##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_6.3410000000687f.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 192041 Sequences flagged as poor quality 0 Sequence length 51 %GC 32 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.56377023656407 38.0 38.0 38.0 32.0 38.0 2 36.41399492816638 38.0 38.0 38.0 32.0 38.0 3 36.41363562989153 38.0 38.0 38.0 32.0 38.0 4 36.43702646830625 38.0 38.0 38.0 32.0 38.0 5 36.40874084179941 38.0 38.0 38.0 32.0 38.0 6 38.40704849485266 40.0 38.0 40.0 38.0 40.0 7 38.37122801901677 40.0 38.0 40.0 38.0 40.0 8 38.35685608802287 40.0 38.0 40.0 38.0 40.0 9 38.388713868392685 40.0 38.0 40.0 38.0 40.0 10 38.35827765945814 40.0 38.0 40.0 38.0 40.0 11 38.24027681588827 40.0 38.0 40.0 38.0 40.0 12 38.30676782562057 40.0 38.0 40.0 38.0 40.0 13 38.188595143745346 40.0 38.0 40.0 38.0 40.0 14 38.168510890903505 40.0 38.0 40.0 38.0 40.0 15 38.060565191808 40.0 38.0 40.0 32.0 40.0 16 38.01346587447472 40.0 38.0 40.0 32.0 40.0 17 38.14116777146547 40.0 38.0 40.0 38.0 40.0 18 38.051181778890964 40.0 38.0 40.0 32.0 40.0 19 38.18389823006545 40.0 38.0 40.0 38.0 40.0 20 38.156565525070164 40.0 38.0 40.0 38.0 40.0 21 38.20235262261704 40.0 38.0 40.0 38.0 40.0 22 38.23192964002479 40.0 38.0 40.0 38.0 40.0 23 38.1940366900818 40.0 38.0 40.0 38.0 40.0 24 38.14756223931348 40.0 38.0 40.0 32.0 40.0 25 38.086762722543625 40.0 38.0 40.0 32.0 40.0 26 37.93367041413032 40.0 38.0 40.0 32.0 40.0 27 37.737649772704785 40.0 38.0 40.0 32.0 40.0 28 37.530282595904 40.0 38.0 40.0 32.0 40.0 29 37.24192750506402 40.0 38.0 40.0 32.0 40.0 30 36.901833462645996 40.0 38.0 40.0 32.0 40.0 31 36.56352549715946 40.0 38.0 40.0 27.0 40.0 32 36.14834852974104 40.0 38.0 40.0 27.0 40.0 33 35.6929718133107 40.0 38.0 40.0 27.0 40.0 34 35.14406298654975 38.0 38.0 40.0 13.0 40.0 35 34.52518472617827 38.0 32.0 40.0 13.0 40.0 36 33.86005592555756 38.0 32.0 40.0 13.0 40.0 37 33.173140110705525 38.0 27.0 40.0 13.0 40.0 38 32.431741138611024 38.0 27.0 40.0 13.0 40.0 39 31.585151087528185 38.0 27.0 40.0 13.0 40.0 40 30.76590415588338 38.0 27.0 40.0 13.0 40.0 41 29.976312349966935 38.0 13.0 40.0 13.0 40.0 42 29.158315151452033 32.0 13.0 40.0 13.0 40.0 43 28.334511901104452 32.0 13.0 38.0 13.0 40.0 44 27.605297827026522 32.0 13.0 38.0 13.0 40.0 45 26.927150972969315 27.0 13.0 38.0 13.0 40.0 46 26.231070448497977 27.0 13.0 38.0 13.0 40.0 47 25.68398935643951 27.0 13.0 38.0 13.0 40.0 48 25.14188116079379 27.0 13.0 38.0 13.0 40.0 49 24.76338386073807 27.0 13.0 38.0 13.0 40.0 50 24.401669435172696 27.0 13.0 38.0 13.0 40.0 51 23.241880640071653 13.0 13.0 38.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.34636407203987574 2201 2 0.045088194776127466 2201 3 0.19193684751623152 2201 4 -0.17217908370957247 2201 5 -0.04678994843611406 2201 6 0.3068822909884119 2201 7 0.028722075958327764 2201 8 0.2332309359418545 2201 9 0.5739439299550284 2201 10 0.1339822359440248 2201 11 0.4347348421945014 2201 12 0.28920343036417506 2201 13 0.44791686101869743 2201 14 0.15200381167575472 2201 15 0.22576952880952206 2201 16 0.29027474075823534 2201 17 0.5614299525436195 2201 18 0.4117502188947242 2201 19 -0.14639843908891947 2201 20 0.26136924135546025 2201 21 0.24064767192733427 2201 22 0.2330939811236874 2201 23 0.11944857708395773 2201 24 -0.14241836576096034 2201 25 -0.19231287094221017 2201 26 -0.3120531232181065 2201 27 -0.40745298333563085 2201 28 -0.6425490322351664 2201 29 -0.20111238196733439 2201 30 -0.40255846858675426 2201 31 -0.33570319798491255 2201 32 -0.9366474751384288 2201 33 -0.6264723041983444 2201 34 -0.4360406343596779 2201 35 -1.4104875508264456 2201 36 -1.3583452178460078 2201 37 -1.2966649543717743 2201 38 -0.8430049239187625 2201 39 -0.4825358004564393 2201 40 -1.1041254606644344 2201 41 -1.2769101592045757 2201 42 -0.5362168925822033 2201 43 -0.39945737892540834 2201 44 -0.9692846171528515 2201 45 -0.6070836728966462 2201 46 0.1460719848555705 2201 47 -0.8586482663394825 2201 48 -1.0471650892755981 2201 49 -1.6349631219429348 2201 50 -1.181224558068525 2201 51 -1.3456648361381376 1101 1 0.7888871286933679 1101 2 0.6468668509026116 1101 3 0.9113044364490435 1101 4 0.6937629452759353 1101 5 0.40792019649141764 1101 6 0.6359336743876227 1101 7 0.5942688480268359 