##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H23NVBCXX l02n01 48h_46.34100000006af4.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8041
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.04352692451187	38.0	38.0	38.0	32.0	38.0
2	36.04738216639721	38.0	38.0	38.0	32.0	38.0
3	35.98992662604154	38.0	32.0	38.0	32.0	38.0
4	36.2152717323716	38.0	38.0	38.0	32.0	38.0
5	36.13157567466733	38.0	38.0	38.0	32.0	38.0
6	38.00534759358289	40.0	38.0	40.0	38.0	40.0
7	37.75550304688472	40.0	38.0	40.0	32.0	40.0
8	37.7459271234921	40.0	38.0	40.0	32.0	40.0
9	37.91282178833478	40.0	38.0	40.0	32.0	40.0
10	37.69406790200224	40.0	38.0	40.0	32.0	40.0
11	37.75848775027982	40.0	38.0	40.0	32.0	40.0
12	37.55005596318866	40.0	38.0	40.0	32.0	40.0
13	37.74070389255068	40.0	38.0	40.0	32.0	40.0
14	37.58636985449571	40.0	38.0	40.0	32.0	40.0
15	37.6205695808979	40.0	38.0	40.0	32.0	40.0
16	37.36189528665589	40.0	38.0	40.0	32.0	40.0
17	37.315010570824526	40.0	38.0	40.0	32.0	40.0
18	37.31227459271235	40.0	38.0	40.0	32.0	40.0
19	37.4480785971894	40.0	38.0	40.0	32.0	40.0
20	37.5618704141276	40.0	38.0	40.0	32.0	40.0
21	37.689342121626666	40.0	38.0	40.0	32.0	40.0
22	37.636861086929486	40.0	38.0	40.0	32.0	40.0
23	37.687974132570574	40.0	38.0	40.0	32.0	40.0
24	37.52891431414003	40.0	38.0	40.0	32.0	40.0
25	37.49881855490611	40.0	38.0	40.0	32.0	40.0
26	37.41673921154085	40.0	38.0	40.0	32.0	40.0
27	37.42382788210421	40.0	38.0	40.0	32.0	40.0
28	37.49732620320856	40.0	38.0	40.0	32.0	40.0
29	37.35430916552668	40.0	38.0	40.0	32.0	40.0
30	37.36686979231439	40.0	38.0	40.0	32.0	40.0
31	37.387638353438625	40.0	38.0	40.0	32.0	40.0
32	37.325830120631764	40.0	38.0	40.0	32.0	40.0
33	37.31339385648551	40.0	38.0	40.0	32.0	40.0
34	37.38826016664594	40.0	38.0	40.0	32.0	40.0
35	37.293620196492974	40.0	38.0	40.0	32.0	40.0
36	37.21738589727646	40.0	38.0	40.0	32.0	40.0
37	37.19649297351076	40.0	38.0	40.0	32.0	40.0
38	37.372714836463125	40.0	38.0	40.0	32.0	40.0
39	37.17497823653775	38.0	38.0	40.0	32.0	40.0
40	37.21751025991792	38.0	38.0	40.0	32.0	40.0
41	37.290262405173486	40.0	38.0	40.0	32.0	40.0
42	37.30655391120507	40.0	38.0	40.0	32.0	40.0
43	37.448824773038176	40.0	38.0	40.0	32.0	40.0
44	37.26762840442731	40.0	38.0	40.0	32.0	40.0
45	37.33130207685611	40.0	38.0	40.0	32.0	40.0
46	37.022509638104715	38.0	38.0	40.0	32.0	40.0
47	37.04054222111677	38.0	38.0	40.0	32.0	40.0
48	37.06591219997513	38.0	38.0	40.0	32.0	40.0
49	36.86208183061808	38.0	38.0	40.0	32.0	40.0
50	36.79890560875513	38.0	38.0	40.0	32.0	40.0
51	36.41462504663599	38.0	38.0	40.0	32.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
2201	1	1.354526723276713
2201	2	1.103429513195131
2201	3	1.2506216439810132
2201	4	1.3649366258741296
2201	5	0.7356800751331889
2201	6	1.349357283341682
2201	7	1.1732017982018021
2201	8	1.4846758710040007
2201	9	1.6822043841575223
2201	10	1.94721671557609
2201	11	1.3800418331668425
2201	12	1.6224016218156905
2201	13	1.813242031406098
2201	14	1.274724104020983
2201	15	2.1384872939560466
2201	16	2.4380290282634007
2201	17	2.1507166661463444
2201	18	1.5257964041167043
2201	19	1.7765087776806538
2201	20	2.140557164189964
2201	21	-0.45828169226607685
2201	22	1.6175364999583834
2201	23	2.1727166063103525
2201	24	1.5763450091575066
2201	25	1.7337470472236163
2201	26	1.0104905511155664
2201	27	-0.01942022820929168
2201	28	0.596113196699136
2201	29	0.8211117007992144
2201	30	1.2069907956626693
2201	31	-0.28268008033633407
2201	32	-0.03463105904511821
2201	33	1.2252072146603368
2201	34	1.6230685980686133
2201	35	0.3306076735764236
2201	36	1.7948427223817802
2201	37	0.16837556974275714
2201	38	2.5690956829628675
2201	39	1.304391832126207
2201	40	2.2411745155885683
2201	41	-0.3478161812146041
2201	42	1.0300247929154196
2201	43	0.9057252643190239
2201	44	1.6181124864718655
2201	45	1.414061784569597
2201	46	1.9142782998251846
2201	47	1.9611171380702785
2201	48	1.5244601102022912
