##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_4.34100000006856.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 72729 Sequences flagged as poor quality 0 Sequence length 51 %GC 25 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.49849441075774 38.0 38.0 38.0 32.0 38.0 2 36.38215842373744 38.0 38.0 38.0 32.0 38.0 3 36.400651734521304 38.0 38.0 38.0 32.0 38.0 4 36.40135296786701 38.0 38.0 38.0 32.0 38.0 5 36.38210342504365 38.0 38.0 38.0 32.0 38.0 6 38.35351785395097 40.0 38.0 40.0 38.0 40.0 7 38.31455127940711 40.0 38.0 40.0 38.0 40.0 8 38.269658595608355 40.0 38.0 40.0 38.0 40.0 9 38.31650373303634 40.0 38.0 40.0 38.0 40.0 10 38.27885712714323 40.0 38.0 40.0 38.0 40.0 11 38.22435342160624 40.0 38.0 40.0 38.0 40.0 12 38.214192412930196 40.0 38.0 40.0 38.0 40.0 13 38.122468341376894 40.0 38.0 40.0 38.0 40.0 14 38.108663669237856 40.0 38.0 40.0 38.0 40.0 15 37.97725804012155 40.0 38.0 40.0 32.0 40.0 16 37.884777736528754 40.0 38.0 40.0 32.0 40.0 17 38.09512024089428 40.0 38.0 40.0 32.0 40.0 18 38.0043448968087 40.0 38.0 40.0 32.0 40.0 19 38.20632759971951 40.0 38.0 40.0 38.0 40.0 20 38.14453656725653 40.0 38.0 40.0 38.0 40.0 21 38.2470678821378 40.0 38.0 40.0 38.0 40.0 22 38.28874314235037 40.0 38.0 40.0 38.0 40.0 23 38.31261257545133 40.0 38.0 40.0 38.0 40.0 24 38.284384495868224 40.0 38.0 40.0 38.0 40.0 25 38.2809745768538 40.0 38.0 40.0 38.0 40.0 26 38.233001966203304 40.0 38.0 40.0 38.0 40.0 27 38.14812523202574 40.0 38.0 40.0 32.0 40.0 28 38.07243327970961 40.0 38.0 40.0 32.0 40.0 29 37.98744654814448 40.0 38.0 40.0 32.0 40.0 30 37.896148716467984 40.0 38.0 40.0 32.0 40.0 31 37.8140906653467 40.0 38.0 40.0 32.0 40.0 32 37.685311223858434 40.0 38.0 40.0 32.0 40.0 33 37.53799722256596 40.0 38.0 40.0 32.0 40.0 34 37.39506936710253 40.0 38.0 40.0 32.0 40.0 35 37.231255757675754 40.0 38.0 40.0 32.0 40.0 36 36.96507582944905 40.0 38.0 40.0 32.0 40.0 37 36.654291960565935 40.0 38.0 40.0 27.0 40.0 38 36.337705729488924 40.0 38.0 40.0 27.0 40.0 39 35.87443798209792 40.0 38.0 40.0 27.0 40.0 40 35.46090280355842 40.0 38.0 40.0 13.0 40.0 41 34.88517647705867 40.0 38.0 40.0 13.0 40.0 42 34.24607790564974 40.0 32.0 40.0 13.0 40.0 43 33.56794401132973 38.0 32.0 40.0 13.0 40.0 44 32.754197087819165 38.0 27.0 40.0 13.0 40.0 45 31.916429484799735 38.0 27.0 40.0 13.0 40.0 46 30.97846801138473 38.0 13.0 40.0 13.0 40.0 47 30.111193609151783 38.0 13.0 40.0 13.0 40.0 48 29.189992987666542 38.0 13.0 40.0 13.0 40.0 49 28.319226168378503 32.0 13.0 40.0 13.0 40.0 50 27.45129178182018 32.0 13.0 40.0 13.0 40.0 51 26.519613909169657 27.0 13.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.3248120129213561 2201 2 0.5260825021376689 2201 3 0.2634836309456219 2201 4 0.46582275343844515 2201 5 0.5440609368945815 2201 6 0.23622828605721935 2201 7 0.8619933073078627 2201 8 1.0617735645162796 2201 9 0.5061157539797634 2201 10 0.9934025214818263 2201 11 0.7033641757837046 2201 12 0.8644780817448279 2201 13 -0.028865928967739762 2201 14 0.118412003355445 2201 15 0.7335755822033434 2201 16 1.0347439985343385 2201 17 0.01117635539840478 2201 18 -0.01234560187558742 2201 19 -0.027771095081206454 2201 20 -0.13204845213940075 2201 21 0.11678684604131462 2201 22 -9.444174514285919E-4 2201 23 -0.18912609297848348 2201 24 0.2702122180502826 2201 25 0.05745361243261726 2201 26 -0.11357715638364141 2201 27 0.4853672591956908 2201 28 0.29320575735415133 2201 29 0.1887216072426554 2201 30 0.8021289733209613 2201 31 0.5775876762327314 2201 32 0.05928895640045084 2201 33 0.3936518639876567 2201 34 -0.1537072071683312 2201 35 0.19552771514993594 2201 36 0.09702255432520701 2201 37 0.20125246650342632 2201 38 0.19673621416769294 2201 39 -0.2782356361640552 2201 40 -0.8086267606262254 2201 41 -1.4295117886561997 2201 42 -0.7723553680887107 2201 43 -0.8042153462711354 2201 44 -0.3966944097323335 2201 45 -1.295539984683412 