FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_51.34100000006b46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_51.34100000006b46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84274
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC5450.646700049837435No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA1960.23257469682227022No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT1430.1696846002325747No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA1160.13764624913971094No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC1130.13408643235161496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGA702.0008883E-1135.35014719
CGATAAC653.5652192E-1034.62908628
CTCGTAT653.583409E-1034.60853639
TCTCGTA653.583409E-1034.60853638
CCTATCT653.583409E-1034.60853634
AGACGAT707.876224E-1032.15558225
ACGATAA707.876224E-1032.15558227
GACGATA707.876224E-1032.15558226
CTATCTC707.930794E-1032.13649735
TATGCCG707.930794E-1032.13649743
CGAGCCC707.930794E-1032.13649715
AACCTAT707.930794E-1032.13649732
CGAGACG707.930794E-1032.13649723
ATGCCGT707.930794E-1032.13649744
TGCCGTC707.930794E-1032.13649745
GCCCACG809.640644E-1130.93138118
GAGACGA751.6480044E-930.01187724
TCTCCGA751.6480044E-930.01187711
AGCCCAC751.6589183E-929.99406417
ACCTATC751.6589183E-929.99406433