##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_49.34100000006b2c.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 72547 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.33625098212193 38.0 38.0 38.0 32.0 38.0 2 36.34868430121163 38.0 38.0 38.0 32.0 38.0 3 36.33631990295946 38.0 38.0 38.0 32.0 38.0 4 36.43990792176106 38.0 38.0 38.0 32.0 38.0 5 36.44992901153735 38.0 38.0 38.0 32.0 38.0 6 38.364673935517665 40.0 38.0 40.0 38.0 40.0 7 38.24669524584063 40.0 38.0 40.0 38.0 40.0 8 38.24029939211821 40.0 38.0 40.0 38.0 40.0 9 38.27864694611769 40.0 38.0 40.0 38.0 40.0 10 38.20588032585772 40.0 38.0 40.0 38.0 40.0 11 38.20991908693674 40.0 38.0 40.0 38.0 40.0 12 38.12065281817305 40.0 38.0 40.0 38.0 40.0 13 38.16086123478573 40.0 38.0 40.0 38.0 40.0 14 38.09136146222449 40.0 38.0 40.0 38.0 40.0 15 38.10390505465423 40.0 38.0 40.0 38.0 40.0 16 38.112037713482295 40.0 38.0 40.0 38.0 40.0 17 38.09822597764208 40.0 38.0 40.0 38.0 40.0 18 38.11558024453113 40.0 38.0 40.0 38.0 40.0 19 38.14317614787655 40.0 38.0 40.0 38.0 40.0 20 38.152935338470236 40.0 38.0 40.0 38.0 40.0 21 38.16721573600562 40.0 38.0 40.0 38.0 40.0 22 38.153610762677985 40.0 38.0 40.0 38.0 40.0 23 38.13389940314555 40.0 38.0 40.0 38.0 40.0 24 38.12416778088687 40.0 38.0 40.0 38.0 40.0 25 38.10908790163618 40.0 38.0 40.0 38.0 40.0 26 38.078280287262054 40.0 38.0 40.0 38.0 40.0 27 38.059382193612414 40.0 38.0 40.0 38.0 40.0 28 38.04060815747033 40.0 38.0 40.0 38.0 40.0 29 37.99658152645871 40.0 38.0 40.0 32.0 40.0 30 37.995092836368144 40.0 38.0 40.0 32.0 40.0 31 37.98876590348326 40.0 38.0 40.0 32.0 40.0 32 37.94865397604312 40.0 38.0 40.0 32.0 40.0 33 37.93207162253436 40.0 38.0 40.0 32.0 40.0 34 37.940576453885065 40.0 38.0 40.0 32.0 40.0 35 37.942657863178354 40.0 38.0 40.0 32.0 40.0 36 37.90942423532331 40.0 38.0 40.0 32.0 40.0 37 37.92515197044675 40.0 38.0 40.0 32.0 40.0 38 37.929755882393486 40.0 38.0 40.0 32.0 40.0 39 37.851530731801454 40.0 38.0 40.0 32.0 40.0 40 37.84326023129833 40.0 38.0 40.0 32.0 40.0 41 37.841206390340055 40.0 38.0 40.0 32.0 40.0 42 37.7957737742429 40.0 38.0 40.0 32.0 40.0 43 37.798323845231366 40.0 38.0 40.0 32.0 40.0 44 37.74500668532124 40.0 38.0 40.0 32.0 40.0 45 37.74704674211201 40.0 38.0 40.0 32.0 40.0 46 37.662687636980166 40.0 38.0 40.0 32.0 40.0 47 37.66380415454809 40.0 38.0 40.0 32.0 40.0 48 37.59693715798034 40.0 38.0 40.0 32.0 40.0 49 37.547727679986764 40.0 38.0 40.0 32.0 40.0 50 37.499648503728615 40.0 38.0 40.0 32.0 40.0 51 37.04328228596634 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.04643140418263414 2201 2 0.15481873292507942 2201 3 0.8683455389250128 2201 4 0.8697033140366344 2201 5 0.6308029986328094 2201 6 0.40564082197190743 2201 7 0.6085287266060746 2201 8 0.5160616470538244 2201 9 0.9340298744268978 2201 10 0.31933336680877744 2201 11 0.762784076219134 2201 12 1.0276523535812316 2201 13 -0.10972958919138875 2201 14 0.6234484487960188 2201 15 0.6856741169289933 2201 16 0.631870343651002 2201 17 0.7911757012436595 2201 18 -0.016822756317829146 2201 19 0.8263566796508144 2201 20 0.4095288356041138 2201 21 -0.23398646742943896 2201 22 0.7669300231056013 2201 23 0.1402871491301525 2201 24 0.31294632079597307 2201 25 0.05334473282595553 2201 26 0.5343958595596305 2201 27 0.8338166465633421 2201 28 0.6098975457433298 2201 29 0.1585588672727951 2201 30 0.7272049515331389 2201 31 0.899918715047761 2201 32 0.5785800497637013 2201 33 0.7408973825549552 2201 34 0.6536033705629407 2201 35 0.37348125780684427 2201 36 0.20856431602292247 2201 37 0.4248712951027329 2201 38 0.9861952574307793 2201 39 1.0742453177934053 2201 40 0.8647599291178452 2201 41 0.6622819689868962 2201 42 