FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_53.34100000006b6f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_53.34100000006b6f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64015
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC25644.005311255174568No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC3140.49051003671014604No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA3100.48426150121065376No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2580.4030305397172538No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT2270.3546043895961884No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC2150.3358587830977115No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT2040.31867531047410763No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC1530.2390064828555807No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1460.22807154573146918No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC1340.20932593923299225No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAG1300.20307740373349997No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACACA1290.2015152698586269No Hit
TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA1180.18433179723502305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.18433179723502305No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC1130.17652112786065766No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT990.15465125361243456No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.15152698586268842No Hit
TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC930.14527845036319612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.13902991486370384No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAACTGTCTCTTATACACA860.13434351323908458No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCAT830.12965711161446536No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAA820.12809497773959228No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACACATC810.12653284386471922No Hit
CAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAG800.12497070998984613No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCGAG760.11872217449035381No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCC700.10934937124111535No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACA690.1077872373662423No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCAT670.10466296961649613No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA670.10466296961649613No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA660.10310083574162307No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC660.10310083574162307No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAG206.9834164E-445.03
CTAAGCA206.9834164E-445.04
TGCCGTC3750.040.845
GCCGTCT403.3905053E-739.37545
ATGCCGT3950.038.73417744
CGAGACG3900.038.65384723
TAACCTA3750.038.39999831
AGACGAT3750.038.39999825
GACGATA3750.038.39999826
CACGAGA4050.038.33333221
ACGAGAC4050.038.33333222
GTATGCC4000.038.2542
CGTATGC4000.038.2541
TCGTATG4000.038.2540
GAGACGA3900.038.07692324
CGATAAC3800.037.89473728
GATAACC3800.037.89473729
AACCTAT3800.037.89473732
ACGATAA3800.037.89473727
CCACGAG4100.037.86585220
Produced by FastQC (version 0.11.2)