2201	49	2.352232858287536
2201	50	2.409904938811202
2201	51	2.621890934586247
1101	1	0.48331460206459553
1101	2	1.6943386041042245
1101	3	-1.20392381056444
1101	4	0.4103911713286763
1101	5	-0.2567441672910533
1101	6	1.061478495462893
1101	7	0.748959373959373
1101	8	1.1967970831252117
1101	9	0.8791740811272248
1101	10	1.6441864125457926
1101	11	1.1224660755910847
1101	12	0.8648258642399327
1101	13	-0.7397882716241995
1101	14	1.1686634979603738
1101	15	1.9869721424408908
1101	16	2.1198472100815806
1101	17	2.0143530297827112
1101	18	2.2682206465409465
1101	19	1.988629989801865
1101	20	1.9890420126748083
1101	21	1.6780819440975563
1101	22	-0.23852410610222563
1101	23	1.687868121461868
1101	24	1.9854359182484131
1101	25	0.9761712896478585
1101	26	-0.5273882367632226
1101	27	1.9502767414876772
1101	28	0.8385374391233782
1101	29	1.7150510947386053
1101	30	2.252445341117216
1101	31	1.8688350711788217
1101	32	1.9502174258033662
1101	33	2.437328426781548
1101	34	0.11549284049285546
1101	35	1.2018197947885412
1101	36	1.870600298139351
1101	37	1.8047392061063903
1101	38	1.7509138647810474
1101	39	1.6831797109140894
1101	40	2.286629061043115
1101	41	1.8340020006035758
1101	42	1.7572975201881462
1101	43	2.299664658258415
1101	44	1.8756882440476232
1101	45	1.3383042088120263
1101	46	1.4900358755827554
1101	47	2.021723198676341
1101	48	1.7971873829295646
1101	49	1.7310207370754185
1101	50	1.2886928175990846
1101	51	-0.41598785329254184
2202	1	0.8090721778221663
2202	2	0.012520422286044663
2202	3	-0.38574199238261997
2202	4	0.2740275349650432
2202	5	-0.3552290157758975
2202	6	-2.0142790802946777
2202	7	-1.644980019980018
2202	8	-4.1516877653596325
2202	9	-2.590522888569744
2202	10	-2.5073287389693633
2202	11	0.1982236513486555
2202	12	-3.83214383272977
2202	13	-0.09584887768480854
2202	14	0.3656331949300764
2202	15	-0.8615127060439534
2202	16	-0.5619709717365993
2202	17	0.24162575705543787
2202	18	-3.746930868610569
2202	19	-1.587127585955706
2202	20	0.5041935278263239
2202	21	1.2689910350066498
2202	22	-2.3824635000416166
2202	23	-0.8272833936896475
2202	24	-2.96910953629704
2202	25	-0.9935256800491103
2202	26	-0.6258730852480667
2202	27	-1.7466929554820254
2202	28	-5.767523166937231
2202	29	-2.7243428446553324
2202	30	-1.9748273861555106
2202	31	-1.6463164439726938
2202	32	-0.9437219681360247
2202	33	-2.8657018762487496
2202	34	-1.1951132201132069
2202	35	-0.7603014173326699
2202	36	-1.7506118230727665
2202	37	-2.649806248439063
2202	38	-2.2490861352189526
2202	39	-2.24106271332834
2202	40	-2.1224618480477915
2202	41	-0.8932707266691509
2202	42	-1.9699752070845804
2202	43	0.6329979915917505
2202	44	-2.109160240800861
2202	45	-1.7677563972485828
2202	46	-3.812994427447542
2202	47	-1.4025192255660883
2202	48	-2.293721707979529
2202	49	-2.0114035053488237
2202	50	-1.3173677884615245
2202	51	-2.196290883595573
1102	1	0.13075049950049333
1102	2	0.8097232194888377
1102	3	-0.9731545797952066
1102	4	0.9873142482517565
1102	5	0.4419737814268956
1102	6	-0.6436497096653113
1102	7	0.005369630369628453
1102	8	0.7993611856893139
1102	9	0.6612253631785023
1102	10	-0.9968392284798497
1102	11	0.4429788961039023
1102	12	1.1468771462912102
1102	13	1.253801471965545
1102	14	-0.15184932255244377
1102	15	1.2433823988511463
1102	16	-1.3801527899184194
1102	17	-0.17795466252498215
1102	18	-1.5651126867923892
1102	19	-0.985728984557106
1102	20	0.3992984229312242
1102	21	1.4088511748667898
1102	22	-0.34749846507658333
1102	23	0.5083809419746856
1102	24	0.6392820720945664
1102	25	0.18129949477606289
1102	26	0.5559450965701132
1102	27	1.2323280235389547
1102	28	1.4282810288669623
1102	29	1.1637690434565542
1102	30	-0.09370850503663064
1102	31	-1.0798828775391272
1102	32	0.7066276822136217
1102	33	0.4629694524225769
1102	34	-3.5447635697635604
1102	35	-2.214846871878123
1102	36	-0.7576048300657732
1102	37	-2.887568486201303
1102	38	-1.6337015198343394
