FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_36.34100000006a54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_36.34100000006a54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70867
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC10311.4548379358516657No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.21307519720038948No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.14675377820424174No Hit
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT920.12982064994990616Illumina Paired End PCR Primer 2 (95% over 21bp)
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT900.1269984619075169No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA800.11288752169557056No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA206.9647515E-445.0310751
GCGACCT1800.041.24935530
CGACCTA1800.041.24935531
TGCGACC1800.041.24935529
GACCTAT1800.041.24935532
CGTATGC1900.040.26252741
TATGCCG1900.040.26252743
CCCACGA1900.040.26252719
TATCTCG1900.040.26252736
ACGAGAC1900.040.26252722
CCACGAG1900.040.26252720
ACTGCGA1850.040.13450627
CTGCGAC1850.040.13450628
CCTATCT1850.040.13450634
GACTGCG1850.040.13450626
TGCCGTC1850.040.13450645
CTCGTAT1950.039.23015239
TCCGAGC1950.039.23015213
CACGAGA1950.039.23015221
GTATGCC1950.039.23015242