FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_55.34100000006b89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_55.34100000006b89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62445
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC15392.4645688205620946No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC1010.16174233325326287No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA890.14252542237168708No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT790.12651132997037393No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC720.11530146528945472No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG710.1137000560493234No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2450.043.16326545
ATGCCGT2500.042.344
ATCTCGT2700.039.16666437
TATGCCG2700.039.16666443
TAACCTA2700.039.16666431
TCTCGTA2700.039.16666438
ACCTATC2750.038.45454433
TATCTCG2750.038.45454436
GACGATA2750.038.45454426
CCTATCT2750.038.45454434
CTCGTAT2800.037.76785739
TCGTATG2800.037.76785740
CGAGACG2800.037.76785723
CGATAAC2800.037.76785728
CGTATGC2850.037.1052641
AGACGAT2850.037.1052625
ACGATAA2850.037.1052627
GAGACGA2850.037.1052624
AACCTAT2850.037.1052632
ATAACCT2850.037.1052630