FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_46.34100000006af4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_46.34100000006af4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8041
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC147818.38079840815819RNA PCR Primer, Index 46 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT690.8581022260912822RNA PCR Primer, Index 18 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC310.38552418853376447No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT310.38552418853376447No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC230.28603407536376074No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG220.27359781121751026No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC140.17410769804750653No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA90.1119263773162542No Hit
TCTAGTGGGTATCTACATGCAATATGGAATCTGGACACTTTGTGAATACCC90.1119263773162542No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC1800.045.00000442
TATGCCG1800.045.00000443
CTCGTAT1800.045.00000439
AATCTCG1850.045.036
ATCTCGT1850.045.037
TGCCGTC1750.045.045
TTCCGAA1850.045.031
ATGCCGT1750.045.044
CGAATCT1850.045.034
GAATCTC1850.045.035
TCCGAAT1850.045.032
CCGAATC1850.045.033
ACCATTC1900.043.81579227
TCTCGTA1900.043.81579238
CCATTCC1900.043.81579228
CCGAGCC1900.043.81579214
TCTCCGA1900.043.81579211
ATTCCGA1900.043.81579230
TCCGAGC1900.043.81579213
CTCCGAG1900.043.81579212