##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_47.34100000006b02.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 56889 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.180984021515584 38.0 38.0 38.0 32.0 38.0 2 36.2733744660655 38.0 38.0 38.0 32.0 38.0 3 36.22204644131554 38.0 38.0 38.0 32.0 38.0 4 36.36340944646592 38.0 38.0 38.0 32.0 38.0 5 36.38631369860606 38.0 38.0 38.0 32.0 38.0 6 38.29017912074391 40.0 38.0 40.0 38.0 40.0 7 38.13673996730475 40.0 38.0 40.0 38.0 40.0 8 38.11023220657772 40.0 38.0 40.0 38.0 40.0 9 38.178347307915416 40.0 38.0 40.0 38.0 40.0 10 38.096521295856846 40.0 38.0 40.0 38.0 40.0 11 38.11866969009826 40.0 38.0 40.0 38.0 40.0 12 37.96508991193377 40.0 38.0 40.0 32.0 40.0 13 38.065548700100194 40.0 38.0 40.0 38.0 40.0 14 37.94863681906871 40.0 38.0 40.0 32.0 40.0 15 38.008068343616515 40.0 38.0 40.0 32.0 40.0 16 37.945613387473855 40.0 38.0 40.0 32.0 40.0 17 37.93838879220939 40.0 38.0 40.0 32.0 40.0 18 37.923007962875076 40.0 38.0 40.0 32.0 40.0 19 37.989365255145984 40.0 38.0 40.0 32.0 40.0 20 37.990015644500694 40.0 38.0 40.0 32.0 40.0 21 38.03789836347976 40.0 38.0 40.0 32.0 40.0 22 38.02448628030023 40.0 38.0 40.0 32.0 40.0 23 38.04009562481323 40.0 38.0 40.0 32.0 40.0 24 37.999015626922606 40.0 38.0 40.0 32.0 40.0 25 37.98423245267099 40.0 38.0 40.0 32.0 40.0 26 37.903988468772525 40.0 38.0 40.0 32.0 40.0 27 37.903830265956515 40.0 38.0 40.0 32.0 40.0 28 37.87487915062666 40.0 38.0 40.0 32.0 40.0 29 37.871820562850466 40.0 38.0 40.0 32.0 40.0 30 37.860201444919056 40.0 38.0 40.0 32.0 40.0 31 37.823568703967375 40.0 38.0 40.0 32.0 40.0 32 37.819209337481766 40.0 38.0 40.0 32.0 40.0 33 37.80228163616868 40.0 38.0 40.0 32.0 40.0 34 37.82599448047953 40.0 38.0 40.0 32.0 40.0 35 37.78626799557032 40.0 38.0 40.0 32.0 40.0 36 37.715059150275096 40.0 38.0 40.0 32.0 40.0 37 37.694475206103114 40.0 38.0 40.0 32.0 40.0 38 37.730932166148115 40.0 38.0 40.0 32.0 40.0 39 37.66940884881084 40.0 38.0 40.0 32.0 40.0 40 37.65898504104484 40.0 38.0 40.0 32.0 40.0 41 37.726361862574485 40.0 38.0 40.0 32.0 40.0 42 37.68243421399568 40.0 38.0 40.0 32.0 40.0 43 37.75202587494946 40.0 38.0 40.0 32.0 40.0 44 37.67905922058746 40.0 38.0 40.0 32.0 40.0 45 37.70667440102656 40.0 38.0 40.0 32.0 40.0 46 37.553323138040746 40.0 38.0 40.0 32.0 40.0 47 37.583012533178646 40.0 38.0 40.0 32.0 40.0 48 37.59976445358505 40.0 38.0 40.0 32.0 40.0 49 37.535586844556946 40.0 38.0 40.0 32.0 40.0 50 37.527870062753784 40.0 38.0 40.0 32.0 40.0 51 37.00246093269349 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.7500976047031145 2201 2 0.809256665845254 2201 3 0.8876099175927337 2201 4 0.44088005503770944 2201 5 0.6146947708510027 2201 6 0.5888749794793711 2201 7 0.45889242771267647 2201 8 0.8711977433706934 2201 9 0.9010320307709421 2201 10 0.7154009049671117 2201 11 0.8110968131671257 2201 12 0.32158383671873736 2201 13 0.3464305512898349 2201 14 0.4953333752805662 2201 15 0.28474142375230826 2201 16 1.1263973941427707 2201 17 0.9417433919389069 2201 18 0.9254629696936547 2201 19 1.0262308488795213 2201 20 0.85804436246449 2201 21 0.38006963397168647 2201 22 0.9554176465165938 2201 23 0.8216955550511855 2201 24 