FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_43.34100000006acd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_43.34100000006acd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70742
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC8551.2086172288032568RNA PCR Primer, Index 46 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1950.27564954341126907No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA1200.16963048825308868No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.13570439060247094No Hit
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT930.13146362839614373RNA PCR Primer, Index 18 (95% over 21bp)
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT880.12439569138559838No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC770.10884622996239857No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACACT253.8536564E-545.04
CCCACGA1200.041.24999619
ATCTCGT1150.041.0869637
CGTATGC1150.041.0869641
CTCGTAT1150.041.0869639
TATGCCG1150.041.0869643
TCTCGTA1150.041.0869638
TCGTATG1150.041.0869640
ACGAGAC1150.041.0869622
ACCATTC1100.040.90909227
ATTCCGA1100.040.90909230
AATCTCG1100.040.90909236
CCGAATC1100.040.90909233
GCCCACG1200.039.37499618
CATTCCG1200.039.37499629
ATGCCGT1200.039.37499644
CCACGAG1200.039.37499620
TGCCGTC1200.039.37499645
TCCGAAT1150.039.13043632
GACCATT1150.039.13043626
Produced by FastQC (version 0.11.2)