##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_39.34100000006a8a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 72344 Sequences flagged as poor quality 0 Sequence length 51 %GC 33 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.34573703417008 38.0 38.0 38.0 32.0 38.0 2 36.38293431383391 38.0 38.0 38.0 32.0 38.0 3 36.33452670573924 38.0 38.0 38.0 32.0 38.0 4 36.367051863319695 38.0 38.0 38.0 32.0 38.0 5 36.409792104390135 38.0 38.0 38.0 32.0 38.0 6 38.29343138339047 40.0 38.0 40.0 38.0 40.0 7 38.18647019794316 40.0 38.0 40.0 38.0 40.0 8 38.186138449629546 40.0 38.0 40.0 38.0 40.0 9 38.159197721994914 40.0 38.0 40.0 38.0 40.0 10 38.07656474621254 40.0 38.0 40.0 38.0 40.0 11 38.07116001326993 40.0 38.0 40.0 38.0 40.0 12 38.01335286962291 40.0 38.0 40.0 32.0 40.0 13 37.92817649010284 40.0 38.0 40.0 32.0 40.0 14 37.881856131814665 40.0 38.0 40.0 32.0 40.0 15 37.89898263850492 40.0 38.0 40.0 32.0 40.0 16 37.89404788233993 40.0 38.0 40.0 32.0 40.0 17 37.91610914519518 40.0 38.0 40.0 32.0 40.0 18 37.90556231339157 40.0 38.0 40.0 32.0 40.0 19 37.9843940064138 40.0 38.0 40.0 32.0 40.0 20 37.99242508017251 40.0 38.0 40.0 32.0 40.0 21 38.019739024659955 40.0 38.0 40.0 32.0 40.0 22 38.01875760256552 40.0 38.0 40.0 32.0 40.0 23 37.98458752626341 40.0 38.0 40.0 32.0 40.0 24 37.9583517637952 40.0 38.0 40.0 32.0 40.0 25 37.945897379188324 40.0 38.0 40.0 32.0 40.0 26 37.86800563972133 40.0 38.0 40.0 32.0 40.0 27 37.846193188101296 40.0 38.0 40.0 32.0 40.0 28 37.77836448081389 40.0 38.0 40.0 32.0 40.0 29 37.743835010505364 40.0 38.0 40.0 32.0 40.0 30 37.64269324339268 40.0 38.0 40.0 32.0 40.0 31 37.646176600685614 40.0 38.0 40.0 32.0 40.0 32 37.55533285414133 40.0 38.0 40.0 32.0 40.0 33 37.47592060157027 40.0 38.0 40.0 32.0 40.0 34 37.43909653875926 40.0 38.0 40.0 32.0 40.0 35 37.38521508348999 40.0 38.0 40.0 32.0 40.0 36 37.29040418002875 40.0 38.0 40.0 32.0 40.0 37 37.22894780493199 40.0 38.0 40.0 32.0 40.0 38 37.17426462457149 40.0 38.0 40.0 32.0 40.0 39 37.09432710383722 40.0 38.0 40.0 32.0 40.0 40 37.04716355191861 40.0 38.0 40.0 32.0 40.0 41 37.03960245493752 40.0 38.0 40.0 32.0 40.0 42 36.96038372221608 40.0 38.0 40.0 32.0 40.0 43 36.924499612960304 40.0 38.0 40.0 32.0 40.0 44 36.8255142098861 40.0 38.0 40.0 32.0 40.0 45 36.781129050093995 40.0 38.0 40.0 32.0 40.0 46 36.639237531792546 40.0 38.0 40.0 27.0 40.0 47 36.59403682406281 40.0 38.0 40.0 27.0 40.0 48 36.48267997346014 40.0 38.0 40.0 27.0 40.0 49 36.415722105495966 40.0 38.0 40.0 27.0 40.0 50 36.32382782262523 40.0 38.0 40.0 27.0 40.0 51 35.87783368351211 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.004372220573763741 2201 2 0.5220892105994324 2201 3 0.19420505884013295 2201 4 0.38895970334972674 2201 5 0.4226378869367906 2201 6 0.3296957642427216 2201 7 0.05130951048201382 2201 8 0.33820621751854674 2201 9 0.3743351766658307 2201 10 0.34686508832755436 2201 11 0.2823457217176184 2201 12 0.585588243647905 2201 13 0.26464561490649174 2201 14 0.38469986191110905 2201 15 0.3647299518274494 2201 16 0.649026119066697 2201 17 0.6597777312725412 2201 18 0.4586951297994588 2201 19 0.20236752008800352 2201 20 0.19703625346621578 2201 21 0.2601751436388682 2201 22 0.8009786057343575 2201 23 0.04327609287005174 2201 24 0.13936896761319417 2201 25 0.38060154973051397 2201 26 0.1581381756400546 2201 27 0.290227030922928 2201 28 -0.34703810366630705 2201 29 -0.4576452774574591 2201 30 0.7018119676616124 2201 31 0.018504989189693788 2201 32 1.032392648957 2201 33 0.10069620909854393 2201 34 -0.3390398130337502 2201 35 0.26412000597706964 2201 36 0.2630294009649319 2201 37 0.9790830713531946 2201 38 0.3824487458222663 2201 39 0.2831061408827935 2201 40 1.0534708141136662 2201 41 0.7941351333589353 2201 42 0.8137835198221168 2201 43 0.025093564473792185 2201 44 0.9863915363140379 2201 45 0.5200895524866027 2201 46 0.8820597982841178 2201 47 0.024947430978507157 2201 48 0.690433781437001 2201 49 