2201 46 -0.49844796693238536 2201 47 -0.370705455231608 2201 48 0.14473156800808695 2201 49 -1.0060181930328405 2201 50 -0.19269831291298445 2201 51 0.12785716816639692 1101 1 0.5366104092558359 1101 2 0.20935856170239475 1101 3 0.3180082586661257 1101 4 0.5117563158668545 1101 5 0.7440609368945843 1101 6 0.7661251932737159 1101 7 0.5756244642379826 1101 8 0.8136635988805381 1101 9 0.5155086520324517 1101 10 0.5198630025814808 1101 11 0.5525050692544937 1101 12 -0.18546464448655797 1101 13 0.6514318946290487 1101 14 0.3940133779258872 1101 15 0.9874129247004788 1101 16 1.1919032196110848 1101 17 0.34542606902955697 1101 18 0.8019728402779052 1101 19 0.5739471179772124 1101 20 0.32430893617674883 1101 21 0.28608810606421997 1101 22 -0.014231931769870698 1101 23 0.023703231191049667 1101 24 0.4817815193103101 1101 25 0.26570103511303245 1101 26 0.3548077233414446 1101 27 0.3613122763778236 1101 28 0.23089189710214697 1101 29 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-0.2634046814171427 1213 30 -0.89452051121512 1213 31 -0.3675798495404692 1213 32 -0.8176440332902715 1213 33 -0.2944924659092507 1213 34 -0.10390050613740698 1213 35 -0.22335115082944412 1213 36 0.1680277089643809 1213 37 0.09029885825600559 1213 38 0.014713018291402591 1213 39 0.42008910610398686 1213 40 0.5900201465902697 1213 41 0.312240788663388 1213 42 0.728417827787581 1213 43 0.6691738289866009 1213 44 1.4806251778965276 1213 45 0.7809419740794752 1213 46 0.9617324454387486 1213 47 0.9058409365209705 1213 48 0.9914454855338626 1213 49 1.8719457244929352 1213 50 2.4314640582210387 1213 51 1.0580762403313457 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 2.0 17 4.0 18 5.0 19 2.0 20 9.0 21 23.0 22 54.0 23 128.0 24 218.0 25 349.0 26 583.0 27 866.0 28 1218.0 29 1578.0 30 2024.0 31 2586.0 32 3382.0 33 4317.0 34 5284.0 35 6232.0 36 6846.0 37 7464.0 38 8611.0 39 20943.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.4443894253253 27.706252922890705 11.028582432395257 14.820775219388738 2 10.368771312286878 30.355571444285555 31.483060169398307 27.792597074029256 3 34.750924665539195 34.976419310041386 22.209847516121492 8.062808508297929 4 14.676401435465907 21.501739333690825 36.72950267431149 27.092356556531783 5 12.365081329318429 28.23220448514348 38.8758266991159 20.526887486422197 6 37.44998556284288 33.78157268764867 11.072612025464396 17.695829724044053 7 10.42912730822643 38.32858969599472 26.495620729007683 24.746662266771164 8 19.059797329813417 23.084326747239754 31.016513357807753 26.83936256513908 9 19.237168117257212 25.91675947696242 27.841713759298216 27.004358646482146 10 13.46230527024983 21.773982867906888 27.741341143147853 37.02237071869543 11 18.687438125618744 24.231382686173138 18.922560774392256 38.158618413815866 12 21.008126057006145 36.400885478969876 28.017709579397486 14.57327888462649 13 27.56534532304858 17.013845921159373 24.880034099190144 30.540774656601904 14 21.982977904274772 24.233799447263127 34.95304486518445 18.830177783277648 15 34.65880185345598 14.819398039296566 29.51504901758583 21.00675108966162 16 11.826094130264405 31.58712480578586 24.58854102215072 31.998240041799008 17 19.183544390820717 27.836213889920113 34.321934854047214 18.65830686521195 18 10.011550202128538 26.386711767455928 47.750460633061074 15.851277397354455 19 22.2318469936339 35.82477416161366 34.89804617140343 7.045332673349009 20 10.72062038526585 23.190199232768222 41.361767658018124 24.727412723947808 21 14.006792338681956 34.51718021696985 38.398713030565524 13.077314413782673 22 3.3232960717182967 32.83284521992603 40.85302974054366 22.990828967812014 23 3.437418361313919 37.48710968114508 46.472521277619656 12.602950679921351 24 5.640116047243878 41.56938772704149 47.503746786013835 5.286749439700808 25 6.984834110189883 37.91472452529253 45.241925504269275 9.85851586024832 