##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_35.34100000006a47.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 104492 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.308061861195114 38.0 38.0 38.0 32.0 38.0 2 36.32717337212418 38.0 38.0 38.0 32.0 38.0 3 36.290806951728364 38.0 38.0 38.0 32.0 38.0 4 36.408193928721815 38.0 38.0 38.0 32.0 38.0 5 36.46037974198982 38.0 38.0 38.0 32.0 38.0 6 38.33701144585231 40.0 38.0 40.0 38.0 40.0 7 38.24984687822991 40.0 38.0 40.0 38.0 40.0 8 38.219748880297054 40.0 38.0 40.0 38.0 40.0 9 38.24449718638748 40.0 38.0 40.0 38.0 40.0 10 38.178118899054475 40.0 38.0 40.0 38.0 40.0 11 38.19318225318685 40.0 38.0 40.0 38.0 40.0 12 38.077288213451745 40.0 38.0 40.0 38.0 40.0 13 38.163601041228034 40.0 38.0 40.0 38.0 40.0 14 38.04435746277227 40.0 38.0 40.0 38.0 40.0 15 38.07190024116679 40.0 38.0 40.0 38.0 40.0 16 38.082484783524094 40.0 38.0 40.0 38.0 40.0 17 38.06901006775638 40.0 38.0 40.0 38.0 40.0 18 38.0996918424377 40.0 38.0 40.0 38.0 40.0 19 38.104850132067526 40.0 38.0 40.0 38.0 40.0 20 38.115166711327184 40.0 38.0 40.0 38.0 40.0 21 38.11232438846993 40.0 38.0 40.0 38.0 40.0 22 38.12245913562761 40.0 38.0 40.0 38.0 40.0 23 38.08194885732879 40.0 38.0 40.0 38.0 40.0 24 38.09513646977759 40.0 38.0 40.0 38.0 40.0 25 38.09352869119167 40.0 38.0 40.0 38.0 40.0 26 38.033064732228304 40.0 38.0 40.0 32.0 40.0 27 38.01980055889446 40.0 38.0 40.0 38.0 40.0 28 37.970342227156145 40.0 38.0 40.0 32.0 40.0 29 37.95956628258622 40.0 38.0 40.0 32.0 40.0 30 37.95066607969988 40.0 38.0 40.0 32.0 40.0 31 37.95871454274011 40.0 38.0 40.0 32.0 40.0 32 37.927190598323314 40.0 38.0 40.0 32.0 40.0 33 37.900710102208784 40.0 38.0 40.0 32.0 40.0 34 37.92362094705815 40.0 38.0 40.0 32.0 40.0 35 37.90758144164147 40.0 38.0 40.0 32.0 40.0 36 37.87796194924013 40.0 38.0 40.0 32.0 40.0 37 37.87962714848984 40.0 38.0 40.0 32.0 40.0 38 37.870726945603494 40.0 38.0 40.0 32.0 40.0 39 37.84920376679555 40.0 38.0 40.0 32.0 40.0 40 37.83124066914213 40.0 38.0 40.0 32.0 40.0 41 37.84873483137465 40.0 38.0 40.0 32.0 40.0 42 37.80614783906902 40.0 38.0 40.0 32.0 40.0 43 37.803123684109785 40.0 38.0 40.0 32.0 40.0 44 37.76766642422386 40.0 38.0 40.0 32.0 40.0 45 37.726160854419476 40.0 38.0 40.0 32.0 40.0 46 37.68333460934809 40.0 38.0 40.0 32.0 40.0 47 37.6654097921372 40.0 38.0 40.0 32.0 40.0 48 37.60820924089882 40.0 38.0 40.0 32.0 40.0 49 37.5963327336064 40.0 38.0 40.0 32.0 40.0 50 37.5728476821192 40.0 38.0 40.0 32.0 40.0 51 37.14570493434904 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.2692654227237554 2201 2 0.6055269422920588 2201 3 -0.23143146477579535 2201 4 -0.038291223427940224 2201 5 -0.08601768278072086 2201 6 -0.24314791861677065 2201 7 -0.06242765147990781 2201 8 -0.3858460729460944 2201 9 -0.005310189867927306 2201 10 -0.18645852299416532 2201 11 -0.13263514918091346 2201 12 0.14017448532208476 2201 13 -0.3284965108701243 2201 14 -0.11517912147772336 2201 15 0.08558684145679507 2201 16 0.21535994985531914 2201 17 0.16942804205697115 2201 18 -0.29595541670914827 2201 19 0.35186459723404084 2201 20 0.43666662199029105 2201 21 -0.21370455006415057 2201 22 0.17923906227120057 2201 23 -0.1184211970740705 