FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_5.34100000006862.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_5.34100000006862.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1740
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC62736.03448275862069RNA PCR Primer, Index 27 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT673.8505747126436782RNA PCR Primer, Index 38 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC281.6091954022988506No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC140.8045977011494253No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC100.5747126436781609No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT80.45977011494252873No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCG70.40229885057471265No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC70.40229885057471265TruSeq Adapter, Index 12 (95% over 21bp)
GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAGTACTCTGCGTTGATA70.40229885057471265No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC70.40229885057471265No Hit
ATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCTTC60.3448275862068966RNA PCR Primer, Index 27 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC60.3448275862068966No Hit
TATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCTT50.28735632183908044RNA PCR Primer, Index 27 (95% over 23bp)
GATACCACTGCTTGGAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCA50.28735632183908044No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAGTACTC40.22988505747126436No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC40.22988505747126436No Hit
TGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATAC30.1724137931034483No Hit
GATACCACTGCTTGGAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG30.1724137931034483No Hit
GATACCACTGCTTGGAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT30.1724137931034483No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA30.1724137931034483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTCCAAGCAGTGGTATCAACG20.11494252873563218No Hit
GTGGTATCAACGCAGAGTACTTTTTTTGCAGTGGCATCAACGCAAAAAAAA20.11494252873563218No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTCCAAGCAGTGGTAT20.11494252873563218No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTCCAAGCAGTGGTATCAAC20.11494252873563218No Hit
GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAGTACTCTGCGTTGAT20.11494252873563218No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTCCAAGCAGTGGTATCAACGC20.11494252873563218No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTCCAAGCAGTGGTATC20.11494252873563218No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCAAGCAGTGGTATCAA20.11494252873563218No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAG20.11494252873563218No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAA20.11494252873563218No Hit
GATACCACTGCTTGGAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACC20.11494252873563218No Hit
ATACCACTGCTTGGAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT20.11494252873563218No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11494252873563218No Hit
GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAGTACTCTGCGTTG20.11494252873563218No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC20.11494252873563218No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG20.11494252873563218No Hit
GAGTACTTTTTTTGCAGTGGCATCAACGCAAAAAAAAGTACTCTGCGTTGA20.11494252873563218No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTGA20.11494252873563218No Hit
GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAGTACTCTGCGTTG20.11494252873563218No Hit
TGATACCACTGCTTGGAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACC20.11494252873563218No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTCCAAGCAGTGGTATCAA20.11494252873563218No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAA20.11494252873563218No Hit
ATACCACTGCTTGGAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC20.11494252873563218No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATATCGTATGCCGTC20.11494252873563218No Hit
GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGA20.11494252873563218No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAGTA20.11494252873563218No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTCCAAGCAGT20.11494252873563218No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA205.2271195E-445.01
CTTATAC700.045.01
AGCCCAC850.037.05882317
GTATGCC850.037.05882342
ATCTCGT850.037.05882337
ATCTCCG850.037.05882310
CACATCT850.037.0588237
GCCCACG850.037.05882318
TATACAC850.037.0588233
TGCCGTC850.037.05882345
CCACGAG850.037.05882320
TATGCCG850.037.05882343
GAGCCCA850.037.05882316
CACGAGA850.037.05882321
CCCACGA850.037.05882319
ACATCTC850.037.0588238
TTATACA850.037.0588232
TACACAT850.037.0588235
ACACATC850.037.0588236
TCTCGTA850.037.05882338
Produced by FastQC (version 0.11.2)