1101 8 0.7707803430564795 1101 9 0.7369874082158958 1101 10 0.5903392846923836 1101 11 0.7468231162392911 1101 12 0.7287620601401912 1101 13 0.8118826054192212 1101 14 0.5887627049563875 1101 15 0.5554796737370538 1101 16 0.40736960241832065 1101 17 0.6055669749415102 1101 18 0.47153282759037296 1101 19 0.8671061722417832 1101 20 0.5703283981407026 1101 21 0.7101799512422247 1101 22 0.5071387769076168 1101 23 0.9601929776109728 1101 24 0.9685829517620945 1101 25 1.0968834399932348 1101 26 1.1327755987845265 1101 27 1.2845760021716188 1101 28 0.9743811390428334 1101 29 1.104552967176275 1101 30 1.288647065010089 1101 31 0.9055682117647592 1101 32 0.6353222218312666 1101 33 -0.3005500380586881 1101 34 -0.26031599667852134 1101 35 0.015401777236796477 1101 36 0.8573991826810001 1101 37 0.4790267452329715 1101 38 0.11598717094290123 1101 39 -0.30055292825617386 1101 40 -0.7566287544720787 1101 41 -0.2843212263982515 1101 42 -1.418792650157961 1101 43 -0.6675733209543928 1101 44 -0.9137839583913241 1101 45 -0.10823650557122022 1101 46 -0.01104263965035912 1101 47 0.3315691249648651 1101 48 -0.7210781327538598 1101 49 0.1076258108633894 1101 50 -0.8928516990434936 1101 51 -1.135355218219825 2202 1 0.2528805107901988 2202 2 0.15722909402347085 2202 3 0.09556079311919063 2202 4 0.2781412106886876 2202 5 0.4632580578637615 2202 6 0.13097024701040283 2202 7 -0.21041593079587528 2202 8 0.2211460692842735 2202 9 0.014678108320389072 2202 10 0.47108641112916416 2202 11 -0.22248200392789386 2202 12 0.22001075397509595 2202 13 -0.2303500661237976 2202 14 0.14553734793792472 2202 15 -0.04196024266838094 2202 16 0.2558979897398075 2202 17 0.19717268723687909 2202 18 0.443189044936247 2202 19 0.3466429190199065 2202 20 0.3792799829543583 2202 21 0.1537971577904642 2202 22 0.1253902572280623 2202 23 0.2614381883389427 2202 24 -0.14818820811251499 2202 25 -0.09555821796566732 2202 26 -0.038114335036439684 2202 27 0.01350320523675208 2202 28 0.41351384541691516 2202 29 0.2271583311609504 2202 30 0.2717104575562388 2202 31 0.4394849655090951 2202 32 -0.007354545845494442 2202 33 -0.0899526852199557 2202 34 -0.08663957731244665 2202 35 -0.07872918758382497 2202 36 0.3938847277872384 2202 37 0.4056202666540756 2202 38 0.22888337435634298 2202 39 -0.39429204354701497 2202 40 -0.49426372486566805 2202 41 -0.8480185444059138 2202 42 -0.6180699054696994 2202 43 -0.1355287371856697 2202 44 -0.32309256166544387 2202 45 -0.27633995384708143 2202 46 -0.7371833571703874 2202 47 -0.38608151940991675 2202 48 0.07672750987936894 2202 49 0.11611588400863582 2202 50 -0.37800495574847304 2202 51 -1.563646754269982 1102 1 -0.21557415076791386 1102 2 -0.006500152464390396 1102 3 -0.126406001261401 1102 4 0.0417427432557389 1102 5 -0.28795522438399956 1102 6 -0.23224814379419456 1102 7 0.3018446056025965 1102 8 0.292665864942002 1102 9 0.32974835097684263 1102 10 0.10978372913682222 1102 11 0.12451671893289529 1102 12 -0.07654097016283856 1102 13 0.026865770402388023 1102 14 -0.06914975295607206 1102 15 0.01793758619496799 1102 16 -0.3738593538106372 1102 17 -0.08635221697768003 1102 18 0.030034511092061678 1102 19 -0.06370190856630131 1102 20 0.1359849637972701 1102 21 -0.08081331475104037 1102 22 0.2711118408806783 1102 23 -0.08888109646310483 1102 24 -0.2834372502581033 1102 25 -0.1680997249899292 1102 26 -0.7331334921245656 1102 27 -0.2172758496802345 1102 28 -0.16349764883595697 1102 29 -0.46211369949039494 1102 30 -0.5119421605791388 1102 31 -0.6414856602891135 1102 32 -0.5184656569566073 1102 33 0.0922950797864317 1102 34 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19 0.09509776702169859 2203 20 0.15382762397569394 2203 21 0.2748503781389289 2203 22 0.13896801822590987 2203 23 -0.043760793678565335 2203 24 0.4111288486875537 2203 25 0.149526660519669 2203 26 -0.11658847995911259 2203 27 0.015111281976970758 2203 28 0.13723227817134642 2203 29 -0.23243000119355628 2203 30 -0.07971723492355665 2203 31 -0.3846054223869828 2203 32 -0.3812393795843434 2203 33 -0.947337253276558 2203 34 -1.03683114819367 2203 35 -0.9509220449264433 2203 36 -1.6891060152840467 2203 37 -0.5214709322720097 2203 38 -0.7412623313795983 2203 39 -0.3508460050091067 2203 40 0.08549688144917766 2203 