FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_59.34100000006bcc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_59.34100000006bcc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90470
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC10271.1351829335691388No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA1360.1503260749419697No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT1120.12379794406985742No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT1070.11827125013816735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1350.041.66666445
CGTATGC1400.040.1785741
CTCGTAT1400.040.1785739
CCCACGA1400.040.1785719
TCTCGTA1400.040.1785738
CCGAGCC1400.040.1785714
TATGCCG1450.038.79310243
GTATGCC1450.038.79310242
CCACGAG1450.038.79310220
AATCTCG1400.038.57142636
GCGAATC1350.038.33333233
ATCTCGT1500.037.50000437
CTCCGAG1500.037.50000412
CACGAGA1500.037.50000421
TCGTATG1500.037.50000440
CGAGCCC1500.037.50000415
ATGCCGT1500.037.50000444
ACGAGAC1500.037.50000422
CGAATCT1450.037.2413834
CGAGACC1450.037.2413823