0.34309666121054505 2201 50 1.126362984657689 2201 51 1.087638291772933 1101 1 0.07514636958312337 1101 2 -0.39280440642184544 1101 3 -0.1362269205280171 1101 4 -0.4498539652511795 1101 5 0.4747548007558322 1101 6 0.343127958525983 1101 7 0.31855644794171667 1101 8 0.3405702837124025 1101 9 -0.37346194347171036 1101 10 0.08348662282142527 1101 11 -0.9810327437885107 1101 12 -0.12717771379890053 1101 13 -0.2607143679002988 1101 14 0.12196614173058151 1101 15 -0.4573203383079516 1101 16 -0.30713119879274586 1101 17 2.0326318743713045E-4 1101 18 0.5860323316047484 1101 19 0.15916958327304087 1101 20 -0.6813217951045942 1101 21 0.12123252597284306 1101 22 0.05253674027121491 1101 23 -0.13220209324643406 1101 24 -0.08027427330664239 1101 25 -0.34312507825143257 1101 26 -0.9083780504506365 1101 27 -0.42866400711704244 1101 28 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5 -0.4314121317268871 1103 6 -0.8780309994750795 1103 7 -0.3975520050088406 1103 8 -1.0132308858782437 1103 9 -0.43380218802248294 1103 10 -0.05925659886916179 1103 11 0.26218894680160076 1103 12 0.258787198481798 1103 13 0.5712898851565882 1103 14 0.3229230795296232 1103 15 -0.0697605296955075 1103 16 -0.04955544121698807 1103 17 0.5177471228365604 1103 18 -0.011256344631298987 1103 19 0.35055714308165165 1103 20 0.3476521549219882 1103 21 -0.23123956334134732 1103 22 0.08709282745888203 1103 23 -0.5311919922363302 1103 24 -0.08851457102062454 1103 25 -0.6509400702769526 1103 26 -0.19572520621884593 1103 27 -0.11925411875978398 1103 28 -0.17719861131841697 1103 29 0.23141780578255577 1103 30 -0.5546270021032242 1103 31 0.08961361181007987 1103 32 -0.6641359064741295 1103 33 -0.0030738543579644784 1103 34 -0.1044373494279256 1103 35 0.0024552056784514775 1103 36 -0.1027787363997561 1103 37 -0.1715328301025778 1103 38 -0.5259125830317828 1103 39 0.1996794891470728 1103 40 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2254.0 34 2717.0 35 3237.0 36 4365.0 37 6220.0 38 11068.0 39 34229.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.70877814038387 28.54001880634991 15.982631782731346 20.768571270534874 2 10.161455309502088 28.42746951978104 42.97088828066683 18.44018689005004 3 18.34706402742453 33.578458476169416 31.7787238748203 16.295753621585757 4 12.38388809023554 24.691750525268162 37.58431936304324 25.34004202145306 5 10.361605661837887 39.39234767223267 37.24704191087029 12.999004755059163 6 26.796970032069005 43.413413690147074 16.80858122304545 12.981035054738472 7 17.116830697777285 37.50414685392016 25.662114342585422 19.716908105717128 8 19.042353201371228 42.69324339267942 23.489162888421983 14.775240517527369 9 21.165542408492758 21.119927015371005 24.122249253566295 33.592281322569946 10 13.213258874267389 32.051033948910764 34.771370120535224 19.96433705628663 11 25.813140179423026 25.35697994277262 27.778776108262033 21.05110376954232 12 20.099800951011833 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35.26208116775406 34.14104832467101 15.936359615171956 26 13.026650447860225 35.71408824505142 36.98579011390025 14.2734711931881 27 14.899646135132146 36.02233771978326 35.21508348999226 13.862932655092337 28 11.821298241733938 33.2577684396771 35.096207010947694 19.824726307641267 29 14.566515536879354 33.20800619263519 36.99270153710052 15.23277673338494 30 14.848145536985582 32.510817124925694 36.10224083827534 16.53879649981338 31 16.988278226252348 32.67859117549486 33.35038151056066 16.982749087692138 32 15.92944819197169 34.52808802388588 34.71746101957315 14.82500276456928 33 12.909156253455711 38.18423089682628 35.97810461130156 12.928508238416455 34 13.041855578900808 35.84816985513657 35.751409930332855 15.358564635629769 35 12.56220280880239 36.298794647793876 34.540528585646356 16.59847395775738 36 11.931881012938184 35.609034612407385 38.206347451067124 14.252736923587307 37 12.439179475837664 36.27529580891297 34.00558442994581 17.27994028530355 38 