##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_42.34100000006ab0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 103678 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.28455410019483 38.0 38.0 38.0 32.0 38.0 2 36.30425934142248 38.0 38.0 38.0 32.0 38.0 3 36.303516657342925 38.0 38.0 38.0 32.0 38.0 4 36.41421516618762 38.0 38.0 38.0 32.0 38.0 5 36.43850190011381 38.0 38.0 38.0 32.0 38.0 6 38.32215127606628 40.0 38.0 40.0 38.0 40.0 7 38.24222110766026 40.0 38.0 40.0 38.0 40.0 8 38.216034259920136 40.0 38.0 40.0 38.0 40.0 9 38.260817145392465 40.0 38.0 40.0 38.0 40.0 10 38.211028376318986 40.0 38.0 40.0 38.0 40.0 11 38.19106271340111 40.0 38.0 40.0 38.0 40.0 12 38.10875981403962 40.0 38.0 40.0 38.0 40.0 13 38.13493701653196 40.0 38.0 40.0 38.0 40.0 14 38.0981982677135 40.0 38.0 40.0 38.0 40.0 15 38.07436486043326 40.0 38.0 40.0 38.0 40.0 16 38.07660255791971 40.0 38.0 40.0 38.0 40.0 17 38.063591118655836 40.0 38.0 40.0 32.0 40.0 18 38.07972761820251 40.0 38.0 40.0 38.0 40.0 19 38.08972009490924 40.0 38.0 40.0 38.0 40.0 20 38.100850710854765 40.0 38.0 40.0 38.0 40.0 21 38.10722621964158 40.0 38.0 40.0 38.0 40.0 22 38.086209224714985 40.0 38.0 40.0 38.0 40.0 23 38.08833117922799 40.0 38.0 40.0 38.0 40.0 24 38.088900248847395 40.0 38.0 40.0 38.0 40.0 25 38.07592739057466 40.0 38.0 40.0 38.0 40.0 26 38.01386986631687 40.0 38.0 40.0 32.0 40.0 27 37.99646019406239 40.0 38.0 40.0 32.0 40.0 28 37.973543085321865 40.0 38.0 40.0 32.0 40.0 29 37.93589768321148 40.0 38.0 40.0 32.0 40.0 30 37.96867223518972 40.0 38.0 40.0 32.0 40.0 31 37.95486024035957 40.0 38.0 40.0 32.0 40.0 32 37.937691699299755 40.0 38.0 40.0 32.0 40.0 33 37.895908485889 40.0 38.0 40.0 32.0 40.0 34 37.92343602307143 40.0 38.0 40.0 32.0 40.0 35 37.9162888944617 40.0 38.0 40.0 32.0 40.0 36 37.88026389397944 40.0 38.0 40.0 32.0 40.0 37 37.880379636952874 40.0 38.0 40.0 32.0 40.0 38 37.87172784968846 40.0 38.0 40.0 32.0 40.0 39 37.83748722004668 40.0 38.0 40.0 32.0 40.0 40 37.804037500723396 40.0 38.0 40.0 32.0 40.0 41 37.828372460888524 40.0 38.0 40.0 32.0 40.0 42 37.839261945639386 40.0 38.0 40.0 32.0 40.0 43 37.8306583846139 40.0 38.0 40.0 32.0 40.0 44 37.78968537201721 40.0 38.0 40.0 32.0 40.0 45 37.76067246667567 40.0 38.0 40.0 32.0 40.0 46 37.708964293292695 40.0 38.0 40.0 32.0 40.0 47 37.689287987808406 40.0 38.0 40.0 32.0 40.0 48 37.65612762591871 40.0 38.0 40.0 32.0 40.0 49 37.621182893188525 40.0 38.0 40.0 32.0 40.0 50 37.61054418488011 40.0 38.0 40.0 32.0 40.0 51 37.129034124886665 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.07086479755572839 2201 2 0.2628395650902462 2201 3 -0.08040594126447331 2201 4 0.06221537784483644 2201 5 0.1667062498090175 2201 6 0.27841899752500154 2201 7 0.5857595572263818 2201 8 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25 257.0 26 389.0 27 602.0 28 888.0 29 1129.0 30 1388.0 31 1808.0 32 2171.0 33 2605.0 34 3238.0 35 4019.0 36 5625.0 37 8547.0 38 16256.0 39 54502.