0.3780672921132293 2201 43 0.21481024548403838 2201 44 0.24194396753107128 2201 45 -0.011378444477557537 2201 46 0.09999881619364004 2201 47 0.16751931034890788 2201 48 0.174788313134151 2201 49 -0.34988580864295926 2201 50 0.5751066587990508 2201 51 0.33336508466808823 1101 1 -0.5527806105318405 1101 2 -0.07825005014370134 1101 3 -0.9155168949374186 1101 4 -0.06151361718029591 1101 5 -0.43427636644656076 1101 6 -0.19065547432438734 1101 7 0.4627615308388826 1101 8 0.21447434546652033 1101 9 0.38164892204594025 1101 10 0.356105853581262 1101 11 0.22918619262124906 1101 12 0.14722907315795197 1101 13 0.207995278533474 1101 14 0.12106749641506553 1101 15 0.20286988412475893 1101 16 0.3202301320107921 1101 17 -0.4413639812960284 1101 18 -0.13639947589454238 1101 19 0.006250859544998377 1101 20 -0.01163518555991061 1101 21 0.47024633680336336 1101 22 0.2788347850103676 1101 23 -0.11738480854180722 1101 24 -0.055572197722540295 1101 25 0.21895319843442707 1101 26 0.09550697067074765 1101 27 0.16318172592841762 1101 28 0.41042664627243397 1101 29 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0.4590340343816024 2202 15 -0.17293449167961938 2202 16 -0.24450603272537563 2202 17 0.029163689231644696 2202 18 0.8170110775160069 2202 19 0.25078110407523724 2202 20 0.03315245922773613 2202 21 -0.05330578674875852 2202 22 -0.13171862554304425 2202 23 -0.1294826206396209 2202 24 -0.09521183736218575 2202 25 0.0047961842774100205 2202 26 -0.6270155518517768 2202 27 0.33306589581258805 2202 28 0.26705470290048794 2202 29 -0.008608299894369509 2202 30 0.06679454112272509 2202 31 0.09861741374646016 2202 32 -0.2297282585446112 2202 33 -0.10469821304064197 2202 34 -0.3181183511587875 2202 35 0.264622398947985 2202 36 0.32217792963653835 2202 37 -0.7570606368291948 2202 38 -0.4702611990256713 2202 39 -0.4845634910154004 2202 40 -0.09419902984111417 2202 41 -0.2538841971792749 2202 42 -0.05962039557445564 2202 43 -0.11927383860004426 2202 44 -0.16371168812458592 2202 45 0.6778107447116284 2202 46 -0.047899081704258606 2202 47 -0.3164646736350747 2202 48 -0.33522169687586256 2202 49 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-0.33037606698538013 1213 31 -0.59718776643372 1213 32 -1.440806524310375 1213 33 -1.2388479878154186 1213 34 -0.4745794998074331 1213 35 -1.30012985330427 1213 36 -0.7726278136067037 1213 37 -1.2232768530454123 1213 38 -0.8401936314581064 1213 39 -0.7787639414658543 1213 40 -1.4266173856969715 1213 41 -1.4337828458279205 1213 42 -1.1679396523312136 1213 43 -1.2895300322937402 1213 44 -0.5876277917281882 1213 45 -0.36814927781089324 1213 46 -0.5571192393619171 1213 47 -0.028950597058496896 1213 48 -0.8362070572362228 1213 49 -0.6383696049392569 1213 50 -0.18878223008983497 1213 51 -1.0164613042207975 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 2.0 17 1.0 18 1.0 19 2.0 20 3.0 21 7.0 22 17.0 23 47.0 24 93.0 25 176.0 26 295.0 27 346.0 28 580.0 29 805.0 30 1008.0 31 1297.0 32 1549.0 33 1748.0 34 2197.0 35 2799.0 36 3994.0 37 6004.0 38 11204.0 39 38371.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.69910918668468 19.717863151217628 13.465622328249538 27.11740533384815 2 15.047626924720511 20.982038239389052 40.007995257984916 23.962339577905517 3 19.475650268102058 23.24148483052366 29.14248693950129 28.140377961872993 4 13.861358843232663 17.550002067625126 36.15449294939832 32.43414613974389 5 14.964092243648944 36.19860228541497 34.69474961059727 14.142555860338815 6 32.45895764125326 36.488069803024246 17.25088563276221 13.802086922960289 7 28.450521730740068 32.02062111458778 20.750685762333383 18.77817139233876 8 24.29873047817277 37.698319709981114 20.115235640343503 17.887714171502612 