##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_40.34100000006a97.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23036 Sequences flagged as poor quality 0 Sequence length 51 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.30139781212016 38.0 38.0 38.0 32.0 38.0 2 36.41943045667651 38.0 38.0 38.0 32.0 38.0 3 36.3152022920646 38.0 38.0 38.0 32.0 38.0 4 36.38830526133009 38.0 38.0 38.0 32.0 38.0 5 36.401284945303004 38.0 38.0 38.0 32.0 38.0 6 38.339164785553045 40.0 38.0 40.0 38.0 40.0 7 38.16040111130405 40.0 38.0 40.0 38.0 40.0 8 38.188661225907275 40.0 38.0 40.0 38.0 40.0 9 38.23411182496961 40.0 38.0 40.0 38.0 40.0 10 38.127582913700294 40.0 38.0 40.0 38.0 40.0 11 38.078138565723215 40.0 38.0 40.0 38.0 40.0 12 37.988192394512936 40.0 38.0 40.0 32.0 40.0 13 38.06407362389304 40.0 38.0 40.0 38.0 40.0 14 37.925030387220005 40.0 38.0 40.0 32.0 40.0 15 37.877061989928805 40.0 38.0 40.0 32.0 40.0 16 37.85032123632575 40.0 38.0 40.0 32.0 40.0 17 37.83721132140997 40.0 38.0 40.0 32.0 40.0 18 37.82644556346588 40.0 38.0 40.0 32.0 40.0 19 37.9180413266192 40.0 38.0 40.0 32.0 40.0 20 37.90302135787463 40.0 38.0 40.0 32.0 40.0 21 38.00525264802917 40.0 38.0 40.0 32.0 40.0 22 38.04262892863344 40.0 38.0 40.0 32.0 40.0 23 38.026002778260114 40.0 38.0 40.0 32.0 40.0 24 37.95385483590901 40.0 38.0 40.0 32.0 40.0 25 37.89281993401632 40.0 38.0 40.0 32.0 40.0 26 37.77439659663136 40.0 38.0 40.0 32.0 40.0 27 37.74318458065636 40.0 38.0 40.0 32.0 40.0 28 37.78412050703247 40.0 38.0 40.0 32.0 40.0 29 37.754905365514844 40.0 38.0 40.0 32.0 40.0 30 37.71110435839555 40.0 38.0 40.0 32.0 40.0 31 37.67859003299184 40.0 38.0 40.0 32.0 40.0 32 37.66456850147595 40.0 38.0 40.0 32.0 40.0 33 37.55621635700643 40.0 38.0 40.0 32.0 40.0 34 37.57852925855183 40.0 38.0 40.0 32.0 40.0 35 37.641300573016146 40.0 38.0 40.0 32.0 40.0 36 37.55751866643514 40.0 38.0 40.0 32.0 40.0 37 37.46948254905365 40.0 38.0 40.0 32.0 40.0 38 37.50503559645772 40.0 38.0 40.0 32.0 40.0 39 37.30061642646293 40.0 38.0 40.0 32.0 40.0 40 37.27439659663136 40.0 38.0 40.0 32.0 40.0 41 37.305044278520576 40.0 38.0 40.0 32.0 40.0 42 37.26046188574405 40.0 38.0 40.0 32.0 40.0 43 37.26354401805869 40.0 38.0 40.0 32.0 40.0 44 37.126150373328706 40.0 38.0 40.0 32.0 40.0 45 37.03993748914742 40.0 38.0 40.0 32.0 40.0 46 36.89377496093072 40.0 38.0 40.0 32.0 40.0 47 36.82002083695086 40.0 38.0 40.0 32.0 40.0 48 36.66877930196215 40.0 38.0 40.0 27.0 40.0 49 36.52617641951728 40.0 38.0 40.0 27.0 40.0 50 36.46205938530995 40.0 38.0 40.0 27.0 40.0 51 35.98524049314117 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.10450871033145859 2201 2 -0.3179362375564523 2201 3 -0.5937545472608008 2201 4 -0.3657048779272145 2201 5 0.06562294597384266 2201 6 1.0157652743450782 2201 7 0.8321378830436785 2201 8 0.8917526661487258 2201 9 -0.8749808740061198 2201 10 -0.35776981389877704 2201 11 -0.9812903457620976 2201 12 0.21867432889686 2201 13 -1.0070009100716248 2201 14 -0.9689558536028215 2201 15 -1.313889368591603 2201 16 -1.0613107148438914 2201 17 -2.1451529924441317 2201 18 -1.6848959373703352 2201 19 -0.2755695139893106 2201 20 -1.0668981766914314 2201 21 -0.6237510186583322 2201 22 -0.7630747510312403 2201 23 -1.079044120092739 2201 24 -1.0227520330198558 2201 25 -1.814744290229008 2201 26 -1.075974850106391 2201 27 -2.1032474415384854 2201 28 -0.6688503018996741 2201 29 -1.4372611178193182 2201 30 -0.0928094510204005 2201 31 -0.6504572987415074 2201 32 -0.32257875479741926 2201 33 -0.10642885455218476 2201 