0.38095778001488867 2201 25 -0.0557223016823869 2201 26 1.3032156333526288 2201 27 1.2544289971962215 2201 28 0.9923241288701874 2201 29 0.5016317909296575 2201 30 0.43837032634898065 2201 31 -0.2332247329707542 2201 32 -0.5303290579591007 2201 33 0.03297708460655713 2201 34 0.783672602391249 2201 35 0.5363625289030978 2201 36 0.5642349950682686 2201 37 0.7927117832237016 2201 38 0.8924484877024739 2201 39 0.8808859608368209 2201 40 0.8188416693775764 2201 41 0.5535933011516505 2201 42 0.8986597966893584 2201 43 0.5113773138125737 2201 44 0.713488831200884 2201 45 0.681368676644702 2201 46 0.6360284682230386 2201 47 0.12079179571166776 2201 48 0.7417883347531031 2201 49 0.6215689324831501 2201 50 0.7958076431700931 2201 51 1.461578006240714 1101 1 0.25892113411487827 1101 2 0.25852137172761047 1101 3 0.49863932935743804 1101 4 0.502644760920063 1101 5 0.4639594767333577 1101 6 0.41166909712642763 1101 7 0.7655100747714982 1101 8 0.745462449253047 1101 9 0.7510320307709364 1101 10 1.0426067873200537 1101 11 1.0500674014024227 1101 12 0.30614266024814896 1101 13 0.5589305512898335 1101 14 0.4107745517511532 1101 15 0.8943002472817199 1101 16 0.5477209235545359 1101 17 0.11086103899772581 1101 18 0.2923747343995373 1101 19 0.5600543782912908 1101 20 1.0242208330527234 1101 21 0.5080108104422791 1101 22 0.8076235288695344 1101 23 0.8364014374041275 1101 24 0.7846342506031263 1101 25 0.8354541689058479 1101 26 -0.3085490725297291 1101 27 0.7507525266079824 1101 28 0.2276182465172525 1101 29 0.9428082615178965 1101 30 0.5758703263489764 1101 31 0.45280467879395303 1101 32 0.31231800086442973 1101 33 0.8307712022536151 1101 34 0.8888196612147823 1101 35 0.9040095877266268 1101 36 0.962029112715328 1101 37 -0.15361174618806217 1101 38 0.9990661347612999 1101 39 1.0352977255426978 1101 40 0.6284004929069908 1101 41 0.7359462423281258 1101 42 0.75821862021877 1101 43 0.9385831961655171 1101 44 0.39731236061265207 1101 45 0.6659275001741136 1101 46 0.721322585870098 1101 47 0.6362329721822562 1101 48 0.8763471582825133 1101 49 -0.24828400869331801 1101 50 0.6002194078759757 1101 51 0.9711368297701242 2202 1 0.05009760470311164 2202 2 0.34675666584525544 2202 3 0.3989194414022563 2202 4 0.48849910265676044 2202 5 0.4498138184700551 2202 6 0.13351783662222516 2202 7 0.1809162372364881 2202 8 0.44560250527545264 2202 9 0.16531774505665453 2202 10 0.13980566687187235 2202 11 -0.3764031868328743 2202 12 -0.9528209251860247 2202 13 0.08988293224221167 2202 14 -1.0612142437670542 2202 15 -0.3932347667238787 2202 16 -0.2563407010953256 2202 17 -0.08087565568014554 2202 18 0.11534392207460087 2202 19 -0.04162629397762174 2202 20 0.5830443624644914 2202 21 0.3836410625431199 2202 22 0.5072033608023077 2202 23 0.6431241264797549 2202 24 0.7577434943006054 2202 25 0.6657062697461811 2202 26 0.34131087144786676 2202 27 0.734786140053366 2202 28 0.32327650982256984 2202 29 0.784965124262996 2202 30 0.7234893739680288 2202 31 0.610227647981624 2202 32 0.03990903727899564 2202 33 -0.49499910586963125 2202 34 -0.37644644522779913 2202 35 -0.19458985204928325 2202 36 0.029115947449220414 2202 37 0.47187844989036876 2202 38 0.3287580115120008 2202 39 0.25826691321776707 