##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_36.34100000006a54.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 70867 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.31240210535228 38.0 38.0 38.0 32.0 38.0 2 36.3781026429791 38.0 38.0 38.0 32.0 38.0 3 36.32785358488436 38.0 38.0 38.0 32.0 38.0 4 36.400270930052066 38.0 38.0 38.0 32.0 38.0 5 36.422636770288 38.0 38.0 38.0 32.0 38.0 6 38.3314377636982 40.0 38.0 40.0 38.0 40.0 7 38.22063866115399 40.0 38.0 40.0 38.0 40.0 8 38.19636784398944 40.0 38.0 40.0 38.0 40.0 9 38.20194166537316 40.0 38.0 40.0 38.0 40.0 10 38.18955225986708 40.0 38.0 40.0 38.0 40.0 11 38.176866524616536 40.0 38.0 40.0 38.0 40.0 12 38.06984915404914 40.0 38.0 40.0 38.0 40.0 13 38.138809318864915 40.0 38.0 40.0 38.0 40.0 14 38.08321221442985 40.0 38.0 40.0 38.0 40.0 15 38.07007492909253 40.0 38.0 40.0 38.0 40.0 16 38.07178235285817 40.0 38.0 40.0 38.0 40.0 17 38.07646718500854 40.0 38.0 40.0 38.0 40.0 18 38.08370609733726 40.0 38.0 40.0 38.0 40.0 19 38.100328784906935 40.0 38.0 40.0 38.0 40.0 20 38.1060719375732 40.0 38.0 40.0 38.0 40.0 21 38.098085145413236 40.0 38.0 40.0 38.0 40.0 22 38.10562038748642 40.0 38.0 40.0 38.0 40.0 23 38.07975503407792 40.0 38.0 40.0 38.0 40.0 24 38.078795490143506 40.0 38.0 40.0 38.0 40.0 25 38.07052647917931 40.0 38.0 40.0 38.0 40.0 26 38.01871110672104 40.0 38.0 40.0 32.0 40.0 27 38.014252049614065 40.0 38.0 40.0 32.0 40.0 28 37.966105521610906 40.0 38.0 40.0 32.0 40.0 29 37.97666050488944 40.0 38.0 40.0 32.0 40.0 30 37.97503774676507 40.0 38.0 40.0 32.0 40.0 31 37.92691944064233 40.0 38.0 40.0 32.0 40.0 32 37.90775678383451 40.0 38.0 40.0 32.0 40.0 33 37.87370708510308 40.0 38.0 40.0 32.0 40.0 34 37.87765814836243 40.0 38.0 40.0 32.0 40.0 35 37.89662325200728 40.0 38.0 40.0 32.0 40.0 36 37.850932027601 40.0 38.0 40.0 32.0 40.0 37 37.854092878208476 40.0 38.0 40.0 32.0 40.0 38 37.83765363286156 40.0 38.0 40.0 32.0 40.0 39 37.798636883175526 40.0 38.0 40.0 32.0 40.0 40 37.771035884120955 40.0 38.0 40.0 32.0 40.0 41 37.80771021773181 40.0 38.0 40.0 32.0 40.0 42 37.79692945940988 40.0 38.0 40.0 32.0 40.0 43 37.80113451959304 40.0 38.0 40.0 32.0 40.0 44 37.737875174622886 40.0 38.0 40.0 32.0 40.0 45 37.73709907291123 40.0 38.0 40.0 32.0 40.0 46 37.655834168230626 40.0 38.0 40.0 32.0 40.0 47 37.65026034684691 40.0 38.0 40.0 32.0 40.0 48 37.60820974501531 40.0 38.0 40.0 32.0 40.0 49 37.59848730720928 40.0 38.0 40.0 32.0 40.0 50 37.585787461018526 40.0 38.0 40.0 32.0 40.0 51 37.13832954689771 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.5351741024126966 2201 2 0.321169362593281 2201 3 0.36727891032355586 2201 4 0.38029184335594124 2201 5 0.7218728588766297 2201 6 0.12650317039576464 2201 7 0.46406190745815934 2201 8 0.4560506748181581 2201 9 0.9355307348077204 2201 10 0.8718483337135154 2201 11 0.5823893124160691 2201 12 0.2561457087920189 2201 13 0.44561960862284167 2201 14 0.4014965956976866 2201 15 0.29414879144647443 2201 16 0.055840576854471635 2201 17 0.12196821456235796 2201 18 0.8504232234487574 2201 19 0.7327713490287096 2201 20 0.7142874508522894 2201 21 0.4947488354308902 2201 22 0.8189493632509297 2201 23 0.6886170096433659 2201 24 0.19693467147062904 2201 25 0.7453582379694623 2201 26 0.6742137538053612 2201 27 0.8169013071198492 2201 28 0.4902473483189027 2201 29 0.559584149539333 2201 30 0.3313005274163956 2201 31 1.1032565104202305 2201 32 