34 0.541657437428718 2201 35 -0.7754747649897524 2201 36 -1.3243380755714327 2201 37 -2.6642503356194993 2201 38 -0.41060289771873215 2201 39 -0.5961228908438159 2201 40 -1.0514133512146202 2201 41 -0.919616249898418 2201 42 -0.7269463476769502 2201 43 -0.573752069940511 2201 44 0.49221576568563563 2201 45 -0.5773304435875488 2201 46 -0.9244355009834564 2201 47 -1.4100169268206386 2201 48 -2.9198319410137827 2201 49 -1.5280160755023715 2201 50 -1.199880236543109 2201 51 -1.752543400674405 1101 1 0.9100746230018757 1101 2 -0.23876957088978656 1101 3 1.2958287860725335 1101 4 1.2592951220727855 1101 5 0.9031229459738412 1101 6 1.0886819410117425 1101 7 0.77172121637701 1101 8 0.852169332815393 1101 9 1.1979357926605445 1101 10 1.3755635194345572 1101 11 1.0770429875712324 1101 12 1.3228409955635243 1101 13 0.7367490899283737 1101 14 1.0310441463971785 1101 15 1.7152772980750655 1101 16 1.7636892851561115 1101 17 1.8173470075558669 1101 18 1.677604062629662 1101 19 1.686930486010688 1101 20 1.00185182330857 1101 21 1.2824989813416678 1101 22 1.278591915635424 1101 23 0.07928921324059246 1101 24 1.0730813003134756 1101 25 1.185255709770992 1101 26 1.2802751498936118 1101 27 0.9821692251281817 1101 28 1.010316364766993 1101 29 0.5106555488473461 1101 30 0.8301072156462652 1101 31 0.9057927012584912 1101 32 0.7149212452025822 1101 33 0.8019044787811467 1101 34 0.8833241040953865 1101 35 0.3411919016769147 1101 36 0.05899525776190018 1101 37 1.248249664380502 1101 38 1.174813768947935 1101 39 1.6642937758228484 1101 40 1.4923366487853826 1101 41 1.299133750101582 1101 42 1.170970318989717 1101 43 1.3741645967261533 1101 44 1.2234657656856385 1101 45 2.270586223079121 1101 46 1.8526478323498736 1101 47 2.217066406512693 1101 48 0.9635013923195501 1101 49 0.5615672578309585 1101 50 1.8209530967902268 1101 51 2.6703732659922608 2202 1 0.7225746230018757 2202 2 0.35498042911021344 2202 3 0.3583287860725335 2202 4 -0.05320487792721451 2202 5 0.37187294597384124 2202 6 -0.09881805898825746 2202 7 0.33422121637700997 2202 8 0.383419332815393 2202 9 0.8541857926605445 2202 10 1.0005635194345572 2202 11 0.014542987571232402 2202 12 -0.1771590044364757 2202 13 0.23674908992837373 2202 14 0.9060441463971785 2202 15 1.3402772980750655 2202 16 1.2011892851561115 2202 17 0.5985970075558669 2202 18 0.208854062629662 2202 19 0.905680486010688 2202 20 0.50185182330857 2202 21 -0.31125101865833216 2202 22 0.966091915635424 2202 23 0.7667892132405925 2202 24 0.6355813003134756 2202 25 1.185255709770992 2202 26 1.4365251498936118 2202 27 0.4821692251281817 2202 28 -0.36468363523300695 2202 29 -1.114344451152654 2202 30 0.9551072156462652 2202 31 0.9370427012584912 2202 32 0.30867124520258216 2202 33 1.2394044787811467 2202 34 1.8208241040953865 2202 35 1.0911919016769147 2202 36 1.1839952577619002 2202 37 1.373249664380502 2202 38 1.643563768947935 2202 39 0.6017937758228484 2202 40 1.4610866487853826 2202 41 1.267883750101582 2202 42 1.108470318989717 2202 43 1.4054145967261533 2202 44 1.3172157656856385 2202 45 0.48933622307912117 2202 46 0.07139783234987362 2202 47 0.4670664065126928 2202 48 0.9322513923195501 2202 49 1.1240672578309585 2202 50 0.5709530967902268 2202 51 0.9203732659922608 1102 1 0.9100746230018757 1102 2 -0.051269570889786564 1102 3 0.1708287860725335 1102 4 1.0717951220727855 1102 5 0.5281229459738412 1102 6 0.8699319410117425 1102 7 1.05297121637701 1102 8 0.758419332815393 1102 9 1.3541857926605445 1102 10 1.3755635194345572 1102 11 1.0770429875712324 