1102	39	-0.22707669934232655
1102	40	-1.6749094004953449
1102	41	-0.6275364609348841
1102	42	0.7572975201881462
1102	43	-0.597771239177483
1102	44	0.9397908081501924
1102	45	1.1203554908633038
1102	46	-0.3304769449300622
1102	47	-1.1577639808108415
1102	48	0.10487969062187119
1102	49	-1.1792356731809974
1102	50	-1.044640515734251
1102	51	1.0134993261946406
2203	1	0.39998126873125983
2203	2	-0.8889947292291112
2203	3	0.2960761894355599
2203	4	1.4103911713286763
2203	5	1.3265891660422824
2203	6	0.7281451621295645
2203	7	1.0822927072927087
2203	8	0.8634637497918831
2203	9	0.12917408112722484
2203	10	1.4775197458791212
2203	11	1.1224660755910847
2203	12	-0.5518408024267387
2203	13	0.6768783950424648
2203	14	0.5019968312937095
2203	15	0.6536388091075622
2203	16	0.7865138767482449
2203	17	0.5143530297827112
2203	18	0.43488731320761076
2203	19	1.1552966564685363
2203	20	0.4890420126748083
2203	21	1.3447486107642206
2203	22	1.0114758938977744
2203	23	-0.22879854520480336
2203	24	0.48543591824841315
2203	25	1.9761712896478585
2203	26	-0.8607215700965511
2203	27	-0.04972325851232284
2203	28	-0.4114625608766218
2203	29	1.3817177614052767
2203	30	-0.49755465888278394
2203	31	0.8688350711788217
2203	32	1.6168840924700376
2203	33	0.02066176011488352
2203	34	-0.8845071595071445
2203	35	0.20181979478854117
2203	36	-0.379399701860649
2203	37	-1.2785941272269454
2203	38	-0.6657528018856169
2203	39	1.0998463775807537
2203	40	-0.6300376056235493
2203	41	-0.5826646660630885
2203	42	-2.2427024798118538
2203	43	-0.7836686750749138
2203	44	1.3756882440476232
2203	45	-1.5783624578546451
2203	46	-2.0099641244172446
2203	47	1.0217231986763409
2203	48	-2.2028126170704354
2203	49	-0.2689792629245815
2203	50	-2.7113071824009154
2203	51	-0.9993211866258704
1103	1	-3.6384802697302803
1103	2	-3.036430626665009
1103	3	-2.2808468874875203
1103	4	0.06423732517482961
1103	5	-0.4811031416500242
1103	6	0.3563502903346887
1103	7	0.6976773226773219
1103	8	1.4147458010739342
1103	9	1.2766099785631226
1103	10	1.387776156135537
1103	11	0.4429788961039023
1103	12	-1.3915843921703228
1103	13	-0.36158314341907527
1103	14	0.0019968312937095334
1103	15	-0.6796945242257735
1103	16	-1.3801527899184194
1103	17	-0.639493124063442
1103	18	-1.488189609869309
1103	19	1.091194092365967
1103	20	-1.2930092693764692
1103	21	-2.1296103635947503
1103	22	-0.6551907727688899
1103	23	-0.26084982725608086
1103	24	-1.3607179279054336
1103	25	0.7966841101606761
1103	26	-1.520977980352967
1103	27	-0.5369027456918118
1103	28	-0.8024882019022641
1103	29	1.1637690434565542
1103	30	-3.4783238896520174
1103	31	-0.46449826215451395
1103	32	-5.062603087017145
1103	33	-1.6139536245005033
1103	34	1.6090825840826
1103	35	-2.59946225649351
1103	36	0.9347028622419202
1103	37	1.72781612918331
1103	38	-1.0183169044497191
1103	39	-2.3039997762653996
1103	40	-1.0595247851107317
1103	41	-0.08907492247334403
1103	42	-1.2427024798118538
1103	43	-0.6746943161005561
1103	44	0.5551754235348056
1103	45	-1.4181060475982292
1103	46	-1.0997077141608287
1103	47	-1.6962255192723816
1103	48	-2.2797356939935156
1103	49	0.4361489422036229
1103	50	-2.583102054195791
1103	51	-0.29419298149766604
2204	1	0.515365884115873
2204	2	1.2712616810273047
2204	3	-0.5116161182567467
2204	4	0.1411604020979098
2204	5	0.4419737814268956
2204	6	-1.4128804788960778
2204	7	0.6976773226773219
2204	8	-0.9698695835414526
2204	9	-0.8003130983599576
2204	10	1.2339300022893767
2204	11	-0.5570211038960977
2204	12	-0.3146613152472497
2204	13	1.4076476258116983
2204	14	1.3866122159090963
2204	15	0.9356900911588397
2204	16	-0.2263066360722661
2204	17	-0.9471854317557487
2204	18	0.28104115936145746
2204	19	0.1681171692890473
2204	20	1.0916061152389105
2204	21	0.9473127133283228
2204	22	-0.34749846507658333
2204	23	1.2776117112054521
2204	24	-1.6684102355977402
2204	25	-1.203315889839324
2204	26	-1.2902087495837335
2204	27	1.386174177385108
