FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_49.34100000006b2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_49.34100000006b2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72547
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC2300.31703585261968104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1020.14059850855307593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT356.1741885E-638.56551739
TATGCCG356.1741885E-638.56551743
TGCCGTC356.1741885E-638.56551745
TATCTCG301.1304259E-437.4942536
CGAGACG301.1304259E-437.4942523
AGACGAT250.002088623636.01932525
GACGATA250.002088623636.01932526
CGATAAC250.002088623636.01932528
ACCTATC250.002095717235.99448433
ATCTCGT401.5421872E-533.74482737
CGTATGC401.5421872E-533.74482741
TCTCGTA401.5421872E-533.74482738
GAGCCCA401.5421872E-533.74482716
CGAGCCC401.5421872E-533.74482715
TCTCCGA401.5421872E-533.74482711
ATGCCGT401.5421872E-533.74482744
CCACGAG401.5421872E-533.74482720
CTTCTAT300.005068060530.0575891
ACGATAA300.00510245130.01610427
CACGAGA453.450013E-529.99540321
Produced by FastQC (version 0.11.2)