FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_56.34100000006b96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_56.34100000006b96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64749
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC14982.3135492440037684No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA4660.7197022347835488No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.5729818221130828No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT2400.37066209516749293No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2240.3459512888229934No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2150.33205146025421245No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT1960.30270737772011924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.2409303618588704No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.23629708566927674No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC1510.23320823487621428No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCAT1500.23166380947968307No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA1260.19459759996293377No Hit
GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.1884198983768089No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC1180.182242196790684No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAG1090.16834236822190304No Hit
GTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAAC960.14826483806699717No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.14672041267046596No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCAT920.1420871364808723No Hit
ACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCA900.13899828568780986No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT900.13899828568780986No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC900.13899828568780986No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.1343650094982162No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.12509845711902887No Hit
GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.1127430539467791No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.10965420315371666No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTT690.10656535236065422No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.10038765077452934No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAA253.8499995E-545.030
AGTGGTC206.983987E-445.08
ATCTCGT2300.040.108737
TGCCGTC2300.040.108745
TCTCGTA2300.040.108738
CTCGTAT2300.040.108739
CGTATGC2400.039.37500441
TCGTATG2400.039.37500440
TATGCCG2350.039.25531843
ATGCCGT2350.039.25531844
GTATGCC2450.038.5714342
CGAGCCC2450.038.5714315
AATCTCG356.1573846E-638.57142636
CGAGACA356.1573846E-638.57142623
CACGAGA2400.038.43750421
ACGAGAC2400.038.43750422
CGATAAC2000.038.2528
AACCTAT2000.038.2532
AGACGAT1950.038.07692725
GACGATA1950.038.07692726
Produced by FastQC (version 0.11.2)