9 24.976911519428786 17.68646532592664 21.073235281955146 36.26338787268943 10 15.913821384757467 29.205894110025227 32.58577198230113 22.29451252291618 11 33.433498283871145 23.892097536769267 24.36902973244931 18.305374446910278 12 23.532330764883454 24.92177484940797 30.034322577087956 21.51157180862062 13 27.82786094340143 22.239682408402945 26.357069996967443 23.575386651228186 14 20.681764924807368 22.949260479413347 27.091402814727005 29.27757178105228 15 22.276593105159414 31.576770920920232 23.415165341089224 22.73147063283113 16 22.4773930304367 29.18918173797971 26.61694971327746 21.71647551830613 17 21.413704219333674 29.893724068534883 27.878478779274126 20.81409293285732 18 21.921565924598525 28.02122820318423 29.32524639878696 20.731959473430283 19 22.516437619749954 27.646904765186708 27.984616869064194 21.852040745999147 20 22.52195128675204 28.039753539085005 28.178973630887562 21.259321543275394 21 21.90442058251892 27.437385419107613 28.002536286820956 22.65565771155251 22 22.23110535239224 28.642121659062404 27.761313355479896 21.365459633065463 23 21.40405530208003 28.238245551159935 28.145891628875074 22.211807517884957 24 22.559168539016085 27.3147063283113 28.78823383461756 21.33789129805505 25 21.87271699725695 27.984616869064194 28.496009483507244 21.646656650171614 26 21.4043503432305 28.291015355774267 28.54602596972955 21.75860833126568 27 21.679738652184103 28.631094325058235 27.990130536066278 21.699036486691387 28 21.83687816174342 27.999779453319917 27.2871379933009 22.876204391635767 29 22.475085117234343 27.983238452313675 27.54076667539664 22.000909755055343 30 21.775572098152743 27.99283154121864 28.03970223325062 22.191894127378 31 22.592250541028573 27.26921857554413 28.05078087308917 22.087750010338127 32 21.75141632321116 28.227218217155777 27.97772478531159 22.043640674321473 33 21.798282492728852 27.93223703254442 28.651770576316043 21.617709898410688 34 22.210429101134437 27.40430341709513 28.655905826567608 21.729361655202833 35 21.956800419038693 27.571091843908086 28.019077287827205 22.45303044922602 36 21.845148662246544 27.281624326298815 28.793747501619638 22.079479509835004 37 21.75830840696376 27.719960852964288 27.54214509214716 22.979585647924793 38 21.380622217321186 27.97634636856107 28.4932526500062 22.14977876411154 39 22.45303044922602 26.851558300136464 28.067321874095413 22.628089376542103 40 23.149130908238796 26.26159593091375 28.657284243318127 21.931988917529328 41 22.822446138365475 25.98866941431072 28.67244682757385 22.516437619749954 42 22.45992253297862 26.796421630115645 28.624202241305635 22.119453595600095 43 21.605304147656 26.86258563414063 28.855776255393057 22.676333962810315 44 21.157318703736887 27.19478407101603 28.53046990227025 23.11742732297683 45 21.387514301073786 26.8405309661323 28.620066991054077 23.151887741739838 46 21.890636415013713 26.25056859690959 28.387114560216137 23.47168042786056 47 21.82585082773926 26.490413111500132 28.450521730740068 23.233214330020537 48 20.913338938894785 26.815719464622934 28.216190883151608 24.05475071333067 49 22.003666588556385 26.16097150812577 28.666933160571766 23.16842874274608 50 21.20832012350614 27.33951782982067 28.231353467407338 23.220808579265857 51 20.50341861490957 26.929863255403617 28.473753859726507 24.092964269960298 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 105.0 1 56.5 2 8.0 3 10.5 4 13.0 5 10.0 6 7.0 7 8.5 8 10.0 9 14.5 10 19.0 11 41.5 12 64.0 13 90.5 14 117.0 15 150.0 16 183.0 17 215.5 18 248.0 19 229.0 20 210.0 21 216.5 22 223.0 23 209.0 24 195.0 25 217.5 26 361.5 27 483.0 28 834.5 29 1186.0 30 1781.5 31 2377.0 32 3078.5 33 3780.0 34 4336.0 35 4892.0 36 5013.0 37 5134.0 38 5121.5 39 5109.0 40 5009.0 41 4909.0 42 4850.5 43 4792.0 44 4899.0 45 5006.0 46 4983.5 47 4961.0 48 4855.0 49 4749.0 50 4830.0 51 4911.0 52 4570.5 53 4230.0 54 4013.0 55 3796.0 56 3411.5 57 3027.0 58 2675.0 59 2323.0 60 2077.0 61 1831.0 62 1586.0 63 1341.0 64 1101.0 65 861.0 66 681.5 67 502.0 68 413.0 69 324.0 70 250.0 71 176.0 72 136.5 73 97.0 74 79.5 75 48.0 76 34.0 77 20.5 78 7.0 79 5.0 80 3.0 81 2.0 82 1.0 83 0.5 84 0.