FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_62.34100000006bf3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_62.34100000006bf3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10234
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC439542.945085010748485No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT2602.540551104162595No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT2242.188782489740082No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG730.7133085792456518No Hit
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACC400.3908540160250147No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC240.2345124096150088No Hit
ATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCTTC150.1465702560093805RNA PCR Primer, Index 46 (95% over 22bp)
CGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC140.13679890560875513No Hit
GTGTATAAGAGACAGCATCTCTTATACACATCTCCGAGCCCACGAGACCAA140.13679890560875513No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC130.12702755520812978No Hit
GAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCAA120.1172562048075044No Hit
GGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCA120.1172562048075044No Hit
GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGA110.10748485440687902No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT206.709253E-445.045
TGCCGTC4150.042.83132645
ATGCCGT4450.039.9438244
ATCTCGT4600.039.61956837
GCGAATC4600.039.61956833
TCTCGTA4600.039.61956838
CGAATCT4600.039.61956834
CTCGTAT4600.039.61956839
GGCGAAT4550.039.5604432
AGGCGAA4550.039.5604431
GTATGCC4600.039.13043642
AATCTCG4600.039.13043636
TATGCCG4600.039.13043643
CGTATGC4600.039.13043641
GAATCTC4600.039.13043635
AAGGCGA4600.039.13043630
TCGTATG4600.039.13043640
CAAGGCG4600.039.13043629
CCAAGGC4600.039.13043628
AGACCAA4700.038.77659625
Produced by FastQC (version 0.11.2)