2202 40 0.2265797646156713 2202 41 0.15835520591355845 2202 42 0.4659217014512649 2202 43 0.5072106471459108 2202 44 0.7551554978675483 2202 45 -0.6858932281172017 2202 46 -0.8592096270150549 2202 47 0.11424417666404452 2202 48 0.524526429991198 2202 49 0.38644988486409915 2202 50 0.9469981193605648 2202 51 0.47169705385976357 1102 1 0.19034150714213638 1102 2 0.3345615438940328 1102 3 0.4116952834463916 1102 4 0.6786849330864868 1102 5 0.48146306353392987 1102 6 0.43491156484522264 1102 7 0.7372460862492645 1102 8 0.6044294506877677 1102 9 0.32908081125874133 1102 10 0.5242423683817492 1102 11 0.3492065692646875 1102 12 0.3029862757431232 1102 13 0.27600372202154233 1102 14 0.3505163021098312 1102 15 0.4252902042401132 1102 16 0.8084095892647198 1102 17 0.143572660231591 1102 18 0.10198735993755292 1102 19 0.22318206839172205 1102 20 0.20499558197668932 1102 21 0.759947682752177 1102 22 0.8843810611507408 1102 23 0.8155979940755742 1102 24 0.4702870483075756 1102 25 1.2528142836834633 1102 26 0.9446790479867744 1102 27 0.6285143630498808 1102 28 0.5581777874067768 1102 29 0.799192766539413 1102 30 0.9685532531782428 1102 31 0.7292752670292444 1102 32 0.9385733810652894 1102 33 1.1101112309480214 1102 34 1.19556284629369 1102 35 1.4058747240250469 1102 36 1.2901496292146106 1102 37 1.0125288563944395 1102 38 0.756167999897599 1102 39 0.6351542535197439 1102 40 1.0642684986458661 1102 41 1.204812813346777 1102 42 1.2257939430308227 1102 43 0.6738773138125751 1102 44 1.277513221444785 1102 45 0.7920394083520179 1102 46 0.6567601755401142 1102 47 0.8735356981506897 1102 48 1.1244102859726155 1102 49 1.1779713715075388 1102 50 1.507393009023751 1102 51 1.350907274533398 2203 1 -0.27133096672545776 2203 2 -0.34371952463093436 2203 3 0.3274908699736798 2203 4 0.6908800550377094 2203 5 -0.3716147529585214 2203 6 0.6454225985269915 2203 7 0.3832971896174371 2203 8 0.36226917194212405 2203 9 0.47484155458046473 2203 10 0.10409138115758765 2203 11 0.07597776554807467 2203 12 0.3447981224330192 2203 13 0.04226388462316777 2203 14 0.545928613375807 2203 15 0.4639080904189754 2203 16 0.874611679857054 2203 17 0.69293386812938 2203 18 1.067724874455557 2203 19 1.0059927536414293 2203 20 0.511615791035922 2203 21 0.4193553482574046 2203 22 0.6143462179451618 2203 23 0.7383622217178498 2203 24 0.8648863514434595 2203 25 1.2252300792699913 2203 26 0.7341680143050056 2203 27 1.0800242352914609 2203 28 -0.10529491874886077 2203 29 0.3682984575963246 2203 30 -0.1336534831748324 2203 31 0.6340371717911495 2203 32 0.5994328468028058 2203 33 0.39785803698750755 2203 34 0.32593450715315697 2203 35 0.18636252890309635 2203 36 0.457687376020651 2203 37 0.6623546403665586 2203 38 -0.016480083726094108 2203 39 0.8535050084558691 2203 40 1.1908654789013866 2203 41 0.43216472972308395 2203 42 0.7992550347846006 2203 43 0.6976868376221006 2203 44 0.6956316883437381 2203 45 0.6831543909304187 2203 46 1.188409420603989 2203 47 0.4594822719021394 2203 48 0.6078597633245337 2203 49 1.1007355991498144 2203 50 0.6850933574558056 2203 51 0.05503038719309217 1103 1 0.5046430592485649 1103 2 0.44902939311798207 1103 3 0.6592008266836373 1103 4 -0.15002903587138405 