1.2819944188280914 2201 33 0.3540762302064806 2201 34 0.6261759944787144 2201 35 0.639115051241852 2201 36 0.8119114730865817 2201 37 0.3963143734414203 2201 38 0.5219242108876543 2201 39 0.7921467395311197 2201 40 0.30854632351297795 2201 41 0.6772095431217693 2201 42 0.730553060096625 2201 43 0.21347644496595564 2201 44 0.4290244412554429 2201 45 0.11252497386965388 2201 46 0.3164675296709589 2201 47 0.08120715770576936 2201 48 0.02191449962428038 2201 49 0.004623185619635706 2201 50 0.48229236330956127 2201 51 0.4958833394061344 1101 1 -0.5011625312506709 1101 2 0.0966149071477389 1101 3 0.08351653408593762 1101 4 0.3926680809797034 1101 5 0.007912462837026624 1101 6 -0.04646712663393515 1101 7 0.29911141240865646 1101 8 -0.13503843409273486 1101 9 -0.2245682750932687 1101 10 0.09838298717886573 1101 11 0.5943695104358753 1101 12 0.28218531275241787 1101 13 -0.5305190052385456 1101 14 -0.16731528549043162 1101 15 -0.42218784221689276 1101 16 -0.6286148686900859 1101 17 -0.256348617120814 1101 18 -0.2976955884324326 1101 19 -0.08742667077327582 1101 20 -0.6598709649892953 1101 21 -0.5403996794205952 1101 22 -0.6750110327886745 1101 23 0.2060427522176198 1101 24 -0.6969267146679883 1101 25 -0.044938791733507344 1101 26 0.15233256568654951 1101 27 -0.6957719602068835 1101 28 0.356881011685239 1101 29 0.30770296142052445 1101 30 0.32823122048569786 1101 31 -0.5107038856193711 1101 32 -0.41305508612240516 1101 33 0.16358118070152727 1101 34 -0.3588735104717813 1101 35 -0.29593445370863947 1101 36 -0.17462318037876656 1101 37 -0.5074480027962025 1101 38 -0.7591648980232364 1101 39 -0.887061181260961 1101 40 -1.0485823893583088 1101 41 -0.010513229155456827 1101 42 -0.5418231775271352 1101 43 -0.0236522679053337 1101 44 -0.08770823201188449 1101 45 -0.7866829469224257 1101 46 0.03953683660165552 1101 47 -0.7376047234823488 1101 48 -0.5779864904747356 1101 49 -0.8925055272516502 1101 50 -0.9236482307498477 1101 51 -0.7874829972275279 2202 1 -0.008612305354297689 2202 2 -0.25203452090186573 2202 3 -0.14709002171527885 2202 4 0.24194232879283106 2202 5 0.009931111303814077 2202 6 0.044852684958875955 2202 7 -0.13768566535737392 2202 8 0.5812933932647582 2202 9 0.07621034645820401 2202 10 0.6644696929368195 2202 11 0.43316601144519495 2202 12 0.4977961942289113 2202 13 0.5061050455160441 2202 14 0.8058655277365219 2202 15 0.83735267494162 2202 16 0.7025396059806823 2202 17 0.48478374854293804 2202 18 0.696248466167205 2202 19 0.6015092131063753 2202 20 0.4194330819202534 2202 21 0.49814689368331955 2202 22 0.5211823729596645 2202 23 0.35638399993462855 2202 24 1.0431482637036353 2202 25 0.36225144185295477 2202 26 0.5758642392422502 2202 27 0.1631148993528555 2202 28 0.5056842415227862 2202 29 0.5370598776946736 2202 30 0.7300383914940625 2202 31 0.8117031123619824 2202 32 0.09170315669216933 2202 33 0.5606781719540521 2202 34 0.2818070624398743 2202 35 0.7622218473583615 2202 36 0.5893872012419195 2202 37 0.5629163151889927 2202 38 0.5105649875866831 2202 39 0.6096224676864566 2202 40 0.7628181681731689 2202 41 0.5861415819567171 2202 42 0.33705791446556077 2202 43 0.9625055711795483 2202 44 0.2972768684399085 2202 45 0.6905832262968374 2202 46 0.4047199568554234 2202 47 -0.2013171141388952 2202 48 0.7328853734106815 2202 49 0.40433192348371705 2202 50 0.8415156642804362 2202 51 0.3321940190177841 1102 1 0.15929174947152092 1102 2 -0.18236004917142168 