##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_44.34100000006ada.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 85552 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.329799420235645 38.0 38.0 38.0 32.0 38.0 2 36.403579109781184 38.0 38.0 38.0 32.0 38.0 3 36.35190293622592 38.0 38.0 38.0 32.0 38.0 4 36.430965962221805 38.0 38.0 38.0 32.0 38.0 5 36.4808888161586 38.0 38.0 38.0 32.0 38.0 6 38.4014517486441 40.0 38.0 40.0 38.0 40.0 7 38.25347157284459 40.0 38.0 40.0 38.0 40.0 8 38.212467271367125 40.0 38.0 40.0 38.0 40.0 9 38.25453525341313 40.0 38.0 40.0 38.0 40.0 10 38.22150271180101 40.0 38.0 40.0 38.0 40.0 11 38.17094866280157 40.0 38.0 40.0 38.0 40.0 12 38.094632504207965 40.0 38.0 40.0 38.0 40.0 13 38.135566672900694 40.0 38.0 40.0 38.0 40.0 14 38.082359266878626 40.0 38.0 40.0 38.0 40.0 15 38.11204881241818 40.0 38.0 40.0 38.0 40.0 16 38.0988989152796 40.0 38.0 40.0 38.0 40.0 17 38.12537404151861 40.0 38.0 40.0 38.0 40.0 18 38.121668692724896 40.0 38.0 40.0 38.0 40.0 19 38.10656676641107 40.0 38.0 40.0 38.0 40.0 20 38.08649710117823 40.0 38.0 40.0 38.0 40.0 21 38.100921077239576 40.0 38.0 40.0 38.0 40.0 22 38.08992191883299 40.0 38.0 40.0 38.0 40.0 23 38.0894076117449 40.0 38.0 40.0 38.0 40.0 24 38.094667570600336 40.0 38.0 40.0 38.0 40.0 25 38.09967037591173 40.0 38.0 40.0 38.0 40.0 26 38.039753600149616 40.0 38.0 40.0 38.0 40.0 27 38.00985365625584 40.0 38.0 40.0 32.0 40.0 28 37.99609594164952 40.0 38.0 40.0 32.0 40.0 29 37.96887273237329 40.0 38.0 40.0 32.0 40.0 30 37.96818309332336 40.0 38.0 40.0 32.0 40.0 31 37.949901814101366 40.0 38.0 40.0 32.0 40.0 32 37.92799700766785 40.0 38.0 40.0 32.0 40.0 33 37.91242752945577 40.0 38.0 40.0 32.0 40.0 34 37.923496820647095 40.0 38.0 40.0 32.0 40.0 35 37.917371890779876 40.0 38.0 40.0 32.0 40.0 36 37.898377594913036 40.0 38.0 40.0 32.0 40.0 37 37.8872849261268 40.0 38.0 40.0 32.0 40.0 38 37.87701047316252 40.0 38.0 40.0 32.0 40.0 39 37.854006919768096 40.0 38.0 40.0 32.0 40.0 40 37.82214325790163 40.0 38.0 40.0 32.0 40.0 41 37.85682391995511 40.0 38.0 40.0 32.0 40.0 42 37.84334673648775 40.0 38.0 40.0 32.0 40.0 43 37.838834860669536 40.0 38.0 40.0 32.0 40.0 44 37.79930334767159 40.0 38.0 40.0 32.0 40.0 45 37.7786843089583 40.0 38.0 40.0 32.0 40.0 46 37.72110529268749 40.0 38.0 40.0 32.0 40.0 47 37.71710772395736 40.0 38.0 40.0 32.0 40.0 48 37.6501075369366 40.0 38.0 40.0 32.0 40.0 49 37.62064007854872 40.0 38.0 40.0 32.0 40.0 50 37.60874088273798 40.0 38.0 40.0 32.0 40.0 51 37.16152749205162 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 1.0194149044216303 2201 2 0.6048365300109424 2201 3 0.852306414927952 2201 4 0.5935910485265765 2201 5 0.7134984991183373 2201 6 0.43376884971223717 2201 7 0.6311634092581215 2201 8 0.519586028204202 2201 9 0.20355735220385895 2201 10 0.33998939411850415 2201 11 0.33238531227270585 2201 12 0.44118780608268793 2201 13 0.4251420929409093 2201 14 0.5935456730459876 2201 15 0.70142903752199 2201 16 0.7082371071892268 2201 17 0.6916170116124576 2201 18 0.5517147873699457 2201 19 0.41152104721579263 2201 20 0.4239009392057511 2201 21 0.7032325156044479 2201 22 0.5762655282487685 2201 23 -0.01851686141137776 2201 24 -0.06818823872200852 2201 25 0.2324594135464153 2201 26 0.5514791087287776 2201 27 -0.07332828351670173 2201 28 0.21160531110560754 2201 29 -0.01944257358920254 2201 30 0.03218402392263897 2201 31 0.37943122276857366 2201 32 -0.28218807924830003 2201 33 0.40777285900346527 2201 34 0.5377861898522269 2201 35 0.2132443677441529 2201 36 0.14445578253063474 2201 37 0.7163941872600361 2201 38 0.4747077059327509 2201 39 0.3707540993062466 2201 40 0.518239610843743 2201 41 0.24599454445771585 2201 42 0.04373162384236906 2201 43 0.877816704950753 2201 44 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scores pass #Quality Count 15 3.0 16 3.0 17 0.0 18 0.0 19 1.0 20 1.0 21 9.0 22 26.0 23 54.0 24 116.0 25 211.0 26 328.0 27 497.0 28 643.0 29 950.0 30 1150.0 31 1452.0 32 1746.0 33 2138.0 34 2631.0 35 3397.0 36 4527.0 37 7090.0 38 13623.0 39 44956.