1102 12 0.6353409955635243 1102 13 1.1429990899283737 1102 14 0.31229414639717845 1102 15 1.2152772980750655 1102 16 1.4511892851561115 1102 17 1.6923470075558669 1102 18 1.427604062629662 1102 19 0.06193048601068796 1102 20 1.18935182330857 1102 21 0.8762489813416678 1102 22 -0.47140808436457604 1102 23 0.7042892132405925 1102 24 1.1355813003134756 1102 25 0.5602557097709919 1102 26 0.5302751498936118 1102 27 1.2321692251281817 1102 28 1.322816364766993 1102 29 1.041905548847346 1102 30 1.2988572156462652 1102 31 1.0307927012584912 1102 32 1.2149212452025822 1102 33 0.5831544787811467 1102 34 0.7895741040953865 1102 35 1.5286919016769147 1102 36 0.6527452577619002 1102 37 1.591999664380502 1102 38 1.643563768947935 1102 39 1.5080437758228484 1102 40 1.7110866487853826 1102 41 1.111633750101582 1102 42 1.295970318989717 1102 43 0.5304145967261533 1102 44 1.5359657656856385 1102 45 1.2705862230791212 1102 46 1.6026478323498736 1102 47 0.6858164065126928 1102 48 1.8072513923195501 1102 49 1.8115672578309585 1102 50 2.2897030967902268 1102 51 1.6703732659922608 2203 1 -0.18935719517994443 2203 2 0.41842739880718227 2203 3 0.2153363618301114 2203 4 -0.03237154459387881 2203 5 0.9201684005192945 2203 6 0.34909860767840684 2203 7 0.4412287921345879 2203 8 0.8493284237244865 2203 9 0.9432009441756932 2203 10 0.8149574588284949 2203 11 0.7399217754500214 2203 12 0.8853409955635243 2203 13 0.5899687868980692 2203 14 0.6977108130638427 2203 15 -0.15025300495523908 2203 16 0.5023256487924783 2203 17 0.7396954924043513 2203 18 1.0848010323266308 2203 19 0.9123092738894769 2203 20 1.1240109142176635 2203 21 0.9671580722507613 2203 22 0.115713127756635 2203 23 0.3179255768769593 2203 24 0.9802782700104444 2203 25 1.2761648006800854 2203 26 0.39675242262088517 2203 27 1.4149343766433304 2203 28 0.10690727385789955 2203 29 -1.982715663273865 2203 30 -1.0079609661719147 2203 31 -0.8110633593475711 2203 32 -0.4195484517671133 2203 33 0.5784196302962954 2203 34 -0.5219789262076446 2203 35 0.6972525077375238 2203 36 0.18778313654977552 2203 37 -0.26122003258919335 2203 38 0.9106092234933882 2203 39 1.0553922606713328 2203 40 1.2546472548459917 2203 41 0.09553526525309763 2203 42 0.5488112280806234 2203 43 0.07776308157463774 2203 44 0.8437309172007872 2203 45 0.6863059200488166 2203 46 0.40283722628926455 2203 47 -0.3615320783357916 2203 48 1.2741074529256124 2203 49 -0.14013728762358824 2203 50 0.9061803695175001 2203 51 0.28685053871953414 1103 1 -0.5997468055695521 1103 2 -1.4655552851754976 1103 3 -1.7889926424988971 1103 4 -1.1371334493557868 1103 5 -0.4200913397404449 1103 6 -0.03185377327397276 1103 7 0.008328359234155869 1103 8 0.3584193328153944 1103 9 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0.6304950822108708 1214 32 0.34884981663115155 1214 33 0.593571145447811 1214 34 0.36546696123824063 1214 35 0.4245252350102504 1214 36 -0.09576664700000492 1214 37 -0.3975836689528336 1214 38 0.07511138799555539 1214 39 0.7134009186799943 1214 40 -0.041889541690807164 1214 41 -0.08628291656508225 1214 42 0.28257746184686283 1214 43 0.8072003110118686 1214 44 0.5493586228284926 1214 45 -0.5582828245399298 1214 46 -0.5863402628882213 1214 47 -0.21001692682064288 1214 48 0.5944537732719297 1214 49 0.5005553530690534 1214 50 -0.5332135698764375 1214 51 -0.08111482924583413 1215 1 0.6526341468114012 1215 2 -0.0036505232707355617 1215 3 0.09195973845348249 1215 4 0.3485808363585008 1215 5 0.15609913645003104 1215 6 -0.17471091613111156 1215 7 0.22261407351986406 1215 8 -0.07491400051794272 1215 9 -0.5416475406727912 1215 10 0.6374682813393164 1215 11 0.0710906066188528 1215 12 -0.3765637663412349 1215 13 0.32157051849980434 1215 14 0.07866319401622945 1215 15 -1.323413178115409 1215 16 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1212 1 -0.1618730514167268 1212 2 0.030125777947425547 1212 3 -1.43018865578793 1212 4 -0.1796583662993072 1212 5 -0.7669351935610393 1212 6 -1.0850099194533769 1212 7 -0.5996450626927583 1212 8 0.2700472397921345 1212 9 0.16377881591635912 1212 10 -0.7523434573096281 1212 11 -0.8851663147543505 1212 12 0.04813169323794142 1212 13 -0.641884631001858 1212 14 -1.0154674815097948 1212 15 -0.03326921355284185 1212 16 -1.070613040425286 1212 17 -1.7684088063976233 1212 18 -1.1174540769052186 1212 19 -0.45696486282652415 1212 20 0.3354274047039212 1212 21 -0.5888672977281004 1212 22 0.5082430784261192 1212 23 -0.6503619495501027 1212 24 -0.012674513640014595 1212 25 -0.27986056929877634 1212 26 -0.3178353152226663 1212 27 -0.24820868184855982 1212 28 0.03502566709257593 1212 29 -0.14268747440846852 1212 30 0.47618279704161637 1212 31 -0.5178991592066282 1212 32 0.5797468265979333 1212 33 1.0780672694788223 1212 34 -0.28237357032321597 1212 35 -0.39718019134634375 1212 36 0.19271618799445633 1212 37 -0.19215731236368327 1212 38 0.6886219084828156 1212 39 0.2682181944274973 1212 40 0.8346331604132899 1212 41 -0.09403485454958371 1212 42 -0.2905122391498196 1212 43 0.10454250370289486 1212 44 0.15733204475540674 1212 45 0.8730571533116773 1212 46 -0.8515672839291994 1212 47 0.3791303600010636 1212 48 -0.5074288402385889 1212 49 -0.1528222770527634 1212 50 -2.064221321814422 1212 51 0.22342559157365827 1213 1 -0.4993805052032556 1213 2 0.26924324962303103 1213 3 -1.2578571113633643 1213 4 -0.9810894933118277 1213 5 -1.611300130949239 1213 6 -0.573978315398513 1213 7 -0.5960672451614499 1213 8 -0.8569652825692202 1213 9 -0.9262629252881709 1213 10 -2.150077506206472 1213 11 -0.8171877816595341 1213 12 -1.704402594180067 1213 13 -1.7480265510972686 1213 14 -2.097160981807953 1213 15 -2.2318380865403213 1213 16 -1.4254132789464506 1213 17 -3.0272042744954177 1213 18 -1.7310497835241847 1213 19 -1.6704413088611076 1213 20 -1.8643340741273278 1213 21 -1.2006740955814124 1213 22 -1.8912798792363716 1213 23 -1.5585312995799185 1213 24 -1.3996751099429332 1213 25 -1.7378212133059279 1213 26 -1.5170004911320305 1213 27 -2.9417089800000227 1213 28 -1.1867990198483938 1213 29 -0.8475175280757341 1213 30 -1.1081940664050194 1213 31 -0.8786624269466401 1213 32 -1.4661684983871623 1213 33 -2.932069880193211 1213 34 -2.1327015369302558 1213 35 -0.6011157906307787 1213 36 -2.280748331981691 1213 37 -1.2565580279271913 1213 38 -1.1593208464366782 1213 39 -2.3268921216130494 1213 40 -1.146285146086413 1213 41 -0.958077788359958 1213 42 -0.10386942460002757 1213 43 0.10573510954666432 1213 44 -0.3590662855964126 1213 45 -1.626048392305492 1213 46 -0.9398201163680753 1213 47 -2.748478465282176 1213 48 -2.4788062999881433 1213 49 -1.6459647934510926 1213 50 -2.5203930570559265 1213 51 -2.447415195546199 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 2.0 17 1.0 18 0.0 19 0.0 20 1.0 21 4.0 22 13.0 23 22.0 24 38.0 25 82.0 26 131.0 27 186.0 28 234.0 29 304.0 30 418.0 31 508.0 32 553.0 33 667.0 34 852.0 35 1010.0 36 1327.0 37 1989.0 38 3572.0 39 11122.