2204	28	-0.03325743267149761
2204	29	1.7791536588411745
2204	30	-1.0167854281135504
2204	31	1.6124248147685663
2204	32	1.0912430668290085
2204	33	1.1552771447302703
2204	34	-0.6216866466866335
2204	35	2.0159223588911104
2204	36	2.0885490160880735
2204	37	0.9585853599525436
2204	38	1.2124523263195073
2204	39	1.003692531426907
2204	40	0.786629061043115
2204	41	-0.012151845550270934
2204	42	0.9111436740342995
2204	43	0.09453645313021042
2204	44	-2.0602091918498076
2204	45	1.4280477985556104
2204	46	1.9002922858391713
2204	47	0.6883898653430052
2204	48	0.6433412290834113
2204	49	0.5899950960497762
2204	50	-1.1984866695804044
2204	51	0.09042240311771366
2205	1	0.6142669830169751
2205	2	0.48005288981850924
2205	3	-0.2753523819930095
2205	4	-1.5181802572427543
2205	5	0.255160594613713
2205	6	1.3233832573676594
2205	7	1.5108641358641393
2205	8	1.1729875593156933
2205	9	0.40298360493674323
2205	10	0.7394245077838875
2205	11	0.3605613136863184
2205	12	1.1029211023351664
2205	13	1.4625926807567495
2205	14	1.2877111170079942
2205	15	1.3203054757742265
2205	16	-2.2372956470612735
2205	17	-1.7713612559315735
2205	18	0.5063158846361802
2205	19	-1.1780366768647994
2205	20	0.2509467745795746
2205	21	0.6066533726689869
2205	22	0.6305235129453948
2205	23	1.3545347881285323
2205	24	1.7711502039626978
2205	25	0.4285522420288075
2205	26	0.48451652514154375
2205	27	0.7598005510114874
2205	28	0.2909183915043272
2205	29	-0.5230441433566284
2205	30	-0.8189832303113533
2205	31	1.1069303092740554
2205	32	-0.002163526577582786
2205	33	1.342090331543453
2205	34	1.3893023643023739
2205	35	-0.07198972902097722
2205	36	0.20393363147268673
2205	37	-1.0524036510364638
2205	38	0.4651995790667627
2205	39	0.8498463775807537
2205	40	0.9294862039002609
2205	41	0.9768591434607217
2205	42	0.6144403773310003
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1211	7	-1.6869380619380578
1211	8	-2.8160234296952993
1211	9	-0.6464669445138043
1211	10	-1.150685382326003
1211	11	-1.3262518731268642
1211	12	-1.930045930631863
1211	13	-4.592352374188302
1211	14	-1.6133877840909037
1211	15	-1.6027714473026933
1211	16	-1.3801527899184194
1211	17	-2.024108508678829
1211	18	-3.7958819175616227
1211	19	-2.2934212922494126
1211	20	-1.216086192453396
1211	21	-2.437302671287057
1211	22	-2.4244215419996564
1211	23	-0.799311365717621
1211	24	-2.591487158674667
1211	25	-2.0494697359931706
1211	26	-0.6748241341991204
1211	27	-0.613825822614892
1211	28	-0.4947958942099575
1211	29	-2.374692495004979
1211	30	-0.09370850503663064
1211	31	-2.002959800616054
1211	32	-2.754910779324838
1211	33	-4.998569009115883
1211	34	-2.1601481851481736
1211	35	-0.8302314872627363
1211	36	-1.6806817531427
1211	37	-3.6567992554320696
1211	38	-1.941393827526646
1211	39	-5.380922853188476
1211	40	-2.213370938956885
1211	41	-6.089074922473348
1211	42	-1.6273178644272406
1211	43	-1.7516173930236363
1211	44	-0.5986707303113477
1211	45	0.043432413940230674
1211	46	-2.715092329545449
1211	47	-2.6193024423493014
1211	48	-3.587428001685822
1211	49	-3.948466442411764
1211	50	-1.967717438811171
1211	51	-0.8326545199592061
1208	1	1.8999812687312598
1208	2	0.34818475795037784
1208	3	0.8729992663586401
1208	4	0.06423732517482961
1208	5	0.4419737814268956
1208	6	1.1255810595654623
1208	7	0.5438311688311686
1208	8	0.18397657030470072
1208	9	1.2766099785631226
1208	10	1.2339300022893767
1208	11	1.6737481268731358
1208	12	0.531492530906597
1208	13	1.5614937796578516
1208	14	1.5404583697552496
1208	15	1.2433823988511463
1208	16	1.6967702870046608
1208	17	1.437429952859631
1208	18	1.511810390130691
1208	19	1.7065787077505874
1208	20	1.2454522690850638
1208	21	0.9473127133283228
1208	22	1.4217323041541903
1208	23	-0.6454652118714677
1208	24	1.1008205336330263
1208	25	1.4120687255452893
1208	26	1.6328681734931934
1208	27	1.2323280235389547
1208	28	1.735973336559276
1208	29	1.7791536588411745
1208	30	0.06013764880952266
