FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_57.34100000006ba3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_57.34100000006ba3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100102
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC3790.37861381390981197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCAAT206.985585E-445.0219881
CTCTTAG300.005112861330.014661
ATACTGG300.00512537929.9996666
TAACCAG300.00512537929.9996665
CGAATCT300.00512537929.99966634
TGCCGTC300.00512537929.99966645
GCGAATC406.1269815E-428.12468733
ACAGCTC608.095052E-626.2497088
ATCAACG651.50590495E-524.2305012
TCAACGC651.50590495E-524.2305013
CAACGCA702.6715212E-522.4997484
AGAGTAC702.6715212E-522.49974810
GCACAGC702.6715212E-522.4997486
AACGCAG702.6715212E-522.4997485
ACGCAGA754.5493543E-520.9997676
CACAGCT754.5493543E-520.9997677
CGCAGAG754.5493543E-520.9997677
AGTGTGC654.2014188E-420.7690018
TGCACAG750.0011007117.99985
TATCAAC901.8374137E-417.508551
Produced by FastQC (version 0.11.2)