H23NVBCXX l02n01 48h_44.34100000006ada.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 48h_44.34100000006ada.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	85552
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC	1435	1.6773424350102861	RNA PCR Primer, Index 46 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	247	0.28871329717598654	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA	191	0.22325603141948755	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT	186	0.21741163269122873	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA	180	0.21039835421731812	No Hit
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT	146	0.17065644286515805	RNA PCR Primer, Index 18 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	123	0.14377220871516738	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC	119	0.1390966897325603	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAG	90	0.10519917710865906	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCTAA	30	1.1311134E-4	37.500004	1
GTATAGG	25	0.0020962488	36.0	1
GCCGTCT	45	8.5754436E-7	35.000004	45
ATGCCGT	170	0.0	34.411762	44
CATTCCG	165	0.0	34.09091	29
ATTCCGA	165	0.0	34.09091	30
CGAGACC	165	0.0	34.09091	23
CCGAATC	165	0.0	34.09091	33
GCCCACG	175	0.0	33.42857	18
TATGCCG	175	0.0	33.42857	43
CCGAGCC	175	0.0	33.42857	14
TCGTATG	175	0.0	33.42857	40
ATCTCGT	180	0.0	32.500004	37
ATCTCCG	180	0.0	32.500004	10
CGTATGC	180	0.0	32.500004	41
CTCGTAT	180	0.0	32.500004	39
CTCCGAG	180	0.0	32.500004	12
CACGAGA	180	0.0	32.500004	21
CCCACGA	180	0.0	32.500004	19
TCTCGTA	180	0.0	32.500004	38