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.224215051895598 25.16176662179181 15.0736092413254 28.54040908498719 2 9.372286855356833 25.295190137176593 49.036291022747 16.29623198471957 3 17.559472130578225 29.831567980552183 36.859697864212535 15.74926202465706 4 11.989928807084564 31.463795797881577 33.365167563813166 23.1811078312207 5 9.745615558256642 35.33165480118076 43.20628581350929 11.716443827053308 6 25.651154714360132 46.383920819586734 15.653759333217574 12.311165132835562 7 16.57839902760896 30.89077964924466 24.400937662788678 28.129883660357702 8 16.75204028477166 47.62111477687098 21.522833825316894 14.104011113040457 9 19.708282687966662 18.5665914221219 21.21896162528217 40.50616426462928 10 13.782774787289458 35.540024309776 32.783469352318114 17.893731550616426 11 24.02205531194373 21.786132939695218 33.63435071419268 20.557461034168366 12 19.434797707935406 23.88869595415871 31.394339295016493 25.28216704288939 13 26.94912311165133 20.689355790935927 34.780343809689185 17.581177287723563 14 16.057475256120853 23.57180065983678 26.007119291543674 34.3636047924987 15 20.47230421948255 31.311859697864215 22.54297621114777 25.672859871505473 16 25.417842413718255 29.884957673106143 28.947254178424135 15.749945734751467 17 15.658100364646641 40.98801875325577 29.896683451988192 13.457197430109394 18 27.19656190310818 23.88001389130057 34.82375412397985 14.09967008161139 19 17.03420732766105 29.835909011981247 26.293627365862125 26.83625629449557 20 16.604445216183365 26.06355270012155 30.0963708977253 27.235631185969787 21 11.725125889911443 31.906581003646465 26.27192220871679 30.0963708977253 22 14.546796318805349 43.00659836777218 26.36308386872721 16.083521444695258 23 12.675811772877235 28.99374891474214 29.961798923424205 28.36864038895642 24 25.659836777218263 31.350928980725822 31.520229206459454 11.469005035596458 25 15.601666956068764 38.873936447299876 29.649244660531345 15.875151936100018 26 21.731203333912138 31.203333912137527 33.165480118076054 13.899982635874283 27 15.167563813162008 40.15888175030387 30.73450251779823 13.939051918735892 28 11.425594721305782 28.724604966139957 30.96891821496788 28.880882097586387 29 15.362910227470048 28.83313075186664 40.766626150373334 15.037332870289982 30 23.063899982635874 27.83035249175204 32.25386351797187 16.851884007640216 31 18.392950164959192 28.125542628928635 29.058864386178158 24.422642819934016 32 23.69334953985067 31.524570237888522 30.391561034901894 14.390519187358915 33 12.094113561382185 43.47108873068241 31.44643167216531 12.988366035770099 34 12.406667824275047 32.783469352318114 31.845806563639524 22.96405625976732 35 11.264976558430282 33.48671644382705 31.37263413787116 23.875672859871504 36 10.778781038374719 33.54314985240493 42.40753603056086 13.270533078659488 37 11.490710192741796 41.634832436186834 30.013891300573015 16.86056607049835 38 12.26341378711582 34.00764021531516 39.28633443306129 14.442611564507727 39 13.934710887306826 29.701337037680155 31.27713144643167 25.086820628581354 40 14.72043757596805 29.731724257683627 41.426462927591594 14.121375238756729 41 15.74926202465706 28.737628060427156 31.481159923597847 24.031949991317937 42 23.715054696996006 31.789373155061647 31.29883660357701 13.19673554436534 43 11.646987324188226 31.43774960930717 42.55947213057822 14.355790935926377 44 10.340336864038896 41.29623198471957 32.731376975169304 15.632054176072236 45 12.506511547143601 29.466921340510506 41.643514499044976 16.38305261330092 46 22.97707935405452 27.635006077444004 31.32922382358048 18.058690744920995 47 14.694391387393646 26.970828268796666 30.76923076923077 27.565549574578917 48 