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03997408576509022 2 0.00551366700208141 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0013784167505203524 14 0.0 15 0.0 16 0.004135250251561057 17 0.0 18 0.002756833501040705 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0013784167505203524 27 0.0 28 0.0 29 0.0 30 0.009648917253642466 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.004135250251561057 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 72547.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.37382707081493 #Duplication Level Percentage of deduplicated Percentage of total 1 93.76533763065052 85.67697745899636 2 5.052718139762816 9.233723870805143 3 0.7208990120893055 1.976139049985086 4 0.22223399722572915 0.8122548332703896 5 0.10054873550377488 0.4593761385055518 6 0.04712309381530224 0.25834904551927296 7 0.029248260600193542 0.187076785434295 8 0.01468629023826275 0.10735540356342542 9 0.011432309719014353 0.09401525021566928 >10 0.029756652254695502 0.5737916331677811 >50 0.003009877514801634 0.16498915989623505 >100 0.0030060006255789636 0.4559513706408259 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC 230 0.31703585261968104 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 102 0.14059850855307593 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0013784167505203524 6 0.0 0.0 0.0013784167505203524 7 0.0 0.0 0.0013784167505203524 8 0.0 0.0 0.0013784167505203524 9 0.0 0.0 0.0013784167505203524 10 0.0 0.0 0.0013784167505203524 11 0.0 0.0 0.0013784167505203524 12 0.0 0.0 0.0013784167505203524 13 0.0 0.0 0.0013784167505203524 14 0.0 0.0 0.0013784167505203524 15 0.0 0.0 0.0013784167505203524 16 0.0 0.0 0.0013784167505203524 17 0.0 0.0 0.0013784167505203524 18 0.0 0.0 0.0013784167505203524 19 0.0 0.0 0.0013784167505203524 20 0.0 0.0 0.0013784167505203524 21 0.0 0.0 0.0013784167505203524 22 0.0 0.0 0.0013784167505203524 23 0.0 0.0 0.004135250251561057 24 0.0 0.0 0.01102733400416282 25 0.0 0.0 0.01240575075468317 26 0.0 0.0 0.013784167505203523 27 0.0 0.0 0.019297834507284933 28 0.0 0.0 0.03583883551352916 29 0.0 0.0 0.05513667002081409 30 0.0 0.0 0.10062442278798572 31 0.0 0.0 0.24949343184418377 32 0.0 0.0 0.410768191655065 33 0.0 0.0 0.5775566184680276 34 0.0 0.0 0.7691565467903566 35 0.0 0.0 0.9800543096199705 36 0.0 0.0 1.2433319089693577 37 0.0 0.0 1.5975850138530883 38 0.0 0.0 2.018002122761796 39 0.0 0.0 2.5211242367017244 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGTAT 35 6.1741885E-6 38.565517 39 TATGCCG 35 6.1741885E-6 38.565517 43 TGCCGTC 35 6.1741885E-6 38.565517 45 TATCTCG 30 1.1304259E-4 37.49425 36 CGAGACG 30 1.1304259E-4 37.49425 23 AGACGAT 25 0.0020886236 36.019325 25 GACGATA 25 0.0020886236 36.019325 26 CGATAAC 25 0.0020886236 36.019325 28 ACCTATC 25 0.0020957172 35.994484 33 ATCTCGT 40 1.5421872E-5 33.744827 37 CGTATGC 40 1.5421872E-5 33.744827 41 TCTCGTA 40 1.5421872E-5 33.744827 38 GAGCCCA 40 1.5421872E-5 33.744827 16 CGAGCCC 40 1.5421872E-5 33.744827 15 TCTCCGA 40 1.5421872E-5 33.744827 11 ATGCCGT 40 1.5421872E-5 33.744827 44 CCACGAG 40 1.5421872E-5 33.744827 20 CTTCTAT 30 0.0050680605 30.057589 1 ACGATAA 30 0.005102451 30.016104 27 CACGAGA 45 3.450013E-5 29.995403 21 >>END_MODULE