##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_56.34100000006b96.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 64749 Sequences flagged as poor quality 0 Sequence length 51 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.33595885650744 38.0 38.0 38.0 32.0 38.0 2 36.386894006085036 38.0 38.0 38.0 32.0 38.0 3 36.32625986501722 38.0 38.0 38.0 32.0 38.0 4 36.437134164234195 38.0 38.0 38.0 32.0 38.0 5 36.4295201470293 38.0 38.0 38.0 32.0 38.0 6 38.31344113422601 40.0 38.0 40.0 38.0 40.0 7 38.21978717818036 40.0 38.0 40.0 38.0 40.0 8 38.21890685570433 40.0 38.0 40.0 38.0 40.0 9 38.22621198782993 40.0 38.0 40.0 38.0 40.0 10 38.16945435450741 40.0 38.0 40.0 38.0 40.0 11 38.0893141206814 40.0 38.0 40.0 38.0 40.0 12 38.00858700520472 40.0 38.0 40.0 32.0 40.0 13 38.07473474493815 40.0 38.0 40.0 38.0 40.0 14 37.99034734127168 40.0 38.0 40.0 32.0 40.0 15 38.03728242907226 40.0 38.0 40.0 32.0 40.0 16 38.04449489567406 40.0 38.0 40.0 38.0 40.0 17 38.036340329580376 40.0 38.0 40.0 32.0 40.0 18 38.02318182520193 40.0 38.0 40.0 32.0 40.0 19 38.08329086163493 40.0 38.0 40.0 38.0 40.0 20 38.091445427728615 40.0 38.0 40.0 38.0 40.0 21 38.094179060680474 40.0 38.0 40.0 38.0 40.0 22 38.08818669014193 40.0 38.0 40.0 38.0 40.0 23 38.048016185578156 40.0 38.0 40.0 32.0 40.0 24 38.03912029529413 40.0 38.0 40.0 32.0 40.0 25 38.017652782282354 40.0 38.0 40.0 32.0 40.0 26 37.96103414724552 40.0 38.0 40.0 32.0 40.0 27 37.958470401087276 40.0 38.0 40.0 32.0 40.0 28 37.92370538541136 40.0 38.0 40.0 32.0 40.0 29 37.92412238026842 40.0 38.0 40.0 32.0 40.0 30 37.872322352468764 40.0 38.0 40.0 32.0 40.0 31 37.89080912446524 40.0 38.0 40.0 32.0 40.0 32 37.88121824275279 40.0 38.0 40.0 32.0 40.0 33 37.84387403666466 40.0 38.0 40.0 32.0 40.0 34 37.86844584472347 40.0 38.0 40.0 32.0 40.0 35 37.85100928199663 40.0 38.0 40.0 32.0 40.0 36 37.78756428670713 40.0 38.0 40.0 32.0 40.0 37 37.79565707578495 40.0 38.0 40.0 32.0 40.0 38 37.7992864754668 40.0 38.0 40.0 32.0 40.0 39 37.72957111306739 40.0 38.0 40.0 32.0 40.0 40 37.6739100217764 40.0 38.0 40.0 32.0 40.0 41 37.707593939674744 40.0 38.0 40.0 32.0 40.0 42 37.65332283124064 40.0 38.0 40.0 32.0 40.0 43 37.70530819008788 40.0 38.0 40.0 32.0 40.0 44 37.660334522540886 40.0 38.0 40.0 32.0 40.0 45 37.62465829588102 40.0 38.0 40.0 32.0 40.0 46 37.53877279957992 40.0 38.0 40.0 32.0 40.0 47 37.54053344453196 40.0 38.0 40.0 32.0 40.0 48 37.45738158118272 40.0 38.0 40.0 32.0 40.0 49 37.40525722404979 40.0 38.0 40.0 32.0 40.0 50 37.41531143338121 40.0 38.0 40.0 32.0 40.0 51 36.96966748521213 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.7276053254404289 2201 2 -0.11499648714372768 2201 3 -0.19787825125312253 2201 4 0.1329554627229328 2201 5 -0.6946877728232508 2201 6 -0.12648576867729133 2201 7 -0.457357213889658 2201 8 0.4256202958930757 2201 9 -0.06736263855040647 2201 10 0.4862586099424959 2201 11 0.40386943324158153 2201 12 -0.19032613079794203 2201 13 -0.19602948269897524 2201 14 0.1918322748480037 2201 15 0.2795476783977975 2201 16 0.3293169343901923 2201 17 0.5442777789480147 2201 18 0.09142647121326064 2201 19 0.5372471443572238 2201 20 0.1827243627511166 2201 21 0.21236422642477493 2201 22 -0.13746089691994712 2201 23 0.3326888327629973 2201 24 0.5815856744604773 