1103 5 -0.1887143200580894 1103 6 0.09455679766119118 1103 7 0.4054833368035844 1103 8 0.46778865246159995 1103 9 0.11012293986184574 1103 10 0.3324463595125664 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32 0.6830430350641592 2204 33 0.5326863869321414 2204 34 0.043265625647066486 2204 35 0.6398509009961231 2204 36 0.3267349950682714 2204 37 0.4796303878748702 2204 38 0.7351810458420118 2204 39 0.09658363525542057 2204 40 -0.03726298178521148 2204 41 0.7187095802214216 2204 42 0.6134853780847109 2204 43 0.8250401045102507 2204 44 0.8966283660846059 2204 45 0.509566351063306 2204 46 0.6837028868276889 2204 47 0.4018964468744528 2204 48 0.35952089289263967 2204 49 0.3759293975994282 2204 50 -0.7501225893880488 2204 51 -0.506154551898824 2205 1 0.07482287942838894 2205 2 -0.10928729019870076 2205 3 -0.09192304944023277 2205 4 -0.6278012636436117 2205 5 0.5423046609608946 2205 6 -0.25659205348766534 2205 7 -0.07366251734226381 2205 8 -0.08828027860732846 2205 9 0.14883422857313633 2205 10 -0.14407711701090875 2205 11 0.3241462637165782 2205 12 -0.5013557237208275 2205 13 -0.9751353827761022 2205 14 -0.1070017895546016 2205 15 0.069219445730333 2205 16 0.18779849304387142 2205 17 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1212 12 -0.9409161632812655 1212 13 -0.8322782399189563 1212 14 -1.4916171741699813 1212 15 -0.3373739608630757 1212 16 -1.0759377706923985 1212 17 -1.8711686959731821 1212 18 -1.2546194478887642 1212 19 -1.437230689582016 1212 20 -1.7631094836893553 1212 21 -0.8141611352590772 1212 22 -1.9680713644724221 1212 23 -1.257974774619143 1212 24 -1.0004982639411537 1212 25 -1.1145135104736 1212 26 -1.9297513996144104 1212 27 -0.6992797940125683 1212 28 -1.6318516953056346 1212 29 -1.5236429343450624 1212 30 -0.6043494538708032 1212 31 -2.803691765937792 1212 32 -2.6844499370799753 1212 33 -1.815511926382456 1212 34 -2.048607617388967 1212 35 -1.5608902183496483 1212 36 -1.1787595104262323 1212 37 -1.1343486563367335 1212 38 -0.8708756881216999 1212 39 -0.7820261270752695 1212 40 -1.4538231657872558 1212 41 -2.1530001054417554 1212 42 -2.293235807706246 1212 43 -1.3096391697039067 1212 44 -1.3757968830848313 1212 45 -0.9349774772014499 1212 46 -0.51397153177696 1212 47 -1.516708204288335 1212 48 -1.2520303465655758 1212 49 -1.3791178807036601 1212 50 -1.4742473018848514 1212 51 -0.8781197959570903 1213 1 -0.30299517880204263 1213 2 -0.050150550649590286 1213 3 -0.3310756494175777 1213 4 -0.5668519037251798 1213 5 -1.4818258477057 1213 6 -0.7493982163969193 1213 7 -0.6543807681636125 1213 8 -0.30341565869116494 1213 9 -1.729380340363079 1213 10 -1.245810435239072 1213 11 -1.3944444239462754 1213 12 -0.8172048230750804 1213 13 -0.48436841778233486 1213 14 -1.1792800267812922 1213 15 -1.1177070298559357 1213 16 -1.5915149769912489 1213 17 -0.8038751647621254 1213 18 -1.2081710509249035 1213 19 -0.15650110988336507 1213 20 -0.0406669777416937 1213 21 -1.158332427883984 1213 22 -0.6787318380194876 1213 23 -1.1138714552580922 1213 24 -1.4720061375108813 1213 25 -1.4108769408576478 1213 26 -0.6596709645855157 1213 27 -0.7431225491955331 1213 28 -1.8076758711298098 1213 29 -0.7777496523693088 1213 30 -1.1024801891149423 