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.930337794158795 19.014308732861842 13.008114392674855 28.047239080304507 2 15.191558316293067 20.709711022801805 39.49805162236197 24.600679038543152 3 19.29242462238855 23.010667644051775 29.458515789270628 28.23839194428905 4 13.754123343428692 17.45596944385501 35.823414803526305 32.96649240918999 5 14.751441964544068 36.11855938579062 34.953413453191615 14.176585196473699 6 32.717645016300466 36.9046470803835 16.797199020042825 13.580508883273213 7 28.57983371592816 31.387565346553753 20.74306988946546 19.289531048052623 8 24.45166766334227 37.411022589170315 20.066938019637725 18.07037172784969 9 24.632998321726884 17.531202376589057 20.6813403036324 37.15445899805166 10 16.1143154767646 29.037018461004266 32.3424448774089 22.506221184822238 11 33.77508994280313 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23.39069040683655 27.141727270973593 27.878624201855747 21.588958120334112 25 22.285345010513318 27.966395956712127 27.789887922220725 21.95837111055383 26 22.387584637049326 27.71369046470804 28.242250043403615 21.65647485483902 27 22.03842666718108 28.40043210710083 27.542005054109843 22.01913617160825 28 21.55809332741758 27.80917841779355 27.26229286830379 23.370435386485077 29 22.38372653793476 27.544898628445765 27.01151642585698 23.059858407762494 30 21.770164168451107 28.347512394621603 27.55367787487702 22.328645562050273 31 22.823549836995312 26.988367831169587 27.931673064681036 22.256409267154073 32 21.898570574278054 27.528501707208857 28.191130230135613 22.381797488377476 33 22.010455448600474 27.34524199926696 27.849688458496498 22.794614093636064 34 22.315245278651208 27.354887247053377 27.859333706282914 22.470533768012498 35 22.983660950249813 27.60277011516426 27.564189124018597 21.849379810567335 36 21.998881151256775 27.977005729277188 28.1467620903181 21.87735102914794 37 22.20432492910743 27.725264762051737 27.161017766546426 22.909392542294412 38 21.709523717664307 27.653889928432264 27.98568645228496 22.650899901618473 39 22.616177009587375 26.907347749763694 27.407936109878662 23.06853913077027 40 23.073361754663477 26.46945350026042 28.498813634522268 21.95837111055383 41 22.985589999807097 26.20228013657671 27.545863153224403 23.26626671039179 42 23.145701113061595 26.899631551534558 27.698258068249775 22.256409267154073 43 21.79825999729933 26.955573988695768 28.586585389378655 22.659580624626248 44 21.959335635332472 27.509211211636025 27.86319180539748 22.66826134763402 45 21.6815524990837 27.109897953278423 28.25768243986188 22.950867107776 46 22.741565230810778 26.087501687918362 27.690541870020642 23.480391211250218 47 22.273985551279456 26.330815899379807 27.692738022898038 23.7024605264427 48 21.466463473446634 27.05492004089585 27.587337718705996 23.891278766951523 49 23.193927351993672 26.283300217982603 27.48509809216999 23.03767433785374 50 21.633326260151623 27.158124192210497 28.010764096529638 23.19778545110824 51 21.490441190655325 26.899704844030325 27.535351197021434 24.07450276829292 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 141.0 1 76.5 2 12.0 3 12.0 4 12.0 5 17.5 6 23.0 7 18.0 8 13.0 9 15.5 10 18.0 11 36.0 12 54.0 13 96.0 14 138.0 15 163.5 16 189.0 17 199.0 18 209.0 19 201.0 20 193.0 21 188.0 22 183.0 23 202.5 24 222.0 25 279.5 26 554.5 27 772.0 28 1174.5 29 1577.0 30 2348.5 31 3120.0 32 3887.5 33 4655.0 34 5436.0 35 6217.0 36 6550.5 37 6884.0 38 6962.5 39 7041.0 40 7012.5 41 6984.0 42 7018.5 43 7053.0 44 7190.5 45 7328.0 46 7288.5 47 7249.0 48 7354.0 49 7459.0 50 7215.5 51 6972.0 52 7149.5 53 7327.0 54 6435.5 55 5544.0 56 4958.0 57 4372.0 58 3961.5 59 3551.0 60 3143.5 61 2736.0 62 2317.0 63 1898.0 64 1589.0 65 1280.0 66 1076.0 67 872.0 68 665.5 69 459.0 70 361.5 71 264.0 72 213.0 73 162.0 74 129.0 75 67.5 76 39.0 77 26.5 78 14.0 79 9.0 80 4.0 81 2.5 82 1.0 83 1.5 84 2.0 85 1.5 86 1.