2201 25 0.11057804219225886 2201 26 0.02706076300723481 2201 27 0.6403803760496203 2201 28 0.476733815778708 2201 29 0.2146809613176046 2201 30 -0.37956614157561575 2201 31 -0.4089386734266185 2201 32 -0.18229407519883978 2201 33 -0.3276952041978163 2201 34 -1.1727980729599992 2201 35 -2.4325669158287155E-4 2201 36 0.14062077769261805 2201 37 0.4193812473412848 2201 38 0.7069786995381975 2201 39 -0.08896917581813568 2201 40 0.25569881439579234 2201 41 0.8187460830155402 2201 42 -0.42856384699287275 2201 43 -0.7544254972843802 2201 44 0.060476410256981694 2201 45 -0.37301997251239527 2201 46 0.017860381437344586 2201 47 0.4228043942334452 2201 48 0.4087338970659147 2201 49 0.3143432828005004 2201 50 -0.15562963880854852 2201 51 -0.5426716423220839 1101 1 0.6544171464696831 1101 2 0.48250663395489823 1101 3 0.8551804254010591 1101 4 -0.006370379973695606 1101 5 0.05275292630283701 1101 6 0.44904481808924857 1101 7 0.20082006451234236 1101 8 -0.08199518475611 1101 9 0.38669478966432536 1101 10 0.6901287722396248 1101 11 -0.14556876900561377 1101 12 0.25636550465774377 1101 13 0.26077451230726467 1101 14 -0.08781816210580473 1101 15 -0.7353087510653751 1101 16 0.26951668470230317 1101 17 -0.050728463249235745 1101 18 0.15647016659403334 1101 19 0.24186637032476455 1101 20 0.6241725525139117 1101 21 0.15368757224250373 1101 22 0.5193430980862956 1101 23 0.7629010674696133 1101 24 0.19744085548419577 1101 25 0.2208152456878878 1101 26 0.638920937788761 1101 27 0.8894440464616054 1101 28 0.48372507670255516 1101 29 0.35750243447616015 1101 30 0.13766232284385893 1101 31 0.4971786798817419 1101 32 0.021451243153222777 1101 33 0.30051952738769927 1101 34 0.5871270206729591 1101 35 1.0473222863795755 1101 36 0.807911664084628 1101 37 -0.227809763894669 1101 38 -0.2648065688762884 1101 39 0.4819421849808663 1101 40 0.3122531215119011 1101 41 0.9128784175973124 1101 42 0.13535625288228204 1101 43 0.29101769872061567 1101 44 0.585694887160848 1101 45 0.2800387041417878 1101 46 0.6012561367432099 1101 47 0.2013312356816357 1101 48 0.6435653577400728 1101 49 0.8062284263710353 1101 50 -0.025542248046996008 1101 51 0.5419725524344727 2202 1 -0.08715588723818257 2202 2 0.2108462094854815 2202 3 0.5886386026794597 2202 4 0.33520265373417146 2202 5 0.46261559796326424 2202 6 -0.31749700463235087 2202 7 -0.13151451726044172 2202 8 0.3020247902750981 2202 9 0.5730867996518398 2202 10 0.2727754638750852 2202 11 0.19038628717416373 2202 12 0.14675252088745339 2202 13 -0.11737779730571418 2202 14 0.05700081417384695 2202 15 -0.06876692834377707 2202 16 0.22819333888457294 2202 17 0.1285474418693653 2202 18 -0.4703712815957246 2202 19 -0.2717416196877238 2202 20 0.2501400930881985 2202 21 0.3584316421551108 2202 22 -0.3734159530997232 2202 23 0.4450483833247958 2202 24 -0.2611109547530077 2202 25 0.6499038848888858 2202 26 0.3191955944679137 2202 27 0.6066725108810758 2202 28 0.49920572589106627 2202 29 0.5068157927782835 2202 30 0.6653776786491008 2202 31 0.7708366074722548 2202 32 0.6491665989584661 2202 33 -0.2715154289169206 2202 34 0.4901232753546054 2202 35 0.7413297770162828 2202 36 0.6125308900521631 2202 37 0.08230259565588938 2202 38 0.21259667706628704 2202 39 -0.4822276027844268 2202 40 