1213 31 -1.3789721556511694 1213 32 -1.423885758990025 1213 33 -1.0725641525068426 1213 34 -0.3546005934850385 1213 35 -0.3909570587257676 1213 36 -0.5598629430760553 1213 37 -0.5298397631680487 1213 38 -1.0146391411635065 1213 39 -0.4260728020497808 1213 40 -0.23721503165334923 1213 41 -0.7154788637967968 1213 42 -0.8847164919704369 1213 43 -0.960143304744129 1213 44 -0.4759441584898383 1213 45 -0.7914406017058155 1213 46 -0.15314678950891647 1213 47 -0.8672236682058596 1213 48 -0.4985467167932853 1213 49 -1.1947970468982945 1213 50 -0.29207895476805135 1213 51 -1.0656127154087685 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 1.0 17 2.0 18 3.0 19 3.0 20 1.0 21 8.0 22 20.0 23 39.0 24 93.0 25 145.0 26 243.0 27 337.0 28 432.0 29 639.0 30 824.0 31 1038.0 32 1229.0 33 1533.0 34 1846.0 35 2416.0 36 3329.0 37 5023.0 38 9432.0 39 28252.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.347985863239145 18.6538427724931 13.065953967612048 34.93221739665571 2 11.753124615465747 20.71475029444337 47.29903141315239 20.233093676938495 3 17.64840303046283 23.298001371091072 34.46887799047268 24.58471760797342 4 12.67204556241101 25.423192532827084 34.52161226247605 27.383149642285854 5 12.069117052505757 35.56047742094254 40.93234192901967 11.438063597532036 6 27.23373587160963 43.08390022675737 17.54469229552286 12.137671606110144 7 23.98178909806817 30.21849566700065 21.82319956406335 23.97651567086783 8 18.28472991263689 46.588971505915026 20.005624989013693 15.12067359243439 9 21.485700223241754 18.28648772170367 20.387069556504773 39.84074249854981 10 14.95192392202359 34.881963121165775 31.88665647137408 18.27945648543655 11 28.12016594009281 21.69877654338349 32.890943608493885 17.290113908029813 12 22.454253019037072 23.392923060697147 30.144667686195923 24.008156234069855 13 26.634322979837933 22.22573784035578 31.72142241909684 19.41851676070945 14 15.974968798889064 25.924168116859146 26.78373675051416 31.317126333737626 15 18.906994322276717 34.13489426778463 21.925152489936544 25.03295892000211 16 23.540579022306595 28.66459245196787 30.935681766246546 16.859146759478985 17 17.135122782963315 38.71398688674436 28.311272829545253 15.83961750074707 18 23.862258081527184 26.99291602946088 32.885092021304644 16.25973386770729 19 20.112851342087225 27.19682188120726 25.80463710031816 26.885689676387354 20 19.323595071103377 27.922797025787062 27.794476963912178 24.959130939197387 21 17.04020109335724 27.33393098841604 26.084128741936052 29.541739176290672 22 18.738244651865916 38.021410114433365 26.2405737488794 16.99977148482132 23 16.985709012287085 29.69291075603368 28.191741813004274 25.129638418674965 24 27.16518131800524 27.541352458295982 28.629436270632286 16.664029953066496 25 17.956019617149185 36.05617957777426 28.770060995974617 17.217739809101936 26 23.148587600414842 31.02005660145195 28.163616867935804 17.6677389301974 27 16.83629524161086 37.01594332823569 27.551899312696655 18.5958621174568 28 16.312468139710663 30.158730158730158 26.769674277979927 26.759127423579248 29 18.599377735590362 28.008929670059242 26.073581887535376 27.318110706815023 30 15.859232175502742 34.703276613697085 28.89888904514133 