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.750038002362 22.830649781924908 14.630324715566937 25.788987500146167 2 12.518994739918178 22.10169491525424 44.868497954412625 20.510812390414962 3 17.87217131101552 26.325509631569105 31.54689545539555 24.255423602019825 4 12.851832803441182 20.024078922760427 38.602253600149616 28.521834673648776 5 12.827286328782495 38.227043201795404 37.14699831681317 11.79867215260894 6 28.408453338320555 40.82312511688798 18.18075556386759 12.587665980923882 7 25.080652702449974 33.38554329530578 22.392229287450906 19.141574714793343 8 21.908313072751078 42.81255844398728 20.451888909668973 14.827239573592669 9 23.88255096315691 18.116467177856745 22.385216008976997 35.61576585000935 10 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23 18.878576772021695 30.86543856368057 29.355245932298484 20.90073873199925 24 22.694969141574713 29.038479521226858 30.559192070319806 17.707359266878623 25 19.764587619225736 31.175191696278286 30.1570974378156 18.90312324668038 26 19.88404577386588 30.788652382789213 30.58058935605662 18.746712487288285 27 18.90312324668038 31.067654759678327 29.932672526650457 20.096549466990837 28 19.148587993267252 29.308490742472415 28.72054423040958 22.822377033850756 29 20.963858238264446 28.631709369740044 27.907003927435948 22.497428464559565 30 18.8523536219009 30.802230300763306 29.013781575471363 21.33163450186443 31 21.166074434262203 28.615345053300917 30.367495792032916 19.851084720403964 32 19.409248176547596 30.10683560875257 30.770759304282773 19.713156910417055 33 18.86221245558257 29.8590331026744 30.87478960164578 20.40396484009725 34 19.791471853375725 28.964840097250793 31.15882737983916 20.08486066953432 35 21.099448288760055 29.863708621657004 29.738638488872265 19.298204600710676 36 18.458948943332707 31.605339442678137 30.570880867776324 19.36483074621283 37 19.7412100243127 30.06592481765476 28.283383205535817 21.909481952496726 38 20.23330839723209 30.345287076865535 29.763184963530954 19.658219562371425 39 21.465307649149057 28.088180288011973 28.857303160650833 21.589208902188144 40 22.111698148494483 27.905835047690292 31.001028614176175 18.98143818963905 41 20.775668599214512 27.053721713110157 30.77426594351973 21.3963437441556 42 21.3369802807682 29.457282790382344 30.17498334326893 19.03075358558053 43 18.38764727884795 29.418365438563683 32.6070693847017 19.586917897886664 44 18.045165513371984 30.39671778567421 30.247101178230785 21.31101552272302 45 18.92766972133907 28.951982420048626 30.85725640546101 21.2630914531513 46 21.05269309893398 27.388021320366562 29.494342622031045 22.064942958668414 47 21.016457826818776 27.16944080792968 28.74275294557696 23.071348419674585 48 18.65765850009351 27.991163269122872 29.136665419861608 24.214512810922013 49 21.690901440059847 27.86959977557509 29.17290069197681 21.266598092388257 50 19.28651580325416 29.638114830746215 30.17346175425472 20.901907611744903 51 18.462149904147378 28.67279188291953 30.084864637396546 22.780193575536543 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 252.0 1 134.0 2 16.0 3 16.5 4 17.0 5 23.0 6 29.0 7 23.0 8 17.0 9 21.0 10 25.0 11 44.5 12 64.0 13 103.0 14 142.0 15 182.0 16 222.0 17 207.5 18 193.0 19 170.0 20 147.0 21 146.5 22 146.0 23 142.5 24 139.0 25 214.5 26 582.0 27 874.0 28 1808.0 29 2742.0 30 4459.0 31 6176.0 32 7988.0 33 9800.0 34 10392.5 35 10985.0 36 10018.0 37 9051.0 38 7659.0 39 6267.0 40 5461.5 41 4656.0 42 4233.5 43 3811.0 44 3728.5 45 3646.0 46 3635.5 47 3625.0 48 3640.0 49 3655.0 50 3649.0 51 3643.0 52 4126.0 53 4609.0 54 3656.5 55 2704.0 56 2448.5 57 2193.0 58 1927.5 59 1662.0 60 1476.0 61 1290.0 62 1114.0 63 938.0 64 792.0 65 646.0 66 499.0 67 352.0 68 296.5 69 241.0 70 182.0 71 123.0 72 102.0 73 81.0 74 66.5 75 35.5 76 19.0 77 14.5 78 10.0 79 6.0 80 2.0 81 1.