Basic Statistics
Measure | Value |
---|---|
Filename | H23NVBCXX l02n01 48h_46.34100000006af4.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8041 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC | 1478 | 18.38079840815819 | RNA PCR Primer, Index 46 (95% over 21bp) |
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT | 69 | 0.8581022260912822 | RNA PCR Primer, Index 18 (95% over 21bp) |
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC | 31 | 0.38552418853376447 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT | 31 | 0.38552418853376447 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC | 23 | 0.28603407536376074 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG | 22 | 0.27359781121751026 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC | 14 | 0.17410769804750653 | No Hit |
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA | 9 | 0.1119263773162542 | No Hit |
TCTAGTGGGTATCTACATGCAATATGGAATCTGGACACTTTGTGAATACCC | 9 | 0.1119263773162542 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTATGCC | 180 | 0.0 | 45.000004 | 42 |
TATGCCG | 180 | 0.0 | 45.000004 | 43 |
CTCGTAT | 180 | 0.0 | 45.000004 | 39 |
AATCTCG | 185 | 0.0 | 45.0 | 36 |
ATCTCGT | 185 | 0.0 | 45.0 | 37 |
TGCCGTC | 175 | 0.0 | 45.0 | 45 |
TTCCGAA | 185 | 0.0 | 45.0 | 31 |
ATGCCGT | 175 | 0.0 | 45.0 | 44 |
CGAATCT | 185 | 0.0 | 45.0 | 34 |
GAATCTC | 185 | 0.0 | 45.0 | 35 |
TCCGAAT | 185 | 0.0 | 45.0 | 32 |
CCGAATC | 185 | 0.0 | 45.0 | 33 |
ACCATTC | 190 | 0.0 | 43.815792 | 27 |
TCTCGTA | 190 | 0.0 | 43.815792 | 38 |
CCATTCC | 190 | 0.0 | 43.815792 | 28 |
CCGAGCC | 190 | 0.0 | 43.815792 | 14 |
TCTCCGA | 190 | 0.0 | 43.815792 | 11 |
ATTCCGA | 190 | 0.0 | 43.815792 | 30 |
TCCGAGC | 190 | 0.0 | 43.815792 | 13 |
CTCCGAG | 190 | 0.0 | 43.815792 | 12 |