12.441396075707587 27.773919083174164 30.543497134919257 29.241187706198996 49 23.632575099843724 27.3007466574058 31.007987497829486 18.058690744920995 50 11.325750998437229 30.76054870637264 38.81316200729293 19.100538287897205 51 11.192636651760518 29.561932878912867 30.97295185169105 28.272478617635567 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 164.0 1 98.5 2 33.0 3 54.0 4 75.0 5 76.0 6 77.0 7 88.0 8 99.0 9 83.0 10 67.0 11 119.0 12 171.0 13 336.5 14 502.0 15 557.5 16 613.0 17 465.0 18 317.0 19 289.0 20 261.0 21 355.5 22 450.0 23 321.5 24 193.0 25 204.0 26 346.5 27 478.0 28 373.0 29 268.0 30 511.0 31 754.0 32 1333.5 33 1913.0 34 2711.0 35 3509.0 36 3509.0 37 3509.0 38 3027.5 39 2546.0 40 2022.5 41 1499.0 42 1117.5 43 736.0 44 570.0 45 404.0 46 381.0 47 358.0 48 296.5 49 235.0 50 243.0 51 251.0 52 367.0 53 483.0 54 1474.0 55 2465.0 56 1282.5 57 100.0 58 93.5 59 87.0 60 82.0 61 77.0 62 62.0 63 47.0 64 34.5 65 22.0 66 25.5 67 29.0 68 21.5 69 14.0 70 10.5 71 7.0 72 4.5 73 2.0 74 3.0 75 2.5 76 1.0 77 1.0 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03906928286160792 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.013023094287202639 12 0.0 13 0.0 14 0.0 15 0.0 16 0.004341031429067546 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.013023094287202639 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 23036.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.791619203593534 #Duplication Level Percentage of deduplicated Percentage of total 1 81.80695591195999 45.64142532445237 2 10.725505722677683 11.96786662091193 3 3.5433004468636105 5.930594077660121 4 1.2076036538131913 2.69496652809655 5 0.6782304155099177 1.891978653721217 6 0.3986752851876031 1.3345643818242483 7 0.3192870987328403 1.246948096038596 8 0.24101517889278573 1.075730166645782 9 0.12430621887292939 0.6241720705197326 >10 0.7920572892241753 7.889089466148191 >50 0.06988404782798728 2.5776353221897184 >100 0.08541383623420667 7.243371863363376 >500 0.0 0.0 >1k 0.007764894203109698 9.881657428428149 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 2281 9.901892689703073 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 301 1.3066504601493314 No Hit TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT 216 0.93766278867859 Illumina Paired End PCR Primer 2 (95% over 21bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 155 0.6728598715054697 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 150 0.651154714360132 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 142 0.6164264629275916 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 134 0.5816982114950512 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 130 0.5643340857787811 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 121 0.5252648029171731 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 119 0.516582740059038 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 103 0.4471262371939573 No Hit CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC 101 0.43844417433582217 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 99 0.4297621114776871 No Hit GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 77 0.3342594200382011 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA 76 0.32991838860913353 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 67 0.2908491057475256 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT 58 0.25177982288591766 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 57 0.24743879145685013 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 