20.53860216565884 31 20.05660145195029 26.201901949410257 34.90305682996713 18.838439768672327 32 16.375749266114713 29.16556803599993 34.99622071050643 19.46246198737893 33 16.59195978132855 28.379827383149642 30.403065619012466 24.625147216509344 34 17.096450983494176 27.711859937773557 30.148183304329486 25.043505774402785 35 23.39116525163037 28.819279649844432 29.05658387385962 18.732971224665576 36 16.47418657385435 33.71477790082441 31.206384362530542 18.6046511627907 37 16.034734307159557 32.70227987835961 28.04935927859516 23.213626535885673 38 16.23512454077238 27.239009298809965 35.38118089613106 21.144685264286593 39 18.166956705162683 22.638822971048885 31.317126333737626 27.8770939900508 40 18.687268188929316 21.798590237128444 38.90031464782295 20.613826926119287 41 18.7734008332015 21.374958252034666 32.060679568985215 27.79096134577862 42 22.890189667598303 24.169874668213538 33.1839195626571 19.756016101531053 43 14.797236724147023 24.655029970644588 39.53312591186345 21.014607393344935 44 13.812863646750689 32.041343669250644 32.25579637539771 21.88999630860096 45 14.036105398231644 25.5058095589657 38.90734588409007 21.55073915871258 46 20.740389178927384 23.32261069802598 32.41048357327428 23.526516549772364 47 16.908365413348804 23.607375766844203 30.438221800348046 29.046037019458947 48 11.924976709029865 25.839793281653744 30.548963771555133 31.686266237761256 49 21.524372022710896 23.914992353530558 29.90560565311396 24.655029970644588 50 11.763258274886182 27.130025136669655 36.490358417268716 24.61635817117545 51 11.364435381802842 25.646885107579802 30.26121501898467 32.72746449163268 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 36.0 1 20.0 2 4.0 3 2.5 4 1.0 5 2.5 6 4.0 7 3.0 8 2.0 9 5.5 10 9.0 11 15.5 12 22.0 13 29.0 14 36.0 15 51.0 16 66.0 17 57.5 18 49.0 19 59.5 20 70.0 21 59.0 22 48.0 23 51.0 24 54.0 25 55.5 26 70.0 27 83.0 28 181.5 29 280.0 30 665.5 31 1051.0 32 1853.0 33 2655.0 34 4124.0 35 5593.0 36 7052.0 37 8511.0 38 9129.0 39 9747.0 40 9177.0 41 8607.0 42 7131.5 43 5656.0 44 4524.5 45 3393.0 46 2546.5 47 1700.0 48 1364.0 49 1028.0 50 1192.0 51 1356.0 52 3055.5 53 4755.0 54 2655.0 55 555.0 56 467.5 57 380.0 58 360.0 59 340.0 60 296.0 61 252.0 62 231.0 63 210.0 64 165.0 65 120.0 66 97.0 67 74.0 68 56.5 69 39.0 70 31.5 71 24.0 72 18.5 73 13.0 74 9.0 75 3.0 76 1.0 77 2.0 78 3.0 79 1.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028124945068466665 2 0.003515618133558333 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0017578090667791665 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0017578090667791665 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0017578090667791665 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 56889.