FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_60.34100000006bd9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_60.34100000006bd9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88771
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC15221.7145238873055388No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA3640.41004382061709344No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT2220.2500816708159196No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT1430.16108864381385812No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA1380.15545617375043652No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC1310.14757071566164626No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCAT1020.1149023892938009No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA940.10589043719232633No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTGT930.104763943179642No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAG910.10251095515427336No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT301.1314559E-437.49999645
ATGCCGT1900.036.71052644
CGAGACC1800.036.2523
CAAGGCG1750.036.00000429
CGAGCCC2000.036.00000415
GGCGAAT1750.036.00000432
CCACGAG2000.036.00000420
ACGAGAC1950.035.7692322
TGCCGTC1950.035.7692345
GCGAATC1700.035.73529433
CTCCGAG2100.035.35714312
TCCGAGC2100.035.35714313
TCTCCGA2100.035.35714311
GCCCACG2050.035.12195218
CCGAGCC2050.035.12195214
CGAATCT1800.035.034
CACGAGA2000.034.87500421
TATGCCG2000.034.87500443
CCCACGA2000.034.87500419
CTCGTAT1950.034.61538339