FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_38.34100000006a7d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_38.34100000006a7d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104296
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC5440.5215923908874741No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA2270.21764976605047173No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT1750.16779166986269847No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA1230.11793357367492523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT950.037.89473739
TCTCGTA950.037.89473738
ACGAGAC950.037.89473722
CCACGAG950.037.89473720
TGCCGTC950.037.89473745
TATCTCG900.037.536
CGAGACT800.036.562523
GCGACCT751.8189894E-1236.00000430
GACTGCG751.8189894E-1236.00000426
TCTGAGC250.002098350336.045
TCCGAGC1000.036.013
TATGCCG1000.036.043
CCGAGCC1000.036.014
TCGTATG1000.036.040
GCCCACG1050.034.28571318
CGTATGC1050.034.28571341
CCCACGA1050.034.28571319
GTATGCC1050.034.28571342
CGAGCCC1050.034.28571315
ATGCCGT1050.034.28571344
Produced by FastQC (version 0.11.2)