1102 3 0.10923969463728156 1102 4 0.5224487061010379 1102 5 0.19030423142564956 1102 6 0.4702286605918431 1102 7 -0.17633024940457886 1102 8 0.7168349885436456 1102 9 0.14788367598418972 1102 10 0.4185150003801823 1102 11 0.0276834300631279 1102 12 0.22928296369398282 1102 13 0.47326666744636725 1102 14 0.1769867917761161 1102 15 0.19905075223078939 1102 16 0.5630954788152565 1102 17 0.6766740969152991 1102 18 0.1355212626644402 1102 19 0.42218311373459017 1102 20 0.10075803908758729 1102 21 -0.18270214496126158 1102 22 0.22306701030975518 1102 23 0.26234249983944125 1102 24 0.30909153421572455 1102 25 0.5822209830675007 1102 26 0.7485274792955536 1102 27 0.5565091502571065 1102 28 0.23593362282871055 1102 29 0.07056454169619997 1102 30 0.619339743102671 1102 31 0.1324721966947422 1102 32 0.8270924580437722 1102 33 0.42780172040255593 1102 34 0.2930387395767511 1102 35 0.9834287767320475 1102 36 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1213 29 -0.36454283458764536 1213 30 -0.7167947106788475 1213 31 -0.7638863467226216 1213 32 -1.2513389145052471 1213 33 -0.4335428174125724 1213 34 -0.7765224182196988 1213 35 -1.0389801868533866 1213 36 -0.836025034849925 1213 37 -0.9551141979871502 1213 38 -0.701409122445682 1213 39 -0.45864691126253376 1213 40 -1.0074854225187693 1213 41 -1.203425377513149 1213 42 -0.6048437653001955 1213 43 -0.7603330788435727 1213 44 -0.9117692095382068 1213 45 -0.5408083594636821 1213 46 -0.16146897826554607 1213 47 -0.0076817311831192114 1213 48 -0.35379978609000773 1213 49 -0.4545831635867117 1213 50 -0.684374303357103 1213 51 -0.6637992002764008 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 2.0 17 2.0 18 0.0 19 0.0 20 0.0 21 5.0 22 17.0 23 42.0 24 71.0 25 188.0 26 279.0 27 378.0 28 594.0 29 772.0 30 1003.0 31 1209.0 32 1479.0 33 1845.0 34 2186.0 35 2847.0 36 3909.0 37 5932.0 38 11400.0 39 36707.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.24428256818567 23.321474956236944 14.988141622903608 25.44610085267378 2 12.798622772235312 23.250596187223955 43.67618214401626 20.274598896524477 3 17.67113042742038 27.05349457434349 31.99937911863067 23.275995879605457 4 12.845188874934738 20.295765306842394 38.91091763444198 27.948128183780884 5 12.636346959797931 38.26604766675604 37.41656906599687 11.681036307449165 6 28.183780885320388 41.03038085427632 18.279311950555265 12.506526309848025 7 24.299039044971565 34.073687329786786 23.637235949031286 17.990037676210367 8 21.131132967389618 42.125389814723356 21.822569037774986 14.920908180112042 9 23.39311668336461 19.117501799144875 23.222374306800063 34.26700721069045 10 14.497579973753652 31.955635203973642 34.52805960461146 19.018725217661252 11 30.670994143794537 24.65674169194948 28.06321879630283 16.60904536795315 12 21.07045592447825 26.551145102798202 32.51160624832433 19.866792724399225 13 25.238827663087193 24.838076961067916 29.198357486559328 20.724737889285564 14 17.737451846416526 25.954252331832873 28.626864407975503 27.681431413775098 15 20.089745579747976 35.3323831966924 23.752945658769246 20.82492556479038 16 20.178372352285397 32.01388594894374 29.17037099755867 18.6373707012122 17 19.016171365676065 32.921288064798354 29.48804786498462 18.574492704540965 18 20.21788521513343 29.957805907172997 31.82478867674245 17.99952020095113 19 20.555406606742206 28.846995075281868 29.54971989783679 21.047878420139135 20 