FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_42.34100000006ab0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_42.34100000006ab0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103678
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC7830.7552229016763441RNA PCR Primer, Index 46 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1340.12924632033796948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGCC1050.044.9956614
CGAGCCC1050.044.9956615
CTCGTAT1100.042.950439
TATGCCG1100.042.950443
ATGCCGT1100.042.950444
TGCCGTC1100.042.950445
CATTCCG950.042.64803729
ATTCCGA950.042.64803730
CGAATCT950.042.62746434
CCGAATC950.042.62746433
CCACGAG1200.041.2460220
CACGAGA1150.041.08299321
CCCACGA1150.041.08299319
TCTCGTA1150.041.08299338
TCCGAAT1000.040.49609432
TTCCGAA1000.040.49609431
ATCTCGT1200.039.371237
ATCTCCG1200.039.371210
GCCCACG1200.039.371218
CGTATGC1200.039.371241