FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_6.3410000000687f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_6.3410000000687f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192041
Sequences flagged as poor quality0
Sequence length51
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAA35431.8449185330215945No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAA27621.4382345436651547No Hit
ACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAA19881.035195609270937No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19841.0331127207210962No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACC14790.77014804130368No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11480.5977890138043438No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATAC10030.5222843038726105No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA9420.49052025348753653No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCA8780.4571940366900818No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCA8330.43376154050437143No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA6770.3525288870605756No Hit
GTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAA6470.3369072229367687No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC6170.3212855588129618No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAG6080.31659905957581974No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC6070.3160783374383595No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATA5870.3056638946891549No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5630.2931665633901094No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAA5550.2890007862904276No Hit
GCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT5510.2869178977405866No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5260.27389984430408093No Hit
CGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5120.2666097343796377No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4940.25723673590535356No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4610.24005290536916593No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA4460.23224207330726251No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA4390.2285970183450409No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATC4240.22078618628313748No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCA4210.21922401987075676No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4180.21766185345837608No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCAACG4170.21714113132091586No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAGTACTCTG4160.21662040918345563No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAGTACTCT4160.21662040918345563No Hit
TACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTT4110.21401679849615446No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAGTACTCTGC3780.19683296795996685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAG3720.1937086351352055No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAGTACTCTGCG3620.1885014137606032No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG3510.18277347024854068No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAGTACTC3510.18277347024854068No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC3500.18225274811108044No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGAT3480.18121130383616No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.18121130383616No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTG3470.18069058169869975No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCA3310.1723590274993361No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG3300.17183830536187586No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTAT3270.17027613894949517No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAGTACTCTGCGT3250.16923469467457472No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT3240.16871397253711448No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGA3080.1603824183377508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC3070.15986169620029056No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCAA3040.15829952978790987No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAA2920.15205086413838712No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTT2910.15153014200092688No Hit
GATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT2900.15100941986346664No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTA2870.14944725345108595No Hit
GCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT2870.14944725345108595No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTC2840.14788508703870526No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATC2820.1468436427637848No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC2820.1468436427637848RNA PCR Primer, Index 27 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2790.14528147635140412No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAGTACT2780.14476075421394388No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCAC2770.14424003207648367No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG2690.14007425497680184No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTT2690.14007425497680184No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTG2680.1395535328393416No Hit
GTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2660.13851208856442115No Hit
CCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCG2660.13851208856442115No Hit
TACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTC2650.13799136642696092No Hit
CTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAA2640.13747064428950068No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTG2600.13538775573965978No Hit
ATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2580.1343463114647393No Hit
ACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGTAC2510.1307012565025177No Hit
AAAAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAA2460.12809764581521654No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTGCGCAAGC2460.12809764581521654No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA2450.12757692367775633No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAGTAC2450.12757692367775633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG2440.1270562015402961No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGA2420.12601475726537564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCA2380.1239318687155347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCA2370.12341114657807446No Hit
TATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGA2320.12080753589077332No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTT2300.11976609161585287No Hit
GATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2300.11976609161585287No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTGCGCAAG2300.11976609161585287No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC2260.11768320306601195No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC2230.11612103665363126No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTGCGCAA2170.11299670382886988No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.11091381527902895No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT2120.11039309314156874No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTG2120.11039309314156874No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAGTACTCTGCGT2100.10935164886664826No Hit
ACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGTACT2060.10726876031680735No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAGTACTCT2040.10622731604188689No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCT2010.1046651496295062No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTAT2000.10414442749204597No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGT2000.10414442749204597No Hit
ACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGTA2000.10414442749204597No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTGCGCAAGCAG1970.10258226107966528No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCACT1930.10049937252982435No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1930.10049937252982435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC551.8189894E-1244.9997442
ATCTCGT551.8189894E-1244.9997437
TGCCGTC551.8189894E-1244.9997445
TATGCCG551.8189894E-1244.9997443
GCTATCT351.2049168E-744.9997434
TCTCGTA551.8189894E-1244.9997438
ATTGCTA351.2049168E-744.9997431
ATGCCGT551.8189894E-1244.9997444
CGTATGC551.8189894E-1244.9997441
TGCTATC351.2049168E-744.9997433
TCGTATG551.8189894E-1244.9997440
AATTGCT351.2049168E-744.9997430
CTATCTC351.2049168E-744.9997435
TTGCTAT351.2049168E-744.9997432
TATCTCG453.8198777E-1044.99973736
CGAATTG406.7648216E-944.99973728
AGACGAA406.7648216E-944.99973725
GAATTGC406.7648216E-944.99973729
GACGAAT406.7648216E-944.99973726
ACGAATT406.7648216E-944.99973727
Produced by FastQC (version 0.11.2)