FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_45.34100000006ae7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_45.34100000006ae7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108524
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC12841.1831484279974938RNA PCR Primer, Index 46 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAG1900.041.463512
TCGTATG1900.041.44439740
CCGAGCC1950.040.40033714
CGAGCCC1950.040.40033715
TCTCCGA1950.040.40033711
CGTATGC1950.040.3817241
TTCCGAA1750.039.85428631
GACCATT1750.039.85428626
CCGAATC1750.039.85428633
ATCTCGT2000.039.37217737
GCCCACG2000.039.37217718
CCCACGA2000.039.37217719
TCTCGTA2000.039.37217738
ATGCCGT2000.039.37217744
CCACGAG2000.039.37217720
AATCTCG1900.039.07614536
CATTCCG1850.038.9161329
TCCGAAT1800.038.74722332
ATTCCGA1800.038.74722330
CGAGACC1800.038.74722323
Produced by FastQC (version 0.11.2)