57 0.24743879145685013 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA 53 0.23007466574057997 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 51 0.22139260288244486 No Hit GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCAT 50 0.21705157145337733 No Hit TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 50 0.21705157145337733 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 44 0.19100538287897206 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC 43 0.1866643514499045 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAACTGTCT 39 0.16930022573363432 No Hit GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 37 0.1606181628754992 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 35 0.15193610001736413 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC 34 0.14759506858829657 No Hit GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCAT 34 0.14759506858829657 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 31 0.13457197430109394 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30 0.13023094287202638 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAACTGTCTC 30 0.13023094287202638 No Hit CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 29 0.12588991144295883 No Hit GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA 29 0.12588991144295883 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT 28 0.1215488800138913 No Hit GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 25 0.10852578572668867 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAA 25 0.10852578572668867 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 24 0.10418475429762111 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 24 0.10418475429762111 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.004341031429067547 5 0.0 0.0 0.004341031429067547 6 0.0 0.0 0.004341031429067547 7 0.0 0.0 0.008682062858135093 8 0.0 0.0 0.008682062858135093 9 0.0 0.0 0.017364125716270187 10 0.0 0.0 0.030387220003472826 11 0.0 0.0 0.030387220003472826 12 0.0 0.0 0.03906928286160792 13 0.0 0.0 0.04341031429067546 14 0.0 0.0 0.056433408577878104 15 0.0 0.0 0.06077444000694565 16 0.0 0.0 0.08247959715228338 17 0.0 0.0 0.08682062858135092 18 0.0 0.0 0.10418475429762111 19 0.0 0.0 0.11286681715575621 20 0.0 0.0 0.13457197430109394 21 0.0 0.0 0.14325403715922902 22 0.0 0.0 0.20402847716617467 23 0.0 0.0 0.31255426289286337 24 0.0 0.0 0.3906928286160792 25 0.0 0.0 0.4427852057648897 26 0.0 0.0 0.5599930543497135 27 0.0 0.0 0.6424726515019968 28 0.0 0.0 0.9072755686751172 29 0.0 0.0 1.1503733287028999 30 0.0 0.0 1.358742837298142 31 0.0 0.0 2.0706719916652196 32 0.0 0.0 2.539503386004515 33 0.0 0.0 2.9301962146205938 34 0.0 0.0 3.5205764889737803 35 0.0 0.0 3.972043757596805 36 0.0 0.0 4.922729640562598 37 0.0 0.0 5.704115297794756 38 0.0 0.0 6.728598715054697 39 0.0 0.0 7.857266886612259 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTCGT 290 0.0 43.448273 37 TGCCGTC 290 0.0 43.448273 45 TATGCCG 295 0.0 42.711864 43 ATGCCGT 295 0.0 42.711864 44 TCGTATG 295 0.0 42.711864 40 CTCGTAT 295 0.0 42.711864 39 ACTGCGA 290 0.0 42.672413 27 TGCGACC 290 0.0 42.672413 29 CTGCGAC 290 0.0 42.672413 28 GACTGCG 290 0.0 42.672413 26 GCGACCT 285 0.0 42.631577 30 AGACTGC 285 0.0 42.631577 25 TATCTCG 280 0.0 42.589287 36 ACCTATC 280 0.0 42.589287 33 GACCTAT 280 0.0 42.589287 32 CGACCTA 280 0.0 42.589287 31 AGCCCAC 305 0.0 42.049183 17 GCCCACG 305 0.0 42.049183 18 GAGCCCA 305 0.0 42.049183 16 CGAGCCC 305 0.0 42.049183 15 >>END_MODULE