1208	31	0.9201171224608728
1208	32	1.7066276822136217
1208	33	-2.6139536245005033
1208	34	1.147544122544133
1208	35	1.862076205044957
1208	36	1.6270105545496065
1208	37	2.0355084368756238
1208	38	-0.24908613521895262
1208	39	1.7729233006576735
1208	40	1.5558598302738815
1208	41	1.910925077526656
1208	42	0.7572975201881462
1208	43	-0.21315585456209618
1208	44	-0.752516884157501
1208	45	-0.18733681682900283
1208	46	1.0541384396853246
1208	47	-1.2346870577339146
1208	48	1.027956613698798
1208	49	-0.2561587501040705
1208	50	-0.8138712849650176
1208	51	-0.29419298149766604
1209	1	0.8999812687312598
1209	2	2.1943386041042245
1209	3	0.7960761894355599
1209	4	1.4103911713286763
1209	5	-0.17341083395771761
1209	6	1.561478495462893
1209	7	1.0822927072927087
1209	8	1.1967970831252117
1209	9	1.3791740811272248
1209	10	1.4775197458791212
1209	11	1.6224660755910847
1209	12	1.1981591975732613
1209	13	1.0102117283758005
1209	14	1.0019968312937095
1209	15	1.1536388091075622
1209	16	1.6198472100815806
1209	17	-0.23564697021728875
1209	18	0.9348873132076108
1209	19	0.8219633231352006
1209	20	1.6557086793414797
1209	21	1.011415277430892
1209	22	1.34480922723111
1209	23	2.0212014547951966
1209	24	1.3187692515817488
1209	25	0.8928379563145228
1209	26	0.8892784299034489
1209	27	1.2836100748210058
1209	28	0.5052041057900425
1209	29	-0.034948905261394714
1209	30	1.9191120077838804
1209	31	1.535501737845486
1209	32	1.783550759136702
1209	33	1.6039950934482192
1209	34	0.9488261738261841
1209	35	-0.13151353854478742
1209	36	-3.2127330351939776
1209	37	0.13807253943972597
1209	38	1.584247198114383
1209	39	1.1831797109140894
1209	40	1.2032957277097793
1209	41	1.0840020006035758
1209	42	0.5072975201881462
1209	43	1.7996646582584148
1209	44	-0.20764508928570535
1209	45	-0.07836245785464513
1209	46	-0.9266307910839089
1209	47	0.6050565320096695
1209	48	1.1305207162629003
1209	49	1.314354070408747
1209	50	-0.1279738490675868
1209	51	1.1673454800407939
1214	1	0.05382742257741313
1214	2	1.2712616810273047
1214	3	1.3345377278971
1214	4	0.9873142482517565
1214	5	0.4419737814268956
1214	6	1.5871195211039222
1214	7	0.8515234765234752
1214	8	-1.4314080450799125
1214	9	1.1227638247169693
1214	10	1.6954684638278437
1214	11	1.5199019730269825
1214	12	1.1468771462912102
1214	13	0.9461091642732313
1214	14	-3.228772399475524
1214	15	-1.1412329857642334
1214	16	-1.226306636072266
1214	17	-0.17795466252498215
1214	18	1.2041180824383773
1214	19	0.7835017846736605
1214	20	-0.677624653991856
1214	21	0.9473127133283228
1214	22	1.2678861503080299
1214	23	-1.337772904179161
1214	24	1.5623589951714933
1214	25	1.1043764178529827
1214	26	1.017483558108573
1214	27	-1.4599796687687387
1214	28	-0.3409497403638042
1214	29	0.7022305819180943
1214	30	1.1370607257326029
1214	31	-0.31065210830836065
1214	32	-0.8318338562479113
1214	33	0.15527714473027032
1214	34	0.9936979686979797
1214	35	-0.9840776411088896
1214	36	0.85777978531884
1214	37	1.0355084368756238
1214	38	1.3662984801656606
1214	39	0.542154069888447
1214	40	0.9404752148892683
1214	41	-1.6275364609348841
1214	42	1.3726821355727594
1214	43	0.7099210685148307
1214	44	-0.44482457646519435
1214	45	0.7357401062479241
1214	46	1.4387538243007043
1214	47	1.9191590961122387
1214	48	-0.3566587709165887
1214	49	1.2823027883574696
1214	50	1.4168979458042088
1214	51	-0.7557314430361259
1215	1	0.05382742257741313
1215	2	0.8097232194888377
1215	3	0.4114608048201731
1215	4	-0.3973011363636303
1215	5	0.4419737814268956
1215	6	0.971734905719309
1215	7	1.0053696303696285
1215	8	0.6455150318431606
1215	9	-1.1080054060522642
1215	10	-0.6122238438644629
1215	11	-0.8647134115884043
1215	12	-1.2377382383241695
1215	13	-1.1308139126498418
1215	14	-1.1518493225524438
1215	15	-1.5258483703796202
1215	16	-2.072460482226113
1215	17	-4.485646970217289
1215	18	-1.1035742252539293
1215	19	-2.2934212922494126
1215	20	-1.3699323462995494
