	-0.6948287199073349
1209	23	1.3568070928818514
1209	24	-0.46359172612194044
1209	25	1.0791527427224494
1209	26	1.545063361144905
1209	27	2.003198523948221
1209	28	1.5331994740161647
1209	29	-0.17506072446269627
1209	30	0.2834972052881426
1209	31	0.9447246030033156
1209	32	1.3525565156055777
1209	33	-0.8665643099134215
1209	34	-0.12903848649360583
1209	35	1.5149584086122587
1209	36	-0.4852487317464025
1209	37	1.3585850393210492
1209	38	0.8976012216627751
1209	39	0.7125400567193765
1209	40	-1.271566249327826
1209	41	0.4117543487536608
1209	42	0.41907570984281506
1209	43	0.9406272873100079
1209	44	-1.2063927597439204
1209	45	-0.6486895397075756
1209	46	1.4988206458359272
1209	47	0.9852439260328012
1209	48	1.755685247804422
1209	49	2.114286842436023
1209	50	1.1094112543802979
1209	51	0.6889862878349859
1214	1	-0.3403404550922957
1214	2	0.4837132637931987
1214	3	-0.5099858527883185
1214	4	1.4430638778790836
1214	5	-1.5208842152751743
1214	6	-0.8366021267228234
1214	7	-0.3280161667188821
1214	8	-1.5841432647831368
1214	9	-1.577127137017925
1214	10	-1.4310821477714057
1214	11	-0.8328803854115918
1214	12	-1.7859260841229272
1214	13	0.40796558881650213
1214	14	0.6837024570286943
1214	15	0.8241975263030383
1214	16	-0.059053920151043826
1214	17	1.6246211349333919
1214	18	1.0591891579084418
1214	19	0.9395679039581424
1214	20	-0.45318573922905614
1214	21	0.5937345868678108
1214	22	-0.8218128468914614
1214	23	0.41236264843740855
1214	24	-0.11438537691559247
1214	25	0.8966130601827658
1214	26	0.9339522500337907
1214	27	0.2492302699799609
1214	28	-0.5144195736028863
1214	29	0.8963678469658731
1214	30	-0.9545980328070911
1214	31	0.19075634903505545
1214	32	0.02715969020874809
1214	33	0.3953404519913448
1214	34	0.1884218309667105
1214	35	1.1657520594059108
1214	36	-1.1042963507940229
1214	37	0.8982675790035941
1214	38	0.17537899944055368
1214	39	-0.12079327661395922
1214	40	1.172878195116617
1214	41	0.11016704716636383
1214	42	-0.4460036552365523
1214	43	-2.54349969681698
1214	44	-1.595281648632806
1214	45	-0.267737158755196
1214	46	-0.12022697321169318
1214	47	-0.34015289936402837
1214	48	-1.204632212513033
1214	49	-1.687300459151274
1214	50	-2.533445888476848
1214	51	-2.509426410577717
1215	1	-0.06909862502693898
1215	2	1.1111642441853604
1215	3	0.8200795066887991
1215	4	-0.7530145534934647
1215	5	0.10329879126076946
1215	6	0.6535939517085438
1215	7	1.3974740293595502
1215	8	1.1282750358704519
1215	9	0.5601277649428553
1215	10	0.5362381136665064
1215	11	1.2390150394250128
1215	12	1.6748582296025631
1215	13	1.4798610136531067
1215	14	1.513767816505812
1215	15	1.4320406635579346
1215	16	0.7219918314829457
1215	17	-0.10086906114504046
1215	18	0.2225878507188952
1215	19	-0.28592229212028997
1215	20	0.3605397509670212
1215	21	-0.2755464588838237
1215	22	0.5278603557229218
1215	23	-0.46672231888285154
1215	24	1.313719198247803
1215	25	-0.609922887529649
1215	26	1.192122184674318
1215	27	1.1544590281499012
1215	28	0.9953843479657465
1215	29	0.4551913763776412
1215	30	1.6532451044478051
1215	31	1.7430439307344017
1215	32	1.3441531542610363
1215	33	-1.0556399401655199
1215	34	1.5054152950189987
1215	35	2.2376474842425154
1215	36	0.17021345312754477
1215	37	2.345979997304248
1215	38	-0.8671046606901669
1215	39	-0.31687170798650754
1215	40	0.4637278683192321
1215	41	1.3487291386696327
1215	42	1.2468068022797851
1215	43	0.9238205646209323
1215	44	0.20537194613843468
1215	45	-0.3167567665983313
1215	46	-1.0221877575254155
1215	47	-0.08198296472350108
1215	48	0.12543314696408459
1215	49	0.25714398529316895
1215	50	0.8194952879937389
1215	51	1.163776203801369
1212	1	-1.480863330909294
1212	2	-0.3006004616969946
1212	3	0.46713833021821216