41 -0.3582263358873021 2203 42 -1.58167143803675 2203 43 -1.1792963910716239 2203 44 -0.8343490988469746 2203 45 -1.3275187440140428 2203 46 -0.7285277093252098 2203 47 -1.2960851550616788 2203 48 -0.418490209727981 2203 49 -0.7842952243058221 2203 50 -1.3694332075593074 2203 51 -0.3788577619048503 1103 1 0.09284097814191483 1103 2 0.27833167583033 1103 3 0.3073853873003074 1103 4 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0.47161008791575654 2204 26 0.5019180542671862 2204 27 0.6617237684946389 2204 28 0.5773958219544255 2204 29 0.22826430738246017 2204 30 0.4391078585121164 2204 31 0.932473866247733 2204 32 0.9640085698475147 2204 33 0.7864149728753986 2204 34 0.41677709922900874 2204 35 0.8714623832974056 2204 36 0.6024007446928721 2204 37 0.4461463953423106 2204 38 0.3627459963099682 2204 39 0.9406607549678156 2204 40 1.1083353192548842 2204 41 1.1284794608869717 2204 42 0.5918680808635948 2204 43 0.226678475359261 2204 44 0.44864184604479007 2204 45 0.46178067655936417 2204 46 0.6374265887157762 2204 47 -0.318735467350038 2204 48 -0.19202471194786241 2204 49 -0.45575282081421165 2204 50 -1.1484358914833557 2204 51 -1.07330429663282 2205 1 0.48630930265137806 2205 2 0.05991370166277221 2205 3 -0.0905571831600227 2205 4 0.06900857564102125 2205 5 0.42970373150021146 2205 6 0.24351977429454053 2205 7 0.21959204587562908 2205 8 0.07701676874683017 2205 9 0.2639033250635592 2205 10 0.11664758958430355 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1.3306332828434648 1213 50 1.941965652554682 1213 51 1.7104833039885605 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 2.0 16 3.0 17 4.0 18 6.0 19 17.0 20 50.0 21 108.0 22 269.0 23 565.0 24 1074.0 25 1803.0 26 2913.0 27 4157.0 28 5354.0 29 6799.0 30 8962.0 31 11903.0 32 14851.0 33 17292.0 34 19210.0 35 19282.0 36 18295.0 37 17392.0 38 18686.0 39 23044.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.319025691246644 26.417394203321315 11.909028399974996 13.354551705457046 2 10.075662502538574 29.64949462863927 30.137942167394826 30.136900701427326 3 36.59166532146781 33.95681130591905 21.61413448169922 7.837388890913919 4 16.44857087809374 18.818898047812706 36.90722293676871 27.825308137324846 5 11.480881686723148 27.998187886961638 40.430428918824624 20.090501507490586 6 40.23151306231482 31.64115996063341 9.42090491093048 18.7064220661213 7 9.772913075853594 39.58477616758921 26.508401851687918 24.133908904869273 8 20.111330392988997 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10.634187491212813 36.0287646908733 37.08739279632995 16.249655021583933 22 4.6734811837055625 38.1132154071266 38.514692175108436 18.698611234059392 23 3.354492009518801 39.2509932774772 44.765440713181036 12.629073999822953 24 4.9244692539613935 44.45925609635443 45.26429252086794 5.351982128816243 25 8.414349019219854 39.03801792325597 42.78982092365693 9.757812133867247 26 5.795146844407415 38.26129972922308 47.89106436159133 8.052489064778172 27 6.858431272488687 41.02196926697945 47.378945121093935 4.740654339437932 28 4.338656849318635 41.68328638155394 46.680136012622306 7.297920756505122 29 6.209611489213241 41.541129238027295 46.88634197905655 5.362917293702908 30 6.505936263278484 41.07946261195584 46.284107477608835 6.130493647156842 31 9.569831442244105 40.9048067860509 42.40292437552397 7.122437396181024 32 7.885816049697721 41.837420134242166 41.25421134028671 9.022552475773402 33 9.953603657552293 42.948641175582296 39.7722361370749 7.325519029790513 34 9.681786701798053 42.9918611129915 38.57197161022906 8.754380574981385 35 10.432147301878244 44.18379408563796 37.18320566962263 8.200852942861161 36 10.926312610328003 44.19681213907447 35.92774459620602 8.94913065439151 37 12.147926744809702 43.77242359704438 34.609276144156716 9.4703735139892 38 13.21019990522857 42.26961950833415 34.26664097770789 10.253539608729385 39 14.823917809217823 41.046963929577544 33.18249748751569 10.946620773688952 40 16.383565923765882 40.08852322432826 32.222453655488444 11.305457196417414 41 17.14642782753593 39.54384503228494 30.590501978754425 12.719225161424703 42 17.860248592748423 38.74589280414078 29.845710030670535 13.54814857244026 43 18.410651892043887 37.61592576585208 29.40257549169188 