20.314109529117925 30.472575387698082 29.58499724836666 19.62831783481733 21 19.268488859412702 29.64567429127803 28.965526973062218 22.120309876247056 22 20.0050799384763 32.73597019769427 28.95000493882907 18.308944925000354 23 18.977803491046608 30.849337491357048 29.75573962493121 20.417119392665136 24 21.437340369988853 29.56947521413352 30.35263239589654 18.64055201998109 25 19.223333850734477 31.341809304753976 30.617918071881128 18.816938772630422 26 19.96020658707984 30.705839189456157 30.45324979538848 18.880704428075525 27 19.326343714281684 31.68752733994666 29.525731299476483 19.46039764629517 28 18.888904567711347 30.12121297642062 29.459409880480337 21.530472575387698 29 20.280243272609255 29.66966288963834 30.472575387698082 19.577518450054328 30 20.404448082187916 29.47842285004657 29.791707826479634 20.32542124128588 31 20.779770556112155 29.006448699676863 29.003626511634472 21.210154232576517 32 20.513073786106368 30.179067831289597 29.6273300690025 19.680528313601535 33 18.74779516559188 31.499851835127775 30.407665062723126 19.34468793655721 34 19.398309509362612 29.55113099185799 30.58546290939365 20.46509658938575 35 19.116090705123682 30.060535933509247 30.222811745946633 20.600561615420435 36 19.024369593746034 29.275967657725033 32.33663058969619 19.363032158832745 37 19.30658839798496 30.368154430129678 29.130624973541984 21.194632198343374 38 19.423709201744114 29.816416667842578 30.66730636262294 20.092567767790367 39 20.455218931237386 28.50692141617396 29.267501093597865 21.770358558990786 40 20.69087163277689 27.86769582457279 31.911891289316607 19.52954125333371 41 20.369142195944516 27.77315252515275 30.62074025992352 21.236965018979216 42 20.858791821299054 28.7072967671836 30.81264904680599 19.621262364711363 43 18.361155403784554 29.100991999096898 32.28442011091199 20.253432486206556 44 18.235568035898233 29.98151466832235 30.84651530331466 20.93640199246476 45 18.623618891726757 28.69883020305643 31.52807371555167 21.14947718966515 46 20.26613233239731 27.917084115314605 30.248211438328138 21.568572113959952 47 19.744027544555294 27.74916392679244 29.99280342049191 22.514005108160358 48 18.21157943753792 28.01727179081942 30.387909746426406 23.383239025216252 49 20.23932154599461 28.037027107116145 30.387909746426406 21.33574160046284 50 17.89972765885391 29.380388615293434 31.331931646605614 21.387952079247043 51 17.688313154404085 28.54683487144752 30.64516129032258 23.119690683825812 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 159.0 1 83.0 2 7.0 3 13.0 4 19.0 5 14.0 6 9.0 7 11.0 8 13.0 9 20.5 10 28.0 11 37.0 12 46.0 13 79.5 14 113.0 15 154.0 16 195.0 17 166.5 18 138.0 19 120.5 20 103.0 21 112.0 22 121.0 23 119.5 24 118.0 25 185.5 26 542.5 27 832.0 28 1759.0 29 2686.0 30 4270.0 31 5854.0 32 7073.0 33 8292.0 34 8748.0 35 9204.0 36 8576.5 37 7949.0 38 6800.5 39 5652.0 40 4951.0 41 4250.0 42 3820.0 43 3390.0 44 3181.5 45 2973.0 46 2822.5 47 2672.0 48 2635.0 49 2598.0 50 2495.5 51 2393.0 52 2332.5 53 2272.0 54 2644.0 55 3016.0 56 2265.0 57 1514.0 58 1375.0 59 1236.0 60 1096.0 61 956.0 62 811.5 63 667.0 64 562.0 65 457.0 66 365.5 67 274.0 68 243.0 69 212.0 70 153.5 71 95.0 72 73.0 73 51.0 74 41.5 75 22.0 76 12.0 77 7.0 78 2.0 79 2.5 80 3.0 81 2.0 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043743914657033596 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0028221880423892646 12 0.0 13 0.0 14 0.0 15 0.0 16 0.005644376084778529 17 0.0014110940211946323 18 0.005644376084778529 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0014110940211946323 27 0.0 28 0.0 29 0.0 30 0.00705547010597316 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0014110940211946323 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 70867.