1212	4	-1.7530145534934647
1212	5	0.8091811442019434
1212	6	0.5359468928850148
1212	7	-0.4848789118169208
1212	8	-2.930548493541309
1212	9	0.9130689414134423
1212	10	0.006826348960622397
1212	11	-1.0551026076338132
1212	12	-2.560435888044495
1212	13	-1.6966095745821903
1212	14	-2.42740865408242
1212	15	0.0790994870873476
1212	16	-2.9250669920464674
1212	17	-2.4538102376156274
1212	18	-0.07152979633992373
1212	19	-0.22709876270852192
1212	20	-1.6982837784447469
1212	21	0.37151236464558934
1212	22	-0.6486102325123753
1212	23	-2.1726046718240326
1212	24	-2.980398448811016
1212	25	-1.904040534588475
1212	26	-4.04317193297274
1212	27	-3.198482148320693
1212	28	-1.0046156520342535
1212	29	-2.42716156479883
1212	30	-1.8761666602580718
1212	31	-2.0804854810303013
1212	32	-1.7734939045624927
1212	33	-1.4085811166361069
1212	34	-2.9651729402751172
1212	35	-1.0564701628163107
1212	36	-2.300374782166571
1212	37	-1.8893141203428172
1212	38	-2.867104660690167
1212	39	-2.4345187668100365
1212	40	-2.124507425798413
1212	41	-1.0042120378009614
1212	42	-0.6355461388966859
1212	43	-2.1938264942025967
1212	44	-2.7358045244498044
1212	45	-1.2579332371865632
1212	46	-0.6104230516430604
1212	47	-1.611394729429385
1212	48	-0.16868450009473435
1212	49	-0.331091308824476
1212	50	-0.47462235906508
1212	51	-0.7774002667868629
1213	1	0.8342627195108747
1213	2	-0.8258105457306115
1213	3	-0.2639541067565787
1213	4	0.49068292549813464
1213	5	-1.203423897814858
1213	6	0.9729216828009868
1213	7	1.6243647856620669
1213	8	1.304745624105749
1213	9	-0.41046047035126065
1213	10	0.7673305506412973
1213	11	-0.5551026076338132
1213	12	-0.1192594174562629
1213	13	0.4714576523085654
1213	14	-0.4988372255109894
1213	15	-1.6519929498874433
1213	16	0.8774540163568929
1213	17	-0.5420455317332724
1213	18	0.35283995155923265
1213	19	0.5586155230057628
1213	20	0.28490949886617756
1213	21	1.657226650359874
1213	22	1.5194569943783804
1213	23	1.4996642357389973
1213	24	0.39355113102091366
1213	25	-0.13513297156326587
1213	26	1.8307776468591896
1213	27	0.36034138109107516
1213	28	-0.6810862402695506
1213	29	-0.032203581605557474
1213	30	-1.3593599375689962
1213	31	0.15901031728902382
1213	32	-0.29030062725156824
1213	33	2.204864261515155
1213	34	2.0852472277921095
1213	35	1.37210126575512
1213	36	-0.12810587460354128
1213	37	-0.5699863892503743
1213	38	-1.0309702069086555
1213	39	-1.6446028004234776
1213	40	-1.2001376778992565
1213	41	-0.3025313655320474
1213	42	1.1333614241285233
1213	43	-2.702229855547138
1213	44	-1.5635356168867744
1213	45	-0.005832396850429689
1213	46	-1.6440364970212116
1213	47	-0.4433275025386294
1213	48	-3.744314752195578
1213	49	-1.885713157563977
1213	50	-3.176303031333994
1213	51	-0.6681565693078753
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	1.0
22	4.0
23	12.0
24	18.0
25	36.0
26	78.0
27	92.0
28	100.0
29	160.0
30	212.0
31	238.0
32	275.0
33	325.0
34	411.0
35	548.0
36	680.0
37	973.0
38	1657.0
39	4413.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.30506155950752	10.30877467265976	12.057846394371701	60.32831737346102
2	7.289427398866523	12.184873949579831	67.96951338675005	12.556185264803595
3	9.360953683799101	15.995700605823727	62.429157709595465	12.214188000781707
4	6.8497166308383814	56.165722102794604	23.470783662302132	13.513777604064881
5	7.338284150869651	24.008207934336525	62.38030095759234	6.273206957201485
6	13.552863005667385	65.54621848739495	11.872190736759821	9.028727770177838
7	11.70607777994919	21.457885479773307	13.328121946453	53.507914793824504
8	10.54328708227477	65.69278874340434	10.660543287082275	13.103380887238616
9	11.2859097127223	15.18467852257182	12.673441469611099	60.855970295094785
