FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_37.34100000006a60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_37.34100000006a60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103661
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC7010.676242752819286No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4450.4292839158410588No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.19004254251840133No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT1790.17267824929336975No Hit
GTGTAGATCAATGAGTTACAATGAGAAACATGGAAAATGACAAAAACCACA1480.14277307762803754No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA1440.13891434580025275No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA1360.13119688214468314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.12540878440300596No Hit
GATCTACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTGT1300.12540878440300596No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC1180.11383258891965156No Hit
TTGTAGAACAGTGTAGATCAATGAGTTACAATGAGAAACATGGAAAATGAC1060.10225639343629715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG750.041.9986518
CGTATGC750.041.9986541
CTCGTAT750.041.9986539
GTATGCC750.041.9986542
TCGTATG750.041.9986540
ACGAGAC750.041.9986522
CCACGAG750.041.9986520
CTGCGAC700.041.7843728
TGCCGTC800.039.3927445
CACGAGA800.039.37373421
TATGCCG800.039.37373443
CCCACGA800.039.37373419
TATCTCG800.039.37373436
TCTCGTA800.039.37373438
CGAGACT800.039.37373423
ATGCCGT800.039.37373444
ACTGCGA750.038.9987527
GCGACCT750.038.9987530
CGACCTA750.038.9987531
TGCGACC750.038.9987529