2201 24 -0.12776649658269434 2201 25 0.27827583997392935 2201 26 0.43909677964964544 2201 27 0.09207317467111409 2201 28 -0.3941440908080196 2201 29 -0.010527579997557268 2201 30 -0.22279571885999871 2201 31 -0.1628779378438665 2201 32 0.7083274781867317 2201 33 0.5989406008093994 2201 34 -0.03447135184943306 2201 35 -0.059079318431727756 2201 36 -0.15724040094301017 2201 37 -0.07271644004826783 2201 38 0.10034738898144724 2201 39 0.23727392116762047 2201 40 0.5272581558765239 2201 41 0.3868696082406231 2201 42 0.22071201728510914 2201 43 -0.0948599539641819 2201 44 0.2063867221898974 2201 45 0.15213375018271336 2201 46 0.22571823767722776 2201 47 0.1417830236117723 2201 48 -0.8035609762269047 2201 49 -0.32664178458085047 2201 50 -0.34392851786057577 2201 51 -0.805057712222208 1101 1 -0.1625987560570934 1101 2 -0.3411397243746066 1101 3 0.2885685352242007 1101 4 0.4017087765720575 1101 5 -0.15268434944739084 1101 6 0.2901854147165679 1101 7 0.4442390151867599 1101 8 0.1341539270539016 1101 9 0.27468981013207383 1101 10 0.20687481033916555 1101 11 0.3206981841524197 1101 12 0.36684115198875134 1101 13 0.664836822463208 1101 14 0.4514875451889466 1101 15 0.5722535081234668 1101 16 -0.3046400501446769 1101 17 0.3094280420569717 1101 18 0.5373779166241874 1101 19 0.28519793056737797 1101 20 0.3299999553236219 1101 21 0.37962878326918315 1101 22 0.48590572893787254 1101 23 0.41491213625926093 1101 24 0.43223350341730793 1101 25 0.4916091733072676 1101 26 0.7257634463163072 1101 27 0.14540650800444155 1101 28 0.2791892425253124 1101 29 0.4694724200024396 1101 30 0.5038709478066679 1101 31 0.63045539548947 1101 32 0.08166081152006655 1101 33 0.5656072674760679 1101 34 0.7188619814839043 1101 35 0.6742540149016065 1101 36 0.4227595990569881 1101 37 -0.09271644004826385 1101 38 0.37368072231478067 1101 39 0.10394058783428761 1101 40 0.6472581558765214 1101 41 0.19353627490728798 1101 42 0.5740453506184409 1101 43 0.6651400460358161 1101 44 0.2997200555232311 1101 45 0.2854670835160462 1101 46 0.1657182376772255 1101 47 0.5217830236117749 1101 48 0.14977235710642844 1101 49 0.16669154875248182 1101 50 0.01607148213943077 1101 51 -0.10505771222221227 2202 1 0.18191737297516397 2202 2 0.2266022111092667 2202 3 0.32276208361129477 2202 4 0.19525716366883472 2202 5 0.08731565055261115 2202 6 0.31061552224344524 2202 7 0.37800245604697125 2202 8 0.45071306683885126 2202 9 0.5166252940030418 2202 10 0.7036490038875556 2202 11 0.6667196895287617 2202 12 0.03501319499950739 2202 13 0.23150348912987084 2202 14 0.30611120110292234 2202 15 0.1513932930697024 2202 16 0.46331693910263283 2202 17 -0.04691604396452931 2202 18 0.5083456585596693 2202 19 0.464982876803937 2202 20 0.3588171596246994 2202 21 0.45833846068853745 2202 22 0.2985939009808831 2202 23 0.6379228889474362 2202 24 0.5709431808366645 2202 25 0.5234371302965144 2202 26 0.430279575348564 2202 27 0.8950839273592806 2202 28 -0.012423660700498829 2202 29 -0.16149532193304594 2202 30 0.024946216623874307 2202 31 0.45755216968301937 2202 32 0.1980048975415727 2202 33 0.47937070833628326 2202 34 -0.1484498464730848 2202 35 -0.04897179155000231 2202 36 0.32749078185268843 2202 37 0.5242728072635643 2202 38 