FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_52.34100000006b53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_52.34100000006b53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109373
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC5820.5321240159820065No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA1910.17463176469512584No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA1360.12434513088239328No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT1170.10697338465617658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT950.040.26315739
TATGCCG950.040.26315743
TCTCGTA950.040.26315738
CGAGCCC950.040.26315715
ATGCCGT950.040.26315744
TGCCGTC950.040.26315745
TCCGAGC1050.038.5714313
ACGATAA700.038.5714327
GACGATA700.038.5714326
CGATAAC700.038.5714328
ATCTCGT1000.038.25000437
ATCTCCG1000.038.25000410
TATCTCG1000.038.25000436
TCGTATG1000.038.25000440
TCTCCGA1000.038.25000411
ACGAGAC1000.038.25000422
CGAGACG900.037.523
ACCTATC800.036.562533
AACCTAT800.036.562532
CTCCGAG1050.036.4285712