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.033758367252454714 2 0.0019290495572831267 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 9.645247786415634E-4 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0038580991145662534 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0038580991145662534 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 9.645247786415634E-4 48 0.0 49 0.0 50 0.0 51 0.0038580991145662534 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 103678.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.92435972786575 #Duplication Level Percentage of deduplicated Percentage of total 1 91.15344651272271 80.146084216194 2 7.1137430479657775 12.509426055218942 3 1.1500056585610061 3.0334053363719713 4 0.2730039090237011 0.960147756164537 5 0.11026375186462675 0.4847434891944784 6 0.06457283088428208 0.3406514886789753 7 0.03747771421700847 0.23066428186160806 8 0.013611502797066391 0.09574261346928928 9 0.020913501461432126 0.16549256030977838 >10 0.053060482932810245 0.6801736654978828 >50 0.007710287178745426 0.47028914161626373 >100 0.0010954088759264871 0.12905958623511707 >500 0.0010953915148819724 0.7541198091871277 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC 783 0.7552229016763441 RNA PCR Primer, Index 46 (95% over 21bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 134 0.12924632033796948 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 9.645247786415633E-4 18 0.0 0.0 9.645247786415633E-4 19 0.0 0.0 9.645247786415633E-4 20 0.0 0.0 9.645247786415633E-4 21 0.0 0.0 9.645247786415633E-4 22 0.0 0.0 9.645247786415633E-4 23 0.0 0.0 0.0019290495572831265 24 0.0 0.0 0.0019290495572831265 25 0.0 0.0 0.0019290495572831265 26 0.0 0.0 0.00289357433592469 27 0.0 0.0 0.00289357433592469 28 0.0 0.0 0.01157429734369876 29 0.0 0.0 0.022184069908755957 30 0.0 0.0 0.04919076371071973 31 0.0 0.0 0.14274966723895136 32 0.0 0.0 0.2575281158972974 33 0.0 0.0 0.3800227627847759 34 0.0 0.0 0.5131271822373117 35 0.0 0.0 0.6539477999189799 36 0.0 0.0 0.815023437952121 37 0.0 0.0 1.0706225042921353 38 0.0 0.0 1.4014545033661914 39 0.0 0.0 1.742896275005305 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGAGCC 105 0.0 44.99566 14 CGAGCCC 105 0.0 44.99566 15 CTCGTAT 110 0.0 42.9504 39 TATGCCG 110 0.0 42.9504 43 ATGCCGT 110 0.0 42.9504 44 TGCCGTC 110 0.0 42.9504 45 CATTCCG 95 0.0 42.648037 29 ATTCCGA 95 0.0 42.648037 30 CGAATCT 95 0.0 42.627464 34 CCGAATC 95 0.0 42.627464 33 CCACGAG 120 0.0 41.24602 20 CACGAGA 115 0.0 41.082993 21 CCCACGA 115 0.0 41.082993 19 TCTCGTA 115 0.0 41.082993 38 TCCGAAT 100 0.0 40.496094 32 TTCCGAA 100 0.0 40.496094 31 ATCTCGT 120 0.0 39.3712 37 ATCTCCG 120 0.0 39.3712 10 GCCCACG 120 0.0 39.3712 18 CGTATGC 120 0.0 39.3712 41 >>END_MODULE