0.4242381402384936 2202 41 -0.00147863608557941 2202 42 -0.06901328519511907 2202 43 0.1332149521538213 2202 44 0.5773303428412504 2202 45 0.20001373535276912 2202 46 -0.31921827024805083 2202 47 -0.1165214484631818 2202 48 -0.4564346422599286 2202 49 0.08962418167690345 2202 50 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29 -0.0246067933957832 1215 30 0.052351588380581404 1215 31 0.43823329381771003 1215 32 0.425971017164521 1215 33 0.025189960532706834 1215 34 0.5001213709554477 1215 35 0.5433674841196918 1215 36 0.29002089195656566 1215 37 0.045071592037530195 1215 38 0.15308420325177252 1215 39 0.928646516430959 1215 40 0.27778984467573764 1215 41 0.5025206021547532 1215 42 -0.4215175700932363 1215 43 -0.7238598907332445 1215 44 -0.14443693958114778 1215 45 0.12259990941485199 1215 46 -0.1629623190006697 1215 47 0.2105591076213713 1215 48 0.16220942553668038 1215 49 -0.09395989754609957 1215 50 0.5776592585443368 1215 51 -0.043338182028804795 1212 1 -0.4897269976744596 1212 2 -0.11449036304210125 1212 3 -0.6568315866109558 1212 4 -0.6135775871808988 1212 5 -0.9205203469704344 1212 6 -1.265669896625468 1212 7 -0.4556363919441182 1212 8 -0.773286476047403 1212 9 -0.09408599111645799 1212 10 -1.264525882415029 1212 11 -1.009532732969575 1212 12 -0.76285371456148 1212 13 -0.6332194816867229 1212 14 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50 -1.4477644702692203 1212 51 -0.978748168286252 1213 1 -0.8331861593154386 1213 2 -0.5793850097549296 1213 3 -1.0754899143601904 1213 4 -1.0427340163373273 1213 5 -0.13576865312783326 1213 6 -1.1221213894405935 1213 7 -1.1387575296107073 1213 8 -0.6729961030297531 1213 9 -1.7063263306295227 1213 10 -1.6860879403407196 1213 11 -1.2769737276832984 1213 12 -1.7897318323486857 1213 13 -1.1001988577570145 1213 14 0.07287972586481573 1213 15 -1.809597088990074 1213 16 -1.5403088433050414 1213 17 -0.8409947626064422 1213 18 -1.302574828814599 1213 19 -1.336278716911231 1213 20 -0.9877650048781916 1213 21 -1.4321710136160846 1213 22 -1.1573327513168294 1213 23 -1.0887059114100879 1213 24 -2.4542579507600664 1213 25 -2.282123229977863 1213 26 -1.508829291779648 1213 27 -2.142236394309748 1213 28 -0.8775237754553871 1213 29 -1.5760145535862833 1213 30 -1.1099960793600019 1213 31 -0.8831702641035619 1213 32 -1.1262071590506366 1213 33 -0.747047047451602 1213 34 -0.4985479104565158 1213 35 -1.3557080166507234 1213 36 -1.9106374635554104 1213 37 -1.323677532489711 1213 38 -1.19997644950071 1213 39 -1.8805004229162847 1213 40 -0.8197028013531167 1213 41 -1.6514925649554897 1213 42 -1.146829238394119 1213 43 -0.8215626502233704 1213 44 -0.08308380889057077 1213 45 -0.24236717280954423 1213 46 -0.8318935418610138 1213 47 -0.47672202418980447 1213 48 -0.9192445596153007 1213 49 -0.9779772669255635 1213 50 -1.4973512471287265 1213 51 -2.2304792381991376 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 0.0 15 0.0 16 0.0 17 3.0 18 1.0 19 1.0 20 5.0 21 5.0 22 15.0 23 48.0 24 84.0 25 174.0 26 262.0 27 382.0 28 529.0 29 749.0 30 890.0 31 1158.0 32 1439.0 33 1789.0 34 2112.0 35 2592.0 36 3571.0 37 5572.0 38 10198.0 39 33169.