1215	21	-1.0526872866716772
1215	22	-4.19365231123043
1215	23	-1.6454652118714677
1215	24	-0.8222563894438935
1215	25	-1.5879312744547107
1215	26	-3.59790105727604
1215	27	-1.9984412072302717
1215	28	0.1975117980977359
1215	29	0.3945382742257877
1215	30	1.3678299565018293
1215	31	-2.1568059544622074
1215	32	-0.3702953947094514
1215	33	-1.46010747065435
1215	34	-0.9293789543789401
1215	35	-1.8302314872627363
1215	36	0.4731644007034532
1215	37	-1.27218387081669
1215	38	-1.1721630582958724
1215	39	-2.457845930111553
1215	40	-0.05952478511073167
1215	41	-1.9352287686271907
1215	42	-0.5503947875041604
1215	43	-0.21315585456209618
1215	44	-0.9832861149267345
1215	45	-1.5719522014443896
1215	46	-1.4074000218531424
1215	47	-5.080840903887761
1215	48	-2.895120309378129
1215	49	-2.3330818270271507
1215	50	-0.8138712849650176
1215	51	-0.9865006738053594
1212	1	0.39998126873125983
1212	2	1.0693386041042245
1212	3	1.42107618943556
1212	4	1.1603911713286763
1212	5	-0.048410833957717614
1212	6	0.7698118287962288
1212	7	1.5822927072927087
1212	8	0.6551304164585474
1212	9	0.42084074779388914
1212	10	-0.06414692078754314
1212	11	0.789132742257749
1212	12	-2.156007469093403
1212	13	-0.7606216049575352
1212	14	0.8769968312937095
1212	15	0.07030547577422652
1212	16	-1.0051527899184194
1212	17	-0.29814697021728875
1212	18	-0.25261268679238924
1212	19	-0.8030366768647994
1212	20	0.884875346008144
1212	21	1.5530819440975563
1212	22	1.21980922723111
1212	23	-1.8954652118714677
1212	24	-1.5145640817515869
1212	25	-0.2946620436854772
1212	26	1.0559450965701132
1212	27	0.054443408154341455
1212	28	-0.3072958942099575
1212	29	-0.451615571928059
1212	30	0.37744534111721606
1212	31	0.535501737845486
1212	32	0.4710507591367019
1212	33	-1.6043382398851165
1212	34	0.28215950715951976
1212	35	1.2851531281218769
1212	36	0.7664336314726867
1212	37	-0.5077607938936097
1212	38	0.18841386478104738
1212	39	-1.2751536224192463
1212	40	0.09912906104311503
1212	41	-0.35349799939642423
1212	42	-0.6177024798118538
1212	43	0.13299799159175052
1212	44	1.334021577380959
1212	45	0.5674708754786906
1212	46	1.198369208916091
1212	47	2.063389865343005
1212	48	-0.015312617070435408
1212	49	-0.9148125962579172
1212	50	-1.544640515734251
1212	51	-0.5201545199592061
1213	1	-1.0166853979354045
1213	2	0.19433860410422454
1213	3	-2.287257143897776
1213	4	-2.1729421620046594
1213	5	0.4099224993756181
1213	6	0.06147849546289308
1213	7	-2.001040626040627
1213	8	1.1967970831252117
1213	9	1.2125074144605534
1213	10	0.8108530792124569
1213	11	0.6224660755910847
1213	12	-1.8851741357600673
1213	13	0.6768783950424648
1213	14	0.16866349796037383
1213	15	0.15363880910756222
1213	16	0.5365138767482449
1213	17	-0.8189803035506245
1213	18	-1.3151126867923892
1213	19	-1.9280366768647994
1213	20	-2.3442913206585203
1213	21	-0.738584722569108
1213	22	-0.3218574394355542
1213	23	-0.06213187853813196
1213	24	-1.7645640817515869
1213	25	-1.690495377018813
1213	26	0.22261176323677745
1213	27	1.4502767414876772
1213	28	-0.4114625608766218
1213	29	-0.201615571928059
1213	30	-1.497554658882784
1213	31	-0.46449826215451395
1213	32	0.2002174258033662
1213	33	-0.3126715732184522
1213	34	-0.38450715950714454
1213	35	-1.3815135385447874
1213	36	-1.7960663685273133
1213	37	-0.44526079389360973
1213	38	-4.165752801885617
1213	39	-0.40015362241924635
1213	40	-1.2967042722902207
1213	41	-2.49933133272976
1213	42	0.6739641868548105
1213	43	1.1329979915917505
1213	44	0.709021577380959
1213	45	-1.2450291245213094
1213	46	-0.7599641244172446
1213	47	0.10505653200966947
1213	48	1.6305207162629003
1213	49	1.8976874037420828
1213	50	0.6220261509324132
1213	51	-0.5826545199592061
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	3.0
22	5.0
23	14.0
24	21.0
25	30.0
26	50.0
27	78.0
28	100.0
29	131.0
30	134.0
31	182.0
32	216.0
33	257.0
34	293.0
35	400.0
36	477.0
37	786.0
38	1346.0