14.57084685041215 44 18.380970730208652 36.669773642086845 29.46974864742425 15.479506980280252 45 19.41876995016689 35.851198441999365 28.346030274785072 16.384001333048673 46 20.31857780369817 35.71268635343494 26.89321551127103 17.075520331595857 47 20.537281101431464 34.64260236095417 26.444873750917775 18.375242786696592 48 20.86689821444379 33.66468618680386 26.284491332580025 19.18392426617233 49 21.24754609692722 32.44307205232216 26.227732619596857 20.081649231153765 50 21.286079535099276 31.532329033904215 25.97830671575341 21.203284715243097 51 22.65813358883965 31.46476980998453 24.51194301098226 21.365153590193557 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 15.0 1 18.0 2 21.0 3 52.0 4 83.0 5 96.0 6 109.0 7 82.5 8 56.0 9 65.5 10 75.0 11 185.0 12 295.0 13 422.5 14 550.0 15 784.5 16 1019.0 17 1360.0 18 1701.0 19 2326.5 20 2952.0 21 3836.5 22 4721.0 23 5865.0 24 7009.0 25 8855.5 26 12430.5 27 14159.0 28 18629.0 29 23099.0 30 26724.0 31 30349.0 32 25389.5 33 20430.0 34 19577.0 35 18724.0 36 18437.0 37 18150.0 38 18750.5 39 19351.0 40 16179.5 41 13008.0 42 7913.5 43 2819.0 44 1803.0 45 787.0 46 574.5 47 362.0 48 429.5 49 497.0 50 562.0 51 627.0 52 398.0 53 169.0 54 122.0 55 75.0 56 49.5 57 24.0 58 23.0 59 22.0 60 19.5 61 17.0 62 13.5 63 10.0 64 13.5 65 17.0 66 12.5 67 8.0 68 7.0 69 6.0 70 7.0 71 8.0 72 6.0 73 4.0 74 4.0 75 3.0 76 2.0 77 1.5 78 1.0 79 1.5 80 2.0 81 1.5 82 1.0 83 0.5 84 0.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03592982748475586 2 0.0020828885498409195 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0031243328247613786 12 0.0 13 0.0 14 0.0 15 0.0 16 5.207221374602299E-4 17 0.0010414442749204598 18 0.0026036106873011493 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 5.207221374602299E-4 27 0.0 28 0.0 29 0.0 30 5.207221374602299E-4 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 5.207221374602299E-4 41 5.207221374602299E-4 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0020828885498409195 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 192041.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.98241466446923 #Duplication Level Percentage of deduplicated Percentage of total 1 84.96779538674927 33.972176282787856 2 6.86230357566609 5.48742934231503 3 2.4640251403880176 2.9555302472001226 4 1.344748859844235 2.150652261354577 5 0.8800689926236689 1.7593641698210625 6 0.47832899226944386 1.1474848868972762 7 0.39853694168393683 1.115412848306159 8 0.3057114429296579 0.9778465343109444 9 0.2172281135219919 0.7816774060455007 >10 1.5883726677017016 12.88975241823311 >50 0.2526519561458313 7.095486797625989 >100 0.21339566242602603 17.912157213316018 >500 0.017888178700087035 4.650414357845781 >1k 0.008944089350043517 7.104615233940599 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAA 3543 1.8449185330215945 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAA 2762 1.4382345436651547 No Hit ACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAA 1988 1.035195609270937 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1984 1.0331127207210962 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACC 1479 0.77014804130368 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1148 0.5977890138043438 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATAC 1003 0.5222843038726105 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA 942 0.49052025348753653 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCA 878 0.4571940366900818 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCA 833 0.43376154050437143 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA 677 0.3525288870605756 No Hit GTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAA 647 0.3369072229367687 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC 617 0.3212855588129618 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAG 608 0.31659905957581974 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC 607 0.3160783374383595 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATA 587 0.3056638946891549 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 563 0.2931665633901094 No Hit AAAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAA 555 0.2890007862904276 No Hit GCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT 551 0.2869178977405866 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 526 0.27389984430408093 No Hit CGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 512 0.2666097343796377 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 494 0.25723673590535356 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 461 0.24005290536916593 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA 446 0.23224207330726251 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA 439 0.2285970183450409 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATC 424 0.22078618628313748 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCA 421 0.21922401987075676 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 418 0.21766185345837608 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCAACG 417 0.21714113132091586 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAGTACTCTG 416 0.21662040918345563 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAGTACTCT 416 0.21662040918345563 No Hit TACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTT 411 0.21401679849615446 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAGTACTCTGC 378 0.19683296795996685 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAG 372 0.1937086351352055 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAGTACTCTGCG 362 0.1885014137606032 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG 351 0.18277347024854068 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAGTACTC 351 0.18277347024854068 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC 350 0.18225274811108044 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGAT 348 0.18121130383616 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 348 0.18121130383616 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTG 347 0.18069058169869975 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCA 331 0.1723590274993361 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG 330 0.17183830536187586 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTAT 327 0.17027613894949517 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAGTACTCTGCGT 325 0.16923469467457472 No Hit TACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT 324 0.16871397253711448 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGA 308 0.1603824183377508 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC 307 0.15986169620029056 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCAA 304 0.15829952978790987 No Hit AAAAAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAA 292 0.15205086413838712 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTT 291 0.15153014200092688 No Hit GATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT 290 0.15100941986346664 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTA 287 0.14944725345108595 No Hit GCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT 287 0.14944725345108595 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTC 284 0.14788508703870526 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATC 282 0.1468436427637848 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 282 0.1468436427637848 RNA PCR Primer, Index 27 (95% over 21bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 279 0.14528147635140412 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAGTACT 278 0.14476075421394388 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCAC 277 0.14424003207648367 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG 269 0.14007425497680184 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTT 269 0.14007425497680184 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTG 268 0.1395535328393416 No Hit GTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 266 0.13851208856442115 No Hit CCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCG 266 0.13851208856442115 No Hit TACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTC 265 0.13799136642696092 No Hit CTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAA 264 0.13747064428950068 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTG 260 0.13538775573965978 No Hit ATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 258 0.1343463114647393 No