10	8.452218096540943	61.38362321672856	18.868477623607582	11.295681063122924
11	14.207543482509283	14.627711549736174	59.42935313660348	11.735391831151066
12	10.56282978307602	14.58862614813367	20.53937854211452	54.309165526675784
13	12.25327340238421	12.78092632401798	59.75180769982411	15.213992573773696
14	9.312096931795974	14.080515927301155	16.640609732265	59.966777408637874
15	12.69298417041235	17.69591557553254	12.478014461598592	57.13308579245652
16	54.905217901113936	18.15516904436193	14.012116474496775	12.92749658002736
17	14.373656439319916	61.666992378346684	14.227086183310533	9.732264999022865
18	55.970295094782095	16.855579441078756	15.468047684189957	11.70607777994919
19	12.077389095172952	16.318155169044363	13.83623216728552	57.76822356849717
20	10.895055696697284	14.256400234512409	14.373656439319916	60.474887629470395
21	9.360953683799101	17.73500097713504	14.422513191323041	58.48153214774282
22	11.403165917529803	60.27946062145788	13.445378151260504	14.871995309751807
23	11.930818839163573	17.275747508305646	15.360562829783076	55.4328708227477
24	54.96384600351769	18.037912839554426	14.490912644127418	12.50732851280047
25	14.354113738518665	60.30877467265976	14.76451045534493	10.572601133476645
26	54.86613249951143	21.25268712136017	14.266171584913035	9.61500879421536
27	11.334766464725424	61.09048270470979	14.754739104944303	12.82001172562048
28	9.644322845417237	18.194254445964432	14.178229431307408	57.98319327731093
29	10.171975767051006	18.311510650771936	13.87531756888802	57.64119601328903
30	8.735587258159077	63.24017979284737	14.705882352941178	13.318350596052376
31	12.76138362321673	60.474887629470395	15.047879616963064	11.715849130349815
32	56.18526480359586	18.0281414891538	14.451827242524917	11.334766464725424
33	55.677154582763336	15.028336916161814	15.89798710181747	13.396521399257377
34	15.311706077779949	13.718975962478014	15.311706077779949	55.65761188196209
35	53.888997459448895	17.324604260308774	15.311706077779949	13.474692202462379
36	11.422708618331054	61.11979675591167	16.230213015438732	11.227281610318546
37	9.908149306234122	60.025405511041626	18.331053351573185	11.735391831151066
38	9.21438342778972	16.62106703146375	59.75180769982411	14.412741840922417
39	9.585694743013484	13.474692202462379	20.803205002931406	56.13640805159273
40	9.800664451827243	12.448700410396716	61.2859097127223	16.464725425053743
41	12.429157709595467	11.813562634356067	18.0281414891538	57.729138166894664
42	53.273402384209504	12.556185264803595	20.685948798123903	13.484463552863005
43	10.807113543091655	15.43873363298808	60.52374438147352	13.230408442446745
44	9.136212624584719	58.23724838772718	21.008403361344538	11.61813562634356
45	10.572601133476645	16.09341410982998	61.09048270470979	12.243502051983585
46	53.234316982607	14.0609732264999	17.88157123314442	14.82313855774868
47	10.992769200703536	13.376978698456126	15.937072503419971	59.69317959742037
48	10.338088723861638	13.797146765683015	15.868673050615595	59.996091459839754
49	52.384209497752586	13.005667383232364	18.389681453976937	16.220441665038106
50	9.233926128590971	13.533320304866134	61.35430916552668	15.87844440101622
51	7.054914989251515	13.103380887238616	20.480750439710768	59.3609536837991
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	1.0
4	1.0
5	0.5
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.5
14	3.0
15	2.5
16	2.0
17	4.0
18	6.0
19	4.0
20	2.0
21	1.0
22	0.0
23	1.5
24	3.0
25	4.5
26	8.0
27	10.0
28	17.0
29	24.0
30	66.5
31	109.0
32	191.5
33	274.0
34	410.0
35	546.0
36	674.5
37	803.0
38	843.0
39	883.0
40	774.0
41	665.0
42	555.0
43	445.0
44	360.5
45	276.0
46	240.0
47	204.0
48	229.5
49	255.0
50	241.0
51	227.0
52	474.5
53	722.0
54	2662.5