39	3518.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.23787011694451	18.997263000746454	14.854441403334162	39.91042547897487
2	9.638104713344111	19.960203954732	55.26675786593708	15.134933465986816
3	13.642581768436763	23.98955353811715	46.07635866185798	16.29150603158811
4	9.165526675786593	38.16689466484268	32.71981096878498	19.94776769058575
5	11.030966297724163	30.49371968660614	49.29735107573685	9.177962939932845
6	20.992413878870785	53.00335779131948	16.0676532769556	9.936575052854122
7	16.78895659743813	29.02624051734859	20.743688595945777	33.4411142892675
8	15.18467852257182	53.115284168635746	18.64195995522945	13.058077353562988
9	17.659495087675662	17.60975003109066	19.43788086058948	45.2928740206442
10	11.752269618206691	45.45454545454545	28.168138291257307	14.625046635990548
11	22.6464370103221	21.25357542594205	42.86780251212536	13.232185051610495
12	15.134933465986816	23.268250217634623	27.95672180077105	33.64009451560751
13	18.43054346474319	20.955105086432035	44.07412013431165	16.54023131451312
14	12.933714712100485	22.12411391617958	24.26315134933466	40.679020022385274
15	16.378559880611864	28.19301081954981	20.29598308668076	35.13244621315757
16	34.53550553413754	26.588732744683497	23.877627160800895	14.998134560378062
17	15.682129088421837	46.73548066160925	23.081706255440864	14.500683994528044
18	34.261907722920036	26.240517348588483	25.34510633005845	14.15246859843303
19	16.378559880611864	24.30046014177341	24.238278821042158	35.08270115657257
20	15.781619201591843	25.85499316005472	22.845417236662108	35.517970401691336
21	14.475811466235545	26.02910085810223	22.721054595199604	36.77403308046263
22	14.550429051113046	45.52916303942296	22.5718194254446	17.348588484019402
23	14.7121004850143	25.606267877129714	23.93980848153215	35.74182315632384
24	35.18219126974257	24.909837084939685	24.362641462504662	15.545330182813084
25	15.358786220619326	45.00683994528043	23.98955353811715	15.644820295983086
26	34.93346598681756	25.3077975376197	25.29536127347345	14.463375202089294
27	15.17224225842557	45.52916303942296	24.312896405919663	14.985698296231812
28	14.040542221116775	26.414625046635994	23.865190896654646	35.67964183559259
29	16.75164780499938	24.934709613232183	23.355304066658373	34.95833851511006
30	14.017412935323383	44.71393034825871	25.062189054726367	16.206467661691544
31	15.396095013058078	25.046635990548438	43.20358164407412	16.353687352319362
32	14.848899390623057	26.414625046635994	42.85536624797911	15.881109314761845
33	15.48314886208183	23.49210297226713	25.693321726153464	35.33142643949758
34	16.104962069394354	23.317995274219623	25.631140405422208	34.94590225096381
35	33.4411142892675	25.046635990548438	24.661111802014673	16.851137918169382
36	14.537992786966795	43.87513990797164	25.34510633005845	16.241760975003107
37	14.189777390871782	43.57666956846163	25.556522820544707	16.677030220121875
38	15.20955105086432	21.974878746424576	45.491854246984204	17.323715955726897
39	14.376321353065538	21.676408406914565	27.098619574679766	36.84865066534013
40	15.806491729884343	21.266011690088295	45.87737843551797	17.050118144509387
41	15.644820295983086	20.731252331799528	26.38975251834349	37.234174853873895
42	34.98321104340256	20.171620445218256	27.882104215893545	16.963064295485637
43	13.493346598681754	22.37283919910459	47.64332794428554	16.49048625792812
44	13.58040044770551	41.561994776769055	27.397089914189777	17.460514861335653
45	13.891307051361771	21.726153463499564	46.98420594453426	17.3983335406044
46	33.4411142892675	21.203830369357046	26.538987688098498	18.816067653276956
47	12.896405919661733	22.89516229324711	26.041537122248474	38.16689466484268
48	13.990797164531774	20.470090784728267	26.327571197612237	39.211540853127715
49	32.607884591468725	21.639099614475814	26.974256933217262	18.778758860838206
50	11.76470588235294	21.514736973013306	46.18828503917423	20.53227210545952
