FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_35.34100000006a47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_35.34100000006a47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104492
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC4680.447881177506412No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3960.37897638096696395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2260.2162845002488229No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2130.20384335642920035No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1490.14259464839413544No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.13589557095280022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC501.0568328E-940.5159513
ACCTATC501.0622898E-940.49655533
TATCTCG501.0622898E-940.49655536
TATGCCG451.9010258E-839.99659743
TGCCGTC451.9010258E-839.99659745
CCGAGCC552.6884663E-936.8326814
GCCCACG552.7011993E-936.8150518
ACTGCGA552.7011993E-936.8150527
GACTGCG552.7011993E-936.8150526
CCACGAG552.7011993E-936.8150520
GACCTAT552.7011993E-936.8150532
TCTCGTA504.8103175E-835.99693738
TCCACTA250.002099245635.99693726
TCGTATG504.8103175E-835.99693740
AAGTGTC250.002099245635.9969377
ATGCCGT458.6054024E-734.9970244
ATCTCCG606.2900654E-933.7632910
ATCTCGT606.319169E-933.74712837
CTATCTC606.319169E-933.74712835
CGACCTA606.319169E-933.74712831