12.600906778723875 36.83235651885436 35.364370231117995 15.20236647130377 39 14.21403295366582 33.02001548158797 35.67676655976999 17.089185004976223 40 14.755888532566628 32.986840650226696 37.088079177264184 15.169191639942497 41 14.762799955766893 31.756607320579455 35.92834236425965 17.552250359394005 42 14.801503925688378 34.52532345460577 35.75555678425301 14.917615835452835 43 11.59322127612518 34.54882229348667 38.04876700210107 15.809189428287073 44 11.052747981864426 35.885491540418 36.344410040915626 16.717350436801944 45 11.97473183677983 33.58398761472963 36.81300453389362 17.628276014596924 46 13.788289284529471 31.434534999447084 35.8827269711379 18.894448744885548 47 14.24582550038704 31.271425411920823 34.756164989494636 19.7265840981975 48 10.733440230012164 31.991595709388477 34.66631648789119 22.608647572708172 49 14.396494526152825 31.398595598805706 35.252405175273694 18.952504699767776 50 10.987780603781932 33.59504589185005 35.65050315160898 19.766670352759043 51 10.403505619220082 32.45738930896726 34.837782170553346 22.30132290125931 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1033.0 1 593.0 2 153.0 3 268.0 4 383.0 5 371.0 6 359.0 7 423.0 8 487.0 9 399.5 10 312.0 11 526.0 12 740.0 13 1371.0 14 2002.0 15 2173.5 16 2345.0 17 1696.5 18 1048.0 19 919.0 20 790.0 21 1192.5 22 1595.0 23 1116.5 24 638.0 25 685.0 26 1324.0 27 1916.0 28 2073.0 29 2230.0 30 3631.5 31 5033.0 32 7393.0 33 9753.0 34 11205.0 35 12657.0 36 11582.5 37 10508.0 38 8636.0 39 6764.0 40 5167.0 41 3570.0 42 2616.5 43 1663.0 44 1322.5 45 982.0 46 778.5 47 575.0 48 498.5 49 422.0 50 462.5 51 503.0 52 574.0 53 645.0 54 1218.5 55 1792.0 56 1005.0 57 218.0 58 187.0 59 156.0 60 137.5 61 119.0 62 99.5 63 80.0 64 67.5 65 55.0 66 45.0 67 35.0 68 30.0 69 25.0 70 19.5 71 14.0 72 10.0 73 6.0 74 4.5 75 2.5 76 2.0 77 1.5 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03870396992148623 2 0.0027645692801061598 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0013822846400530799 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0013822846400530799 17 0.0013822846400530799 18 0.0013822846400530799 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.006911423200265399 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0041468539201592386 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 72344.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.486729989528 #Duplication Level Percentage of deduplicated Percentage of total 1 82.77357618080083 49.23929376534925 2 10.776127589301264 12.82073184474935 3 3.0027529184086874 5.358718562479343 4 1.2054082734844653 2.86823185967654 5 0.5844567985751792 1.7383711883692816 6 0.31131238530723776 1.1111373484300533 7 0.2575846784228866 1.0726009150347158 8 0.18262827364038953 0.8691167041999588 9 0.11516092822165241 0.6165492338218367 >10 0.6456873977975047 7.423089258751141 >50 0.08072736283769674 3.3011299849958715 >100 0.053045568033242375 6.80629221207646 >500 0.006918987101382773 2.964804965732088 >1k 0.004612658067588515 3.809932156334108 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 1461 2.0195178591175496 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1316 1.819086586309853 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 968 1.3380515315713812 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 687 0.9496295477164659 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 506 0.6994360278668583 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 496 0.6856131814663275 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 464 0.641380072984629 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 376 0.519739024659958 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 312 0.43127280769656084 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 307 0.4243613844962955 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 279 0.3856574145748093 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 