51	11.304564108941674	21.203830369357046	27.334908593458522	40.15669692824276
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.5
4	2.0
5	1.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	2.5
12	5.0
13	4.5
14	4.0
15	7.5
16	11.0
17	6.0
18	1.0
19	3.0
20	5.0
21	3.5
22	2.0
23	4.0
24	6.0
25	6.5
26	18.0
27	29.0
28	66.5
29	104.0
30	237.5
31	371.0
32	613.0
33	855.0
34	1034.0
35	1213.0
36	1197.5
37	1182.0
38	985.5
39	789.0
40	607.5
41	426.0
42	324.5
43	223.0
44	189.0
45	155.0
46	160.5
47	166.0
48	166.5
49	167.0
50	249.5
51	332.0
52	1017.5
53	1703.0
54	904.0
55	105.0
56	86.0
57	67.0
58	49.0
59	31.0
60	28.5
61	26.0
62	22.0
63	18.0
64	16.0
65	14.0
66	11.0
67	8.0
68	6.5
69	5.0
70	3.5
71	2.0
72	2.0
73	2.0
74	2.5
75	2.0
76	1.0
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0373087924387514
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.012436264146250466
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	8041.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.17622186295237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.95669291338582	53.04066658375823
2	10.196850393700787	12.883969655515484
3	3.7007874015748032	7.014052978485264
4	1.0433070866141732	2.636487999005099
5	0.37401574803149606	1.1814450938937944
6	0.3346456692913386	1.2684989429175475
7	0.1377952755905512	0.6093769431662729
8	0.07874015748031496	0.3979604526800149
9	0.03937007874015748	0.2238527546325084
>10	0.09842519685039369	1.5047879616963065
>50	0.01968503937007874	0.8581022260912822
>100	0.0	0.0
>500	0.0	0.0
>1k	0.01968503937007874	18.38079840815819
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC	1478	18.38079840815819	RNA PCR Primer, Index 46 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT	69	0.8581022260912822	RNA PCR Primer, Index 18 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	31	0.38552418853376447	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT	31	0.38552418853376447	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC	23	0.28603407536376074	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG	22	0.27359781121751026	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	14	0.17410769804750653	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA	9	0.1119263773162542	No Hit
TCTAGTGGGTATCTACATGCAATATGGAATCTGGACACTTTGTGAATACCC	9	0.1119263773162542	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.012436264146250466
10	0.0	0.0	0.012436264146250466
11	0.0	0.0	0.012436264146250466
12	0.0	0.0	0.012436264146250466
13	0.0	0.0	0.012436264146250466
14	0.0	0.0	0.012436264146250466
15	0.0	0.0	0.012436264146250466
16	0.0	0.0	0.012436264146250466
17	0.0	0.0	0.012436264146250466
18	0.0	0.0	0.09949011317000372
19	0.0	0.0	0.1119263773162542
20	0.0	0.0	0.1119263773162542
21	0.0	0.0	0.1119263773162542
22	0.0	0.0	0.12436264146250467
23	0.0	0.0	0.12436264146250467
24	0.0	0.0	0.12436264146250467
25	0.0	0.0	0.12436264146250467
26	0.0	0.0	0.1492351697550056
27	0.0	0.0	0.21141649048625794
28	0.0	0.0	0.2238527546325084
29	0.0	0.0	0.27359781121751026
30	0.0	0.0	0.4103967168262654
31	0.0	0.0	0.7213033204825271
32	0.0	0.0	1.2933714712100486
33	0.0	0.0	1.9027484143763214
34	0.0	0.0	2.823031961198856
35	0.0	0.0	3.681134187290138
36	0.0	0.0	4.875015545330183
37	0.0	0.0	6.031588110931477
38	0.0	0.0	7.387140902872777
39	0.0	0.0	8.755129958960328
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATGCC	180	0.0	45.000004	42
TATGCCG	180	0.0	45.000004	43
CTCGTAT	180	0.0	45.000004	39
AATCTCG	185	0.0	45.0	36
ATCTCGT	185	0.0	45.0	37
TGCCGTC	175	0.0	45.0	45
TTCCGAA	185	0.0	45.0	31
ATGCCGT	175	0.0	45.0	44
CGAATCT	185	0.0	45.0	34
GAATCTC	185	0.0	45.0	35
TCCGAAT	185	0.0	45.0	32
CCGAATC	185	0.0	45.0	33
ACCATTC	190	0.0	43.815792	27
TCTCGTA	190	0.0	43.815792	38
CCATTCC	190	0.0	43.815792	28
CCGAGCC	190	0.0	43.815792	14
TCTCCGA	190	0.0	43.815792	11
ATTCCGA	190	0.0	43.815792	30
TCCGAGC	190	0.0	43.815792	13
CTCCGAG	190	0.0	43.815792	12
>>END_MODULE