0.017981797583594528 2202 39 0.14286531901708344 2202 40 0.22983880103781473 2202 41 0.40256853297180584 2202 42 0.3460883613711303 2202 43 0.5911615514121635 2202 44 0.4902576899318305 2202 45 0.2067574060966919 2202 46 0.41432038821486117 2202 47 0.20565399135370654 2202 48 0.214073432375244 2202 49 -0.19352350501095827 2202 50 -0.28113281893584485 2202 51 -0.6173157767383373 1102 1 0.23022175676342016 1102 2 -0.020626903861789003 1102 3 0.21651725317291692 1102 4 0.19145236631565155 1102 5 0.6796233428603031 1102 6 0.3389033634345111 1102 7 0.1498800408277816 1102 8 0.27953854243852305 1102 9 0.18494622038848263 1102 10 0.024823528287882368 1102 11 0.6230058764601125 1102 12 0.7776103827579846 1102 13 0.28022143784782116 1102 14 0.10507728877868772 1102 15 0.2717406876106452 1102 16 -0.05310158860621783 1102 17 -0.13723862460969372 1102 18 0.5104548397011044 1102 19 0.38417228954173765 1102 20 0.3810255963492608 1102 21 0.21065442429482317 1102 22 0.7446236776558166 1102 23 0.30004034138746505 1102 24 0.11813093931474583 1102 25 0.49366045535854397 1102 26 0.2990967796496449 1102 27 0.25463727723521146 1102 28 0.5327789861150549 1102 29 0.8979339584639803 1102 30 0.6597683837041046 1102 31 0.21148103651511008 1102 32 0.31909670895596776 1102 33 0.028171370040169563 1102 34 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20 -0.11934187057871526 2203 21 0.3992466176640832 2203 22 0.5161817374304363 2203 23 0.24671680717221278 2203 24 0.37172394927718244 2203 25 0.4642631011204301 2203 26 0.48805643994688097 2203 27 0.5268077818897936 2203 28 0.5805905164106591 2203 29 -0.06250210229055142 2203 30 0.018987720630448734 2203 31 0.4398821470818248 2203 32 -0.09638589760944427 2203 33 0.6145669277733035 2203 34 0.1914309836070487 2203 35 0.697566116812439 2203 36 0.5089167115835309 2203 37 0.2059247489113929 2203 38 0.5419185142468379 2203 39 0.6647473818894909 2203 40 0.6550288565134679 2203 41 0.29009678446143283 2203 42 0.3303086202575045 2203 43 0.7514245470973862 2203 44 0.5184886330179239 2203 45 0.3091613510319675 2203 46 0.1322575158088597 2203 47 0.5829295204270579 2203 48 0.2603031426690663 2203 49 0.6102159648883614 2203 50 0.5364961105895318 2203 51 0.5219486572045398 1103 1 -0.1061281678217938 1103 2 0.3974223671286623 1103 3 0.18647703195622967 1103 4 0.13112054127794437 1103 5 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#Quality Count 15 3.0 16 1.0 17 1.0 18 0.0 19 0.0 20 4.0 21 9.0 22 30.0 23 62.0 24 138.0 25 227.0 26 361.0 27 615.0 28 844.0 29 1122.0 30 1405.0 31 1831.0 32 2135.0 33 2691.0 34 3362.0 35 4124.0 36 5755.0 37 8784.0 38 16487.0 39 54501.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.041166052367046 19.618974678091046 12.934756593748503 27.4051026757934 2 14.776392225167717 21.184599630590196 39.588098496521226 24.450909647720856 3 19.71921295410175 23.815220303946713 29.062511962638286 27.403054779313248 4 13.586686062090877 17.414730314282433 36.38173257282854 32.61685105079815 5 14.290089193431077 36.31378478735214 34.78161007541247 14.61451594380431 6 31.94407227347548 37.18753588791486 17.092217586035293 13.77617425257436 7 28.064349423879342 32.086666921869615 20.935574015235616 18.913409639015427 