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.62833088746428 24.450451842125588 13.98316212249942 25.93805514791071 2 10.991675547112695 24.15635762714482 46.04240992138875 18.809556904353737 3 18.289085545722713 28.456037931087742 31.81207431775008 21.442802205439467 4 13.08282753401597 22.352468763996356 37.83070008803225 26.734003613955426 5 12.143816892924987 38.53959134504007 37.855410894376746 11.461180867658188 6 28.21356314383234 42.13810251895782 17.518417272853632 12.129917064356206 7 22.471389519529257 33.6236853078812 24.457520579468408 19.447404593121128 8 19.645091043877123 45.71499173732413 21.57407836414462 13.065838854654126 9 22.75247494169794 18.306074225084558 23.015027259108248 35.92642357410925 10 15.252745216142335 31.222103816275155 35.311742266289826 18.213408701292686 11 29.177735219620683 22.47482547723482 30.34379440291592 18.00364490022858 12 22.885295525799627 24.872971011135306 33.44298753648705 18.798745926578018 13 29.21280637538804 23.626619716134613 28.633646851688827 18.526927056788523 14 16.913002517413396 26.71083723300746 28.229007397797652 28.14715285178149 15 19.311495158226382 37.85232204358368 23.25595762096712 19.580225177222815 16 18.64459133872861 31.52220918020634 33.32921480200161 16.503984679063446 17 16.872847457103585 35.46464038054642 32.120959397056325 15.541552765293673 18 20.064869874121555 26.857672407135684 37.55965711637964 15.517800602363115 19 20.184095507266523 28.866855086565042 28.991953543684073 21.957095862484362 20 20.872909234119447 28.766467435790517 31.245270197223125 19.115353132866915 21 16.28442138102519 30.752598495729664 27.744057823286845 25.2189222999583 22 18.40955072665215 37.367372469072876 28.020509969265934 16.202566835009037 23 15.320699933589706 32.596642419187944 32.09315973991876 19.989497907303587 24 22.567143894114196 29.68694497212312 32.91942732706297 14.826483806699716 25 17.107600117376332 33.74569491420717 31.657631778096956 17.489073190319544 26 17.016479018980988 33.53719748567545 32.78351789216822 16.662805603175336 27 16.668983304761465 34.19975598078735 31.589677060649585 17.541583653801602 28 15.543097190690203 31.337935721014997 29.784243772104592 23.33472331619021 29 21.235849202304284 29.68694497212312 29.435203632488534 19.642002193084064 30 16.324828566133316 31.40791993575091 32.478223265583495 19.78902823253228 31 21.302259494355123 30.008185454601616 31.65145407651083 17.038100974532426 32 16.546973698435497 34.71404963783225 31.44141222258259 17.29756444114967 33 15.6419404160682 35.41367434246089 33.52793093329627 15.416454308174643 34 16.52380731748753 30.74796521954007 34.0638465458926 18.6643809170798 35 15.75468347001498 32.28312406369211 32.392778266845816 19.569414199447095 36 15.546186041483265 31.631376546355927 36.090132666141564 16.732304746019246 37 15.790205254135198 32.07308220976386 30.324792660890516 21.811919875210428 38 16.923813495189115 33.0893141206814 32.01748289548873 17.96938948864075 39 19.481381951844817 27.373395728119355 32.05918238119508 21.086039938840752 40 20.236605970748585 27.331696242413013 35.01521259015583 17.416485196682572 41 20.30456068819596 26.304653353719747 32.73872955566881 20.652056402415482 42 19.052031691609137 30.45452439419914 33.580441396778326 16.913002517413396 43 14.855827889233812 30.66147739733432 36.44226165655068 18.040433056881184 44 14.014116048124295 