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.85567522473815 #Duplication Level Percentage of deduplicated Percentage of total 1 93.46412477666784 74.63640793329989 2 4.7543979922593325 7.593313239180167 3 0.8219446570573348 1.9691083676003793 4 0.32089040273491515 1.0249967913413922 5 0.11994546405008392 0.4789163010932003 6 0.10772438323887679 0.5161442017025382 7 0.08065164659984149 0.45083441880521086 8 0.04657181560262887 0.29752190251119387 9 0.035983692740120005 0.2586151872757612 >10 0.21678815751512887 3.2258123315163165 >50 0.015488722554176083 0.8233928283242863 >100 0.013275676269373796 1.6838526700597516 >500 0.0 0.0 >1k 0.002212612710273267 7.041083827289929 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC 3985 7.004869131114979 RNA PCR Primer, Index 46 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT 221 0.3884758037581958 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA 197 0.3462883861554958 No Hit TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT 172 0.30234315948601664 RNA PCR Primer, Index 18 (95% over 21bp) GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT 137 0.2408198421487458 No Hit GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC 114 0.200390233612825 No Hit AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC 112 0.19687461547926666 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG 94 0.16523405227724164 No Hit CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC 70 0.12304663467454166 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAG 70 0.12304663467454166 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC 66 0.116015398407425 No Hit GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA 58 0.10195292587319164 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAA 57 0.1001951168064125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0017578090667791665 3 0.0 0.0 0.0017578090667791665 4 0.0 0.0 0.005273427200337499 5 0.0 0.0 0.007031236267116666 6 0.0 0.0 0.007031236267116666 7 0.0 0.0 0.007031236267116666 8 0.0 0.0 0.008789045333895832 9 0.0 0.0 0.012304663467454164 10 0.0 0.0 0.017578090667791663 11 0.0 0.0 0.01933589973457083 12 0.0 0.0 0.021093708801349996 13 0.0 0.0 0.026367136001687497 14 0.0 0.0 0.026367136001687497 15 0.0 0.0 0.031640563202025 16 0.0 0.0 0.03339837226880416 17 0.0 0.0 0.04218741760269999 18 0.0 0.0 0.058007699203712494 19 0.0 0.0 0.07734359893828333 20 0.0 0.0 0.08261702613862082 21 0.0 0.0 0.14765596160945 22 0.0 0.0 0.21796832428061663 23 0.0 0.0 0.4306632213608958 24 0.0 0.0 0.7382798080472499 25 0.0 0.0 0.7839828437835082 26 0.0 0.0 1.2427710102128706 27 0.0 0.0 1.330661463551829 28 0.0 0.0 1.446676861959254 29 0.0 0.0 1.6224577686371706 30 0.0 0.0 1.9300743553235247 31 0.0 0.0 2.8476506881822496 32 0.0 0.0 4.079874843994445 33 0.0 0.0 5.329677090474433 34 0.0 0.0 6.974986376979732 35 0.0 0.0 8.49724902881049 36 0.0 0.0 10.415018720666561 37 0.0 0.0 12.77927191548454 38 0.0 0.0 15.349188771115681 39 0.0 0.0 17.720473202200775 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 445 0.0 42.471912 45 ATGCCGT 475 0.0 39.789474 44 TATGCCG 480 0.0 39.374996 43 TCTCGTA 475 0.0 39.31579 38 CTCGTAT 475 0.0 39.31579 39 GTATGCC 480 0.0 38.906246 42 TCGTATG 480 0.0 38.906246 40 AATCTCG 460 0.0 38.641308 36 ATCTCGT 485 0.0 38.505157 37 CGTATGC 485 0.0 38.505157 41 TTCCGAA 470 0.0 38.29787 31 CCGAATC 470 0.0 38.29787 33 CGAATCT 465 0.0 38.225807 34 CGAGACC 475 0.0 37.894737 23 ATTCCGA 475 0.0 37.894737 30 GACCATT 475 0.0 37.894737 26 CCACGAG 500 0.0 37.800003 20 CACGAGA 500 0.0 37.800003 21 ACCATTC 480 0.0 37.499996 27 CATTCCG 480 0.0 37.499996 29 >>END_MODULE