FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_4.34100000006856.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_4.34100000006856.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72729
Sequences flagged as poor quality0
Sequence length51
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44486.115854748449724No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26213.603789410001512No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAA23003.162424892408805No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20132.7678092645299675No Hit
ACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAA19042.6179378239766806No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAA18402.5299399139270444No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14992.061076049443826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9161.25947008758542No Hit
ATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8981.2347206753839597No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8871.2195960345941785No Hit
GTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7601.0449751818394313No Hit
GATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6990.9611021738233716No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6480.8909788392525677No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC6310.8676043943956331RNA PCR Primer, Index 27 (95% over 21bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6210.8538547209503774No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6180.8497298189168008No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6150.845604916883224No Hit
TGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6000.8249804067153406No Hit
CGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5370.7383574640102298No Hit
GCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT4890.6723590314730025No Hit
GTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAA4880.6709840641284769No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4150.5706114479781106No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAA4070.559611709221906No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA3200.4399895502481816No Hit
CTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAA2900.3987405299124146No Hit
CTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAA2780.38224092177810776No Hit
GCGTTGATACCACTGCTTCGCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2760.3794909870890566No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA2640.3629913789547498No Hit
CAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2390.32861719534161066No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA2300.31624248924088055No Hit
ACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2250.3093676525182527No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2130.2928680443839459No Hit
AAAAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAA2060.2832432729722669No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.28186830562774134No Hit
TTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2020.27774340359416466No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1990.2736185015605879No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1990.2736185015605879No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAA1970.2708685668715368No Hit
CCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1930.2653686974934345No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1890.25986882811533224No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.25574392608175556No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1580.21724484043503964No Hit
GCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1560.21449490574598856No Hit
TACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1480.203495166989784No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1470.20212019964525843No Hit
CGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.19112046088905388No Hit
GCGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT1380.1897454935445283No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGC1350.18562059151095162No Hit
CTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1340.18424562416642606No Hit
TTGCGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1320.1814956894773749No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1250.17187091806569593No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.17049595072117035No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.16774601603211922No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.14987144055328686No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAGT1090.14987144055328686No Hit
AAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAA1060.14574653851971017No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATA1060.14574653851971017No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAGTAC1040.14299660383065904No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1010.13887170179708233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1000.13749673445255675No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.13612176710803117No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA970.13337183241898004No Hit
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT950.1306218977299289RNA PCR Primer, Index 38 (95% over 21bp)
TACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.12787196304087778No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAGTA910.12512202835182665No Hit
AAAAAAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAA860.1182471916291988No Hit
CCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.1141222895956221No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAC830.1141222895956221No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAGTACT760.10449751818394314No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGG750.10312255083941757No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAGTACTC750.10312255083941757No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGCG730.10037261615036643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC850.037.05882617
GCCCACG850.037.05882618
CCACGAG850.037.05882620
GAGCCCA850.037.05882616
CACGAGA850.037.05882621
CCCACGA850.037.05882619
ACGAGAC850.037.05882622
CGAGCCC850.037.05882615
CCGAGCC850.037.05882614
TCCGAGC850.037.05882613
ATCTCGT800.036.562537
TGCCGTC800.036.562545
TCTCGTA800.036.562538
TATCTCG800.036.562536
CGAGACG800.036.562523
CTCGTAT800.036.562539
GAGACGA800.036.562524
GCTATCT751.8189894E-1236.034
ATTGCTA751.8189894E-1236.031
CGAATTG751.8189894E-1236.028
Produced by FastQC (version 0.11.2)