FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_61.34100000006be6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_61.34100000006be6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99279
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC19041.9178275365384423No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA2710.27296810000100724No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT2280.22965581845103195No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT1720.1732491261999013No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC1490.15008209188247262No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2250.037.99702545
GCGAATC2200.037.83794433
CGAGACC2200.037.83794423
CAAGGCG2150.037.67146329
AATCTCG2300.037.17136
AGGCGAA2250.036.997131
CGAATCT2250.036.997134
GGCGAAT2250.036.997132
GCCCACG2400.036.5780618
CTCGTAT2400.036.55963539
CCCACGA2400.036.55963519
TCTCGTA2400.036.55963538
ACGAGAC2400.036.55963522
CCACGAG2400.036.55963520
GACCAAG2250.035.9971826
GAGACCA2250.035.9971824
CCAAGGC2250.035.9971828
ACCAAGG2250.035.9971827
AAGGCGA2250.035.9971830
AGCCCAC2450.035.8315717
Produced by FastQC (version 0.11.2)