Hit ACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGTAC 251 0.1307012565025177 No Hit AAAAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAA 246 0.12809764581521654 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTGCGCAAGC 246 0.12809764581521654 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA 245 0.12757692367775633 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAGTAC 245 0.12757692367775633 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG 244 0.1270562015402961 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGA 242 0.12601475726537564 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCA 238 0.1239318687155347 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCA 237 0.12341114657807446 No Hit TATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGA 232 0.12080753589077332 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTT 230 0.11976609161585287 No Hit GATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 230 0.11976609161585287 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTGCGCAAG 230 0.11976609161585287 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC 226 0.11768320306601195 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC 223 0.11612103665363126 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTGCGCAA 217 0.11299670382886988 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 213 0.11091381527902895 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT 212 0.11039309314156874 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTG 212 0.11039309314156874 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAGTACTCTGCGT 210 0.10935164886664826 No Hit ACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGTACT 206 0.10726876031680735 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAGTACTCT 204 0.10622731604188689 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCT 201 0.1046651496295062 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTAT 200 0.10414442749204597 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGT 200 0.10414442749204597 No Hit ACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGTA 200 0.10414442749204597 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTGCGCAAGCAG 197 0.10258226107966528 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCACT 193 0.10049937252982435 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 193 0.10049937252982435 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 5.207221374602299E-4 10 0.0 0.0 5.207221374602299E-4 11 0.0 0.0 0.0010414442749204598 12 0.0 0.0 0.0010414442749204598 13 0.0 0.0 0.0020828885498409195 14 0.0 0.0 0.0026036106873011493 15 0.0 0.0 0.003124332824761379 16 0.0 0.0 0.006248665649522758 17 0.0 0.0 0.010414442749204597 18 0.0 0.0 0.013018053436505746 19 0.0 0.0 0.016142386261267124 20 0.0 0.0 0.019266719086028505 21 0.0 0.0 0.024994662598091032 22 0.0 0.0 0.03280549465999448 23 0.0 0.0 0.03697127175967632 24 0.0 0.0 0.044782103821579766 25 0.0 0.0 0.04530282595904 26 0.0 0.0 0.048427158783801376 27 0.0 0.0 0.057800157258085516 28 0.0 0.0 0.06873532214475034 29 0.0 0.0 0.08227409771871631 30 0.0 0.0 0.09320926260538115 31 0.0 0.0 0.11872464734093241 32 0.0 0.0 0.1463229206263246 33 0.0 0.0 0.1692346946745747 34 0.0 0.0 0.19631224582250664 35 0.0 0.0 0.23224207330726251 36 0.0 0.0 0.2666097343796377 37 0.0 0.0 0.3087882275139163 38 0.0 0.0 0.3400315557615301 39 0.0 0.0 0.40251821225675766 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGCC 55 1.8189894E-12 44.99974 42 ATCTCGT 55 1.8189894E-12 44.99974 37 TGCCGTC 55 1.8189894E-12 44.99974 45 TATGCCG 55 1.8189894E-12 44.99974 43 GCTATCT 35 1.2049168E-7 44.99974 34 TCTCGTA 55 1.8189894E-12 44.99974 38 ATTGCTA 35 1.2049168E-7 44.99974 31 ATGCCGT 55 1.8189894E-12 44.99974 44 CGTATGC 55 1.8189894E-12 44.99974 41 TGCTATC 35 1.2049168E-7 44.99974 33 TCGTATG 55 1.8189894E-12 44.99974 40 AATTGCT 35 1.2049168E-7 44.99974 30 CTATCTC 35 1.2049168E-7 44.99974 35 TTGCTAT 35 1.2049168E-7 44.99974 32 TATCTCG 45 3.8198777E-10 44.999737 36 CGAATTG 40 6.7648216E-9 44.999737 28 AGACGAA 40 6.7648216E-9 44.999737 25 GAATTGC 40 6.7648216E-9 44.999737 29 GACGAAT 40 6.7648216E-9 44.999737 26 ACGAATT 40 6.7648216E-9 44.999737 27 >>END_MODULE