FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_50.34100000006b39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_50.34100000006b39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12226
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC6745.51284148535907No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.6298053328971045No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT620.5071159823327336No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.2862751513168657No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA170.13904793063962048No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.13086864060199577No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA160.13086864060199577No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC140.11451006052674627No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.11451006052674627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.10633077048912154No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG1100.038.86363610
CATCTCC1100.038.8636369
CGAGCCC1100.038.86363615
CCGAGCC1100.038.86363614
GCCCACG1050.038.5714318
TGCCGTC1050.038.5714345
ATGCCGT1050.038.5714344
CGATAAC950.037.89473728
AACCTAT950.037.89473732
AGACGAT950.037.89473725
GACGATA950.037.89473726
ACCTATC950.037.89473733
ACGATAA950.037.89473727
CGAGACG950.037.89473723
GAGACGA950.037.89473724
ACATCTC1150.037.1739128
TCTCCGA1150.037.17391211
TCCGAGC1150.037.17391213
CTCCGAG1150.037.17391212
AGCCCAC1100.036.8181817