32.10551514309101 33.761139168172484 20.11922964061221 45 15.116835781247588 30.439080140233827 34.59358445690281 19.85049962161578 46 17.90143477119338 27.14173191863967 33.04143693338894 21.91539637677802 47 19.19411882809001 27.311618712258106 31.055305873449786 22.438956586202103 48 13.935350352901201 29.500069499142846 31.39353503529012 25.171045112665837 49 19.549336669292188 27.45679469953204 31.208203987706373 21.785664643469396 50 14.227246752845604 32.29856831765742 32.07153778436733 21.402647145129656 51 14.045438398690289 29.90099927409764 32.05708372588691 23.99647860132516 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 226.0 1 132.5 2 39.0 3 72.0 4 105.0 5 108.5 6 112.0 7 146.5 8 181.0 9 138.5 10 96.0 11 159.5 12 223.0 13 417.0 14 611.0 15 656.0 16 701.0 17 529.0 18 357.0 19 317.0 20 277.0 21 397.0 22 517.0 23 355.5 24 194.0 25 214.0 26 359.5 27 485.0 28 632.0 29 779.0 30 1711.0 31 2643.0 32 4458.5 33 6274.0 34 8690.0 35 11106.0 36 11792.0 37 12478.0 38 10558.5 39 8639.0 40 6790.0 41 4941.0 42 3723.5 43 2506.0 44 1991.5 45 1477.0 46 1387.5 47 1298.0 48 1279.0 49 1260.0 50 1918.5 51 2577.0 52 1788.5 53 1000.0 54 905.0 55 810.0 56 776.0 57 742.0 58 669.0 59 596.0 60 541.5 61 487.0 62 401.0 63 315.0 64 248.5 65 182.0 66 151.5 67 121.0 68 89.5 69 58.0 70 55.0 71 52.0 72 38.5 73 25.0 74 20.5 75 11.5 76 7.0 77 4.0 78 1.0 79 1.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02162195555143709 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0015444253965312207 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0015444253965312207 17 0.0 18 0.006177701586124883 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0015444253965312207 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0030888507930624414 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 64749.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.20835696790216 #Duplication Level Percentage of deduplicated Percentage of total 1 89.38332008633412 64.54222683770273 2 6.832629688238259 9.867459271155886 3 1.6593441805662552 3.5945555076881774 4 0.7274267273857966 2.101051551962658 5 0.3062292818896156 1.105615665035485 6 0.2281746077331582 0.9885668115724161 7 0.13610076597777235 0.6879328885329529 8 0.09152116388003088 0.5286874297253715 9 0.06980177240312158 0.45362441688091776 >10 0.48481597187036973 7.0902974168461865 >50 0.04880764223340605 2.459310243746589 >100 0.029706237395156643 4.285480670820545 >500 0.0 0.0 >1k 0.0021218740929489404 2.2951912883301184 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC 1498 2.3135492440037684 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA 466 0.7197022347835488 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 371 0.5729818221130828 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT 240 0.37066209516749293 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 224 0.3459512888229934 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 215 0.33205146025421245 No Hit TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT 196 0.30270737772011924 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 156 0.2409303618588704 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 153 0.23629708566927674 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC 151 0.23320823487621428 No Hit GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCAT 150 0.23166380947968307 No Hit GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA 126 0.19459759996293377 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 122 0.1884198983768089 No Hit CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC 118 0.182242196790684 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAG 109 0.16834236822190304 No Hit GTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAAC 96 0.14826483806699717 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 95 0.14672041267046596 No Hit GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCAT 92 0.1420871364808723 No Hit ACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCA 90 0.13899828568780986 No Hit CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT 90 0.13899828568780986 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC 90 0.13899828568780986 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 87 0.1343650094982162 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 81 0.12509845711902887 No Hit GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 73 0.1127430539467791 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 71 0.10965420315371666 No Hit GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTT 69 0.10656535236065422 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 65 0.10038765077452934 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0015444253965312205 4 0.0 0.0 0.0015444253965312205 5 0.0 0.0 0.0015444253965312205 6 0.0 0.0 0.0015444253965312205 7 0.0 0.0 0.003088850793062441 8 0.0 0.0 0.003088850793062441 9 0.0 0.0 0.010810977775718544 10 0.0 0.0 0.012355403172249764 11 0.0 0.0 0.015444253965312205 12 0.0 0.0 0.020077530154905866 13 0.0 0.0 0.02162195555143709 14 0.0 0.0 0.02316638094796831 15 0.0 0.0 0.02316638094796831 16 0.0 0.0 0.03088850793062441 17 0.0 0.0 0.03552178412021807 18 0.0 0.0 0.04015506030981173 19 0.0 0.0 0.04324391110287418 20 0.0 0.0 0.05405488887859272 21 0.0 0.0 0.07567684443002981 22 0.0 0.0 0.12355403172249764 23 0.0 0.0 0.20540857773865234 24 0.0 0.0 0.287263123754807 25 0.0 0.0 0.32124048247849385 26 0.0 0.0 0.420083707856492 27 0.0 0.0 0.45251664118364765 28 0.0 0.0 0.5621708443373643 29 0.0 0.0 0.6841804506633307 30 0.0 0.0 0.7907458030239849 31 0.0 0.0 1.3282058410168496 32 0.0 0.0 1.8054332885449969 33 0.0 0.0 2.2733941836939566 34 0.0 0.0 2.977652164512193 35 0.0 0.0 3.54291185964262 36 0.0 0.0 4.571499173732413 37 0.0 0.0 5.593908786236081 38 0.0 0.0 6.761494386013684 39 0.0 0.0 8.091244652427065 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATATAA 25 3.8499995E-5 45.0 30 AGTGGTC 20 6.983987E-4 45.0 8 ATCTCGT 230 0.0 40.1087 37 TGCCGTC 230 0.0 40.1087 45 TCTCGTA 230 0.0 40.1087 38 CTCGTAT 230 0.0 40.1087 39 CGTATGC 240 0.0 39.375004 41 TCGTATG 240 0.0 39.375004 40 TATGCCG 235 0.0 39.255318 43 ATGCCGT 235 0.0 39.255318 44 GTATGCC 245 0.0 38.57143 42 CGAGCCC 245 0.0 38.57143 15 AATCTCG 35 6.1573846E-6 38.571426 36 CGAGACA 35 6.1573846E-6 38.571426 23 CACGAGA 240 0.0 38.437504 21 ACGAGAC 240 0.0 38.437504 22 CGATAAC 200 0.0 38.25 28 AACCTAT 200 0.0 38.25 32 AGACGAT 195 0.0 38.076927 25 GACGATA 195 0.0 38.076927 26 >>END_MODULE