26 6.393598152043889 35.870148083983004 50.510800368491246 7.225453395481857 27 5.318373688624895 39.049072584526115 50.318304940257676 5.314248786591318 28 2.403442918230692 40.5271624798911 49.49332453354233 7.576070068335877 29 4.977381787182555 40.29479299866628 50.98378913500804 3.7440360791431204 30 2.28528999257487 41.47210076176333 51.76690592085361 4.475703324808184 31 7.611819219293542 41.36314262536265 48.11010738494961 2.914930770394203 32 2.8063083501766832 41.2586451071787 48.38097595182114 7.554070590823468 33 5.462745259800079 44.05120378391013 47.63849358577733 2.8475573705124506 34 3.407169079734356 44.73868745617292 46.266276175940824 5.587867288151906 35 2.5615641628511323 48.13898169918465 45.82216172365906 3.4772924143051602 36 2.6784363871358052 48.588596020844506 46.19752780871455 2.5354397833051467 37 2.9176807050832543 49.83294146764015 44.768936737752476 2.4804410895241236 38 3.2009239780555214 48.81271569800217 44.99855628428825 2.9878040396540584 39 3.6959122220847256 48.88558896726203 43.39671932791596 4.021779482737285 40 4.314647527121231 48.54459706581969 43.58783978880501 3.552915618254066 41 4.927882962779634 48.413975168089756 41.78250766544295 4.875634203687662 42 6.642467241403017 47.85023855683427 41.02765059329841 4.479643608464299 43 6.385348347976735 47.42949854940945 40.76640679783855 5.4187463047752615 44 7.431698497160692 47.78973999367515 38.67370649947064 6.1048550096935195 45 8.379050997538808 46.091655323186075 38.24196675328961 7.287326925985507 46 10.260006324849785 45.494919495661975 36.11351730396403 8.131556875524208 47 10.541874630477526 44.841810007012334 34.47868113132313 10.13763423118701 48 11.674847722366593 44.00857979622984 33.43645588417275 10.880116597230815 49 13.609426776114066 43.142350369178736 32.17561082924281 11.072612025464396 50 13.646550894416256 42.252746497270685 32.34473181261945 11.755970795693601 51 16.274337256627433 41.28258717412826 29.574579254207457 12.86849631503685 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 77.0 1 58.0 2 39.0 3 147.5 4 256.0 5 273.0 6 290.0 7 209.5 8 129.0 9 189.5 10 250.0 11 561.5 12 873.0 13 1316.0 14 1759.0 15 2425.0 16 3091.0 17 3690.0 18 4289.0 19 5183.0 20 6077.0 21 6887.0 22 7697.0 23 7532.0 24 7367.0 25 8584.5 26 7859.0 27 5916.0 28 7172.0 29 8428.0 30 9093.0 31 9758.0 32 5972.0 33 2186.0 34 1819.0 35 1452.0 36 1095.5 37 739.0 38 556.5 39 374.0 40 309.5 41 245.0 42 217.5 43 190.0 44 158.5 45 127.0 46 109.5 47 92.0 48 164.5 49 237.0 50 510.0 51 783.0 52 441.0 53 99.0 54 70.0 55 41.0 56 29.5 57 18.0 58 17.0 59 16.0 60 12.0 61 8.0 62 9.0 63 10.0 64 7.0 65 4.0 66 3.5 67 3.0 68 2.0 69 1.0 70 1.5 71 2.0 72 3.0 73 4.0 74 2.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03712411830219032 2 0.0013749673445255675 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0013749673445255675 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.004124902033576703 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.004124902033576703 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0013749673445255675 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 72729.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.986979333076494 #Duplication Level Percentage of deduplicated Percentage of total 1 85.18732789098043 27.248852966888016 2 6.740197284741576 4.311971024957742 3 2.403996208993207 2.3068973116157987 4 1.227498294198539 1.570558502716613 5 0.8054336967977745 1.2881695506816904 6 0.5389273914283536 1.0343195598988542 7 0.3743813692603282 0.8382730384853299 8 0.3682251332431066 0.942272778157327 9 0.2931786111777191 0.8440108358977593 >10 1.6483596063914443 10.231329201374932 >50 0.15897455195334423 3.675026864126437 >100 0.17186438022656472 11.273838942502005 >500 0.05155931406796941 11.585817662476156 >1k 0.030076266539648823 22.848661760221365 