8 24.1386900432569 37.49760747234238 20.332657045515447 18.031045438885272 9 24.719595758526967 18.016690272939556 21.035103165792595 36.22861080274088 10 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20.96045630287486 23 21.1470734601692 28.37154997511771 28.469165103548598 22.01221146116449 24 22.029437660299354 27.744707728821343 28.96968188952264 21.25617272135666 25 21.446617922903187 28.311258278145697 28.896949048731003 21.345174750220114 26 21.307851318761244 28.45576694866593 29.080695172836197 21.15568655973663 27 21.334647628526586 28.98403705546836 28.48734831374651 21.193967002258546 28 21.20736515714122 28.45768097079202 28.097844811086013 22.237109060980746 29 21.744248363511083 28.12846916510355 28.76201048884125 21.36527198254412 30 21.73625931916278 27.82589554881375 28.600139727626832 21.837705404396633 31 22.113654633847567 27.8317957355587 28.20981510546262 21.84473452513111 32 21.81698120430272 28.57156528729472 28.093059755770778 21.51839375263178 33 21.213107223519504 28.86632469471347 28.810818053056693 21.10975002871033 34 21.556674195153697 28.072005512383726 29.152471002564788 21.21884928989779 35 21.316464418328675 28.559124143475103 28.535198866898902 21.589212571297324 36 21.16429965930406 28.32657045515446 29.38406767982238 21.125062205719097 37 21.443746889714042 28.042338169429236 28.311258278145697 22.20265666271102 38 21.375799104237643 28.2854189794434 28.55051104390767 21.788270872411285 39 21.820809248554912 27.62316732381426 28.59070550855568 21.965317919075144 40 22.15289208743253 27.52746621750947 29.140986869808216 21.17865482524978 41 22.046663859434215 27.118822493588024 28.854840561956895 21.979673085020863 42 21.977759062894766 27.694943153542855 28.79359185392183 21.533705929640547 43 21.20162309076293 27.826053669180418 29.094093327718866 21.878229912337787 44 20.95662825862267 28.39643226275696 28.57730735367301 22.069632124947365 45 20.795850400030623 28.10262986640126 29.18883742296061 21.91268231060751 46 21.643762201891054 27.281514374306166 28.858668606209086 22.21605481759369 47 21.674386555908587 27.22696474371244 28.38686215212648 22.711786548252498 48 20.80254947747196 27.744707728821343 28.67587949316694 22.776863300539755 49 21.94330666462504 27.127435593155457 28.420357539333153 22.508900202886345 50 20.87145427401141 28.294032079010833 28.46150901504421 22.373004631933547 51 20.804861709254475 27.84476983443392 28.26012058570198 23.09024787060963 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 226.0 1 127.5 2 29.0 3 59.0 4 89.0 5 85.0 6 81.0 7 101.0 8 121.0 9 100.5 10 80.0 11 165.0 12 250.0 13 424.5 14 599.0 15 664.5 16 730.0 17 588.5 18 447.0 19 374.0 20 301.0 21 386.5 22 472.0 23 393.5 24 315.0 25 416.5 26 770.0 27 1022.0 28 1402.5 29 1783.0 30 2508.0 31 3233.0 32 4059.0 33 4885.0 34 5728.0 35 6571.0 36 6843.0 37 7115.0 38 7178.5 39 7242.0 40 7266.0 41 7290.0 42 7237.0 43 7184.0 44 7308.0 45 7432.0 46 7317.5 47 7203.0 48 7133.0 49 7063.0 50 6748.5 51 6434.0 52 6295.5 53 6157.0 54 5896.0 55 5635.0 56 4908.0 57 4181.0 58 3695.5 59 3210.0 60 2762.5 61 2315.0 62 1968.5 63 1622.0 64 1351.0 65 1080.0 66 886.5 67 693.0 68 539.5 69 386.0 70 297.5 71 209.0 72 184.5 73 160.0 74 119.5 75 54.0 76 29.0 77 18.5 78 8.0 79 8.0 80 8.0 81 5.5 82 3.0 83 2.5 84 2.