255 0.3524825832135353 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA 203 0.2806037819307752 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 202 0.2792214972907221 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 201 0.277839212650669 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 189 0.2612517969700321 No Hit TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT 182 0.2515758044896605 Illumina Paired End PCR Primer 2 (95% over 21bp) ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 179 0.24742895056950126 No Hit GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 170 0.23498838880902356 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 150 0.20734269600796196 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA 145 0.20043127280769657 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC 137 0.18937299568727192 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 135 0.18660842640716577 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT 125 0.17278558000663496 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC 122 0.16863872608647573 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 120 0.16587415680636958 No Hit GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 108 0.14928674112573262 No Hit TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 104 0.14375760256552028 No Hit GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 98 0.13546389472520182 No Hit GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 97 0.13408161008514874 No Hit GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCAT 93 0.12855247152493643 No Hit GATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 92 0.12717018688488335 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAA 91 0.12578790224483025 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 91 0.12578790224483025 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC 88 0.12164104832467103 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 85 0.11749419440451178 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT 82 0.11334734048435254 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 78 0.10781820192414021 No Hit GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA 77 0.10643591728408713 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAA 77 0.10643591728408713 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.004146853920159239 11 0.0 0.0 0.004146853920159239 12 0.0 0.0 0.004146853920159239 13 0.0 0.0 0.004146853920159239 14 0.0 0.0 0.004146853920159239 15 0.0 0.0 0.005529138560212319 16 0.0 0.0 0.005529138560212319 17 0.0 0.0 0.005529138560212319 18 0.0 0.0 0.008293707840318479 19 0.0 0.0 0.012440561760477718 20 0.0 0.0 0.012440561760477718 21 0.0 0.0 0.026263408161008514 22 0.0 0.0 0.05943823952228243 23 0.0 0.0 0.13269932544509566 24 0.0 0.0 0.21563640384828045 25 0.0 0.0 0.24742895056950126 26 0.0 0.0 0.3828928452947031 27 0.0 0.0 0.4713590622581002 28 0.0 0.0 0.7008183125069114 29 0.0 0.0 1.0657414574809245 30 0.0 0.0 1.4251354638947251 31 0.0 0.0 2.464613513214641 32 0.0 0.0 3.240075196284419 33 0.0 0.0 3.956098639831914 34 0.0 0.0 4.958255003870397 35 0.0 0.0 5.8360057503041025 36 0.0 0.0 7.153323012274687 37 0.0 0.0 8.371115780161452 38 0.0 0.0 9.732666150613735 39 0.0 0.0 11.237974123631538 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 170 0.0 42.34708 45 ATCTCGT 190 0.0 37.889496 37 ATGCCGT 190 0.0 37.889496 44 TCTCGTA 185 0.0 37.697487 38 TATCTCG 185 0.0 37.697487 36 CGACCTA 175 0.0 37.280556 31 CTATCTC 175 0.0 37.280556 35 TATGCCG 195 0.0 36.91797 43 CGTATGC 195 0.0 36.91797 41 TCGTATG 195 0.0 36.91797 40 CTCGTAT 190 0.0 36.705452 39 ACTGCGA 180 0.0 36.27007 27 GCGACCT 180 0.0 36.27007 30 TGCGACC 180 0.0 36.27007 29 CTGCGAC 180 0.0 36.27007 28 CCTATCT 180 0.0 36.244987 34 ACCTATC 180 0.0 36.244987 33 CCACGAG 205 0.0 36.2145 20 CCCACGA 205 0.0 36.2145 19 GTGTAGG 25 0.0020813192 36.044872 1 >>END_MODULE