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4448 6.115854748449724 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2621 3.603789410001512 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAA 2300 3.162424892408805 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2013 2.7678092645299675 No Hit ACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAA 1904 2.6179378239766806 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAA 1840 2.5299399139270444 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1499 2.061076049443826 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 916 1.25947008758542 No Hit ATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 898 1.2347206753839597 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 887 1.2195960345941785 No Hit GTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 760 1.0449751818394313 No Hit GATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 699 0.9611021738233716 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 648 0.8909788392525677 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 631 0.8676043943956331 RNA PCR Primer, Index 27 (95% over 21bp) AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 621 0.8538547209503774 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 618 0.8497298189168008 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 615 0.845604916883224 No Hit TGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 600 0.8249804067153406 No Hit CGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 537 0.7383574640102298 No Hit GCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT 489 0.6723590314730025 No Hit GTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAA 488 0.6709840641284769 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 415 0.5706114479781106 No Hit AAAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAA 407 0.559611709221906 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA 320 0.4399895502481816 No Hit CTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 290 0.3987405299124146 No Hit CTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAA 278 0.38224092177810776 No Hit GCGTTGATACCACTGCTTCGCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 276 0.3794909870890566 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA 264 0.3629913789547498 No Hit CAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 239 0.32861719534161066 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA 230 0.31624248924088055 No Hit ACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 225 0.3093676525182527 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 213 0.2928680443839459 No Hit AAAAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAA 206 0.2832432729722669 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 205 0.28186830562774134 No Hit TTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 202 0.27774340359416466 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 199 0.2736185015605879 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 199 0.2736185015605879 No Hit AAAAAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAA 197 0.2708685668715368 No Hit CCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 193 0.2653686974934345 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 189 0.25986882811533224 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 186 0.25574392608175556 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG 158 0.21724484043503964 No Hit GCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 156 0.21449490574598856 No Hit TACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 148 0.203495166989784 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAT 147 0.20212019964525843 No Hit CGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 139 0.19112046088905388 No Hit GCGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 