55	4603.0
56	2344.5
57	86.0
58	53.0
59	20.0
60	21.0
61	22.0
62	17.5
63	13.0
64	9.0
65	5.0
66	5.5
67	6.0
68	5.0
69	4.0
70	3.0
71	2.0
72	2.0
73	2.0
74	2.0
75	1.5
76	1.0
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	10234.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.25131913230408
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.60962072155411	36.59370725034199
2	9.875115633672525	8.344733242134064
3	2.0351526364477337	2.5796365057650967
4	0.6938020351526364	1.172562048075044
5	0.09250693802035154	0.19542700801250734
6	0.13876040703052728	0.35176861442251317
7	0.11563367252543941	0.3419972640218878
8	0.06938020351526364	0.2345124096150088
9	0.023126734505087884	0.08794215360562829
>10	0.2543940795559667	1.7099863201094392
>50	0.023126734505087884	0.7133085792456518
>100	0.04625346901017577	4.729333593902677
>500	0.0	0.0
>1k	0.023126734505087884	42.945085010748485
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC	4395	42.945085010748485	No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT	260	2.540551104162595	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT	224	2.188782489740082	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG	73	0.7133085792456518	No Hit
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACC	40	0.3908540160250147	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	24	0.2345124096150088	No Hit
ATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCTTC	15	0.1465702560093805	RNA PCR Primer, Index 46 (95% over 22bp)
CGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC	14	0.13679890560875513	No Hit
GTGTATAAGAGACAGCATCTCTTATACACATCTCCGAGCCCACGAGACCAA	14	0.13679890560875513	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC	13	0.12702755520812978	No Hit
GAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCAA	12	0.1172562048075044	No Hit
GGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCA	12	0.1172562048075044	No Hit
GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGA	11	0.10748485440687902	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.009771350400625366
8	0.0	0.0	0.009771350400625366
9	0.0	0.0	0.039085401602501464
10	0.0	0.0	0.039085401602501464
11	0.0	0.0	0.039085401602501464
12	0.0	0.0	0.039085401602501464
13	0.0	0.0	0.039085401602501464
14	0.0	0.0	0.039085401602501464
15	0.0	0.0	0.21496970881375807
16	0.0	0.0	0.27359781121751026
17	0.0	0.0	0.3224545632206371
18	0.0	0.0	0.6058237248387727
19	0.0	0.0	0.6644518272425249
20	0.0	0.0	0.7426226304475279
21	0.0	0.0	0.7817080320500294
22	0.0	0.0	0.8110220832519054
23	0.0	0.0	0.8207934336525308
24	0.0	0.0	0.9087355872581591
25	0.0	0.0	0.9478209888606606
26	0.0	0.0	1.0064490912644128
27	0.0	0.0	1.0259917920656634
28	0.0	0.0	1.0846198944694156
29	0.0	0.0	1.1921047488762948
30	0.0	0.0	1.3093609536837991
31	0.0	0.0	1.7099863201094392
32	0.0	0.0	2.286495993746336
33	0.0	0.0	3.087746726597616
34	0.0	0.0	4.113738518663279
35	0.0	0.0	4.836818448309557
36	0.0	0.0	5.618526480359586
37	0.0	0.0	6.693375024428376
38	0.0	0.0	7.787766269298417
39	0.0	0.0	8.852843462966582
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	20	6.709253E-4	45.0	45
TGCCGTC	415	0.0	42.831326	45
ATGCCGT	445	0.0	39.94382	44
ATCTCGT	460	0.0	39.619568	37
GCGAATC	460	0.0	39.619568	33
TCTCGTA	460	0.0	39.619568	38
CGAATCT	460	0.0	39.619568	34
CTCGTAT	460	0.0	39.619568	39
GGCGAAT	455	0.0	39.56044	32
AGGCGAA	455	0.0	39.56044	31
GTATGCC	460	0.0	39.130436	42
AATCTCG	460	0.0	39.130436	36
TATGCCG	460	0.0	39.130436	43
CGTATGC	460	0.0	39.130436	41
GAATCTC	460	0.0	39.130436	35
AAGGCGA	460	0.0	39.130436	30
TCGTATG	460	0.0	39.130436	40
CAAGGCG	460	0.0	39.130436	29
CCAAGGC	460	0.0	39.130436	28
AGACCAA	470	0.0	38.776596	25
>>END_MODULE