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.46343862340957 #Duplication Level Percentage of deduplicated Percentage of total 1 89.65567550762877 73.9331529446367 2 7.162433662187788 11.812778173921307 3 1.7336236661153348 4.288817063603767 4 0.5807425417043922 1.9156010777537207 5 0.3039938803087424 1.2534190345366047 6 0.16910437669263753 0.836695703300595 7 0.08090842633211516 0.46703909342685235 8 0.0747033479312796 0.4928235957675429 9 0.06498041633916106 0.4822657717057207 >10 0.16018488366359812 2.1665973949271584 >50 0.008531470876243383 0.5416959132112985 >100 0.003411881130351793 0.35872814207390136 >500 0.0 0.0 >1k 0.001705939089567666 1.4503860911348145 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 1031 1.4548379358516657 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 151 0.21307519720038948 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 104 0.14675377820424174 No Hit TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT 92 0.12982064994990616 Illumina Paired End PCR Primer 2 (95% over 21bp) GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT 90 0.1269984619075169 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA 80 0.11288752169557056 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.001411094021194632 4 0.0 0.0 0.001411094021194632 5 0.0 0.0 0.001411094021194632 6 0.0 0.0 0.001411094021194632 7 0.0 0.0 0.001411094021194632 8 0.0 0.0 0.001411094021194632 9 0.0 0.0 0.001411094021194632 10 0.0 0.0 0.001411094021194632 11 0.0 0.0 0.001411094021194632 12 0.0 0.0 0.001411094021194632 13 0.0 0.0 0.001411094021194632 14 0.0 0.0 0.001411094021194632 15 0.0 0.0 0.0042332820635838965 16 0.0 0.0 0.0042332820635838965 17 0.0 0.0 0.0042332820635838965 18 0.0 0.0 0.007055470105973161 19 0.0 0.0 0.007055470105973161 20 0.0 0.0 0.008466564127167793 21 0.0 0.0 0.008466564127167793 22 0.0 0.0 0.009877658148362426 23 0.0 0.0 0.011288752169557057 24 0.0 0.0 0.01975531629672485 25 0.0 0.0 0.021166410317919482 26 0.0 0.0 0.03104406846628191 27 0.0 0.0 0.03809953857225507 28 0.0 0.0 0.047977196720617496 29 0.0 0.0 0.07619907714451014 30 0.0 0.0 0.11147642767437595 31 0.0 0.0 0.26528567598459085 32 0.0 0.0 0.5037605655664837 33 0.0 0.0 0.7577574893815174 34 0.0 0.0 1.0696092680655311 35 0.0 0.0 1.3955719869614913 36 0.0 0.0 1.8400666036378004 37 0.0 0.0 2.363582485501009 38 0.0 0.0 2.9943415129750095 39 0.0 0.0 3.7196438398690503 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTAGA 20 6.9647515E-4 45.031075 1 GCGACCT 180 0.0 41.249355 30 CGACCTA 180 0.0 41.249355 31 TGCGACC 180 0.0 41.249355 29 GACCTAT 180 0.0 41.249355 32 CGTATGC 190 0.0 40.262527 41 TATGCCG 190 0.0 40.262527 43 CCCACGA 190 0.0 40.262527 19 TATCTCG 190 0.0 40.262527 36 ACGAGAC 190 0.0 40.262527 22 CCACGAG 190 0.0 40.262527 20 ACTGCGA 185 0.0 40.134506 27 CTGCGAC 185 0.0 40.134506 28 CCTATCT 185 0.0 40.134506 34 GACTGCG 185 0.0 40.134506 26 TGCCGTC 185 0.0 40.134506 45 CTCGTAT 195 0.0 39.230152 39 TCCGAGC 195 0.0 39.230152 13 CACGAGA 195 0.0 39.230152 21 GTATGCC 195 0.0 39.230152 42 >>END_MODULE