138 0.1897454935445283 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGC 135 0.18562059151095162 No Hit CTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 134 0.18424562416642606 No Hit TTGCGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 132 0.1814956894773749 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 125 0.17187091806569593 No Hit AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 124 0.17049595072117035 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 122 0.16774601603211922 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 109 0.14987144055328686 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAGT 109 0.14987144055328686 No Hit AAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAA 106 0.14574653851971017 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATA 106 0.14574653851971017 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAGTAC 104 0.14299660383065904 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 101 0.13887170179708233 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 100 0.13749673445255675 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 99 0.13612176710803117 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 97 0.13337183241898004 No Hit TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT 95 0.1306218977299289 RNA PCR Primer, Index 38 (95% over 21bp) TACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 93 0.12787196304087778 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAGTA 91 0.12512202835182665 No Hit AAAAAAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAA 86 0.1182471916291988 No Hit CCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 83 0.1141222895956221 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAC 83 0.1141222895956221 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAGTACT 76 0.10449751818394314 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 75 0.10312255083941757 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAGTACTC 75 0.10312255083941757 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGCG 73 0.10037261615036643 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.002749934689051135 16 0.0 0.0 0.006874836722627837 17 0.0 0.0 0.008249804067153406 18 0.0 0.0 0.012374706100730107 19 0.0 0.0 0.015124640789781242 20 0.0 0.0 0.015124640789781242 21 0.0 0.0 0.019249542823357946 22 0.0 0.0 0.026124379545985784 23 0.0 0.0 0.03299921626861362 24 0.0013749673445255675 0.0 0.03849908564671589 25 0.0013749673445255675 0.0 0.042623987680292595 26 0.0013749673445255675 0.0 0.053623726436497134 27 0.0013749673445255675 0.0 0.0632484978481761 28 0.0013749673445255675 0.0 0.08937287739416189 29 0.0013749673445255675 0.0 0.10174758349489199 30 0.0013749673445255675 0.0 0.12374706100730108 31 0.0013749673445255675 0.0 0.18287065682190048 32 0.0013749673445255675 0.0 0.23099451388029535 33 0.0013749673445255675 0.0 0.2612437954598578 34 0.0013749673445255675 0.0 0.3272422279970851 35 0.0013749673445255675 0.0 0.39324066053431234 36 0.0013749673445255675 0.0 0.43448968087007933 37 0.0013749673445255675 0.0 0.45786412572701396 38 0.0013749673445255675 0.0 0.5101128848189855 39 0.0013749673445255675 0.0 0.549986937810227 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCCCAC 85 0.0 37.058826 17 GCCCACG 85 0.0 37.058826 18 CCACGAG 85 0.0 37.058826 20 GAGCCCA 85 0.0 37.058826 16 CACGAGA 85 0.0 37.058826 21 CCCACGA 85 0.0 37.058826 19 ACGAGAC 85 0.0 37.058826 22 CGAGCCC 85 0.0 37.058826 15 CCGAGCC 85 0.0 37.058826 14 TCCGAGC 85 0.0 37.058826 13 ATCTCGT 80 0.0 36.5625 37 TGCCGTC 80 0.0 36.5625 45 TCTCGTA 80 0.0 36.5625 38 TATCTCG 80 0.0 36.5625 36 CGAGACG 80 0.0 36.5625 23 CTCGTAT 80 0.0 36.5625 39 GAGACGA 80 0.0 36.5625 24 GCTATCT 75 1.8189894E-12 36.0 34 ATTGCTA 75 1.8189894E-12 36.0 31 CGAATTG 75 1.8189894E-12 36.0 28 >>END_MODULE