FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_54.34100000006b7c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_54.34100000006b7c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82080
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC8471.0319200779727096No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA5160.6286549707602339No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT2870.3496588693957115No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC2360.2875243664717349No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAG1640.19980506822612085No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACA1250.15229044834307992No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTT1180.14376218323586745No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT1070.13036062378167643No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCAT1020.12426900584795322No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATT980.11939571150097465No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA960.11695906432748539No Hit
ACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCA920.11208576998050682No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACAC920.11208576998050682No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT890.1084307992202729No Hit
TTTTTCAAGTCGTCAAGTGGATGTTTCTCATTTTCCATGATTTTCAGTTTT890.1084307992202729No Hit
GTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAAC870.10599415204678361No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG850.039.7053443
ATGCCGT850.039.7053444
ACGAGAC850.039.7053422
CCACGAG850.039.7053420
TGCCGTC850.039.7053445
CGAGACG750.038.99947423
GAGACGA700.038.57090824
GCCCACG900.037.49949318
CTCGTAT900.037.49949339
CACGAGA900.037.49949321
CCCACGA900.037.49949319
GAAAGAT250.002095888635.9995119
ATCTGAT250.002095888635.9995113
ATCTCGT950.035.52583737
GTATGCC950.035.52583742
TCTCGTA950.035.52583738
CGAGCCC950.035.52583715
AGACGAT653.5652192E-1034.61491425
TAACCTA653.5652192E-1034.61491431
GACGATA653.5652192E-1034.61491426