FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_41.34100000006aa4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_41.34100000006aa4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95029
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC5310.5587767944522198RNA PCR Primer, Index 46 (95% over 21bp)
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA1010.10628334508413222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAT750.042.032
CCGAATC750.042.033
CTCGTAT800.039.37539
TTCCGAA800.039.37531
CATTCCG800.039.37529
CGAATCT800.039.37534
ATCTCGT850.037.05882337
ATCTCCG850.037.05882310
ACCATTC850.037.05882327
GAATCTC850.037.05882335
GACCATT850.037.05882326
ATTCCGA850.037.05882330
AATCTCG850.037.05882336
TCGTATG850.037.05882340
CCACGAG850.037.05882320
GCCCACG900.035.018
CGTATGC900.035.041
CACGAGA900.035.021
TATGCCG900.035.043
CCCACGA900.035.019