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03623527211520479 2 0.0023377594913035348 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0011688797456517674 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0011688797456517674 17 0.0 18 0.003506639236955302 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0011688797456517674 27 0.0 28 0.0 29 0.0 30 0.003506639236955302 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0011688797456517674 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0046755189826070695 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 85552.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.6638185611977 #Duplication Level Percentage of deduplicated Percentage of total 1 88.160618868738 68.4689030806457 2 7.866954732398892 12.219554899323665 3 1.9533950347375064 4.551243526685946 4 0.787721794458617 2.4470993008614044 5 0.3329170243886999 1.292780368902891 6 0.23110605287003586 1.0769147135095816 7 0.15938187931508507 0.8664743749948633 8 0.09133285322940404 0.5674606513507947 9 0.048565817826887424 0.33946261775852093 >10 0.3393130984323105 4.269508102893395 >50 0.016623531389314 0.8225800869165703 >100 0.010560649035483337 1.3966496774430905 >500 0.0 0.0 >1k 0.0015086631797796143 1.6813685987136169 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC 1435 1.6773424350102861 RNA PCR Primer, Index 46 (95% over 21bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 247 0.28871329717598654 No Hit GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA 191 0.22325603141948755 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT 186 0.21741163269122873 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA 180 0.21039835421731812 No Hit TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT 146 0.17065644286515805 RNA PCR Primer, Index 18 (95% over 21bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 123 0.14377220871516738 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC 119 0.1390966897325603 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAG 90 0.10519917710865906 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0011688797456517674 3 0.0 0.0 0.0023377594913035348 4 0.0 0.0 0.0023377594913035348 5 0.0 0.0 0.0023377594913035348 6 0.0 0.0 0.0023377594913035348 7 0.0 0.0 0.0023377594913035348 8 0.0 0.0 0.0023377594913035348 9 0.0 0.0 0.0023377594913035348 10 0.0 0.0 0.003506639236955302 11 0.0 0.0 0.003506639236955302 12 0.0 0.0 0.003506639236955302 13 0.0 0.0 0.003506639236955302 14 0.0 0.0 0.003506639236955302 15 0.0 0.0 0.003506639236955302 16 0.0 0.0 0.003506639236955302 17 0.0 0.0 0.003506639236955302 18 0.0 0.0 0.003506639236955302 19 0.0 0.0 0.0046755189826070695 20 0.0 0.0 0.005844398728258837 21 0.0 0.0 0.005844398728258837 22 0.0 0.0 0.005844398728258837 23 0.0 0.0 0.010519917710865906 24 0.0 0.0 0.014026556947821208 25 0.0 0.0 0.01753319618477651 26 0.0 0.0 0.023377594913035347 27 0.0 0.0 0.024546474658687114 28 0.0 0.0 0.03974191135216009 29 0.0 0.0 0.05259958855432953 30 0.0 0.0 0.07480830372171311 31 0.0 0.0 0.20338507574340753 32 0.0 0.0 0.34481952496727136 33 0.0 0.0 0.48742285393678697 34 0.0 0.0 0.6744436132410697 35 0.0 0.0 0.8544510940714419 36 0.0 0.0 1.10459135964092 37 0.0 0.0 1.386291378342996 38 0.0 0.0 1.7498129792406958 39 0.0 0.0 2.160089769964466 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTCTAA 30 1.1311134E-4 37.500004 1 GTATAGG 25 0.0020962488 36.0 1 GCCGTCT 45 8.5754436E-7 35.000004 45 ATGCCGT 170 0.0 34.411762 44 CATTCCG 165 0.0 34.09091 29 ATTCCGA 165 0.0 34.09091 30 CGAGACC 165 0.0 34.09091 23 CCGAATC 165 0.0 34.09091 33 GCCCACG 175 0.0 33.42857 18 TATGCCG 175 0.0 33.42857 43 CCGAGCC 175 0.0 33.42857 14 TCGTATG 175 0.0 33.42857 40 ATCTCGT 180 0.0 32.500004 37 ATCTCCG 180 0.0 32.500004 10 CGTATGC 180 0.0 32.500004 41 CTCGTAT 180 0.0 32.500004 39 CTCCGAG 180 0.0 32.500004 12 CACGAGA 180 0.0 32.500004 21 CCCACGA 180 0.0 32.500004 19 TCTCGTA 180 0.0 32.500004 38 >>END_MODULE