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03540940933277189 2 9.570110630478889E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0028710331891436663 12 0.0 13 0.0 14 0.0 15 9.570110630478889E-4 16 0.0028710331891436663 17 0.0 18 0.0019140221260957777 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0028710331891436663 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0019140221260957777 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 104492.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.00815056249071 #Duplication Level Percentage of deduplicated Percentage of total 1 93.6102795290453 83.32077854518106 2 4.985649719875208 8.875269218369843 3 0.7936531961581181 2.1192480953413124 4 0.25603338188978914 0.9115623121708013 5 0.11757464236151806 0.5232550734822499 6 0.05226856699184541 0.2791397088297485 7 0.029378848822843372 0.18304698995634067 8 0.035567470057613966 0.2532635784011987 9 0.018443939325082388 0.1477494835571139 >10 0.087162801364323 1.3770932763225818 >50 0.0064716913551429375 0.3887230035328825 >100 0.007516212753205898 1.6208707148548842 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 468 0.447881177506412 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 396 0.37897638096696395 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 226 0.2162845002488229 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 213 0.20384335642920035 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 149 0.14259464839413544 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 142 0.13589557095280022 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 9.570110630478889E-4 18 0.0 0.0 9.570110630478889E-4 19 0.0 0.0 0.0019140221260957777 20 0.0 0.0 0.0028710331891436667 21 0.0 0.0 0.0028710331891436667 22 0.0 0.0 0.0028710331891436667 23 0.0 0.0 0.0038280442521915555 24 0.0 0.0 0.011484132756574667 25 0.0 0.0 0.011484132756574667 26 0.0 0.0 0.015312177008766222 27 0.0 0.0 0.020097232324005666 28 0.0 0.0 0.03253837614362822 29 0.0 0.0 0.051678597404585996 30 0.0 0.0 0.08708800673735788 31 0.0 0.0 0.18278911304214676 32 0.0 0.0 0.29667342954484555 33 0.0 0.0 0.3942885579757302 34 0.0 0.0 0.5091298855414769 35 0.0 0.0 0.6344983348007504 36 0.0 0.0 0.7952761933927956 37 0.0 0.0 1.0029475940741874 38 0.0 0.0 1.2967499904298894 39 0.0 0.0 1.6403169620640814 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGAGC 50 1.0568328E-9 40.51595 13 ACCTATC 50 1.0622898E-9 40.496555 33 TATCTCG 50 1.0622898E-9 40.496555 36 TATGCCG 45 1.9010258E-8 39.996597 43 TGCCGTC 45 1.9010258E-8 39.996597 45 CCGAGCC 55 2.6884663E-9 36.83268 14 GCCCACG 55 2.7011993E-9 36.81505 18 ACTGCGA 55 2.7011993E-9 36.81505 27 GACTGCG 55 2.7011993E-9 36.81505 26 CCACGAG 55 2.7011993E-9 36.81505 20 GACCTAT 55 2.7011993E-9 36.81505 32 TCTCGTA 50 4.8103175E-8 35.996937 38 TCCACTA 25 0.0020992456 35.996937 26 TCGTATG 50 4.8103175E-8 35.996937 40 AAGTGTC 25 0.0020992456 35.996937 7 ATGCCGT 45 8.6054024E-7 34.99702 44 ATCTCCG 60 6.2900654E-9 33.76329 10 ATCTCGT 60 6.319169E-9 33.747128 37 CTATCTC 60 6.319169E-9 33.747128 35 CGACCTA 60 6.319169E-9 33.747128 31 >>END_MODULE