H23NVBCXX l02n01 48h_48.34100000006b1f.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 48h_48.34100000006b1f.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4166
Sequences flagged as poor quality	0
Sequence length	51
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC	3204	76.90830532885262	RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC	162	3.8886221795487277	No Hit
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT	101	2.42438790206433	RNA PCR Primer, Index 18 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT	34	0.8161305808929429	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	33	0.7921267402784445	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	16	0.3840614498319731	No Hit
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACC	13	0.3120499279884782	No Hit
GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGA	11	0.2640422467594815	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC	11	0.2640422467594815	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	9	0.21603456553048486	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	9	0.21603456553048486	RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC	8	0.19203072491598655	No Hit
CGGAGATGTGTATAAGAGACAGGCGTCAGATGTGTATAAGAGACAGCTTAT	8	0.19203072491598655	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG	7	0.16802688430148824	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC	6	0.14402304368698993	No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATATCGTATGCCGTC	6	0.14402304368698993	No Hit
CCTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC	5	0.1200192030724916	RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTACGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC	5	0.1200192030724916	No Hit
CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAGA	5	0.1200192030724916	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
ACCATTC	335	43.656715	27
CATTCCG	335	43.656715	29
CCATTCC	335	43.656715	28
AGACCAT	335	43.656715	25
GACCATT	335	43.656715	26
CTTATAC	390	43.26923	1
TGCCGTC	380	43.223682	45
ATGCCGT	380	43.223682	44
GAGACCA	345	43.04348	24
TTCCGAA	335	42.985077	31
ATTCCGA	335	42.985077	30
TCCGAAT	335	42.985077	32
CCGAATC	330	42.954544	33
GTATGCC	385	42.66234	42
TATGCCG	385	42.66234	43
CGTATGC	385	42.66234	41
TCTCCGA	385	42.66234	11
TCCGAGC	385	42.66234	13
CTCCGAG	385	42.66234	12
CGAGACC	350	42.42857	23

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content